Labshake search
Citations for Beckman :
601 - 650 of 969 citations for Rat Hypoxanthine Phosphoribosyltransferase 1 HPRT1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2022Quote: ... We cleaned the reaction using 10 μl (1 x ratio) AMPure XP beads (Beckman Coulter) and eluted the reaction in 20 μl UltraPure DNase/RNase free ...
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bioRxiv - Cancer Biology 2023Quote: ... 25mM HEPES (pH 7.0) and 1% FBS and sorted with the Astrios EQ (Beckman Coulter) into RPMI1640 (Corning ...
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bioRxiv - Molecular Biology 2023Quote: Sedimentation velocity (SV) experiments were conducted using an Optima XL-1 analytical ultracentrifuge (Beckman Coulter). Samples were loaded into standard double-sector cells ...
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bioRxiv - Neuroscience 2024Quote: ... the cell lysate underwent centrifugation at 12,000 × g for 1 h (JA 25.50, Beckman Coulter) and supernatant was loaded to anti-Flag M2 affinity resin (GenScript) ...
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bioRxiv - Molecular Biology 2020Quote: ... entire ChIPed DNA or 300ng of the input DNA were used to construct libraries using NEBNext® Ultra™ II DNA Library Prep kit with AMPure XP magnetic beads (Beckman Coulter). Library quality and quantity were estimated with Bioanalyzer and Qubit assays ...
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bioRxiv - Cancer Biology 2020Quote: ... DNA was extracted from fresh frozen tissue or cell lines using the Agencourt DNAdvance Beckman Coulter kit (#A48705, Beckman Coulter, Brea, CA, USA), fragmented and submitted to a series of enzymatic steps including End-repair and Adenylation ...
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bioRxiv - Microbiology 2022Quote: ... Libraries were checked on agarose gel for successful amplification and purified with an Agencourt AMPure XP kit (Beckman Coulter Inc., Brea, CA, USA) using the supplier’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... were collected from microplates and washed twice in PBS and then resuspended in 50 μl of detergent (DNA-Prep Reagent Kit; Beckman Coulter, Brea, CA) containing 10 ng/ml propidium iodide (DNA-Prep Reagent Kit ...
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bioRxiv - Genomics 2024Quote: ... All DNA samples were cleaned three times using Agencourt AMPure beads prior to starting the 1D library kit (Beckman Coulter, Inc., CA, USA). Genomes were assembled with Canu v1.6 with a minimum read length of 5 kbp (45) ...
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bioRxiv - Microbiology 2023Quote: ... The PCR products were then pooled for each of the 16S rRNA and fungal ITS1 regions after a purification/equalization process with the AMPureXP Kit (Beckman Coulter, Inc., Brea). Primer dimers ...
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bioRxiv - Immunology 2023Quote: ... GEMs were broken and complementary DNA (cDNA) was cleaned up with DynaBeads MyOne Silane Beads (10x Genomics, No. 2000048) and SPRIselect Reagent Kit (Beckman Coulter, No. B23318). Full-length ...
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bioRxiv - Cell Biology 2020Quote: ... and then the sample was centrifuged at 182,000g for 1 h at 4°C (Beckman SW40). LD fraction was carefully collected from the top band of the gradient and washed three times using 200 μL of buffer B ...
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bioRxiv - Immunology 2021Quote: ... The lysate was centrifuged at 42,000 RPM for 1 h (45 Ti fixed-angle rotor, Beckman), and the supernatant was used for affinity chromatography with MBP beads by gravity flow ...
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bioRxiv - Immunology 2021Quote: ... The lysate was centrifuged at 42,000 RPM for 1 h (45 Ti fixed-angle rotor, Beckman), and the supernatant was used for affinity chromatography with anti-FLAG M2 affinity gel (Sigma ...
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bioRxiv - Evolutionary Biology 2021Quote: ... and diluted 1:210 into 8 new wells using a Biomek-FX pipetting robot (Beckman-Coulter). This split-pool strategy was designed to mimic the evolution conditions while maintaining sufficient diversity for bulk fitness measurements ...
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bioRxiv - Genomics 2020Quote: ... amplicons were purified using 1 x Agencourt AMPure XP beads (Beckman Coulter Life Sciences, United States), eluted in 20 μl of 10 mM Tris (pH 8.5) ...
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bioRxiv - Neuroscience 2019Quote: ... and 3% Percoll (1 ml each) in Polycarbonate centrifuge tubes (13 x 51 mm; Beckman Coulter). The gradients were then centrifuged for 15 min at 18,700 rcf ...
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bioRxiv - Developmental Biology 2020Quote: ... and tagmented DNA was purified with a 1.5:1 ratio of AMPure XP beads (Beckman, A63880) following the manufacturer’s instructions ...
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bioRxiv - Biophysics 2020Quote: ... and 0.5 mM EDTA) supplemented with 1 mM DTT at 125,000 × g (SW Ti40 rotor, Beckman) for 16 hours at 4 °C ...
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bioRxiv - Cell Biology 2020Quote: ... Lysate was clarified by centrifugation at 55,000 g for 1 h in 25.50 rotor (Beckman Coulter). Clarified supernatant was loaded on 1.5 mL of amylose resin (NEB ...
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bioRxiv - Cell Biology 2020Quote: ... This product was then purified and concentrated to >1 μM using magnetic SPRI beads (Beckman Coulter). 2.2 μl Cas9 buffer (580 mM KCl ...
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bioRxiv - Microbiology 2020Quote: ... DNA was purified twice using 1× volume of AMPure XP beads (Beckman Coulter, Cat. No. A63882) with slight modifications to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2021Quote: ... using 1-10 ng DNA and libraries were size selected with AMPure XP beads (Beckman Coulter). Illumina 50 bp single-end read sequencing was performed on a NextSeq 500 or NovaSeq 6000 ...
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bioRxiv - Bioengineering 2021Quote: ... cells were washed with 1% BSA in DPBS and analyzed using CytoFLEX LX (Beckman Coulter, USA). Isotype controls served for negative gating.
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bioRxiv - Molecular Biology 2021Quote: ... cells were permeabilized and intracellular staining of RD1-conjugated antiHIV-1 p24 (Beckman Coulter, Brea, CA). Flow cytometry was performed using BD Fortessa II instrument (BD Biosciences ...
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bioRxiv - Physiology 2022Quote: Tritium from [1-3H]-glucose enrichment was detected by liquid scintillation spectrometry (Beckman Coulter LS 6500).
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bioRxiv - Cell Biology 2022Quote: ... and incubated for 1 hr at +4°C before the flow cytometry analysis (Beckman Coulter CytoFlex) or sorting (SONY MA900) ...
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bioRxiv - Microbiology 2021Quote: ... 1 mM EDTA) and cesium chloride (7.163 M) in 3.3 ml polyallomer centrifuge tubes (Beckman Coulter) to a final density of 1.700 g ml-1 ...
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bioRxiv - Microbiology 2022Quote: ... Cell debris was removed by centrifugation (25,000 rpm for 1 hour, JA-25.50 rotor (Beckman Coulter)) ...
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bioRxiv - Synthetic Biology 2020Quote: ... Samples were then purified using 1 volume of Agencourt AMPure XP beads (Beckman Coulter, cat. A63880) and used as input for library preparation using NEBNext Ultra II DNA Library Prep Kit (New England Biolabs ...
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bioRxiv - Microbiology 2019Quote: ... and at least 1 × 104 spores were examined by FC500 MPL flow cytometer (Beckman Coulter, USA). Expression of the CotB-HcGAPDH fusion protein was analyzed using FlowJo software (Tree Star ...
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bioRxiv - Immunology 2020Quote: ... were incubated with 1-2 million 1D8 cells following standard FACS protocol and analyzed by Beckman.
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bioRxiv - Immunology 2021Quote: ... VLPs were concentrated using ultracentrifuge (TLA100.3 rotor, Beckman; at 72,000 rpm for 1 hour, +5°C) or by ultrafiltration using Amicon Ultra 15 (100 kDa ...
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bioRxiv - Molecular Biology 2022Quote: ... for 1 additional hour at 55°C and purified using 2x AMPure XP beads (Beckman Coulter). The generated libraries were amplified using the KAPA HiFi Hotstart Ready Mix and Nextera Index Kit 1 (i7 ...
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bioRxiv - Microbiology 2023Quote: ... Three 1:2 serial dilutions were done suing a Biomek i7 liquid handler (Beckman Coulter, USA), in order to obtain the four working concentrations ...
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bioRxiv - Immunology 2023Quote: ... manual gating analysis (Supplementary figure 1) was performed with Kaluza V2.1 software (Beckman coulter, IN, USA).
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bioRxiv - Cell Biology 2022Quote: ... The PCR primers were removed with 1x 0.9:1 SPRI beads (Beckman Coulter, Cat no. A63880) according to manufacturer’s instructions and samples eluted in 20μl ...
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bioRxiv - Biochemistry 2023Quote: ... The crude lysate was centrifuged for 1 hour at 20,000 rpm and 4 °C (Beckman Coulter). The supernatant was filtered using a 0.44 μm syringe filter (Sartorius ...
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bioRxiv - Molecular Biology 2023Quote: ... A second cleanup of the libraries was performed using 1 volume of AMPure XB beads (Beckman). Libraries were eluted in 20 µl of nuclease-free H2O (Ambion) ...
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bioRxiv - Biophysics 2023Quote: ... The cell lysate was centrifuged at 4 °C at 35000 rpm for 1 hour by Beckman Optima™ L-80XP ultracentrifuge equipped with a Ti-45 rotor (Beckman ...
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bioRxiv - Microbiology 2023Quote: ... Diluted samples (1 in 100 in PBS) were analysed on a Cytoflex flow cytometer (Beckman Coulter), to count GFP-PA01 cells (ex ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... 18 ◦C for 1 h 45 min in a 50.2Ti rotor (Beckman-Coulter, Brea, CA, USA). After ultracentrifugation ...
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bioRxiv - Biochemistry 2024Quote: Bacteria were collected in 1 L buckets by centrifugation at 5000 rpm (JLA8.1 rotor, Beckman-Coulter) for 20 min ...
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bioRxiv - Biochemistry 2024Quote: ... Bacteria were collected in 1-L buckets by centrifugation at 5000 rpm (JLA8.1 rotor, Beckman-Coulter) for 20 minutes ...
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bioRxiv - Evolutionary Biology 2021Quote: ... We prepared sequencing libraries using the New England Biolabs Ultra II kit with the only modification being the use of Ampure XP beads for cleanup and size selection (Beckman Coulter, Brea, CA, USA). Following adapter ligation a single size selection was conducted at 0.65X ...
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bioRxiv - Evolutionary Biology 2021Quote: ... one half was purified using silica columns (MinElute PCR Purification Kit) and the other using 3x SPRI beads (Beckman Coulter™ Agencourt AMPure XP). Indexing PCR ...
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bioRxiv - Cancer Biology 2022Quote: Genomic DNA was isolated from all TP53-KO minor cells treated in F10 matrigel assays using the Agencourt DNAdvance Genomic DNA Isolation Kit (Beckman Coulter, Cat. No. A48705), and the TP53 locus was amplified using the following primers ...
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bioRxiv - Biochemistry 2020Quote: ... Cell lysates were collected and RNA was isolated using the Biomek FxP and the RNAdvance Tissue Isolation kit (Beckman Coulter, USA, p/n A32646). The resulting RNA was quantified using the Nandrop 8000 (Nanodrop ...
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bioRxiv - Microbiology 2022Quote: ... The success of the amplifications was checked in a 2% agarose gel and PCR products were subsequently purified using Agencourt AMPure XP magnetic beads kit (Beckman Coulter, Brea, CA, USA), quantified with the QuantiFluor™ dsDNA System (Promega ...
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bioRxiv - Microbiology 2019Quote: ... 4th and 8th week using assay kits (BioSino Bio-Technology & Science Inc., Beijing, China) and the AU480 Chemistry analyzer (Beckman Coulter Inc., California, USA).