Labshake search
Citations for Beckman :
551 - 600 of 1842 citations for Dengue Virus Serotype 2 Envelope Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2021Quote: ... to remove cellular debris and purified by ultracentrifugation over a 25% sucrose cushion (28.000 rpm for 2 hours; Beckman Coulter ultracentrifuge).
-
bioRxiv - Microbiology 2019Quote: ... PCR products were verified with a 2% agarose gel electrophoresis and purified using AMPure XP bead (Beckman Coulter, Brea, CA, USA). The purified amplicon library was quantified and sequenced on the MiSeq Platform (Illumina ...
-
bioRxiv - Plant Biology 2019Quote: ... for 16 h before the radiolabel was quantified by scintillation for 2 min using a LS6500 Multi-Purpose Scintillation Counter (Beckman Coulter). CPM values were scaled to the average for the 8 week long day plant sample at 6-9 mm or 8 week long day total samples.
-
bioRxiv - Genetics 2020Quote: ... Reverse crosslinking reactions were incubated 2 h at 65°C and cleaned using Agencourt Ampure XP SPRI beads (Cat #A63880, Beckman Coulter) with a 2x bead:sample ratio ...
-
bioRxiv - Immunology 2021Quote: ... Beckman Coulter Access SARS-CoV-2 IgG assays (Brea, CA, USA) targeting the RBD were performed on UniCel DxI 800 Access Immunoassay System (Beckman Coulter). Index value threshold for positivity was 1 as recommended ...
-
bioRxiv - Immunology 2020Quote: ... erythrocytes in 2 μl blood (collected from the tail tip) were counted using a Z2 Coulter Particle count and size analyser (Beckman Coulter). We determined the healthy range in uninfected C57Bl/6J mice housed in our facility to be 8.8 - 10.5*109 erythrocytes*ml−1 ...
-
bioRxiv - Microbiology 2021Quote: ... and either aliquoted or concentrated by ultracentrifugation using 20% (w/v) sucrose cushions for 2 hours at 25,000 rpm in an SW41 or SW28 rotor (Beckman, Indianapolis, IN). Alternatively ...
-
bioRxiv - Immunology 2020Quote: ... 2) first-round PCRs were set up using a Biomek FX liquid handling system with a 96- well head (Beckman Coulter); 3 ...
-
bioRxiv - Immunology 2021Quote: ... Samples were spun at 76,650 x g for 2 hours at 4° C inside a SW 32i rotor using an Optima L-90K Ultracentrifuge (Beckman Coulter). Purified virus was quantified using a bicinchoninic acid assay (BCA ...
-
bioRxiv - Genomics 2021Quote: ... samples were purified with 45 µl of Agencourt AMPure XP magnetic beads with a sample to beads ratio of 3:2 (Beckman Coulter), normalized by Qubit quantification and pooled to generate a 4 nM library ...
-
bioRxiv - Genomics 2021Quote: ... The samples were then centrifuged at 28,600 rpm for 2 h at 4°C (Beckman Optima XE-90; SW32 Ti rotor). After centrifugation ...
-
bioRxiv - Immunology 2020Quote: ... Vesicles recovered by ultracentrifugation of filtered supernatant at 150,000 x g for 2 h at 4 °C using a Ti 45 rotor (Beckman Instruments, USA), were washed with phosphate-buffered saline (PBS ...
-
bioRxiv - Cell Biology 2022Quote: ... A 12 ml 2 % step Optiprep gradient in LB ranging from 32 % to 10 % was prepared beforehand in Ultra-Clear tubes (Beckman Coulter). Samples were spun for 16 hours at 100000 g at 4 °C ...
-
bioRxiv - Neuroscience 2022Quote: ... layers F1/2 were removed before transferring the combined F3/4 layer into a clean 25mL centrifuge tube (Beckman Coulter, #355618) and diluting in GM buffer (at least 1:4 ...
-
bioRxiv - Microbiology 2023Quote: ... viruses were collected and concentrated by ultracentrifugation (37,000 rpm, 2 h 30, 15 °C; Beckman Type 45 Ti fixed-angle rotor). The virus titer was determined using a plaque assay.
-
bioRxiv - Microbiology 2023Quote: ... the DNA monitored on 2% agarose gel was purified by means of AMPure XT beads (Beckman Coulter Genomics, Danvers, MA, USA) and quantified by Qubit (Invitrogen ...
-
A laboratory framework for ongoing optimisation of amplification based genomic surveillance programsbioRxiv - Genomics 2023Quote: ... PCR amplicons for pools 1 and 2 were combined for each sample and purified with a 1:1 ratio of AMPureXP beads (Beckman Coulter) and eluted in 30μL of sterile water ...
-
bioRxiv - Neuroscience 2023Quote: ... The resulting supernatant was filtered with a membrane pore size of 0.2 μm (Whatman) and centrifuged at 120,000 × g for 4 h at 4 °C in polycarbonate bottle assemblies (Beckman coulter, 355603) in a 70.1 Ti fixed angle rotor (Beckman coulter ...
-
bioRxiv - Biophysics 2023Quote: ... and 12-fold diluted in ice-cold SCL and subjected to ultracentrifugation at 100 000 x gav for 2 h in a Ti 70 fixed-angle rotor (Beckman Coulter). The resulting pellets were resuspended in SCL and freshly used for downstream analysis and never subjected to freezing and thawing.
-
bioRxiv - Bioengineering 2023Quote: ... The gradient tubes were balanced and subjected to centrifugation at 135,000 × g for 2 h at 4 °C using a fixed-angle rotor (TLA-55, Beckman Coulter, USA). After DGUC ...
-
bioRxiv - Bioengineering 2023Quote: ... The gradient tubes were subjected to centrifugation at 135,000 × g for 2 h at 4 °C using a fixed-angle rotor (TLA-55, Beckman Coulter, USA). After DGUC ...
-
bioRxiv - Bioengineering 2023Quote: ... The gradient tube was balanced and subjected to ultracentrifugation at 150,000 × g for 2 h at 4 °C using a swinging-bucket rotor (SW 41 Ti Beckman Coulter, USA).
-
bioRxiv - Neuroscience 2023Quote: ... After PCR the samples were combined into small pool of 2-6 samples and size selection was carried out by adding 0.55x volume of AMPure XP beads (Beckman Coulter, #A68831). The concentrations of purified library pools were quantified with Collibri™ Library Quantification Kit (ThermoFisher ...
-
bioRxiv - Biochemistry 2024Quote: ... 50 mM Tris-HCl pH 7.4) and ultracentrifuged at 250 000 x g for 2 h (Beckman Optima TL 100 Ultracentrifuge). After ultracentrifugation ...
-
bioRxiv - Genomics 2024Quote: ... The crude nuclei were stained with 4,6-diamidino-2-phenylindole (DAPI) and loaded into a flow cytometer (Beckman Coulter, MoFlo XDP). Nuclei were purified by flow sorting and washed as described previously [61] ...
-
bioRxiv - Immunology 2020Quote: ... cells were isolated by FACS-sorting on CD11b+Ly6G+ (neutrophils) and CD11b+Ly6G-Ly6C+ (monocytes) cells (Astrios sorter, Beckman Coulter). BMDMs were generated by culturing BM cells with rmM-CSF (20 ng/ml ...
-
bioRxiv - Cancer Biology 2019Quote: ... Gating and percentage of cells for cell cycle phases were determined using the Kaluza® Analysis software v1.3 (Beckman Coulter).
-
bioRxiv - Genomics 2019Quote: ... prior to sorting naïve T cells (CD4+CD45RO-) and T follicular helper cells (CD4+CD45RO+CD25loCXCR5hiPD1hi) on a MoFlo Astrios EQ (Beckman Coulter).
-
bioRxiv - Neuroscience 2020Quote: ... Cell pellet was resuspended in PBS/2mM EDTA/0.5%BSA and cell fluorescence was measured using CytoFLEX S (Beckman Coulter). Flow cytometry data were analyzed using FlowJo X v10.6.1 software (FlowJo LLC ...
-
bioRxiv - Microbiology 2021Quote: ... the percentages of anti-human IgG postive cells in GFP positive cells were then analyzed using flow cytometer (Cytoflex S, Beckman). LIVE/DEAD fixable aqua (Thermo Fisher Scientific ...
-
bioRxiv - Cell Biology 2021Quote: ... the neurospheres were directly dissociated by addition of trypsin in the wells (0.5% final) and the cell number was measured with an automated cell counter (Z2, Beckman Coulter).
-
bioRxiv - Molecular Biology 2022Quote: ... which was used as a selectable marker for single-cell sorting using fluorescence-activated cell sorting (MoFlo Astrios, Beckman Coulter). The resulting single-cell clones were expanded for 7 days and screened for biallelic HDR by PCR (using primers CEBPA-FKBPV screen_F and CEBPA-FKBPV screen_R ...
-
bioRxiv - Molecular Biology 2022Quote: ... Stained cells were resuspended in sort buffer at 1 × 106/ml for fluorescence analysis on a Cytoflex S cell analyser (Beckman). SARS-CoV-2 Spike variants were generated by site-directed mutagenesis with Q5 polymerase (New England Biolabs ...
-
bioRxiv - Immunology 2022Quote: ... where 1×105 viable cells (Flt3+ and/or CD172ahigh) were sorted using a MoFlo Astrios EQ cell sorter equipped with five lasers (Beckman Coulter Eurocenter SA ...
-
bioRxiv - Molecular Biology 2019Quote: The cell sorting experiments were carried out on the MoFlo Astrios EQ cell sorter with the Summit v6.3 software (Beckman Coulter). Cells in stationary phase were diluted 100 times and grown at 30°C with rigorous shaking (200 rpm ...
-
bioRxiv - Microbiology 2020Quote: We stained and sorted single memory B cells as previously described29 using a MoFlo Astrios EQ Cell Sorter (Beckman Coulter). Briefly ...
-
bioRxiv - Cancer Biology 2022Quote: ... The cells were then filtered through a 70 μm strainer and viability was assessed using ViCell cell counter (Beckman-Coulter). Single cell suspensions containing 3×106 cells/sample were stained using appropriate fluorescent antibody cocktails (Listed in Supplementary Table S1 ...
-
bioRxiv - Cell Biology 2022Quote: ... to obtain a high percentage of transfected cells 24 h post-transfection GFP expressing cells were FACS sorted by MoFlo Astrios (Beckman). Following sorting total RNA was prepared ...
-
bioRxiv - Microbiology 2022Quote: ... coli treated cells were also diluted with PBS and sorted using a MoFlo XDP High-Speed Cell Sorter (Beckman Coulter) equipped with 405 nm ...
-
bioRxiv - Cell Biology 2023Quote: ... GFP-positive cells were isolated into single clones using a cell sorter (MoFlo Astrios EQ; Beckman Coulter, Brea, CA, USA). For the confirmation of each gene KO ...
-
bioRxiv - Cell Biology 2023Quote: ... was performed 24- and 48-hours post-treatment and read using a Vi-CELL XR Cell Viability Analyzer (Beckman Coulter).
-
Formation of a giant unilocular vacuole via macropinocytosis-like process confers anoikis resistancebioRxiv - Cell Biology 2024Quote: ... GFP-expressing cells were sorted into single-cell clones by flow cytometry with a Moflo Astrios EQ instrument (Beckman Coulter). Clones were expanded and assessed for gene knockout by immunoblot analysis.
-
bioRxiv - Cell Biology 2024Quote: ... single GFP-positive clones (i.e. cells expressing Cas9-2A-GFP) were sorted into 96-well plates (MoFlo Cell Sorter, Beckman Coulter). Candidate knockout clones were validated by immunoblot and the lead clone was further validated by mass spectrometry ...
-
bioRxiv - Immunology 2021Quote: ... counted using a Multisizer3 cell counter (Beckman Coulter) and the suspension volume adjusted with media to give a final concentration of 1×106/mL.
-
bioRxiv - Systems Biology 2019Quote: ... Cells were sorted using an Astrios device (Beckman), at 4°C by cooling both the input tube holder and the collection tube rack ...
-
bioRxiv - Immunology 2019Quote: ... Dead cells were excluded with 7AAD (Beckman coulter). Antibodies used for paraffin immunostaining were ...
-
bioRxiv - Pharmacology and Toxicology 2019Quote: ... Cells were sorted by FACS (CytoFLEX, Beckman Coulter) and analyzed using FlowJo software.
-
bioRxiv - Cell Biology 2021Quote: ... Cells were analyzed on CytoFLEX S (Beckman Coulter).
-
bioRxiv - Cancer Biology 2021Quote: ... using a MoFlo Astrios cell sorter (Beckman Coulter). tdTomato+ cells (CGNPs ...
-
bioRxiv - Developmental Biology 2021Quote: ... Interested cells were sorted using MoFlo XDP (Beckman) and collected for RNA extraction.