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Citations for Beckman :
651 - 700 of 767 citations for Marker 7 Pcb Mixture W Pcb 118 13C12 99% 100 Ng Ml In Nonane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Proteome of the secondary plastid of Euglena gracilis reveals metabolic quirks and colourful historybioRxiv - Evolutionary Biology 2019Quote: ... The DNase-treated lysate (5 ml) was loaded on top of a sucrose discontinuous density gradient in ultracentrifuge tubes (Cat. No. 344058, Beckman). The gradient was prepared by layering decreasingly dense sucrose solutions upon one another from 2.0 ...
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bioRxiv - Biophysics 2019Quote: Sedimentation velocity data of the N-terminal RBCC domain of KAP1 (0.5 mg/mL) were obtained using a Beckman Optima XL-I analytical ultracentrifuge (Beckman Coulter) with an absorbance optical system ...
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bioRxiv - Molecular Biology 2021Quote: ... Sucrose solutions were made in Buffer G and 25 x 89 mm 38.5 mL open-up polyallomer tubes were used (Beckman Coutler). Ultra-centrifugation was performed (160,000 x g ...
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bioRxiv - Microbiology 2020Quote: ... The lysate was transferred into precooled 250 mL centrifuge bottles and centrifuged at 20,000 rpm (Avanti® JE centrifuge and JLA-16.250 rotor from Beckman Coulter) for 15 min at 4°C.
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bioRxiv - Microbiology 2020Quote: ... through a 1 ml 30% sucrose cushion (in phosphate-buffered saline, PBS, pH 7.4) in a SW41 rotor (Beckman coulter). The pelleted virus material was re-solubilized in PBS and analyzed by sodium docecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE ...
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bioRxiv - Immunology 2021Quote: ... the pellets were resuspended in cold PBS and transferred to one Ultra-Clear 13.2 mL tube (Beckman Coulter, ref 344059) and centrifuged again at 100,000g 4°C for 1 hour and 30 minutes ...
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bioRxiv - Microbiology 2022Quote: ... 20 mL of the filtrate was then ultracentrifuged at 150,000 × g at 4°C for 3 h (Beckman Coulter, USA) through a 20% (w/v ...
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bioRxiv - Microbiology 2023Quote: ... buffer C containing 73% sucrose and buffer C containing 53% sucrose were layered in a 13 ml tube (Beckman Coulter), starting from the bottom ...
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bioRxiv - Cell Biology 2022Quote: ... 4–40% sucrose gradient prepared in lysis buffer and centrifuged at 150,000 × g for 4 or 16 h at 4°C in a MLS-50 rotor (Beckman Coulter). Thereafter ...
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bioRxiv - Cancer Biology 2022Quote: ... Fractions 2–9 were diluted 1:10 in PBS and centrifuged at 20,000 ×g (bEVs) or 100,000 ×g (sEVs) at 4°C for 1 h with an MLS-55 rotor (Beckman Coulter). The bEV and sEV pellets were suspended in double 0.22-µm-filtered PBS and were either used directly for experiments or stored in aliquots at −80°C until subsequent BLI ...
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bioRxiv - Cell Biology 2023Quote: ... The supernatant was then overlaid onto a 2 ml 60% iodixanol cushion and spun at 100,000 x g (SW32 rotor, Beckman Coulter) for 18h ...
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bioRxiv - Biochemistry 2023Quote: ... Eight 50 μL PCR reactions were combined in a 1.5 mL Eppendorf tube and mixed with 320 μL Ampure XP beads (Beckman Coulter) (0.8:1 ratio ...
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bioRxiv - Cell Biology 2023Quote: ... down to 6 ml and ultracentrifuged at 10,000xg for 16 min at 4°C in MLA-80 rotor (Beckman Coulter). The pellet was resuspended and washed with PBS at the same conditions (volume ...
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bioRxiv - Biophysics 2023Quote: ... followed by layering the cleared lysate onto 40 mL of 2 M sucrose cushion and ultracentrifugation in Ti-45 rotor (Beckman) at 42,000 rpm for 2 hours ...
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bioRxiv - Immunology 2023Quote: ... and pellet was resuspended and homogenised for final purification by sucrose cushion separation after ultracentrifugation in an MLS 50 rotor (Beckman). The final purified virus stock was diluted in TNE buffer (20 mM Tris-HCl pH 8 ...
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bioRxiv - Cell Biology 2023Quote: A 9-step discontinuous isosmotic iodixanol density gradient was prepared in a 13.2 mL open-top thinwall ultra-clear centrifugation tube (#344059, Beckman Coulter) using Optiprep and iodixanol buffer B (IBB ...
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bioRxiv - Microbiology 2023Quote: ... and 5.5 ml was layered on top of a two-step sucrose gradient consisting of 5 ml of 40% sucrose solution layered on top of 1.5 ml of a 65% sucrose solution in a 14 x 89mm Ultra-Clear tube (Beckman Coulter). All sucrose (w/w ...
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bioRxiv - Cell Biology 2024Quote: ... EVs were then placed in the bottom 36% layer of a 6-step gradient in a 13.2 ml open top polyallomer tube (331372, Beckman Coulter). A reference gradient was prepared in parallel and OptiPrep absorbance was measured at 340 nm to estimate the fraction density ...
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bioRxiv - Cell Biology 2022Quote: ... Yeast cells were stained with 5ug\ml propidium iodide for 10 minutes and samples were examined in CytoFlex flow cytometer (Beckman Coulter) using PE channel ...
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bioRxiv - Biophysics 2022Quote: ... in order to concentrate the medium to use the ultracentrifuge tubes with a reduced volume capacity for the small EV isolation (8.9 ml polypropylene centrifuge tube, Beckman Coulter, 361623). The tubes were filled with PBS to reach the final volume and samples were ultracentrifuged at 120,000 g for 60 minutes at 4°C (70.1 Ti rotor ...
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bioRxiv - Genomics 2020Quote: ... 300ul was transferred to a fresh 1.5-mL Eppendorf tube for two rounds of purification using AMPure XP paramagnetic beads (Beckman Coulter, Inc.) with ratios of 0.5X and 1.3X respectively ...
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bioRxiv - Neuroscience 2019Quote: ... with 2 ml of a 20% sucrose in dPBS cushion and ultracentrifugated at 20,000 RPM (Beckman Coulter SW 32 Ti rotor) at 4°C for 2 hours ...
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bioRxiv - Synthetic Biology 2021Quote: ... cells were diluted 100-fold in PBS to reach the final cell concentration of approximately 106 cfu/mL and measured on a CytoFLEX S (Beckman Coulter) using a FITC fluorescence channel with a 488 nm excitation laser and 525/40 nm emission band-pass filter with side scatter triggered events threshold set to 10,000 events ...
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bioRxiv - Microbiology 2020Quote: ... Whole blood staining was done with Alexa Fluor®647 labelled-VLP (2.5µg/ml) and cell subsets were discriminated using the following antibody panel: CD45 (Clone J33; Beckman Coulter, Brea, CA), CD11c (Clone BU15 ...
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bioRxiv - Microbiology 2021Quote: ... triplicate spore preparations were made in 3 ml of 20% glycerol and suspended in Isoton II (Beckman Coulter, Brea, CA, USA). The particle volume was then measured for a minimum of 100 000 particles per sample using a Multisizer 4e Coulter counter (Beckman Coulter ...
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bioRxiv - Microbiology 2021Quote: ... starting with 50% and topping off with 15% for a total of 11 mL was added to thin wall polypropylene ultracentrifuge tubes (Beckman Coulter). The tubes were immediately frozen and stored at -80 °C ...
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bioRxiv - Microbiology 2021Quote: ... the cell suspensions were diluted in 1 mL and loaded onto a cytoflex-LX flow cytometer (Beckman Coulter, Brea, CA, USA) with a 375 nm laser ...
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bioRxiv - Biophysics 2021Quote: ... and the fractions containing the protein of interest were combined and concentrated to 2mL at a final concentration of 3.85 mg/mL by repeated centrifugation in an Amicon Ultra Centrifugal Filter at 4k rpm for 5 min at 4 °C (Beckman SX4250). The concentrated protein was incubated overnight with TEV (1:50 mass ratio ...
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bioRxiv - Microbiology 2022Quote: A 3 ml volume of pseudoparticles was pelleted using a TLA-55 rotor with an Optima-MAX-E ultracentrifuge (Beckman Coulter) for 2 hours at 42,000 rpm at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... Most of the supernatant was discarded leaving 12x∼200 µL of concentrated fibers that were pooled and layered on top of ultracentrifuge tubes of 4 mL (polypropylene centrifuge tubes, Beckman Coulter) containing a discontinuous sucrose gradient (40% ...
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bioRxiv - Biochemistry 2022Quote: 1 mg of nuclear extract supplemented with protease inhibitors was carefully overlaid onto a 10 ml 10-30% glycerol gradient prepared in a polyallomer centrifuge tube (Beckman Coulter). Tubes were placed in a SW-41 swing bucket rotor and centrifuged at 4°C for 16 hours at 40,000 rpm ...
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bioRxiv - Microbiology 2022Quote: ... Washed pellets were resuspended in 2 mL 1x PBS and fluorescence was calculated using a LSR II cell analyzer (Beckman Coulter). Two biological replicates of 50,000 cell counts were analyzed with FlowJo ...
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bioRxiv - Cell Biology 2022Quote: ... A new supernatant was collected in a prechilled 1.5 ml ultracentrifuge tube and centrifuged at 100000 g for 1 h at 4°C in a TLA-55 rotor (Beckman Coulter). The resulting pellet was resuspended in the same isotonic buffer containing antiproteases and was frozen at -80°C together with the 100000 g supernatant ...
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bioRxiv - Cell Biology 2022Quote: ... which was then slowly poured on top of 10 ml cushion solution (2.05M sucrose with protease inhibitors, DTT and PMSF) in the ultracentrifugation tube (Beckman polyallomer #326823). After a 45-min spin at 24,600 rpm in pre-chilled SW32Ti rotor at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... A sucrose density gradient was prepared with 5 ml of both 0.8 M and 1.2 M sucrose in thin wall ultracentrifugation tubes (Beckman Coulter, Cat # 344059). P2 homogenate was mixed with 1 ml of homogenizing buffer ...
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bioRxiv - Molecular Biology 2020Quote: ... samples were filled into 38 mL open-top polypropylene tubes and subjected to DUC using a SW Type 32 Ti swinging bucket rotor (Beckman Coulter) in an Optima XPN-100 Ultracentrifuge (Beckman Coulter ...
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bioRxiv - Microbiology 2022Quote: ... Pellets were resuspended in 5 ml SM buffer and purified via CsCl density gradient ultracentrifugation (Optima XPN-80 ultracentrifuge; Beckman Coulter) at 19,200xg for 18 h at 10°C ...
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bioRxiv - Systems Biology 2020Quote: ... The resultant supernatant was collected and underwent ultracentrifugation at 100,000xg for 40 minutes at 4°C (rotor MLA-55) in 10.4 ml 16×76 mm polycarbonate capped centrifuge tubes (Beckman Coulter 355603), the pellet was washed once in PBS−/− and centrifuged again at 100,000xg for 40 minutes at 4°C ...
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bioRxiv - Biophysics 2019Quote: ... to final volume of 12 ml and then spun at 15°C for 16hr at 155,000g using a SW 41Ti rotor (Beckman Coulter, CA). The VLPs were detected at the lower part of the tube and recovered as described above ...
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bioRxiv - Cell Biology 2020Quote: ... 3 ml of 50% and 3 ml of 40% sucrose) in Ultra-Clear Beckman tubes and centrifuged at 26000 rpm (SW32Ti rotor, Beckman Coulter) for 1 h at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... The supernatant was ultracentrifuged at 120,000 g for 3 h at 4°C using an MLS-50 rotor (Beckman Coulter, USA) to collect 120k pellet vesicles ...
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bioRxiv - Systems Biology 2020Quote: ... each resultant fraction underwent separate ultracentrifugation at 100,000xg for 40 minutes at 4°C (rotor TLA 120.2) in 1 ml 11×34 mm polycarbonate uncapped centrifuge tubes (Beckman Coulter 343778). In EV-enriched pooled fraction experiments ...
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bioRxiv - Systems Biology 2020Quote: ... and underwent ultracentrifugation at 100,000xg for 40 minutes at 4°C (rotor MLA-55; Beckman Coulter 355603 10.4 ml capped tubes). Supernatant was discarded from each fraction ...
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bioRxiv - Cell Biology 2022Quote: ... A 12 ml 2 % step Optiprep gradient in LB ranging from 32 % to 10 % was prepared beforehand in Ultra-Clear tubes (Beckman Coulter). Samples were spun for 16 hours at 100000 g at 4 °C ...
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bioRxiv - Cell Biology 2022Quote: Whole bovine milk samples (200 mL) were centrifuged at 3,000 x g for 15 min at 4°C (Allegra X-15R, Beckman Coulter, France) to separate the fat from the skimmed milk ...
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bioRxiv - Immunology 2022Quote: ... cells were seeded into pre-coated wells with anti-TCR pan-γδ clone IMMU510 antibody (5ug/ml) (Beckman Coulter, Krefeld, Germany) and IL-2 (100IU/ml) ...
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bioRxiv - Molecular Biology 2022Quote: ... The gradients were prepared using freeze-thaw method42 in Thinwall Ultra-Clear ultracentrifuge tubes (5 ml, 13×51 mm; Beckman-Coulter). To create the 50% sucrose cushion ...
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bioRxiv - Microbiology 2022Quote: ... The recovered supernatant of both induced and uninduced cultures was transferred in 6 polyallomer (36 ml each) centrifuge tubes (25×89 mm, Beckman Coulter) to which 1 ml of Protease Inhibitor Cocktail (Sigma Aldrich ...
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bioRxiv - Plant Biology 2022Quote: ... extracts were loaded carefully into thick-walled polycarbonate tubes with three-piece caps (10.4 mL, Polycarbonate Bottle with Cap Assembly, 16 × 76 mm - 6Pk, 355603, Beckman Coulter, USA) pre-filled with 2.5 mL sucrose cushion (SC ...
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bioRxiv - Molecular Biology 2022Quote: ... Doubling times were determined by sub-culturing parasites repeatedly (over 5 days) to 106 cells/ml and measuring cell densities 24 hours later using a Coulter Counter Z2 particle counter (Beckman Coulter).