Labshake search
Citations for Beckman :
601 - 650 of 1226 citations for C Reactive Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2023Quote: ... Pellets were pooled in 1 ml PBS and centrifuged at 100,000 X g for 1,5 h at 4°C in 1,5 ml tubes in a Ti-45 rotor (Beckman Coulter) using adapters (#11004 ...
-
bioRxiv - Cancer Biology 2023Quote: ... the obtained supernatant was filtered through a 0.22 μm filter before ultracentrifugation at 150,000 g for 2 h at 4 °C (Beckman Coulter). The nsEV-containing pellet was resuspended in phosphate buffer saline (PBS).
-
bioRxiv - Biochemistry 2023Quote: ... SV experiments were conducted in charcoal-filled Epon centerpieces with a 12-mm optical path length at 20 °C and at rotor speeds ranging from 38,000 to 48,000 rpm (An-50 Ti rotor, Beckman Coulter). All sedimentation profiles were recorded with either absorption optics at 280 nm or interference optics ...
-
bioRxiv - Microbiology 2023Quote: ... The samples were centrifugated at 70000 rpm for 2 h at 4°C in an Optima TLX Ultracentrifuge (Beckman Coulter ...
-
bioRxiv - Neuroscience 2023Quote: ... NP-40 prepared in DPBS] and then ultracentrifuged at 100,000x g for 1 h at 4 °C using a TLA-55 rotor (Beckman). The supernatant was removed ...
-
bioRxiv - Cell Biology 2023Quote: ... Equilibrated tubes were ultra-centrifuged at 100,000xg for 1h at 4°C using a swinging-bucket rotor (SW 32Ti, Beckman Coulter ...
-
bioRxiv - Cell Biology 2023Quote: ... of proteins were applied on the top of a 16 ml 10-30% continuous sucrose density gradient and centrifuged at 74,400 g for 15 h at 4 °C (SW 32.1 Ti; Beckman Coulter). 24 equal volume fractions were collected ...
-
bioRxiv - Biochemistry 2023Quote: ... at 18000 PSI and cell debris were separated by centrifugation (4 °C, 30 min, 75,600 ×g, JA25.50 rotor Beckman Coulter). The supernatant was loaded on an affinity chromatography column (GE Healthcare ...
-
bioRxiv - Cell Biology 2023Quote: ... Solutions of 15% and 23% Optiprep were layered on top or below the sample and centrifuged at 4°C for 16 h at 120,000g in a SW 28 Ti swinging bucket rotor (Beckman).
-
bioRxiv - Developmental Biology 2023Quote: ... the supernatant was centrifuged in a TLA100.4 fixed angle rotor at 100000 g for 1h at 37°C in an Optima TL ultracentrifuge (Beckman). The supernatant containing globular actin was collected ...
-
bioRxiv - Genetics 2023Quote: ... Fifteen A260 units of WCEs were layered on a pre-chilled 10-50% sucrose gradient and centrifuged at 40,000 rpm for 2 h at 4°C in a SW41Ti rotor (Beckman). Gradient fractions were continuously scanned at A260 using the BioComp Gradient Station and polysome to monosome (P/M ...
-
bioRxiv - Molecular Biology 2024Quote: ... for ultracentrifugation at 100,000 × g for 1 hour at 4°C (JXN-30 centrifuge, JA-30.50 Ti Rotor, Beckman Coulter). The pellet was washed with PBS and centrifuged again at 100,000 × g for 1 hour at 4°C to remove soluble contaminant ...
-
bioRxiv - Neuroscience 2024Quote: ... by centrifugation at 5,000 x g at 4°C for 30 minutes in a fixed angle rotor (Beckman Coulter). The qEV column washed with 1x PBS was then loaded with 500 µL of concentrated supernatant followed by 2.5 mL PBS to collect 3 mL void volume ...
-
bioRxiv - Neuroscience 2024Quote: ... and PBS was added to reach a 4.5 mL tube filling and centrifuged at 120,000xg for 100 min at 4°C in the L-60 Optima Ultracentrifuge (Beckman Coulter ...
-
bioRxiv - Neuroscience 2024Quote: ... Ultracentrifugation was performed for 2.5 h at 18 °C at 350,000 × g in a type 70 Ti rotor (Beckman Coulter). The interface between the 60 and 40% gradient was extracted along with the 40% layer ...
-
bioRxiv - Biochemistry 2023Quote: ... lysates were centrifuged at 60,000 x g (4 °C) for 45 min (Beckman Avanti JXN-26 (rotor: JA 25.5)) and immediately supplemented with 10 mL pre-washed Ni2+-NTA agarose (QIAGEN ...
-
bioRxiv - Biophysics 2024Quote: ... Each seed mix was clarified using an ultra-centrifuge (Beckman Optima MAX-XP, 90,000 RPM, 4°C, 5 min) then snap-frozen in small aliquots and stored at -80°C ...
-
bioRxiv - Cancer Biology 2024Quote: ... 100 μg/mL cycloheximide) for 2 h at 40,000 g at 4 °C using an SW41 rotor (Beckman Coulter). Gradients were fractionated using Teledyne Isco Tris Peristaltic Pump and fractions were collected and pooled according to the UV trace ...
-
bioRxiv - Cell Biology 2024Quote: ... samples were ultracentrifuged at 160,000 xg for 2 h at 4 °C in ultracentrifuge tubes (Beckman Coulter Cat #328874). The resulting pellet contained the insoluble AMB-1 cell fraction and the supernatant contained the soluble fraction ...
-
bioRxiv - Cell Biology 2023Quote: ... Samples were spun at 200,000 × g at 4°C for 4 h in an MLS-50 rotor (Beckman Coulter), and 60 µL of top layer was collected ...
-
bioRxiv - Cell Biology 2024Quote: ... The supernatant was then subjected to ultracentrifugation at 100,000 x g for 2 h at 4 °C to sediment small EVs (Optima L-80K ultracentrifuge, Beckman Coulter ...
-
bioRxiv - Molecular Biology 2024Quote: ... and 1 M sucrose) at 100,000 rpm (543,000 × g) and 4°C for 1 h with a TLA110 rotor (Beckman Coulter) and an Optima MAX-TL Ultracentrifuge (Beckman Coulter) ...
-
bioRxiv - Microbiology 2020Quote: ... Excess dye was removed by ultracentrifugation of the virus through a 10% sucrose cushion for 2 h at 107,000 × g and 4°C using an SW41 rotor (Beckman Coulter). Labeled virus pellets were resuspended at a viral protein concentration of 1 mg/ml ...
-
bioRxiv - Biochemistry 2021Quote: ... 10 U/mL SuperASE-In and was ultracentrifuged at 100,000 × g for for 16 h at 4 °C in a SW Ti41 rotor (Beckman Coulter). After removing the supernatant ...
-
bioRxiv - Biochemistry 2021Quote: ... The lysate was clarified by ultracentrifugation at 30,000 × g 30 min at 4°C using a Ti45 rotor (Beckman Coulter) twice ...
-
bioRxiv - Biophysics 2020Quote: ... lysate was centrifuged at 5 °C with 13000 rpm for 15 min by Avanti J-E Ultra Centrifuge (Beckman Coulter). Supernatant from centrifugation was filtered with 0.45 μm SFCA syringe filters (Sigma Aldrich ...
-
bioRxiv - Biophysics 2020Quote: ... Escherichia coli BL21 cells overexpressing α-synuclein were collected by centrifugation (20 min, 4000 rpm, 4 °C) in a JLA-8.1000 rotor in a Beckman Avanti J25 centrifuge (Beckman Coulter), resuspended in lysis buffer (10 mM Tris ...
-
bioRxiv - Biophysics 2020Quote: ... The insect cells were harvested after 4 days by centrifugation at 300 g for 10 min at 4 °C in an Avanti J-26S ultracentrifuge (JLA-9.1000 rotor, Beckman Coulter). The cell pellet was resuspended in 5 ml ice-cold phosphate buffered saline (PBS ...
-
bioRxiv - Biophysics 2020Quote: ... and centrifuged at 45000 x g for 60 min at 4 °C in the Avanti J-26S ultracentrifuge (JA-30.50Ti rotor, Beckman Coulter). The cleared cell lysate was incubated in a lysis buffer-equilibrated Ni-NTA column (HisPur Ni-NTA Superflow Agarose ...
-
bioRxiv - Cell Biology 2020Quote: ... Crude mitochondria were loaded on top of the chilled gradient and separated at 100,000 Å∼ g for 1 h at 4°C (SW41 rotor; Beckman). Intact purified mitochondria were recovered from a brown band at around 16% Histodenz™ concentration ...
-
bioRxiv - Cell Biology 2020Quote: ... and the gradient was centrifuged for 60 min at 24,700 × g at 4 °C using a TLS-55 swinging bucket rotor (Beckman Coulter). Purified mouse POS were resuspended in DMEM/Ham’s F-12 containing 2.5% sucrose ...
-
bioRxiv - Cell Biology 2020Quote: ... lysed by sonication (UD-201 ultrasonic disrupter, Tomy Seiko), and centrifuged at 50,000 rpm (70 min, 4°C) with a 70 Ti rotor (Beckman Coulter). GST-His6-tagged LC3B-His12 and GATE-16-His12 proteins in the supernatants obtained were isolated by mixing with COSMOGEL GST-Accept beads (50% slurry ...
-
bioRxiv - Immunology 2021Quote: ... the culture medium containing the lentiviral particles was collected and ultracentrifuged at 25,000 rpm for 90 min at 4 °C using a Beckman SW28 ultracentrifuge rotor (Beckman Coulter) for the obtention of concentrated virus ...
-
bioRxiv - Microbiology 2019Quote: ... Samples were centrifuged at 27,000 x rpm for 16 hrs at 4°C in an Optima L-100 XP ultracentrifuge (Beckman Coulter) using a SW32Ti rotor.
-
bioRxiv - Microbiology 2020Quote: ... sanguinis was grown as described above and centrifuged for 10 minutes at 3,740 x g in an Allegra X-142 centrifuge at 4°C (Beckman-Coulter). The supernatant was discarded and the cells were resuspended in BHI prior to inoculation ...
-
bioRxiv - Biochemistry 2022Quote: ... Viral particles were pelleted at 30,000 rpm for 1.5 h at 25°C in an Optima XPN ultracentrifuge using an SW 32 Ti rotor (Beckman Coulter), resuspended in 500 μL DMEM supplemented with 10% FBS ...
-
bioRxiv - Neuroscience 2021Quote: ... PMCA products were centrifuged at 45,000 rpm for 2.5 h at 4 °C (Optima™ Max Ultracentrifuge, Beckman Coulter, USA), the supernatants discarded and the pellets resuspended in an equal volume of PBS.
-
bioRxiv - Molecular Biology 2021Quote: ... 2 mM DTT and centrifuged for 16 h at 34,000 rpm at 4°C for 16 h using an SW60 Ti rotor (Beckman Coulter). The fractions were subjected to SDS-PAGE and Coomassie blue staining.
-
bioRxiv - Molecular Biology 2021Quote: ... The samples were centrifuged at 34,000 rpm at 4°C for 16 h using an SW60 Ti rotor (Beckman Coulter). Subsequently ...
-
bioRxiv - Molecular Biology 2021Quote: ... 2 mM DTT and centrifuged for 16 h at 34,000 rpm at 4°C for 16 h using an SW60 Ti rotor (Beckman Coulter). The fractions ...
-
bioRxiv - Molecular Biology 2020Quote: ... Gradients were centrifuged at 39 000 rpm for 2.5 h at 4°C in an Optima L-100XP ultracentrifuge (Beckman– Coulter) using the SW41Ti rotor with brake ...
-
bioRxiv - Cell Biology 2020Quote: ... The insect cells were harvested after 4 days by centrifugation at 300 g for 10 min at 4 °C in an Avanti J-26S ultracentrifuge (JLA-9.1000 rotor, Beckman Coulter). The cell pellet was resuspended in 5 ml ice-cold phosphate buffered saline (PBS ...
-
bioRxiv - Cell Biology 2020Quote: ... and centrifuged at 45000 x g for 60 min at 4 °C in the Avanti J-26S ultracentrifuge (JA-30.50Ti rotor, Beckman Coulter). The cleared cell lysate was incubated in a lysis buffer-equilibrated Ni-NTA column (HisPur Ni-NTA Superflow Agarose ...
-
bioRxiv - Molecular Biology 2019Quote: ... for 2 h at 4°C and then further purified using CsCl equilibrium gradient ultracentrifugation at 35,000 rpm (Beckman SW56) for 18 h at 4°C ...
-
bioRxiv - Biophysics 2019Quote: ... was transferred to a new polycarbonate ultracentrifuge tube and centrifuged at 100000 xg for 70 minutes at 4°C (Beckman Coulter Optima L-80 XP Ultracentrifuge ...
-
bioRxiv - Cell Biology 2019Quote: ... all proteins were pre-cleared of aggregates via centrifugation at 80,000 RPM for 15 min at 4°C in a TLA100 rotor (Beckman Coulter).
-
bioRxiv - Biochemistry 2019Quote: ... centrifuged at 2000 × g for 10 min at room temperature and the plasma was separated and stored at −20°C until analysis on clinical chemistry analyzer (Beckman Coulter AU5800 ...
-
bioRxiv - Biochemistry 2019Quote: ... Reactions were spun over 15-30% sucrose gradients made in buffer C with appropriate MgCl2 concentrations at 178,000 g (SW-41 rotor, Beckman Coulter) for 3 hours ...
-
bioRxiv - Microbiology 2021Quote: ... the suspension was stored overnight at 30°C and peptidoglycan was collected by centrifugation at 48,000 rpm in SW 55 Ti rotor (Beckman Coulter) for 2 h at 30°C ...
-
bioRxiv - Molecular Biology 2020Quote: ... The total bacterial lysate was centrifuged at 48,384 x g at 4 °C for 1 hr (Beckman JA-25.50 rotor), and the supernatant was applied to a 5 mL HisPur Ni-NTA resin (Thermo Fisher Scientific ...