Labshake search
Citations for Beckman :
651 - 700 of 952 citations for Methyl 2 trifluoromethylsulfonyloxy 1 naphthoate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... resuspended in fresh DPBS buffer containing 1% FBS and analyzed on a CytoFLEX S flow cytometer (Beckman Coulter). Dead cells were excluded by 4′,6-diamidino-2-phenylindole (DAPI).
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bioRxiv - Immunology 2024Quote: ... Cells were then washed with 1×PBS and analyzed using a CytoFLEX Flow Cytometer (Beckman Coulter, Miami, FL) or sorted on a BD FACS Aria II ...
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bioRxiv - Biochemistry 2024Quote: ... The mixture was centrifuged at 55,000 rpm for 1 hr in Optima™ MAX-TL Ultracentrifuge (BECKMAN COULTER). Then the pellet was dissolved with 200 μL 1X Sample buffer ...
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bioRxiv - Cancer Biology 2024Quote: ... followed by washing with 1 ml of PBS before the analysis on the CytoFLEX LX (Beckman Coulter, Indianapolis). Antibodies used are the following ...
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bioRxiv - Developmental Biology 2023Quote: ... Peptide supernatant was cleared by ultracentrifugation at 100,000□g for 1 hour at 4□°C (Beckman Coulter, 343775), then vacuum-dried overnight.
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bioRxiv - Genomics 2024Quote: ... Target sequences were then amplified using specific primers (Table 1) and purified using AMPure beads (Beckman Coulter; A63880) at concentrations appropriate to each of the target sizes ...
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bioRxiv - Genomics 2023Quote: ... The DNA was purified from the lysated using a 0.9:1 ratio of Ampure XP Beads (Beckman Coulter) and eluted into 10 μl of water ...
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bioRxiv - Biophysics 2023Quote: ... the supernatant was layered onto PBS/1.5 M sucrose solution in sample/sucrose 4:1 ratio and ultracentrifuged at 264,000 × g on Optima TLA 100.3 ultracentrifuge (Beckman for 2 h at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... The remaining viral supernatant was centrifuged for 1 h at 25,000 rpm in an SW-28 rotor (Beckman). The resulting virus pellets were resuspended in 1-2 pellet volumes PBS and supplemented with 2.5 mM DSSO (100 mM stock solution in DMSO) ...
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bioRxiv - Microbiology 2022Quote: ... a fluorescent labeling was first made with anti-p24 antibody (Clone: KC57, Beckman Coulter, 6604667; 1/200 dilution). Then ...
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bioRxiv - Cell Biology 2023Quote: ... 10% and 6% and ultracentrifuged at 187,000xg for 1:30h at 4°C in Sw32.1Ti rotor (Beckman Coulter). Afterwards ...
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bioRxiv - Bioengineering 2024Quote: ... Cells were then washed once and resuspended with 1% paraformaldehyde and analyzed on a CytoFlex S (Beckman Coulter) flow cytometer ...
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bioRxiv - Developmental Biology 2024Quote: ... Insoluble material was removed by high speed centrifugation (30,000g, 1 hour, at 4°C, in rotor JA12, Beckman) and by 0.45 µm filtration ...
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bioRxiv - Immunology 2024Quote: Cells were washed 1× with FACS buffer after staining and acquired on a CytoFLEX S cytometer (Beckman Coulter).
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bioRxiv - Systems Biology 2024Quote: The stained 1 in 3 cell solution was assessed by flow cytometry (CytoFlex S Flow Cytometer, Beckman Coulter) and the number of singlet (based on FSC-H vs FSC-A) ...
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bioRxiv - Molecular Biology 2024Quote: ... 1 mM DTT) and the ribosomes were pelleted by centrifugation in a SW 41 Ti rotor (Beckman Coulter) at 38,000 rpm for 2.5 hours at 4°C ...
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bioRxiv - Plant Biology 2024Quote: ... Cultures containing 1-3 x 106 cells/mL were collected at 1,670 xg (JLA-10.500 rotor, Beckman Coulter). Cells were washed once (D ...
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bioRxiv - Immunology 2021Quote: ... SSM liquid culture was incubated for 24 hours at 36°C with constant shaking at 180 rpm until reaching OD600nm 0.5 with 1 cm path width (Beckman Coulter™ DU 530 UV Vis spectrophotometer) ...
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bioRxiv - Genomics 2020Quote: ... The sequencing libraries were cleaned up using AMPure XP beads (1:1.8 sample vol. to beads vol.; Beckman Coulter). The size distribution of the libraries were determined by Bioanalyzer (Agilent Technologies) ...
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bioRxiv - Biochemistry 2021Quote: ... and resuspended in 500 μL of 1% FBS/DPBS and transferred to polystyrene tubes for flow cytometric analysis (Beckman Coulter HyperCyAn ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were resuspended in p24 primary antibody solution (1:100 dilution of p24-FITC antibody clone KC57 (Beckman Coulter) diluted in perm/wash buffer ...
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bioRxiv - Biophysics 2020Quote: ... and centrifuged at 287,582 x g (90,000 rpm) for 1 hr at 4 °C in a TLA100.2 rotor (Beckman, ref. 362046) in 11×34 mm thick-wall polycarbonate ultracentrifuge tubes (Beckman ...
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bioRxiv - Neuroscience 2022Quote: ... The samples were separated through ultracentrifugation for 1 hr at 200,000 g (spin 3 at 49,000 r.p.m. in Beckman TLS-55 rotor). Top layers of supernatants were removed ...
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bioRxiv - Microbiology 2022Quote: ... Cells were then incubated with the fluorescently labeled anti-p24 antibody (Clone: KC57, Beckman Coulter, 6604667; 1/200 dilution).
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bioRxiv - Biochemistry 2022Quote: ... 1 mM DTT using a MLA-50 rotor in an Optima MAX-XP Ultracentrifuge (Beckman Coulter, Pasadena, California, USA) at 180.000 g for 5 h at 4 °C ...
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bioRxiv - Biochemistry 2022Quote: ... The crude extract was centrifuged at 200,000 g for 1 h at 4 °C (Beckman Coulter Optima LE-80K). The membrane pellet was homogenized and solubilized in 20 mM HEPES (pH 7.8 ...
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bioRxiv - Cancer Biology 2022Quote: ... Amplicons from up to 3,072 libraries were pooled and purified with Ampure XP beads (0.8:1 ratio beads to product; Beckman Coulter). These steps were performed using Biomek FxP (Beckman Coulter) ...
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bioRxiv - Microbiology 2021Quote: ... buoyant density of 1.71 g ml−1) in polyallomer tubes before sealing and ultracentrifugation at 152,000 × g (50,000 rpm) in a MLN80 rotor (Beckman-Coulter) for 72 h at 25°C ...
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bioRxiv - Microbiology 2021Quote: ... cells were diluted in 50 mM filtered TrisHCl pH 7.5 to 106 mL-1 and analysed using a cytofluorimeter (CytoflexS, Beckman) with excitation and emission wavelength of 488 and 525 nm respectively ...
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bioRxiv - Biochemistry 2021Quote: ... Reaction mixtures were incubated for 30 minutes and centrifuged at 100,000g at 4°C for 1 hr using a TLA100 fixed angle rotor (Beckman). The supernatant fraction was carefully removed ...
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bioRxiv - Immunology 2020Quote: ... 10 mM Tris-HCl, pH 8.1), chromatin was eluted (1% SDS, 0.1M NaHCO3)and recovered with Agencourt AMPure XP beads (Beckman Coulter). Bound chromatin and input DNA were treated with RNase H (New England Biolabs ...
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Small Molecule Screen Identifies Pyrimethamine as an Inhibitor of NRF2-driven Esophageal HyperplasiabioRxiv - Cancer Biology 2022Quote: ... Individual compounds (1 µM in 0.1% DMSO) were added to each well using a Biomek NX workstation (Beckman-Coulter)(1 well per compound) ...
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bioRxiv - Cell Biology 2023Quote: ... and 1 mM PMSF) and centrifuged for 15 hours at 4°C with 74,400 xg (SW 32.1 Ti; Beckman Coulter). 24 equal volume fractions were collected from the top and subjected to TCA precipitation ...
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bioRxiv - Plant Biology 2023Quote: ... It was then centrifuged again at 100,000 x g for 1 h at 4 ⁰C (TLA 55 rotor, Beckman Coulter ...
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bioRxiv - Biophysics 2023Quote: ... The supernatant was subjected to a second round of ultracentrifugation at 100,000 x g for 1 hour at 4°C in a Type 45Ti rotor (Beckman) to pellet cell membranes ...
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bioRxiv - Plant Biology 2023Quote: ... The tubes were then centrifuged at 100,000 x g for 1 h at 4 ⁰C (TLA 55 rotor, Beckman Coulter ...
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bioRxiv - Neuroscience 2022Quote: ... Then the lysates were spin down at 67,000 rpm for 1 hour at 18 °C with the ultracentrifuge (Beckman). The AAV particles were collected by collecting the interface between 40 and 60% iodixanol with a syringe ...
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bioRxiv - Developmental Biology 2023Quote: ... Double size selection to remove primer dimers and fragments exceeding 1 kb was performed using SPRIselect beads (Beckman Coulter). Another quality control was performed with High Sensitivity DNA assay using the Fragment Analyzer (Agilent) ...
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bioRxiv - Molecular Biology 2024Quote: ... for ultracentrifugation at 100,000 × g for 1 hour at 4°C (JXN-30 centrifuge, JA-30.50 Ti Rotor, Beckman Coulter). The pellet was washed with PBS and centrifuged again at 100,000 × g for 1 hour at 4°C to remove soluble contaminant ...
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bioRxiv - Molecular Biology 2024Quote: ... and 1 M sucrose) at 100,000 rpm (543,000 × g) and 4°C for 1 h with a TLA110 rotor (Beckman Coulter) and an Optima MAX-TL Ultracentrifuge (Beckman Coulter) ...
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bioRxiv - Developmental Biology 2023Quote: ... Size selection and purification was achieved by double-sided (0.65 × −1× volume) using SPRIselect magnetic beads (Beckman Coulter, B23318). Concentrations were measured using the Qubit HS dsDNA assay ...
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bioRxiv - Evolutionary Biology 2024Quote: ... DNA was enriched in samples with low DNA concentration (1-60 ng/μl) using AMPureXP Beads (SPRIselect, Beckman Coulter) in a 1:1 ratio ...
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bioRxiv - Neuroscience 2023Quote: ... NP-40 prepared in DPBS] and then ultracentrifuged at 100,000x g for 1 h at 4 °C using a TLA-55 rotor (Beckman). The supernatant was removed ...
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bioRxiv - Biochemistry 2023Quote: ... The mixture was incubated for 30 min at 4 °C and centrifuged at 100,000 × g at 4 °C for 1 h using a TLA100 fixed angle rotor (Beckman). The supernatant fraction was carefully removed ...
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bioRxiv - Cancer Biology 2022Quote: ... resuspended in 250 µl FACS buffer with 1 µl DAPI and analyzed with a Gallios flow cytometer (Beckman Coulter)
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bioRxiv - Cell Biology 2022Quote: ... were mixed 1:1 with liposomes (5 mg/ml) and floated for 2h at 55,000 rpm in a TLS-55 rotor (Beckman) at 25 °C through a sucrose gradient ...
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bioRxiv - Microbiology 2022Quote: ... 1 mM dithiothreitol and 100 μg/ml cycloheximide) and spun for 1 h at 100,000 rpm using TLA-110 rotor (Beckman) at 4 °C ...
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bioRxiv - Microbiology 2022Quote: ... the cross-linked material was loaded onto glycerol-tartrate gradients as described elsewhere 53 and centrifuged for 1 h at 25,000 rpm in an SW-40 rotor (Beckman), with brakes set at the slowest possible deceleration ...
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bioRxiv - Microbiology 2022Quote: ... The post-nuclear supernatant was adjusted to 0.8 M sucrose and loaded in the middle of a step gradient ranging from 0.1 to 1.8 M sucrose in STKM buffer and centrifuged at 50’000 g for 1 h at 4°C in a TLA-120.1 fixed-angle rotor (Beckman Coulter). LDs formed a white cushion on top of the tube and were collected by aspiration of the upper fraction of the gradient (125 µl).
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bioRxiv - Immunology 2023Quote: ... SSM liquid culture was incubated for 24 hours at 36°C with constant shaking at 180 rpm until reaching mid-log phase OD600nm 0.5 with 1 cm path width (Beckman Coulter™ DU 530 UV Vis spectrophotometer) ...