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Citations for Beckman :
551 - 600 of 1324 citations for 4 Tert Butyldimethylsilyl Oxy Methyl 2 Iodothiazole since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2020Quote: ... and centrifuged at 287,582 x g (90,000 rpm) for 1 hr at 4 °C in a TLA100.2 rotor (Beckman, ref. 362046) in 11×34 mm thick-wall polycarbonate ultracentrifuge tubes (Beckman ...
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bioRxiv - Cell Biology 2022Quote: ... The lysate was clarified by centrifugation (183,960 x g, 88 min, 4°C) in a Type 70Ti rotor (Beckman). The supernatant was mixed with 2 ml of IgG Sepharose 6 Fast Flow beads (GE Healthcare Life Sciences ...
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bioRxiv - Molecular Biology 2022Quote: ... Gradients were spun for 3 hours at 4° C and 36,000 RPM (221,632 x g) on SW41-Ti rotor (Beckman Coulter). Monosome fractions were isolated from each sucrose gradient with Brandel Density Gradient Fractionator using 1.5 mL/min flow speed and 60% CsCl ...
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bioRxiv - Cell Biology 2022Quote: ... The lysate was clarified via centrifugation (66,000 x g, 30 min, 4°C) in a Type 70 Ti rotor (Beckman). The supernatant was mixed with 1.5 ml of anti-FLAG M2 affinity gel (Sigma-Aldrich ...
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bioRxiv - Microbiology 2022Quote: ... sonicated using a sonicator (Smurt, Microtec) and ultracentrifuged at 100,000 x g for 60 minutes at 4°C (Optima XE-90, Beckman). Protein concentration of the bacterial membrane suspension was determined with Bio-Rad protein assay (BioRad).
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bioRxiv - Cell Biology 2022Quote: ... and centrifuged at 10,000g and 4°C for 30 minutes (SW41 rotor, Optima XPN-100 ultracentrifuge; Beckman Coulter, Inc.) to remove any potential large debris ...
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bioRxiv - Biochemistry 2022Quote: ... 5 mM MgCl 2 and 2 mM DTT and centrifuged at 70,000 x g for 20.5 h at 4 °C in an SW28 rotor (Beckman Coulter). Fractions corresponding to the 40S peak were collected ...
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bioRxiv - Biochemistry 2022Quote: ... Gradients were centrifuged at 42,800 x g for 15 h at 4 °C in an SW28 rotor (Beckman Coulter). Fractions of 1 mL were collected using an AKTA system (Cytiva) ...
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bioRxiv - Microbiology 2022Quote: ... at 4°C for 1.5-2h followed by 180,000 x g (Ti 70i rotor; Beckman Instruments, Inc., Fullerton, CA) at 4°C for 1.5-2h and resuspended in 1mL of phosphate buffered saline (PBS ...
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bioRxiv - Biochemistry 2022Quote: ... The crude extract was centrifuged at 200,000 g for 1 h at 4 °C (Beckman Coulter Optima LE-80K). The membrane pellet was homogenized and solubilized in 20 mM HEPES (pH 7.8 ...
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bioRxiv - Genetics 2022Quote: ... The lysate was centrifuged at 2000 rpm for 10 min at 4 °C in a JA-20 rotor (Beckman). The supernatant from the previous step was centrifuged in a Beckman Coulter high speed centrifuge equipped with a JA-20 rotor at 6000 rpm for 20 min at 4 °C ...
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bioRxiv - Neuroscience 2020Quote: ... The gradient was centrifuged at 200,000 × g for 20 h at 4°C (35,000 rpm with # Optima-XE SW41 Beckman Coulter). The gradient was collected in 2-mL fractions ...
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bioRxiv - Neuroscience 2020Quote: ... this second supernatant was centrifuged for 4 hrs at 100,000g (Optima MAX-XP ultracentrifuge, TLA-100.3 rotor, Beckman Coulter). Following ultracentrifugation ...
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bioRxiv - Plant Biology 2020Quote: ... and centrifuged at 131,500 × g for 30 min at 4 °C using a SW 32 Ti rotor (Beckman Coulter). Thylakoid membranes sedimented in 1.3 M sucrose cushion were collected and washed with 25 mM Tricine-KOH (pH 7.8) ...
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bioRxiv - Biochemistry 2022Quote: ... mixed for 10 minutes at 4°C and centrifuged at 25,000 rpm for 45 minutes (Beckman Ti-45 rotor). The supernatant was warmed to 37°C for few minutes until it turned cloudy following which it was centrifuged at 11,000 rpm at room temperature for 10 minutes (Beckman JA-20 rotor ...
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bioRxiv - Systems Biology 2020Quote: ... the 500xg supernatant from enzymatic digestion of cardiovascular tissues underwent further ultracentrifugation at 10,000xg for 10 minutes at 4°C (Beckman Coulter ...
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bioRxiv - Cell Biology 2020Quote: ... The soluble fractions released by MNase were isolated by taking supernatants after centrifugation at 13,894 rpm (16,000 rcf) at 4 °C for 30 min using SX241.5 rotor in Allegron X-30R (Beckman Coulter). The supernatants were collected and layered onto the 10-22 % linear sucrose gradient solution with buffer SG (15 mM HEPES-KOH [pH 7.4] ...
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bioRxiv - Biochemistry 2021Quote: ... Eluted fractions were pooled and subjected to a 2.5 h ultracentrifugation at 135,500 x g at 4 °C using a TLA-55 rotor (Beckman-Coulter). Finally ...
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bioRxiv - Biochemistry 2021Quote: ... Reaction mixtures were incubated for 30 minutes and centrifuged at 100,000g at 4°C for 1 hr using a TLA100 fixed angle rotor (Beckman). The supernatant fraction was carefully removed ...
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bioRxiv - Cancer Biology 2020Quote: ... Lysates were loaded on 15-50% sucrose gradient and centrifuged at 35,000 rpm for 2.5 h at 4°C in a SW41 rotor (Beckman Coulter). Polysomes were separated through a live optical density (OD ...
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bioRxiv - Molecular Biology 2020Quote: ... Ten OD260 units were loaded on a 10–50% sucrose gradient and centrifuged at 187,813 × g for 2.75 h at 4 °C in a SW41Ti rotor (Beckman Coulter). The fractions of the gradient were collected ...
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bioRxiv - Cell Biology 2021Quote: ... The crude extract was clarified by high-speed centrifugation (60 min at 36,000 ×g at 4 °C) by Beckman Coulter centrifuge.
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bioRxiv - Plant Biology 2021Quote: ... A soluble fraction was obtained by ultracentrifugation at 200,000 g for 20 minutes at 4°C on a Beckman Optima Ultracentrifuge (Beckman). Two biological replicates for SEC and IEX column separations were prepared in this manner.
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... IL-4 and IFN-γ concentrations were determined using DTX 880 Multimode Detector (Beckman Coulter, Inc., Brea, CA, USA) set to 450 nm.
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bioRxiv - Biochemistry 2021Quote: ... The gradient was centrifuged at 100,000 g for 16 hours at 4°C (rotor MLS-50, Beckman Optima MAX). Twelve fractions of 400 μL were collected from the top of the gradient and pelleted by UC at 100,000 g for 2 hours at 4°C (Figure 1) ...
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bioRxiv - Biochemistry 2021Quote: ... and 10 mM NH4Cl) and centrifuged for 16 h at 4°C with 18,000 rpm in an SW60Ti rotor (Beckman). Gradients were hand-fractionated and ribosome concentrations were determined by absorbance at 260 nm (A260 ...
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bioRxiv - Biochemistry 2021Quote: ... Samples were centrifuged at 50,000 rpm (∼166,000 × g) for 3h at 4°C using TLS-55 rotor (Beckman Coulter) in an Optima benchtop ultracentrifuge (Beckman Coulter ...
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bioRxiv - Biochemistry 2021Quote: ... Membranes were collected by ultracentrifugation at 220 000 x g for 90 min at 4°C (45Ti rotor, Beckman).
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bioRxiv - Biochemistry 2021Quote: ... and the lysate was clarified by centrifugation at 30,000 x g for 60 min at 4 °C in a JA25.50 rotor in an Avanti J-E centrifuge (Beckman). The clarified lysate was then added to 10 mL of Ni-NTA Agarose beads (Invitrogen ...
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bioRxiv - Cell Biology 2021Quote: ... The lysate was clarified by centrifugation for 30 min at 35,000 rpm at 4°C in a Type 45 Ti rotor (Beckman). Cleared lysate was incubated with 2 mL glutathione-sepharose (Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... The lysate was clarified by centrifugation for 30 min at 35,000 rpm at 4°C in a Type 45 Ti rotor (Beckman). Cleared lysate was incubated with 2 mL Ni-NTA agarose (Qiagen ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were ultracentrifuged at 100,000 g for 30 minutes at 4°C in a Optima XPN-100 ultracentrifuge (Beckman). Supernatants were kept aside as “Supernatant” fraction ...
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bioRxiv - Microbiology 2023Quote: ... All centrifugation steps were performed at 4 °C in an Avanti J-E high-speed fixed-angle centrifuge (Beckman Coulter Life Sciences ...
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bioRxiv - Neuroscience 2023Quote: ... DTT-treated EVs were pelleted at 100,000 x g for 70 min at 4°C in a TLA100 rotor (Beckman). The supernatant ...
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bioRxiv - Neuroscience 2023Quote: ... The prepared gradients were centrifuged at 200,000 x g for 16 h at 4°C in an SW41Ti rotor (Beckman), with no braking during deceleration ...
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bioRxiv - Neuroscience 2023Quote: ... The pellet was resuspended in 4.5 volumes of ice-cold extraction buffer and re- centrifuged at 11,300 RPM for 10 min at 4°C using a Ti45 rotor (Beckman). The supernatant was filtered through a 70 μm pore size cell-strainer and combined with the first supernatant ...
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bioRxiv - Neuroscience 2023Quote: ... The tube was topped up to 23 mL with PBS and centrifuged at 59,300 RPM for 30 min at 4°C using a Ti70 rotor (Beckman). The floating lipid layer and supernatant were discarded and the pellet was transferred to a 2 mL tube in 50 μL PBS per g initial grey matter ...
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bioRxiv - Neuroscience 2023Quote: ... The homogenate was centrifuged at 11,300 revolutions per minute (RPM) for 10 min at 4°C using a Ti45 rotor (Beckman). The supernatant was filtered through a 70 μm pore size cell-strainer and retained on ice ...
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bioRxiv - Neuroscience 2023Quote: ... The supernatant was transferred to fresh tubes and centrifuged at 100,000 x g for 70 min at 4°C using a Ti45 rotor (Beckman). The 100,000 x g pellets were resuspended in 1 mL ice-cold PBS and brought to 60 mL using PBS ...
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bioRxiv - Microbiology 2022Quote: ... Cell debris was removed by centrifugation at 14,800 rpm for 20 minutes 4 °C in F241.5P rotor using 149 Microfuge 22R centrifuge (Beckman Coulter). The supernatant was combined with 100 µL of ANTI-FLAG M2 Affinity Gel (Sigma ...
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bioRxiv - Neuroscience 2023Quote: ... The lysate was centrifuged at 40,000 rpm for 50 min at 4°C (45 Ti fixed-angle rotor, Beckman) and the transporter-Fab complex was isolated by affinity chromatography using Strep-Tactin resin ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were lysed by extrusion using a Teflon Dounce homogenizer and clarified by centrifugation at 30000g for 40 minutes at 4°C using a Ja 25.50 rotor (Beckman). The clarified lysate was incubated with 2 ml of pre-equilibrated Protein C affinity resin (Roche ...
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bioRxiv - Biophysics 2023Quote: ... and the resulting lysate was cleared by centrifugation at 279,288 × g for 10 minutes at 4°C using a tabletop centrifuge unit from Beckman Coulter ...
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bioRxiv - Genomics 2023Quote: ... followed by centrifugation at 31,900 ×g (10 min, 4 °C, JA-20 rotor, Avanti J-26 XP, Beckman Coulter). Next ...
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An adaptive biomolecular condensation response is conserved across environmentally divergent speciesbioRxiv - Cell Biology 2023Quote: ... 500 µL was transferred to a vacuum safe tube spun at 100,000 g for 20 minutes at 4°C (fixed-angle TLA-55 rotor in a Beckman Coulter Optimax tabletop ultracentrifuge) ...
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bioRxiv - Plant Biology 2023Quote: ... Approximately 250 µl of crude EV sample resuspended in 20 mM Tris-HCl pH 6.0 was slowly dispensed on top of the column and centrifuged at 100,000 x g for 17 h at 4 ⁰C (SW 41 Ti rotor, Beckman Coulter ...
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bioRxiv - Biochemistry 2023Quote: ... at 40% on a Branson Digital Sonifier and clarified by centrifugation for 30 minutes at 27,216 × g at 4°C in an SS-34 rotor (Avanti J-25 centrifuge, Beckman).
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bioRxiv - Biophysics 2023Quote: ... placed in an An50Ti rotor and centrifugated 50,000 rpm at 4 °C using an Optima XL-I analytical ultracentrifuge (Beckman). The data were analysed in SEDFIT-16.1c 53 using a c(s ...
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bioRxiv - Cell Biology 2023Quote: ... and 1 mM PMSF) and centrifuged for 15 hours at 4°C with 74,400 xg (SW 32.1 Ti; Beckman Coulter). 24 equal volume fractions were collected from the top and subjected to TCA precipitation ...
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bioRxiv - Cancer Biology 2023Quote: ... and centrifuged (33.500 rpm/4°C/3h, brake off) in a Optima XPN-80 ultracentrifuge (Beckman Coulter, Krefeld, Germany). Sucrose gradients were eluted into a BR-188 density gradient fractionation system (Brandel Inc ...