Labshake search
Citations for Beckman :
651 - 700 of 1820 citations for 1 6 Chloropyridazin 3 yl piperidine 4 carboxamide since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... Cells were harvested by two rounds of centrifugation (6,000 rpm, 10 min, 4°C, JLA-9.1000 rotor; Beckman), resuspended in Buffer A containing 50 mM Tris-HCl pH8.0 ...
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bioRxiv - Neuroscience 2024Quote: ... the samples were ultracentrifuged (4 °C for 60 min) at 100,000×g (Optima MAX Preparative Ultracentrifuge, Beckman Coulter). Supernatants ...
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bioRxiv - Cancer Biology 2023Quote: ... the high-speed centrifugation at 167,000g for 4 hours in an SW32 Ti swinging-bucket rotor (Beckman Coulter) was used to separate and further wash the EV from the concentrate collected in the previous step ...
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Faa1 membrane binding drives positive feedback in autophagosome biogenesis via fatty acid activationbioRxiv - Biochemistry 2023Quote: ... Lysates were cleared by centrifugation (20,000 rpm for 45 min at 4°C in a Beckman SW45Ti rotor). The supernatant was incubated with IgG Sepharose beads (GE Healthcare ...
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bioRxiv - Microbiology 2024Quote: ... The eluent was ultracentrifuged (100,000 ×g, 4°C, 16 h) using Optima L-100 XP Ultracentrifuge (Beckman Coulter) to sediment ribosomes.
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bioRxiv - Microbiology 2022Quote: ... The supernatant fractions were collected and centrifuged at 75,600 x g for 90 min at 4 °C (Beckman JA25.50 rotor ...
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bioRxiv - Molecular Biology 2022Quote: ... The elutions were combined and centrifuged at 186,000 x g at 4 °C for 1.5 h in a (TLA-120.1) rotor (Beckman Coulter). Supernatant was discarded and the pellet was resuspended in the RNC buffer at a concentration of ~200 nM ...
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bioRxiv - Biochemistry 2022Quote: ... Membrane contamination was removed by centrifugation 100,000 x g 1.25 hours 4°C in an NVT65.2 rotor (Beckman Coulter). The clarified supernatant was concentrated to 50 µl by ultrafiltration (Sartorius Vivaspin Turbo ...
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Dynamic states of eIF6 and SDS variants modulate interactions with uL14 of the 60S ribosomal subunitbioRxiv - Biochemistry 2022Quote: ... Gradients were centrifuged at 49,100g for 16 to 17 hrs at 4°C in an SW32Ti rotor (Beckman). Gradients were fractionated using a Brandel (UV ...
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bioRxiv - Molecular Biology 2022Quote: ... and spun at 100,000g for 70 minutes at 4°C (Optima XPN-80 Ultracentrifuge, Beckman Coulter, California, USA) in a 45ti fixed angle rotor ...
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bioRxiv - Neuroscience 2022Quote: ... were then spun at 19,000rpm/90 minutes/4°C using an ultracentrifuge and a SW28 hanging rota (Beckman). All supernatant was removed and each viral pellet was resuspended with 300 µl of 1% bovine serum albumin (Tocris Bioscience ...
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bioRxiv - Genomics 2023Quote: ... Extracts were then centrifuged at 148,000 g for 2.5 hours at 4°C using the SW 28 Ti rotor (Beckman). Resulting pellet was resuspended overnight at 4°C in 1.5 ml of HBSS ...
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bioRxiv - Bioengineering 2023Quote: ... at 4°C using an Optima Max Ultracentrifuge and a TLA-55 rotor (Beckman Coulter, Brea, CA, USA). The pellet from this supernatant (i.e. ...
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bioRxiv - Cell Biology 2023Quote: ... Mitochondrial membranes were pelleted at 72000 xg for 30 min at 4°C using an optimaTM ultracentrifuge (Beckman) with Beckman rotor (TLA 120) ...
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bioRxiv - Biophysics 2024Quote: ... Lysates were then clarified by centrifugation (100,000 xg, 60 min, 4 °C, JA 30.50 rotor, Avanti JXN-26 centrifuge (Beckman)) before filtering the supernatant through 0.45 μm syringe filters ...
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bioRxiv - Microbiology 2024Quote: ... The cells were collected by centrifugation (8,000 rpm, 10 minutes at 4°C, JLA-10.500 rotor (Beckman Coulter)) ...
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bioRxiv - Cell Biology 2024Quote: ... Following an 18-hr centrifugation at 4°C (44,000 rpm, SW55 rotor, Beckman Optima LE-80 K Ultracentrifuge), 420-μl fractions (1-11 ...
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bioRxiv - Neuroscience 2024Quote: ... Ultracentrifugation was done at 134,000xg for 4h at 4°C using an SW 41 Ti rotor (Beckman Coulter) in an Optima XE-90 ultracentrifuge (Beckman Coulter) ...
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bioRxiv - Biochemistry 2024Quote: ... microsomes from the IVT reactions were first recovered by centrifugation at 4°C in the TLA55 rotor (Beckman) for 20 min at 55,000 rpm ...
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bioRxiv - Immunology 2024Quote: ... and 20% OptiPrep density gradient at 100,000 × g overnight and at 4 °C (SW 40 Ti, Beckman Coulter). The following day ...
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bioRxiv - Microbiology 2024Quote: ... cultures were harvested by centrifugation for 30 minutes at 5,000 rpm and 4 °C in an Avanti JXN-26 Floor Centrifuge using the JXN 12.500 rotor (Beckman). The resulting pellets were resuspended in 40 mL Lysis buffer (20 mM HEPES pH 7.5 ...
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bioRxiv - Cell Biology 2024Quote: ... The post-nuclear supernatants were centrifuged at 60,000 rpm for 60 min at 4°C with a TLA 120.2 rotor (Beckman) to collect ER-mitochondria-enriched fractions ...
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bioRxiv - Genomics 2024Quote: ... by centrifuging the chip facing downwards at 3,220 g for 5 min at 4 °C and purified with 1.5 × Ampure XP beads (Beckman), followed by DNA QC and trace checking.
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bioRxiv - Biochemistry 2024Quote: ... and subjected to centrifugation at 35,000 rpm for 2.5 hr at 4 °C using a SW41Ti rotor (Beckman). The polysome profile in sucrose gradients was resolved using a Brandel gradient fractionator ...
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bioRxiv - Cancer Biology 2024Quote: ... followed by ultracentrifugation at 100,000 g for 90 min at 4°C (45Ti or TLA110 rotors, Beckman Coulter). The pellet containing EVs was washed in cold 1x PBS (Gibco ...
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bioRxiv - Biophysics 2024Quote: ... and the lysate was cleared by ultracentrifugation for 30 minutes at 4°C (Ti45 rotor, 37,000rpm, Beckman Coulter). The proteins were purified by affinity chromatography using an ÄKTA Start (GE Healthcare ÄKTA Start) ...
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bioRxiv - Microbiology 2021Quote: ... PE) and HIV-1 Gag core antigen (FH190-1-1, Beckman Coulter; PE, FITC). All samples were acquired on either an BD Fortessa X20 or LSR II using BD FACSDiva software and analyzed using FlowJo v10 (Tree Star) ...
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bioRxiv - Immunology 2024Quote: ... PD-1 (PE-Cy5.5, 1/320, Beckman Coulter). Staining was performed in 1X PBS with 5% FBS and incubated in the dark for 30 minutes ...
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bioRxiv - Immunology 2024Quote: ... PD-1 (PE-Cy5.5, 1/320, Beckman Coulter). Staining was performed in 1X PBS with 5% FBS and incubated in the dark for 30 minutes ...
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bioRxiv - Genetics 2020Quote: ... PCR products were pooled by region of interest using 2-3 μL of each reaction and the resulting pools of products were purified using a 1X Ampure XP cleanup (Beckman Coulter). Purified libraries were pooled and submitted for paired-end 150 cycle sequencing on an Illumina MiSeq ...
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bioRxiv - Genomics 2021Quote: ... Cells were washed with 25 mm supplemented HBSS before depleting the sample of unnucleated cells using IOTest 3 Lysing solution (Beckman Coulter) following provider instructions ...
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bioRxiv - Microbiology 2021Quote: ... triplicate spore preparations were made in 3 ml of 20% glycerol and suspended in Isoton II (Beckman Coulter, Brea, CA, USA). The particle volume was then measured for a minimum of 100 000 particles per sample using a Multisizer 4e Coulter counter (Beckman Coulter ...
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bioRxiv - Cell Biology 2020Quote: ... with densities ranging from 1.10 to 1.15 were pooled and washed in PBS (Beckman Ti50, 3 h at 42 000 rpm).
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bioRxiv - Microbiology 2022Quote: A 3 ml volume of pseudoparticles was pelleted using a TLA-55 rotor with an Optima-MAX-E ultracentrifuge (Beckman Coulter) for 2 hours at 42,000 rpm at 4°C ...
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bioRxiv - Cancer Biology 2022Quote: ... 10 nM leptomycin b, vehicle control or library compounds (Supplementary Tables 2, 3) were added using an Echo liquid handler (Labcyte/Beckman Coulter). Cells were incubated for a further 24 h then fixed in 4% paraformaldehyde and stained with DAPI and Alexa Fluor 488 Phalloidin (ThermoFisher) ...
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bioRxiv - Cell Biology 2020Quote: ... 3 ml of 50% and 3 ml of 40% sucrose) in Ultra-Clear Beckman tubes and centrifuged at 26000 rpm (SW32Ti rotor, Beckman Coulter) for 1 h at 4°C ...
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bioRxiv - Biochemistry 2020Quote: ... and pelleted for 3 min in a swing-out rotor at 1000 rpm in a tabletop centrifuge (Allegra X-30R, Beckman Coulter). The supernatant was aspirated and cells were suspended in the remaining liquid to 1 × 105 – 1 × 107 cells/100 μl ...
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bioRxiv - Genetics 2020Quote: ... Timepoints were measured every 2-3 days by flow cytometry analysis of >7,000 cells (either a BD Accuri C6 or Beckman Coulter CytoFLEX). Data was analyzed using Cytoflow (https://github.com/bpteague/cytoflow ...
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bioRxiv - Genomics 2020Quote: ... We carried out the first round of PCR for 3 cycles and purified the reactions with Ampure XP beads (Beckman-Coulter). We then carried out a second round of PCR with primers targeting the P5 and P7 sequences only ...
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bioRxiv - Genomics 2021Quote: ... samples were purified with 45 µl of Agencourt AMPure XP magnetic beads with a sample to beads ratio of 3:2 (Beckman Coulter), normalized by Qubit quantification and pooled to generate a 4 nM library ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were harvested after 3 days and counted using a Z2 Coulter Particle Count and Size Analyzer (Beckman Coulter, Brea, CA).
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bioRxiv - Cancer Biology 2024Quote: ... The percentage of fluorescence-positive cells was determined initially (baseline reference, passage 0, 3 days after transduction) and all following passages using the CytoFLEX Flow Cytometer (Beckman Coulter). In each passage ...
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bioRxiv - Cell Biology 2023Quote: ... 30% Percoll (v/v)] followed by 3 mL of MRB in a 13 mL ultracentrifuge tube (Beckman Instruments, Inc, CA 331372) and spun at 95,000 x g for 45 min in a TH641 rotor ...
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bioRxiv - Genetics 2023Quote: ... the cell pellet was resuspended in PBS supplemented with 2 % FCS and nucleated cells were counted in methylene blue with 3 % acetic acid on a Vi-Cell XR cell viability counter (Beckman Coulter). 10 × 106 bone marrow cells were resuspended in 200 μl of PBS supplemented with 2 % FCS containing the following antibody solution ...
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bioRxiv - Molecular Biology 2024Quote: ... bottom layer was made by diluting OptiPrep™ solution with clarified HM or digesta sample (2.25 mL OptiPrep™ + 0.75 mL sample = 3 mL) into a 5 mL open-top thin wall ultra-clear tube (Beckman Coulter). A discontinuous gradient was made with 40% (w/v) ...
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bioRxiv - Bioengineering 2022Quote: ... Supernatant (33 mL) was placed on a 3 mL 25% (w/v) sucrose pad in SW28 tube (Beckman, Brea, CA, USA) and centrifuged at 24,000 rpm ...
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bioRxiv - Cancer Biology 2023Quote: Counting of CB CD34+ cells after 3 days of ex vivo co-culture was accurately obtained with flow-count fluorosphere beads (Beckman Coulter), in combination with the following antibodies ...
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bioRxiv - Immunology 2024Quote: ... Fluorescence signals and TruCount beads were acquired on a 3-laser CytoFLEX using Cytexpert software 2.3 (Beckman Coulter, Brea, CA, USA). Instrument quality control and standardization were performed daily using CytoFLEX Daily QC Fluorospheres (Beckman Coulter #B53230).
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bioRxiv - Pathology 2024Quote: ... with primers located in the U3 region of the 5’ and 3’ LTRs (Figure S1) and purified using “1X AmpureXP beads” (Beckman Coulter). ONT (Oxford nanopore technologies ...
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bioRxiv - Cancer Biology 2024Quote: ... washed 3 times with chilled phosphate-buffered saline (PBS) and analyzed by flow cytometry (CytoFLex, Beckman Coulter Inc., Brea, CA, USA)[39 ...