Labshake search
Citations for Beckman :
651 - 700 of 855 citations for 7 Oxa 3 azabicyclo 4.1.0 heptane 1 methyl 3 propyl 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2021Quote: ... The thylakoid membranes with different densities were analytically separated at 125,000 × g and 4°C for 1 h (SW 41 Ti, Beckman Coulter).
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bioRxiv - Microbiology 2021Quote: ... culture samples were diluted (1:100 or 1:1000) with 0.2-µm-filtered 18% BSW and were analysed with a Multisizer M4 Coulter cytometer (Beckman-Coulter) equipped with a 20 or 30 µm aperture tube ...
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bioRxiv - Microbiology 2020Quote: ... OBs were loaded on to a discontinuous 35–60% (w/w) sucrose gradient and centrifuged at 20000 rpm and 4°C for 1 hour in a SW41 rotor (Beckman). The band containing the OBs was removed ...
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bioRxiv - Cell Biology 2020Quote: ... After purification of the PCR product with a 1: 0.6 ratio of PCR product to AMPure XP beads (Beckman Coulter), the success of cDNA preparation was confirmed using a 2100 Bioanalyzer with High-sensitivity DNA chip (Agilent) ...
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bioRxiv - Neuroscience 2020Quote: ... The remaining supernatant (conditioned medium) was subjected to ultracentrifugation (UC) (100,000 x g 1 h) using rotors Ti45 or SW32Ti (Beckman Coulter). The pellet was rinsed with PBS and spun again using rotor SW55Ti (100,000 x g 1 h).
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bioRxiv - Neuroscience 2022Quote: ... The remaining supernatant (conditioned medium) was subjected to ultracentrifugation (UC) (100,000 x g, 1 h) using rotors Ti45 or SW32Ti (Beckman Coulter). The pellet was rinsed with PBS and spun again using rotor SW55Ti (100,000 x g ...
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bioRxiv - Neuroscience 2022Quote: ... 10 mM Tris-HCl) and centrifugated at 24,000 rpm for 1 h at 4°C in a SW41Ti Swinging-Bucket Rotor (Beckman Coulter). Supernatant was discarded and nuclei pellets were re-suspended in 800 µL of PBS ...
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bioRxiv - Biochemistry 2019Quote: ... 10,000 events were then immediately analyzed from 1 of the 4 150 µL aliquots using a CytoFLEX benchtop cytometer (Beckman). All subsequent chase time points were collected using this initial analysis as a reference ...
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bioRxiv - Biochemistry 2019Quote: ... The supernatant was then slowly mixed with an equal volume of 4 M ammonium sulphate (2 M final concentration) at 4°C for 1 hour and spun at 10 K rpm using a JA-17 rotor (Beckman). Pellets containing RAD51 were suspended in 25 ml of resuspension buffer (0.5 M KCl ...
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bioRxiv - Biophysics 2021Quote: ... Cells were lysed and the lysate was clarified by centrifugation for 1 h at 100,000g (45 Ti rotor, Beckman Coulter). The clarified lysate was incubated with Ni2+-NTA agarose resin for 1 h at 4 °C ...
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bioRxiv - Synthetic Biology 2021Quote: ... Libraries corresponding to samples to be directly compared to each other (e.g. DMSO vs UNC7040) were pooled together and purified using 1 volume of Agencourt AMPure XP (Beckman Coulter). The pooled libraries were eluted with 23 µL of elution buffer (NEXTflex ChIP-Seq kit ...
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bioRxiv - Immunology 2020Quote: ... Then the concentrated supernatant was ultracentrifuged at 110,000 g for 1 hour at 4°C (Beckman Coulter L100k, company name) to sediment the vesicular fraction into a pellet ...
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bioRxiv - Microbiology 2020Quote: ... The cell lysate was collected and centrifuged at 100,000 x g for 1 h in an ultracentrifuge (Model XL-90, Beckman Coulter). The supernatant was collected ...
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bioRxiv - Cancer Biology 2019Quote: ... All 384 single-cell libraries in each batch were pooled and cleaned up with 0.6:1 Ampure XP beads (Beckman Coulter). The multiplexed RNA-seq library was normalized to 4nM with Suspension Buffer (provided in Nextera XT kit ...
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Heatrich-BS enables efficient CpG enrichment and highly scalable cell-free DNA methylation profilingbioRxiv - Genomics 2021Quote: ... the sample was incubated at 25°C for 1 hour and then cleaned up by performing two rounds of 1.2x SPRI Select (Beckman Coulter). The sample was then subject to bisulfite conversion following the recommended protocol of Zymo EZ DNA Methylation kit (Zymo Research) ...
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bioRxiv - Genomics 2021Quote: ... The cDNA was purified using RNA XP beads purification with 1:1.8x ratio of sample to beads (Beckman Coulter, A63987) and eluted in 24.5 µl water ...
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bioRxiv - Neuroscience 2021Quote: ... and centrifuged at 250,000 g for 1 hour at room temperature with a TLA 120.2 rotor using an Optima XP benchtop ultracentrifuge (Beckman Coulter). Supernatant were collected by pipetting ...
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bioRxiv - Microbiology 2021Quote: ... bacterial cells were washed in PBS and diluted 1:5 before acquisition on a CytoFLEX benchtop flow cytometer (Beckman Coulter). For each sample ...
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bioRxiv - Genomics 2020Quote: ... This sequencing-ready NGS library was purified using 1 round of a 1X proportion of AMPure XP beads (Beckman Coulter). The final elution volume was 17 µl in Elution Buffer.
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bioRxiv - Microbiology 2021Quote: ... followed by purification with a 2.2:1 volumetric ratio of RNAClean XP solid phase reverse immobilization (SPRI) beads (A63987, Beckman Coulter). Copy numbers of resulting cDNAs were determined by limiting-dilution PCR using fluorescence-assisted clonal amplification (FCA ...
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bioRxiv - Microbiology 2020Quote: ... through a 1 ml 30% sucrose cushion (in phosphate-buffered saline, PBS, pH 7.4) in a SW41 rotor (Beckman coulter). The pelleted virus material was re-solubilized in PBS and analyzed by sodium docecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE ...
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bioRxiv - Immunology 2021Quote: ... Cells were stained with following antibody mix for 30 minutes on ice: pan-γδTCR-PE (1:10; IMMU510; Beckman Coultier) and CD69-APC (1:25 ...
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bioRxiv - Immunology 2021Quote: ... 1×106 thawed PBMCs were centrifuged (750×g for 5 minutes at 4°C) using a swinging bucket rotor (Beckman Coulter Avanti J-15RIVD with JS4.750 swinging bucket ...
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bioRxiv - Microbiology 2022Quote: ... PCR products were confirmed through visualization in 1% agarose gel and purified using AMPure XP beads (Beckman Coulter, Indianapolis, U.S.A.). PCR libraries were pooled together and sent to the Centre d’expertise et de services Genome Québec (Montréal ...
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bioRxiv - Cell Biology 2022Quote: ... lipoprotein particles (LPPs) were purified from the non-sedimentable fraction after 1 h of ultracentrifugation in a Ti-45 rotor (Beckman) at 44,000 rpm at 4 °C ...
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bioRxiv - Genetics 2022Quote: ... followed by 72°C for 1 minute followed by purification with 1X Agencourt AMPure XP beads as per the manufacturer’s instructions (Beckman Coulter).
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bioRxiv - Plant Biology 2022Quote: ... in a 12.5 mL ultracentrifuge tube and centrifuged for 1 h at 4°C at 200,000 × g in a swinging bucket rotor (Sorval SW-41 Ti; Beckman Coulter), with moderate acceleration and deceleration ...
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bioRxiv - Molecular Biology 2022Quote: ... and centrifuged at 15 000 RPM for 1 minute using an AllegraTM X-22R Centriuge (Beckman Coulter™, California, USA) to ensure all reagents were concentrated at the bottom of the wells ...
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bioRxiv - Molecular Biology 2022Quote: ... and purified according to the previously mentioned bead clean-up procedure (using 0.6 to 1-fold AMPure XP bead suspension, A63880, Beckman Coulter), except that after removal of ethanol ...
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bioRxiv - Molecular Biology 2022Quote: ... Transposition was blocked by the addition of 1% SDS and DNA purified with Agencourt AMPure XP beads (Beckman Coulter, A63881) according to the manufacturer’s instructions using a 2:1 ratio of beads to sample ...
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bioRxiv - Plant Biology 2024Quote: ... using a standard protocol but with all reaction volumes reduced by 1/10 to accommodate the automation of the prep on the Echo LabCyte liquid handler (Beckman). Libraries were purified using Agencourt Ampure XP beads (Beckman Coulter) ...
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bioRxiv - Microbiology 2023Quote: ... PBS-washed cells were PFA-fixed for 90 minutes and immunostained with the following antibodies diluted in 0.1% Triton X-100 in PBS: anti-HIV-1-core-antigen-FITC or -PE (#6604665 or #6604667; Beckman Coulter), rabbit-anti-human-MX1/2 (#sc-166412 ...
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bioRxiv - Molecular Biology 2024Quote: ... Lysates were cleared by centrifugation for 1 h at 45,000 rpm at 4°C (Optima XPN-80 Ultracentrifuge with 70 Ti, Beckman Coulter). HTP-tagged proteins were immunoprecipitated with 600 µl pre-washed IgG-sepharose 6 fast flow affinity resin (GE Healthcare ...
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bioRxiv - Neuroscience 2024Quote: ... The supernatant was ultracentrifuged at 80,000 × g for 1 h at 4°C using a type 50 Ti rotor ultracentrifuge (Beckman Coulter). The supernatant ...
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bioRxiv - Microbiology 2024Quote: ... Additional aliquots of seawater taken from the same CTD casts used for cell-specific rate incubations were 1) preserved in paraformaldehyde (0.5% final concentration) for later shore-based analyses on a CytoFlex S flow cytometer (Beckman Coulter) after staining with Hoechst 34580 (Selph 2021) ...
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bioRxiv - Microbiology 2022Quote: ... The eluted sample was collected by centrifugation at 2000 rpm for 1 minute 4 °C in a F241.5P rotor using a 149 Microfuge 22R centrifuge (Beckman Coulter). For SDS-PAGE analyses ...
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bioRxiv - Cell Biology 2023Quote: A sucrose gradient was prepared by mixing 3 ml of liver homogenate with 1 ml of HLM containing 60% sucrose in a 13.2 ml ultracentrifuge tube (Beckman Coulter), followed by 5 ml and then 4 ml of HLM buffer containing 5% and 0% sucrose ...
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bioRxiv - Microbiology 2023Quote: ... Not solubilised protein was removed by a second pelleting at 100×kg for 1 h in Ti70 rotor (Beckman Coulter). The solubilized and denatured protein was loaded on a 5 ml HisTrap HP column (Cytiva) ...
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bioRxiv - Molecular Biology 2023Quote: ... and ultracentrifuged in a sucrose cushion at 100,000 rpm at 4°C for 1 h with a TLA110 rotor (Beckman Coulter). The ribosome pellet was dissolved in TRIzol reagent (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2022Quote: ... DNase-treated RNA was subsequently purified using RNase-free Agencourt AMPure XP beads (Beckman Coulter; 2:1 volume of beads:sample).
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bioRxiv - Cancer Biology 2022Quote: ... Fractions 2–9 were diluted 1:10 in PBS and centrifuged at 20,000 ×g (bEVs) or 100,000 ×g (sEVs) at 4°C for 1 h with an MLS-55 rotor (Beckman Coulter). The bEV and sEV pellets were suspended in double 0.22-µm-filtered PBS and were either used directly for experiments or stored in aliquots at −80°C until subsequent BLI ...
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bioRxiv - Cell Biology 2023Quote: ... then incubated for 1 hr at RT before centrifugation at 100,000 x g (23°C) in a TLA100 rotor (Beckman-Coulter). The top 50% of the supernatant was removed ...
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bioRxiv - Developmental Biology 2023Quote: ... Libraries were purified using a ratio of 1:0.85 sample to bead ratio using Agencourt AMPure XP SPRI beads (Beckman Coulter). The final products were quantified on a D5000 Tapestation (Agilent ...
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bioRxiv - Neuroscience 2023Quote: ... Homogenized tissue was sonicated 20 times with 1 sec pulses and then centrifuged using a SW55 Ti rotor (Beckman Coulter) at 42,700 rpm for 15 min at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... The lysate was separated into soluble and insoluble fractions by ultracentrifugation at 100,000 x g for 1 h using Optima L-XP (Beckman Coulter). The soluble fraction was filtered using a bottle-top 0.22-µm filter (Corning) ...
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bioRxiv - Neuroscience 2023Quote: Monomeric wild-type α-syn was ultracentrifuged at 90,000 RCF for 1 hour at 4 °C to remove any amorphous aggregates (Beckman Coulter Optima Max-XP with MLA-130 rotor) ...
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Dopaminoceptive D1 and D2 neurons in ventral hippocampus arbitrate approach and avoidance in anxietybioRxiv - Neuroscience 2023Quote: ... 10 mM Tris-HCl) and centrifugated at 107,000 g for 1 hour at 4°C in a SW41Ti Swinging-Bucket Rotor (Beckman Coulter). Supernatant was discarded and nuclei pellets were re-suspended in 800 µL of PBS supplemented with 0.5% bovine serum albumin (BSA) ...
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bioRxiv - Plant Biology 2023Quote: ... It was then centrifuged again at 100,000 x g for 1 h at 4 ⁰C (TLA 55 rotor, Beckman Coulter; OptimaTM Max ultracentrifuge, Beckman Coulter). The final pellet containing the purified EV fraction was resuspended in 50 µl of 20 mM Tris-HCl pH 6.0 ...
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bioRxiv - Plant Biology 2023Quote: ... at 10,000 x g for 1 h at 4 ⁰C (JA-17 rotor, Beckman Coulter; Avanti J-20TM centrifuge, Beckman Coulter). The supernatant from this step was decanted into the new tubes and centrifuged at 39,800 x g for 2 h at 4 ⁰C (JA-17 rotor ...
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bioRxiv - Cell Biology 2023Quote: ... loaded onto a discontinuous 0.75/1.0/2.33 M sucrose gradient and spin at 242,000 g for 1 h at 4 °C in a SW32Ti rotor (Beckman & Coutler). Growth cone fractions were collected at the interface load/0.75 M sucrose and non-growth cone fractions collected between 0.75/1.0 M sucrose and then analyzed by Western Blot.