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Citations for Beckman :
601 - 650 of 684 citations for Puumala Virus Glycoprotein 1 Gn Human Fc tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... the 4 tubes were centrifuged using JA-20 rotor at 43,000 × g for 1 hour and 30 minutes in Beckman coulter® Avanti j-26 xp (Beckman, France). The pellets of the 4 tubes were preserved at −80°C before transport and metagenome sequencing and analysis.
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bioRxiv - Immunology 2021Quote: ... The PBMC were resuspended in 1 mL cold PBS+BSA per 15 mL whole blood processed and counted with a ViCell (Beckman Coulter) using VersaLyse reagent (Beckman Coulter #A09777 ...
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bioRxiv - Immunology 2021Quote: ... 0.1 ng cDNA was used per sample and the libraries were cleaned up with 1.8x AMPure® XP beads (Beckman Coulter). Quality and integrity of nucleic acids were assessed using the Agilent Technologies 2100 Bioanalyzer (Agilent Technologies ...
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bioRxiv - Immunology 2021Quote: ... either 150 μl or a maximum of 1 × 106 viable lymphocytes were analyzed using a CytoFLEX LX Flow Cytometer (Beckman Coulter), and data was analyzed with FlowJo software (FlowJo LCC ...
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bioRxiv - Genetics 2019Quote: ... The PCR products were examined on either 3% agarose gel by electrophoresis at 130 V for 1 hour or using capillary electrophoresis on an automated CEQ2000 system (Beckman-Coulter) as described previously (Raman et al. ...
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bioRxiv - Biochemistry 2022Quote: ... to remove intact cells and the supernatant was centrifuged again at 25,000 RCF for 1 hour (Beckman Coulter JA-20 rotor). The pellet was resuspended in lysis buffer and sodium cholate was added to a final concentration of 1% and stirred at 4°C for 1 hour ...
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bioRxiv - Microbiology 2023Quote: ... and permeabilized in PBS/1% BSA/0.1% saponin before staining with a FITC-conjugated anti-Cap24 (KC57-FITC, Beckman Coulter, France) for 1h at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... 1 μl or 10 μL of dilution were added to the 384-well plates using automated dispenser BioMek FX (Beckman Coulter). Extracellular bacteria were then eliminated by adding antibiotic to the media 1h after infection ...
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bioRxiv - Synthetic Biology 2023Quote: ... Droplets were made by either manually pipetting 0.05-0.1 μL droplets or dispensing individual 2.5 nL - 20 nL droplets using an Echo 550 acoustic liquid handler (Beckman Coulter, USA) into 2 μL of the lipid-oil phase in 384-well microplate ...
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bioRxiv - Cell Biology 2023Quote: ... Homogenates were then transferred to a 50 ml tube and centrifuged for 10 mins at 1 000 × g at 4°C (Beckman Coulter). The resulting supernatant was then transferred to microfuge tubes for storage at -80°C as whole liver lysates or to fresh ultracentrifuge tubes for further processing.
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bioRxiv - Biochemistry 2023Quote: ... to remove intact cells and the supernatant was centrifuged again at 25,000 RCF for 1 hour (Beckman Coulter JA-20 rotor). The pellet was resuspended in lysis buffer and sodium cholate was added to a final concentration of 1% and stirred at 4°C for 1 hour ...
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bioRxiv - Immunology 2023Quote: ... cells were washed with 1% FBS in PBS antibody staining buffer and stained with the following antibodies: CD64 PE-Cy7 (Cat. # B06025, Beckman Coulter); CD45 PerCP (Cat ...
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bioRxiv - Bioengineering 2023Quote: ... The suspension was then ultracentrifuged (58,000 × g, 1 hr., 4 °C) in an Optima MAX-XP (Beckman Coulter, Indianapolis, IN, USA) to separate residual catalyst ...
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bioRxiv - Biochemistry 2023Quote: GFP fluorescence level (FITC channel, signal amplification = 1) and cell size (FSC channel, signal amplification = 200) were measured with flow cytometer CytoFlex S (Beckman Counter) and CytExpert Software in 10000 cells (per each sample ...
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bioRxiv - Genomics 2023Quote: PCR reaction products were cleaned and size selected using a 0.7:1 ratio of AMPure XP SPRI beads (Beckman Coulter Inc.) to sample ...
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bioRxiv - Plant Biology 2024Quote: ... Then the reaction was started by adding 150 µL of a 1 M proline solution and the decrease in the OD600 monitored until at least 1 min of linear reaction was observed (DU-800 Spectrophotometer, Beckman Coulter). Reaction rates were calculated using the 600 nm absorbance coefficient of DCPIP (19.1 mM-1 cm-1 ...
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bioRxiv - Microbiology 2024Quote: ... The final Hcp1-VLPs particles were pelletized by ultracentrifugation at 274,000 ×g at 4°C for 1 h in a SW41 Ti rotor (Beckman Coulter, Inc). The final pellet was resuspended in PBS pH 7.4.
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bioRxiv - Microbiology 2024Quote: Intracellular HIV-1 p24 staining was performed on fixed U1 and ACH-2 cells with KC57-FITC (Beckman Coulter, Cat#6604665). After allowing at least 30 minutes for fixation ...
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bioRxiv - Cell Biology 2024Quote: ... plasma samples were thawed on ice and subjected to ultracentrifugation at 100,000 g for 1 hr or 18 hr at 4 °C (TLA-55 rotor; Optima MAX-MP Tabletop Ultracentrifuge, Beckman Coulter). Pellets were washed once in 1.0 ml PBS ...
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bioRxiv - Neuroscience 2023Quote: ... The absorbance was recorded at 1 min intervals for 10 min at 340 nm at 30°C using a spectrophotometer (DU 800, Beckman Coulter). The enzymatic activity was determined as the change in absorbance/min/mg protein.
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bioRxiv - Cancer Biology 2024Quote: ... 200 μl of the PCR reaction (roughly corresponding to 1 μg of amplicon) was then purified using a double-sided selection with AMpure XP beads (Beckman Coulter). First ...
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bioRxiv - Biophysics 2020Quote: ... The lysates were clarified by centrifugation (16,000 rpm for 1 h at 4°C, rotor JA 25.50, Beckman Coulter, Brea, California, USA). The supernatant was incubated with amylose resin for 1 h at 4°C and loaded onto a 20 ml chromatography column (Bio-Rad ...
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bioRxiv - Biophysics 2020Quote: ... Very small amounts of fibrillar species formed during this time were removed by ultracentrifugation for 1 h at 90000 rpm (Optima TLX Ultracentrifuge, Beckman Coulter, TLA-120.2 Beckman rotor, 288000 g). The excess monomeric protein and some small oligomers were then removed by multiple filtration steps using 100-kDa cut-off membranes ...
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bioRxiv - Genetics 2021Quote: ... A final DNase treatment was performed in 50 μL total volume using 1 μL of Turbo DNase with addition of the buffer incubated for 60 min at 37 °C followed by inactivation with 1 μL of 10% SDS and purification using RNA clean SPRI beads (Beckman Coulter, A63987).
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bioRxiv - Microbiology 2019Quote: ... Cells were permeabilized in 0.5% Triton-X for 10 min and stained with 1:300 KC57-FITC (Beckman Coulter 6604665; RRID: AB_1575987). Data were collected on Accuri C6 (BD Biosciences – U Mass ...
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bioRxiv - Systems Biology 2020Quote: ... Next the cultures were diluted 1:40 with 0.5M NaCl and immediately measured in Multisizer™4 COULTER COUNTER® (Beckman Coulter) (Coulter ...
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bioRxiv - Molecular Biology 2021Quote: ... Resulting libraries were pooled and treated with AMPure XP magnetic beads at a ratio of 0.8:1 beads:sample volume to purify DNA (Beckman Coulter, Brea, CA, USA). Library yield and quality was determined by TapeStation electrophoresis with HSD1000 ScreenTapes (Agilent Technologies).
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bioRxiv - Immunology 2019Quote: ... SSM cultures were grown for ∼22 h at 36°C with shaking at 180 rpm until the OD600 reached 0.5 on a 1 cm path width spectrophotometer (Beckman Coulter DU 530). The cultures were then diluted to 1 × 109/ml with SSM.
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bioRxiv - Genomics 2019Quote: ... Cells were suspended with 300 μl 1% FBS /HBSS (with 10 mM sodium butyrate) and subjected to FACS (Beckman Coulter MoFlo XDP) sorting for tdTomato positive cells ...
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bioRxiv - Biochemistry 2020Quote: ... to a final volume of 1 ml and then further centrifuged (120000 g, 4°C, 2 h) in a TLA-120.2 rotor (Beckman Coulter, Brea, CA). Pellets were washed twice with 4°C cold PBS and centrifuged (120000 g ...
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bioRxiv - Biophysics 2020Quote: ... The lysate was then centrifuged at 4° C in the SW32Ti rotor with a speed of 30,000 rpm for 1 h to pellet the membrane fraction using an Optima L-90K Ultracentrifuge (Beckman Instruments, Brea, CA). The pellet was resuspended in HND buffer and bulk membrane proteins were extracted by adding 2% ANAPOE-X-100 (Anatrace ...
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Hydrogen sulfide blocks HIV rebound by maintaining mitochondrial bioenergetics and redox homeostasisbioRxiv - Microbiology 2021Quote: ... Permeabilized cells were then incubated with 50 μl of 1:100 dilution of phycoerythrin (PE)-conjugated mouse anti-p24 monoclonal antibody (KC57-RD1; Beckman Coulter, Inc.) for 30 min at room temperature ...
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Micro RNAs are minor constituents of extracellular vesicles and are hardly delivered to target cellsbioRxiv - Cell Biology 2020Quote: ... Cat no UZ-PA-38.5-1) at 100,000 g at 4 °C for 2 h in a swinging-bucket rotor (Beckman Coulter; SW28 or SW32).
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bioRxiv - Cell Biology 2020Quote: ... anti-CD105 and anti-HLA-ABC antibodies (suppl. Table 1) and analysed by flow cytometry (Cytoflex; Software Cytexpert 2.3, Beckman-Coulter, Krefeld, Germany). Upon passage 3 ...
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bioRxiv - Plant Biology 2020Quote: ... Double size selection was carried out by adding 50 μL of a 1:4 (0.5X) mixture of AMPure XP Beads (Beckman Coulter, Brea, USA) to PEG buffer (20% PEG w/v ...
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bioRxiv - Plant Biology 2020Quote: ... Soil pH and EC were 8.3 and 12 dS m−1 that were measured by a pH meter (Beckman 45 Modal, US) and EC meter (VWR Conductivity Meter DIG2052 ...
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bioRxiv - Microbiology 2019Quote: ... Permeabilized cells were then incubated with 100 μl of 1:100 dilution of phycoerythrin-conjugated mouse anti-p24 mAb (KC57-RD1; Beckman Coulter, Inc.) for 30 min at 4 °C with intermittent mixing ...
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bioRxiv - Microbiology 2020Quote: ... Membrane-containing fractions were collected in 1 mL after ultracentrifugation at 35,000 rpm on an SW-40 rotor (Beckman, Brea, CA USA) for 6 hours at 4°C and stored at -80°C prior to analysis.
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bioRxiv - Evolutionary Biology 2021Quote: ... with NEBNext Multiplex Oligos for Illumina® (Dual Index Primers Set 1) and AMPure XP magnetic beads (Beckman Coulter, Brea, CA, USA). DNA content and quality of indexed libraries were examined with a Qubit 4.0 using the high-sensitivity kit (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2019Quote: ... in order to achieve a starting density of 1.70 g mL-1 in a 3.3 mL polyallomer OptiSeal tubes (Beckman Coulter, Brea, CA, USA). After ultracentrifugation ...
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bioRxiv - Microbiology 2022Quote: ... Cells were permeabilized in 0.5% Triton-X for 10 min and stained with 1:300 KC57-FITC (Beckman Coulter 6604665; RRID: AB_1575987) or 1:300 KC57-RD1 (numbers) ...
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bioRxiv - Molecular Biology 2023Quote: ... The cell lysate was centrifuged at 4 °C at 35000 rpm for 1 hour with Beckman Optima™ L-80XP Ultracentrifuge equipped with Ti45 rotor (Beckman, USA). The pellet containing membranes and cell debris was discarded ...
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bioRxiv - Neuroscience 2022Quote: ... The loaded gradient was ultracentrifuged at 69,150 x g for 1 h at 4 °C (SW 41 Ti Swinging-Bucket Rotor, Beckman Coulter, Cat. #331362) and the synaptosome fraction was harvested from the interface between the two sucrose layers ...
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bioRxiv - Evolutionary Biology 2022Quote: ... and libraries were purified and size selected using Ampure XP Beads at a 1.8:1 bead volume:library volume (Beckman Coulter, Brea, CA, USA). Library quality and transposition efficiency was accessed using a Fragment Analyzer and PROSize 2.0 (Agilent ...
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bioRxiv - Microbiology 2023Quote: ... Cell pellets were permeabilized for intracellular staining via resuspension in 0.5% Triton X-100 in PBS for 10 min and were subsequently resuspended in a 1:500 dilution of anti-p24-FITC KC57 antibody (Beckman Coulter #6604665) in 1% BSA in PBS for 1 h at room temperature in the dark ...
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bioRxiv - Molecular Biology 2022Quote: ... The percentage of live/dead cells was determined within 1 hour of staining by flow cytometry (CytoFLEX, Beckman Coulter, Indianapolis, IN, USA). 10,000 events were collected per sample ...
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bioRxiv - Cell Biology 2023Quote: ... the supernatants were transferred into SW60 tubes (in vivo studies 1–4) or SW40 tubes (donated patients) (Beckman Coulter Inc., Brea, USA) and centrifuged at 10,000xg for 35 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... Supernatants were loaded onto a 1 M sucrose cushion and then pelleted by ultracentrifugation by using a TLA100.3 rotor (Beckman Coulter, cat#349490) for 1 hour at 100,000 rpm at 4°C ...
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bioRxiv - Plant Biology 2023Quote: ... DNA extracts were purified using a bead clean up method with a 2:1 ratio of Ampure XP beads (Beckman Coulter, USA) to DNA elution ...
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bioRxiv - Physiology 2023Quote: ... via centrifugation in a SW41 rotor at 42,000 g at 4 °C for 1 hour in a Beckman Coulter Optima L-100 XP ultracentrifuge using 14 × 89 mm Ultra-Clear Centrifuge Tubes (Beckman Instruments Inc.). Mitochondria were harvested at the interphase between 19 and 40% Percoll ...