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Citations for Beckman :
601 - 650 of 797 citations for 2 Amino 6 chloro 9 3 5 di O p toluoyl beta D 2 deoxyribofurnanosyl purine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... For performing cryogenic transmission electron microscopic imaging EVs were centrifuged and washed in PBS at 100,000 x g for 3 hours at 4°C in a SW41 Ti rotor (Beckman Coulter).
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bioRxiv - Cell Biology 2023Quote: ... The concentrated CFS was then ultracentrifuged at 100,000 ˗ g and 4 °C for 3 h (Optima L-80 XP Ultracentrifuge with Type 70.1 Ti Fixed-Angle Titanium Rotor (Beckman Coulter)) ...
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bioRxiv - Microbiology 2022Quote: ... The sample-containing tube was spun at 347,000 × g and 20°C for 3 hours using Type 70 Ti rotor (Beckman Coulter). 1-mL fractions were collected from the top of the tube ...
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bioRxiv - Microbiology 2022Quote: ... 20 mL of the filtrate was then ultracentrifuged at 150,000 × g at 4°C for 3 h (Beckman Coulter, USA) through a 20% (w/v ...
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bioRxiv - Molecular Biology 2023Quote: ... using 10-40 % sucrose gradients in buffer A after 3 hours of centrifugation at 35,000 rpm and 4 °C in SW41 rotor (Beckman Coulter). To isolate ribosomes for structural analysis ...
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bioRxiv - Molecular Biology 2024Quote: ... The gradient was centrifuged at 54,000 rpm for 3 h at 4 °C using a SW55 Ti rotor (Beckman Coulter). After centrifugation ...
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bioRxiv - Biochemistry 2024Quote: ... Equivalent amounts of cell lysates prepared as described above were loaded onto the sucrose gradients and spun at 35,000 g for 3 hours at 4°C in a SW41 rotor (Beckman Coulter). After centrifugation the polysomes were fractionated and absorbance was monitored at 254 nm for RNA and 680 nm for GFP using the TriaxTM Flowcell (BioComp Instruments) ...
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bioRxiv - Cell Biology 2023Quote: ... transferred to a Corning 250 mL centrifuge tube (430776) and then spun again at ∼1000xg (2000rpm in a Beckman Coulter Allegra 6 with a GH3.8 rotor) for 10min at RT with no brake ...
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bioRxiv - Plant Biology 2021Quote: ... reinhardtii cells were collected at 1,870 × g and 4°C for 5 min (JLA9.1000, Beckman Coulter). The cells were resuspended at 0.125 mg Chl/mL with disruption buffer containing 25 mM MES-NaOH (pH 6.5) ...
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bioRxiv - Immunology 2021Quote: ... The tubes were centrifuged at 10,000 g for 5 min (Microfuge 16, Beckman Coulter, Indianapolis, IN) and the collected media was combined with the previously pipetted media.
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bioRxiv - Biochemistry 2021Quote: ... and centrifuged for 5 h with 116,000 xg at 4°C in a TLA100.3 rotor (Beckman). Binding to the different fractions was assessed by Western blot ...
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bioRxiv - Immunology 2021Quote: ... VLPs were concentrated using ultracentrifuge (TLA100.3 rotor, Beckman; at 72,000 rpm for 1 hour, +5°C) or by ultrafiltration using Amicon Ultra 15 (100 kDa ...
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bioRxiv - Immunology 2021Quote: ... and centrifuged (400×g for 5 min at 4°C) using a swinging bucket rotor (Beckman Coulter Avanti J-15RIVD with JS4.750 swinging bucket ...
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bioRxiv - Microbiology 2022Quote: ... 5 mM CaCl2 buffer background) were placed in a 4 mL UltraClear ultracentrifuge tube (Beckman-Coulter), with the particle sample as the top layer ...
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bioRxiv - Immunology 2022Quote: ... A total of 5 × 105 living or fixed cells were analysed using a Cytoflex cytometer (Beckman Coulter Life Sciences ...
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bioRxiv - Neuroscience 2023Quote: ... for 5 min on a swing bucket centrifuge (Allegra X-12R, Beckman Coulter, Brea, CA, USA). Supernatant was decanted and the pellet was resuspended in 0.25% (vol/vol ...
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bioRxiv - Biophysics 2022Quote: ... microtubules were spun down at 200,000 × g for 5 min using an Airfuge ultracentrifuge (Beckman-Coulter) to exclude free GTP ...
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bioRxiv - Immunology 2022Quote: ... and centrifuged (400×g for 5 min at 4°C) using a swinging bucket rotor (Beckman Coulter Avanti J-15RIVD with JS4.750 swinging bucket ...
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bioRxiv - Cancer Biology 2024Quote: ... and centrifuged at 100,000 × g for 5 hours at 4°C (MLS-50, Beckman Coulter, 326819). Afterwards ...
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bioRxiv - Genetics 2020Quote: ... PCR products were pooled by region of interest using 2-3 μL of each reaction and the resulting pools of products were purified using a 1X Ampure XP cleanup (Beckman Coulter). Purified libraries were pooled and submitted for paired-end 150 cycle sequencing on an Illumina MiSeq ...
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bioRxiv - Genomics 2021Quote: ... Cells were washed with 25 mm supplemented HBSS before depleting the sample of unnucleated cells using IOTest 3 Lysing solution (Beckman Coulter) following provider instructions ...
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bioRxiv - Microbiology 2021Quote: ... triplicate spore preparations were made in 3 ml of 20% glycerol and suspended in Isoton II (Beckman Coulter, Brea, CA, USA). The particle volume was then measured for a minimum of 100 000 particles per sample using a Multisizer 4e Coulter counter (Beckman Coulter ...
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bioRxiv - Cell Biology 2020Quote: ... with densities ranging from 1.10 to 1.15 were pooled and washed in PBS (Beckman Ti50, 3 h at 42 000 rpm).
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bioRxiv - Microbiology 2022Quote: A 3 ml volume of pseudoparticles was pelleted using a TLA-55 rotor with an Optima-MAX-E ultracentrifuge (Beckman Coulter) for 2 hours at 42,000 rpm at 4°C ...
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bioRxiv - Molecular Biology 2022Quote: ... Samples were then enriched in DNA sizes between 200 and 600 bp (corresponding to 1 to 3 nucleosomes) using AMPure XP magnetic beads (Beckman-Coulter), following the manufacturer’s guidelines ...
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bioRxiv - Cell Biology 2020Quote: ... 3 ml of 50% and 3 ml of 40% sucrose) in Ultra-Clear Beckman tubes and centrifuged at 26000 rpm (SW32Ti rotor, Beckman Coulter) for 1 h at 4°C ...
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bioRxiv - Biochemistry 2020Quote: ... and pelleted for 3 min in a swing-out rotor at 1000 rpm in a tabletop centrifuge (Allegra X-30R, Beckman Coulter). The supernatant was aspirated and cells were suspended in the remaining liquid to 1 × 105 – 1 × 107 cells/100 μl ...
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bioRxiv - Microbiology 2020Quote: ... The supernatant was ultracentrifuged at 120,000 g for 3 h at 4°C using an MLS-50 rotor (Beckman Coulter, USA) to collect 120k pellet vesicles ...
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bioRxiv - Biophysics 2021Quote: The aggregation products formed in Eppendorf tubes under agitation conditions at pH 3 and pH 4 were centrifuged using an Optima MAX-XP ultracentrifuge (Beckman Coulter) in a TLA-55 rotor at 40,000 rpm at 20°C for 45 min ...
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bioRxiv - Genomics 2020Quote: ... We carried out the first round of PCR for 3 cycles and purified the reactions with Ampure XP beads (Beckman-Coulter). We then carried out a second round of PCR with primers targeting the P5 and P7 sequences only ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were harvested after 3 days and counted using a Z2 Coulter Particle Count and Size Analyzer (Beckman Coulter, Brea, CA).
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bioRxiv - Cancer Biology 2024Quote: ... The percentage of fluorescence-positive cells was determined initially (baseline reference, passage 0, 3 days after transduction) and all following passages using the CytoFLEX Flow Cytometer (Beckman Coulter). In each passage ...
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bioRxiv - Cell Biology 2023Quote: ... 30% Percoll (v/v)] followed by 3 mL of MRB in a 13 mL ultracentrifuge tube (Beckman Instruments, Inc, CA 331372) and spun at 95,000 x g for 45 min in a TH641 rotor ...
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bioRxiv - Genetics 2023Quote: ... the cell pellet was resuspended in PBS supplemented with 2 % FCS and nucleated cells were counted in methylene blue with 3 % acetic acid on a Vi-Cell XR cell viability counter (Beckman Coulter). 10 × 106 bone marrow cells were resuspended in 200 μl of PBS supplemented with 2 % FCS containing the following antibody solution ...
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bioRxiv - Bioengineering 2022Quote: ... Supernatant (33 mL) was placed on a 3 mL 25% (w/v) sucrose pad in SW28 tube (Beckman, Brea, CA, USA) and centrifuged at 24,000 rpm ...
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bioRxiv - Cancer Biology 2023Quote: Counting of CB CD34+ cells after 3 days of ex vivo co-culture was accurately obtained with flow-count fluorosphere beads (Beckman Coulter), in combination with the following antibodies ...
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bioRxiv - Microbiology 2024Quote: ... and resolved by ultracentrifugation at 36,000 rpm for 3 h at 4°C with the fastest braking in Optima XPN-80 Ultracentrifuge equipped with SW-41Ti rotor (Beckman Coulter). Gradients were fractionated (0.5 mL/fraction ...
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bioRxiv - Immunology 2024Quote: ... Fluorescence signals and TruCount beads were acquired on a 3-laser CytoFLEX using Cytexpert software 2.3 (Beckman Coulter, Brea, CA, USA). Instrument quality control and standardization were performed daily using CytoFLEX Daily QC Fluorospheres (Beckman Coulter #B53230).
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bioRxiv - Cancer Biology 2024Quote: ... washed 3 times with chilled phosphate-buffered saline (PBS) and analyzed by flow cytometry (CytoFLex, Beckman Coulter Inc., Brea, CA, USA)[39 ...
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bioRxiv - Cell Biology 2021Quote: ... The seeds were sedimented by centrifugation at 90,000 rpm for 5 min at 25 °C (Beckman Coulter). The microtubule pellet was resuspended in warm BRB80 buffer and microtubule seeds were stored in the dark at room temperature.
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bioRxiv - Biochemistry 2022Quote: ... RNA was purified by denaturing gel electrophoresis (5% polyacrylamide) and by AMPure XP magnetic beads (Beckman Coulter). The purified RNA was buffer exchanged three times into RNase-free water using 30 kDa cutoff Amicon centrifugal filters (Millipore) ...
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bioRxiv - Immunology 2024Quote: ... mApple-positive cells were sorted 5 days after infection on a Moflo XDP flow cytometer (Beckman Coulter) to obtain mApple-Mem-expressing Nalm6 cell lines.
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bioRxiv - Cell Biology 2024Quote: ... The sample was transferred to a 250 µL ultracentrifuge tube (5 x 20 mm Beckman-Coulter, #342630) and a 50 µL of 10% sucrose cushion was placed below the sample at the bottom of the tube ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Digested-ligated products were purified using 1:5:1 ratio of AMPure XP beads (Beckman Coulter, USA). Size selection was performed using a BluePippin to retain total fragment sizes of 250-500bp and were purified using the QIAquick Gel Extraction Kit (Qiagen ...
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bioRxiv - Microbiology 2024Quote: ... 5 mM MgSO4 and 2.1 M sucrose) and centrifuged at 23000 rpm (Beckman Ultracentrifuge, TLA-100.3 Rotor) for 5 min to remove small mycelial pieces and remaining intact cells ...
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bioRxiv - Biochemistry 2024Quote: ... samples were ultracentrifuged at 100,000 g for 5 minutes at 25°C (Beckman TL100, rotor TL-A100). Supernatants were recovered and the hexapeptide concentrations of were determined by UV absorption as previously described ...
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bioRxiv - Molecular Biology 2020Quote: ... MgCl2 5mM and Tris-Cl 50mM pH 7.4) was added into 3-mL polycarbonate tube for an ultracentrifuge TL100.3 rotor (Beckman Coulter, Brea, CA, USA). The KCl-adjusted ribosome-containing solution was carefully added above the sucrose cushion and tubes were balanced by weight using buffer B (sucrose 250mM ...
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bioRxiv - Molecular Biology 2021Quote: ... Produced amplicons of 3-144 µm eukaryotes and Imitervirales were purified with the magnetic beads (Agencourt AMPure XP beads, Beckman Coulter, Inc.). Finial DNA production was dissolved in 27.5 µL ultrapure water ...
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bioRxiv - Plant Biology 2021Quote: ... sucrose was transferred to the bottom of the tube using a Pasteur pipette and centrifuged at 27,000 rpm for 3 hours at 4° C using an SW28 Ti rotor (Beckman Coulter, Brea, CA). Each pellet containing the virus particles was resuspended in 1 mL resuspension buffer overnight with gentle shaking at 4 °C ...
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bioRxiv - Cell Biology 2021Quote: ... supplemented with 100 μg/ml cycloheximide) and centrifuged for 3 hours at 35,000 rpm in an SW41 rotor in a Beckman L7 ultracentrifuge (Beckman Coulter, Krefeld, Germany). The gradients were simultaneously fractionated on a Density Gradient Fractionation System (#621140007 ...