Labshake search
Citations for Beckman :
551 - 600 of 1136 citations for 3 1 3 Dioxan 2 yl 3' methoxypropiophenone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... and GlyA PE (1:200, Beckman Coulter). Finally ...
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bioRxiv - Cancer Biology 2020Quote: ... CD41 PE-Cy5 (1:200, Beckman Coulter), GlyA PE (1:100 ...
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bioRxiv - Cancer Biology 2020Quote: ... CD41 PE-Cy5 (1:200, Beckman Coulter), GlyA PE (1:100 ...
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bioRxiv - Cancer Biology 2020Quote: ... CD41 PE-Cy5 (1:200, Beckman Coulter), GlyA PE (1:100 ...
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bioRxiv - Cancer Biology 2020Quote: ... CD41 PE-Cy5 (1:200, Beckman Coulter) and GlyA PE (1:200 ...
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bioRxiv - Immunology 2021Quote: ... CD103 (clone HML-1, Beckman Coulter, IM1856U). Cells were stained at antibody dilutions of 1:30 ...
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bioRxiv - Immunology 2024Quote: ... PD1 (PE-Cy5.5, 1/320, Beckman Coulter), IL-4 (PerCP-Cy5.5 ...
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bioRxiv - Microbiology 2021Quote: ... and extracellular vesicles including exosomes and virions were pelleted by ultracentrifugation at 141,000 g at 4 °C for 2 h in a swinging-bucket rotor (SW28; Beckman Coulter, Brea, CA, USA). The pellet was resuspended in filtered PBS and centrifuged at 18,300 g and 4°C for 10 min to remove insoluble aggregates ...
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bioRxiv - Microbiology 2020Quote: ... The clarified supernatant was overlaid onto a 20% (w/v) sucrose cushion in Tris-NaCl-EDTA buffer and centrifuged at 134,000 g for 2 hours (Beckman Coulter 30 Ti rotor). The resulting viral pellet was suspended in VCM by repeat pipetting and aliquots stored in cryovials at minus 70°C until needed ...
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bioRxiv - Genomics 2020Quote: ... were layered onto a 1.8M sucrose cushion and ultra-centrifuged at 24,400 rpm at 4°C for 2 hours using a SW28 swinging bucket rotor (Beckman Coulter Optimal centrifuge #L90K). The nuclear pellet was re-suspended in 2% BSA in 1X PBS ...
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bioRxiv - Microbiology 2020Quote: ... EMVs were obtained by ultracentrifugation from 4 mL of filtrate at 100,000 × g (average centrifugal force) and 4°C for 2 h with an OptimaX centrifuge (Beckman Coulter, Brea, CA, USA). The pellets were resuspended in 400 μL of Dulbecco’s phosphate-buffered saline (DPBS ...
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bioRxiv - Neuroscience 2022Quote: ... The tubes were sealed and centrifuged at 50,000 rpm at 4°C for 2 h in an OptimaTM L-90K ultracentrifuge using the 70.1Ti rotor (Beckman Coulter, Brea, CA, USA). After the centrifugation ...
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bioRxiv - Cell Biology 2020Quote: ... 3 μM or 10 μM from a 2 mM or 10 mM stock solution using a Biomek FX with 384-well head (Beckman Coulter, Indianapolis, IN). The DMSO concentration was kept below or at 0.5% v/v ...
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bioRxiv - Physiology 2022Quote: ... the library preparation step followed the CEL-Seq 2 protocol85: the RT product was digested by ExoI and HinFI and purified using AMpure XP beads (Beckman Coulter™, USA). Then ...
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bioRxiv - Microbiology 2024Quote: ... then the colonies were diluted and inoculated into the Neg MIC EN-2J panel containing β-lactams listed in Table 2 and incubated in MicroScan Walkway40 Plus (Beckman Coulter Inc.). Breakpoint criteria for susceptible (S) ...
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bioRxiv - Molecular Biology 2024Quote: ... Each compound was dissolved in DMSO at a concentration of 2 mM and distributed into an Echo Qualified 384-Well Polypropylene Microplates (Beckman Coulter, 001-14615). Using an Echo 655 Liquid Handler ...
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bioRxiv - Immunology 2021Quote: ... supplemented with protease inhibitor cocktail and 1 mM TCEP and centrifuged at 40,000 RPM for 1 h (45 Ti fixed-angle rotor, Beckman). Supernatant was incubated with amylose beads pre-equilibrated in the same buffer for 1 h at 4 °C and loaded onto a gravity flow column ...
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bioRxiv - Biochemistry 2021Quote: ... 1□mM EDTA) and centrifuged at 25000 rpm for 1 hour at 4°C (Beckman Coulter SW41-Ti rotor). Mitochondria forming a band at the interphase between the 1 M and 1.5 M sucrose were collected and resuspended in 10 mM Tris-HCl pH = 7.5 in 1:1 ratio ...
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bioRxiv - Synthetic Biology 2020Quote: ... Reverse transcription reactions were then purified using Agencourt Ampure XP beads at a 1:1 dilution (Beckman-Coulter, A63881). Some portion of the eluent ...
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bioRxiv - Molecular Biology 2020Quote: ... The cDNA from each reaction was purified using a 1:1 ratio of AMPure XP beads (Beckman Coulter, USA). 5ml cDNA was amplified with Q5 High-Fidelity DNA Polymerase (NEB ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Amplified PCR product was purified using a 1:1 concentration of AMPure XP beads (Beckman Coulter, Brea, CA, USA) and eluted in 30 μL of water ...
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bioRxiv - Genetics 2024Quote: ... Half of amplified DNA was purified with SPRI AMPure XP beads (sample to beads ratio 1:1, Beckman; A63881) and eluted in 15μl Buffer EB (QIAGEN) ...
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bioRxiv - Genomics 2024Quote: ... A 15-minute incubation at room temperature with a 1:1 volume ratio of AMPure XP beads (Beckman Coulter) was performed to concentrate samples ...
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bioRxiv - Molecular Biology 2022Quote: ... Stained cells and fluorescence minus one controls were then resuspended in SB + 1:1000 DNase + 1:1000 Draq7 (Beckman Coulter) as a live/dead stain ...
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bioRxiv - Microbiology 2022Quote: ... Libraries were purified and adapter dimers removed with Agencourt AMPure XP beads purification using a 1:1 library:beads ratio (Beckman Coulter). ChIP-seq libraries were sequenced on the Illumina NextSeq 500 system with a 20% phiX spike-in (Illumina ...
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bioRxiv - Immunology 2023Quote: ... the PCR plate was briefly centrifuged and 25 µl of room-temperature AMPure XP beads (1:1 ratio; Beckman Coulter) was added to each well and incubated for 8 minutes at room temperature ...
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bioRxiv - Immunology 2023Quote: ... The ratio of Ca2+-bound Indo-1 versus Ca2+-free Indo-1 was analyzed using FlowJo 9 software (Beckman Coulter).
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bioRxiv - Genetics 2024Quote: ... and doubly size-selected by 0.4:1 ratio exclusion and 0.8:1 ratio inclusion for 300-500bp library size fragments (AMPure XP magnetic beads, Beckman Coulter). These pooled libraries underwent 2 × 250 bp sequencing (Illumina HiSeq 2500 ...
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bioRxiv - Systems Biology 2024Quote: ... Amplified 16S rDNA was purified using a 1:1 volume of Agencourt AmPure XP beads (Beckman-Colter, Brea, CA, USA). The purified products were pooled in equal amounts and analyzed using Illumina MiSeq sequencing ...
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bioRxiv - Microbiology 2022Quote: ... The success of the amplifications was checked in a 2% agarose gel and PCR products were subsequently purified using Agencourt AMPure XP magnetic beads kit (Beckman Coulter, Brea, CA, USA), quantified with the QuantiFluor™ dsDNA System (Promega ...
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bioRxiv - Microbiology 2020Quote: ... and layered on top of a two-step sucrose gradient (20%/50% w/v in HNE) and centrifuged for 2 h at 154 000 x g at 4 °C in a SW41 rotor (Beckman Coulter, Woerden, the Netherlands) to clear from cell debris ...
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bioRxiv - Biochemistry 2023Quote: ... cells were treated with the drugs (20 µM to a suspension at 500,000 cells/mL in PBS containing 2 % FBS at 37°C) and directly analyzed by flow cytometry (Gallios, Beckman-Coulter, 405/488 nm laser) over 2 h ...
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bioRxiv - Microbiology 2024Quote: ... EMVs were obtained by ultracentrifugation from 3 mL of filtrate at 100,000 × g (average centrifugal force) and 4 °C for 2 h with 50Ti rotor and Optimax centrifuge (Beckman Coulter, Brea, CA, United States). The supernatant after removing EMVs was collected as PVF ...
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bioRxiv - Immunology 2022Quote: ... and anti-HIV-1 p24 PE (Beckman coulter) were used ...
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bioRxiv - Neuroscience 2021Quote: ... in 1 ml polycarbonate ultracentrifuge tubes (Beckman Coulter) and centrifuged at 250,000 g for 1 hour at room temperature with a TLA 120.2 rotor using an Optima XP benchtop ultracentrifuge (Beckman Coulter) ...
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bioRxiv - Immunology 2021Quote: ... CD3-PB (1:20; clone UCHT1; Beckman Coulter), CD4-PE-Cy7 (1:100 ...
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bioRxiv - Cancer Biology 2024Quote: ... PE or FITC (J33, Beckman Coulter; 1:10) or CD3-APC ...
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bioRxiv - Cancer Biology 2024Quote: ... PE or FITC (UCHT1; Beckman Coulter; 1:10) were used to stain T-cells in co-culture assays ...
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bioRxiv - Developmental Biology 2023Quote: ... Samples were 1:1.3 AMPure XP (Beckman Coulter) purified and sent for sequencing ...
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bioRxiv - Molecular Biology 2024Quote: ... for 1 h in a Ti45 rotor (Beckman). The supernatant was loaded onto anti-FLAG G1 affinity resin (GenScript ...
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bioRxiv - Genomics 2024Quote: ... 4°C for 1:30h (Beckman Coulter SW32Ti). The pellet was ultimately resuspended with StemSpanTM SFEM-II media (Stemcell Technologies ...
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bioRxiv - Genomics 2020Quote: ... followed by two size-selection steps with SPRIselect (1:0.6 and 1:0.2 sample vol. to beads vol.; Beckman Coulter, CA, USA). The libraries’ size distribution was determined by Bioanalyzer (Agilent Technologies ...
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bioRxiv - Bioengineering 2021Quote: ... containing 10% FBS and 1% PS and 50 nL of FDA-approved compounds (final concentration: 1 μM) using a pin tool (Biomek FX, Beckman Coulter). Cells were added to each well (1,500 cells/25 μL ...
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bioRxiv - Microbiology 2020Quote: ... Libraries were purified and adapter dimers removed with Agencourt AMPure XP beads purification using a 1:1 library:beads ratio (Beckman Coulter, #A63880). ChIP-seq libraries were sequenced on the Illumina NextSeq 500 system with a 20% phiX spike-in (Illumina ...
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bioRxiv - Immunology 2022Quote: ... following manufacturer’s instructions with the following p24 antibodies: HIV-1 p24 clone 28B7-APC (Medimabs) and HIV-1 p24 clone KC57-PE (Beckman Coulter). Acquisition was performed on the Fortessa LSR flow cytometer (BD Bioscience ...
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bioRxiv - Immunology 2024Quote: ... The product of the reverse transcription reaction was purified with AMPure XP beads at a 1:1 bead to sample ratio (Beckman Coulter). Purified cDNA was split in half to amplify the heavy and light chains in separate reactions ...
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bioRxiv - Genomics 2023Quote: ... and paramagnetic beads (1:1 beads/sample) using the same purification protocol as with AMPure XP beads (Beckman Coulter, no. B37419AB). The product purity was assessed via capillary electrophoresis on a QIAxcel instrument (Qiagen ...
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bioRxiv - Microbiology 2024Quote: ... 2 µL per sample was used to pool the plates followed by concentrating using a 1:1 AMPure XP Beads clean up (Beckman Coulter). Beads were rinsed using two washes of 80% ethanol with 180-degree turns ...
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bioRxiv - Cancer Biology 2020Quote: ... Filtered media containing lentiviral particles were centrifuged at 24000rpm at 20°C for 2 hrs in 90Ti rotor in ultra-centrifuge (Beckman Coulter Optima TM L-100K). Viral particle pellet was obtained after discarding supernatant post centrifugation and suspended in 100 μl OptiMEM media for overnight at 4°C.The collected lentivirus suspension was used for transfection or stored for later use at −80°C ...
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bioRxiv - Physiology 2020Quote: ... one bird per pen in Experiment 2) and centrifuged for 5 min at 17013 × g using a Beckman microfuge (Model E 348720, Beckman Instruments, INC, Palo Alto, CA). Then the viscosity of ileal supernatant was measured using a Brookfield cone-plate digital viscometer (Model LVDV-Ⅲ ...