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Citations for Beckman :
601 - 650 of 2048 citations for E 6 2 6 6 Trimethylcyclohex 2 en 1 yl hex 5 ene 2 4 dione since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... 4 °C (Beckman Coulter Optima LE-80K Ultracentrifuge). The clarified lysate was placed in a beaker on a stir plate at 4 °C and concentrated ammonium sulfate (5 mM HEPES pH 7.5 ...
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bioRxiv - Neuroscience 2020Quote: ... for 4 h with SW28 rotor (Beckman Coulter). The concentrated vector was stored at −80 °C until use ...
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bioRxiv - Biochemistry 2022Quote: ... 4 °C on a Sorvall WX90 ultracentrifuge (Beckman) with SW55Ti rotor ...
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bioRxiv - Molecular Biology 2022Quote: ... 4°C in SW-41Ti rotor (Beckman Coulter). Subsequently ...
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bioRxiv - Plant Biology 2021Quote: ... 4 °C for 18 h (Beckman SW41 rotor). The sugar solution contains 0.65 M sucrose ...
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bioRxiv - Biochemistry 2022Quote: ... 4°C using a SW41Ti rotor (Beckman Coulter). A BioComp fractionator was used to collect fractions 1 – 16 ...
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bioRxiv - Neuroscience 2022Quote: ... at 4°C in a Beckman ultracentrifuge (Beckman). After centrifugation ...
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bioRxiv - Systems Biology 2022Quote: A particle size analyzer (Multisizer 4, Beckman Coulter) was used to count the number of cells ...
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bioRxiv - Microbiology 2024Quote: ... 4 °C (Beckman Coulter, JA 25-50 rotor) for 10 min ...
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bioRxiv - Microbiology 2024Quote: ... 4°C (Beckman Coulter, JA 25-50 rotor) for 10 min and the cell lysates were incubated with 1 mL of prewashed Ni-NTA Agarose beads (QIAGEN ...
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bioRxiv - Biochemistry 2020Quote: ... The sample was centrifuged at 4°C for 1 hour and 40 minutes at 182,753 x g (38,000 rpm in a Beckman Coulter Type 42.2 Ti rotor).
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bioRxiv - Molecular Biology 2021Quote: ... 1 mM ATP and 1 mM PMSF) and centrifuged for 15 h at 4°C and 74 400 x g (Beckman SW 32.1 Ti). From the gradient ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... the cell lysate was layered on top of a discontinuous OptiPrep® gradient (50 %, 35 %, 30 %, 20 %, and 15 %) and centrifuged at 135,000 g for 1 h at 4°C (Beckman L8-80M Ultracentrifuge, USA). The melanin pellet was recovered ...
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bioRxiv - Microbiology 2024Quote: ... The 1 ml fraction containing the phage was then placed on a 40% (w/v) - 60% (w/v) sucrose gradient for 1 hour at 63,400 x g at 4°C (Beckman Coulter SW 40 Ti). The 1 ml fraction was then placed in a 50 kDa dialysis membrane (Spectrum Labs ...
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bioRxiv - Biochemistry 2024Quote: ... The A2aR-expressing membrane fraction was obtained by ultra- centrifugation at 100,000 g for 1 hour at 4°C in a Type 70 Ti Fixed-angle rotor (Optima XPN ultracentrifuge, Beckman Coulter Life Sciences). The pellet (membrane fraction ...
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bioRxiv - Immunology 2021Quote: ... were incubated with monoclonal antibodies at 4°C for 30 min and then fixed with 4% paraformaldehyde before analysis on a Cytoflex cytometer (Beckman Coulter). Data were analyzed by Flow Jo software v.10.7 (BD Biosciences) ...
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bioRxiv - Cell Biology 2021Quote: ... and loaded onto of a step-wise sucrose gradient as described by the manufacturer and subjected to ultracentrifugation at 150,000 xg for 4 h at 4 °C using SW50.1 rotor (Beckman Coulter, Mississauga, ON). Fractions were then collected and subject to denaturation with Laemmli buffer until further use.
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bioRxiv - Molecular Biology 2024Quote: ... They were cherrypicked from the original library plates and 4 crRNAs per target were pooled (4 x10 μl) and re-arrayed using a Biomek-FX automated pipetting system (Beckman Coulter). Edit-R CRISPR-Cas9 Synthetic tracrRNA (Horizon Discovery Biosciences ...
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bioRxiv - Microbiology 2024Quote: ... 21000 rpm for 5 h (Beckman SW41Ti). The band of the expected density was pooled using a syringe and the buffer was exchanged to the E-buffer using 100kDa VivaspinTM columns (Cytiva) ...
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bioRxiv - Systems Biology 2020Quote: ... and the resultant supernatant was ultracentrifuged at 100,000xg for 40 minutes at 4°C (rotor TLA 120.2; Beckman Coulter 343778 1 ml uncapped tubes).
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bioRxiv - Physiology 2021Quote: ... sEV were further isolated from lEV-depleted supernatants following a 100,000 xg ultracentrifugation step for 1 hour at 4°C (rotor MLA-50, Beckman Coulter Optima MAX-XP Ultracentrifuge) and two washes in NaCl before resuspension in NaCl.
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bioRxiv - Microbiology 2020Quote: ... The supernatant was filtered by 0.22 μm nylon membrane followed by centrifugation through a cushion of 50 % (w/v) sucrose with 36,000 rpm at 4 °C for 4 h (Beckman SW41 Ti rotor). The pellet was resuspended with PBS and the copy number of DWV was quantified by qRT-PCR.
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bioRxiv - Biophysics 2024Quote: ... The gradients were then centrifuged at 187,000 × g for 4 h at 4°C in a SW 41 Ti rotor (Beckman Coulter, Brea, CA). Bands corresponding to purified virus particles (∼1.36 g/mL ...
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bioRxiv - Microbiology 2020Quote: ... 4°C in SW 60 Ti rotor (Beckman Coulter) to pellet cyanophage particles ...
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bioRxiv - Immunology 2021Quote: ... CD38 APC-AF700 (clone LS198-4-3; Beckman Coulter), CD19 APC-Cy7 (clone SJ25C1 ...
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bioRxiv - Cell Biology 2020Quote: ... 4°C (SW32Ti rotor, Optima L-80XP ultracentrifuge, Beckman) with the maximum acceleration and minimum deceleration ...
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bioRxiv - Biochemistry 2021Quote: ... 4°C in swinging-bucket rotor SW40 (Beckman Coulter). Fractions (1 ml ...
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bioRxiv - Microbiology 2022Quote: ... at 4°C cells were resuspended in Isoflow (Beckman) for further reading the percentage of fluorescent labelled cells on the cytometer (FC500 ...
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bioRxiv - Microbiology 2022Quote: ... 4 °C (SW41 Ti Swinging-Bucket rotor, Beckman & Coulter). Bands containing virus particles were extracted with a syringe ...
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bioRxiv - Biochemistry 2023Quote: ... counted using a coulter counter (Beckman Coulter, Multisizer 4) and seeded onto 24-well dishes (Nunc ...
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bioRxiv - Cell Biology 2024Quote: ... at 4°C in a Ti70 rotor (Beckman Coulter). Clarified lysates were loaded using a sample pump or super-loop (Cytiva ...
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bioRxiv - Microbiology 2021Quote: ... and 1.3 mL of 5% (v/v) glycerol in TNE buffer) in 5 mL thin-walled Ultraclear centrifuge tubes (Beckman Coulter). Viral nucleocapsids were pelleted by centrifugation at 23,000 rpm in an SW 55 Ti rotor for 1 h at 4°C ...
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bioRxiv - Biophysics 2022Quote: ... Fractions of EV-specific density were then pooled together and subsequently concentrated via ultracentrifugation (100,000 × g, 4 h, 4 °C) through a 20% w/v sucrose cushion in a SW41 rotor (Beckman Coulter, Fullerton, CA, USA). The resulting supernatant was discarded ...
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bioRxiv - Neuroscience 2023Quote: ... PI (1 μl/100 μl) was added 5 minutes before starting the reading on the flow cytometer CytoFlex S (Beckman Coulter, Brea, CA, USA). Results were shown and analyzed through CytExpert flow cytometry analysis software.
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bioRxiv - Systems Biology 2020Quote: ... and 1 mL of 5% (w/v) iodixanol solutions on top of each other in a 5 mL open top polyallomer tube (Beckman Coulter). Crude exosomes were resuspended in 200 µL PBS and overlaid on top of the gradient ...
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bioRxiv - Genomics 2024Quote: ... and loaded onto 5 mL of neutral 5%–20% sucrose gradients prepared in TEN20 buffer in ultra-clear tubes (Beckman Coulter). Size separation of nucleic acids was done by ultra-centrifugation in SW50 rotor for 7 hours at 45 krpm at +4oC ...
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bioRxiv - Biophysics 2021Quote: ... centrifuged (Beckman Avanti, 1500 x g, 10 min, 4 °C) and pellets were flash-frozen and stored at −80 °C.
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bioRxiv - Immunology 2022Quote: ... for 90 minutes at 4°C (Optima XE-90, Beckman-Coulter with SW 32 Ti swinging bucket rotor ...
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bioRxiv - Molecular Biology 2022Quote: ... and ultracentrifuged (60,000 rpm, 4 °C, Beckman, 70 Ti rotor) for 20 hours ...
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bioRxiv - Synthetic Biology 2020Quote: ... After clarification (20,000 rpm, Beckman JA 25.5, 30min 4°C), lysate was incubated with Glutathione S-sepharose 4B resin (GE Healthcare ...
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bioRxiv - Biochemistry 2021Quote: ... Following centrifugation (Beckman SW40 rotor, 105,400xg, 18 h, 4°C), the heavier green band was collected and precipitated using 8% PEG6000 (Hampton Research ...
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bioRxiv - Immunology 2020Quote: ... for 90 minutes at 4°C (Optima XE-90, Beckman-Coulter with SW 32 Ti swinging bucket rotor ...
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bioRxiv - Cell Biology 2022Quote: ... at 4°C in a TLA-55 rotor (Beckman Coulter) at maximum speed (186000 g ...
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bioRxiv - Bioengineering 2023Quote: ... the centrifugation at 12,000 rpm 4 ℃ (Beckman Avanti J-26) for 30 mins was performed to remove cell debris ...
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bioRxiv - Biochemistry 2023Quote: ... 4 °C (Fixed angle Ti90 Rotor, Optima XE90, Beckman Coulter). The supernatant was loaded onto a 70 ml Q-sepharose column (XK 26/20 ...
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bioRxiv - Biophysics 2024Quote: ... 4 °C for 45 min in an ultracentrifuge (Beckman Coulter). The top fraction containing liposomes and the attached motors was harvested after the flotation assay and motor concentration was measured by GFP fluorescence.
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bioRxiv - Cell Biology 2022Quote: ... we used a CytoFLEX 5 flow cytometer (Beckman Coulter) and the CytExpert version 2.3 software ...
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bioRxiv - Immunology 2023Quote: ... KIR2DL1/S1 (Beckman Coulter, clone EB6B: PE-Cy5-5) was performed at room temperature for 30 minutes in PBS (w/o Ca or Mg ...
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bioRxiv - Neuroscience 2023Quote: ... 5 ml of complete Neurobasal medium were added and centrifuged for 5 min at 335 x g (Beckman Coulter Allegra X-12R centrifuge). The complete Neurobasal medium consisted of ...
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bioRxiv - Genomics 2020Quote: ... by ultracentrifugation at 4°C overnight (33500 rpm, Beckman, 70Ti rotor). The ribosome pellet was dissolved in 300 μL resuspension buffer (20 mM Tris-HCl pH 8.0 ...