Labshake search
Citations for Beckman :
551 - 600 of 1703 citations for 2H Pyrazolo 4 3 c pyridine 3 chloro 4 5 6 7 tetrahydro since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2023Quote: ... Samples were then centrifuged at 82,600×g at 4°C for 2 h (Beckman ultracentrifuge optima L-100 XP). After the first ultracentrifugation ...
-
An adaptive biomolecular condensation response is conserved across environmentally divergent speciesbioRxiv - Cell Biology 2023Quote: ... 500 µL was transferred to a vacuum safe tube spun at 100,000 g for 20 minutes at 4°C (fixed-angle TLA-55 rotor in a Beckman Coulter Optimax tabletop ultracentrifuge) ...
-
bioRxiv - Microbiology 2023Quote: ... The denatured protein fraction was then removed via ultracentrifugation at 100,000 g for 20 min at 4°C (Beckman Coulter Optima XE-90 – IVD ultracentrifuge with a 42.2 Ti rotor) ...
-
bioRxiv - Plant Biology 2024Quote: ... the supernatant after the 40,000 g spin was transferred to ultracentrifuge tubes and centrifuged at 100,000 g for 1 h at 4 °C (TLA100.3 rotor, Optima TLX Ultracentrifuge; Beckman Coulter). P40 and P100-P40 pellets were resuspended in cold and filtered VIB or 100 mM Tris pH 7.4 and either used immediately or stored at –80 °C until further use.
-
bioRxiv - Plant Biology 2024Quote: ... tubes and centrifuged at 40,000 g for 1 h at 4 °C (TLA100.3 rotor, Optima TLX Ultracentrifuge; Beckman Coulter). Where indicated ...
-
bioRxiv - Cell Biology 2024Quote: ... The lysate was clarified by centrifugation (183,960 x g, 88 minutes, 4 °C) in a Type 70Ti rotor (Beckman). The supernatant was mixed with 2 mL of IgG Sepharose 6 Fast Flow beads (Cytiva ...
-
bioRxiv - Cell Biology 2024Quote: ... The lysate was clarified via centrifugation (66,000 x g, 30 minutes, 4 °C) in a Type 70 Ti rotor (Beckman). The supernatant was mixed with 1.5 mL of α-FLAG M2 affinity gel (Sigma–Aldrich ...
-
bioRxiv - Cell Biology 2024Quote: ... The lysate was clarified by centrifugation (66,000 x g, 30 minutes, 4 °C) in a Type 70Ti rotor (Beckman). For His-ZZ-TEV-Halo-CCSer2650–850 ...
-
bioRxiv - Biochemistry 2024Quote: ... dimethylammonio)-1-propanesulfonate)] and lysates were cleared by centrifugation at 75,600 g at 4 °C for 30 min using a JA 25.50 rotor (Beckman-Coulter). Next ...
-
bioRxiv - Neuroscience 2024Quote: ... EV-depleted FBS medium was obtained by the ultracentrifugation of BV-2 complete medium supplemented with 20% FBS for 18h at 100.000 × g and 4°C in 38.5 mL polyallomer Ultra Clear ultracentrifuge tubes (Beckman Coulter), using an Optima XPN-80 ultracentrifuge and an SW 32 Ti Rotor (Beckman Coulter ...
-
bioRxiv - Molecular Biology 2024Quote: ... The samples were ultracentrifuged at 100,000 × g for 135 min at 4°C (MLA-55 rotor, Beckman Coulter, USA). After removal of the supernatant ...
-
bioRxiv - Cell Biology 2024Quote: ... Solutions of 15% and 23% Optiprep were layered on top or below the sample and centrifuged at 4°C for 16 h at 120,000g in a SW 28 Ti swinging bucket rotor (Beckman).
-
bioRxiv - Cell Biology 2024Quote: ... The gradients were centrifuged at 39,000 rpm for 2.5 h at 4°C using a SW41Ti rotor (Beckman Instruments), and 21 fractions of 0.5 ml were collected ...
-
bioRxiv - Microbiology 2024Quote: ... were centrifuged at approximately 108,000 x g for 107 min at 4°C using an ultracentrifuge device equipped with a TLA 100.3 fixed-angle rotor (Beckman). The supernatants were retained as soluble fractions and kept at 4°C for downstream analyses ...
-
bioRxiv - Microbiology 2024Quote: ... Phage particles were pelleted by centrifugation at 75,000 x g at 4°C for 1 h using a 50.2 Ti rotor (Beckman Coulter) and resuspended in the phage buffer.
-
bioRxiv - Neuroscience 2024Quote: ... The homogenate was layered on the gradient and centrifuged (3h, 4°C, 160,000 xg) using a SW40 rotor (Beckman). Top layer of debris was removed and ∼18 fractions of 500 µl were collected from each gradient from top (fraction 1 ...
-
bioRxiv - Molecular Biology 2024Quote: ... were layered on top of each sucrose gradient and centrifuged (41,000 rpm for 2 hours at 4°C) in an SW41 Ti rotor (Beckman). UV profiling (254 nm ...
-
bioRxiv - Biophysics 2024Quote: ... Lysates were clarified by ultracentrifugation at 34,000 rpm for 45 minutes at 4°C in a Type 45.1 Ti rotor (Beckman-Coulter). The supernatants were then passed through a 0.22 μm syringe sterile filter (Thermo-Fisher ...
-
Syntaxin-2 inhibits excessive phagocytic uptake but promotes phagolysosomal clearance in macrophagesbioRxiv - Cell Biology 2024Quote: ... The samples were then centrifuged at 100,000 g for 1 hr at 4°C using a SW40Ti rotor (Beckman). Phagosomes were recovered from the interface of 10 and 25% sucrose (Fig ...
-
bioRxiv - Cell Biology 2024Quote: ... and the membranes were pelleted at 100,000 g for one hour at 4°C in a TLA 100.3 rotor (Beckman-Coulter). Afterwards the supernatant was taken off and stored at −20°C (“S100” ...
-
bioRxiv - Biochemistry 2024Quote: ... The unbroken cells and debris were removed by centrifugation at 20,000 x g for 10 min at 4 °C (Beckman Coulter Avanti J20 XPI centrifuge ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... containing cell nucleus and other heavy organelles was discarded and supernatant was centrifuged for 30 min at 48,000 × g at 4 °C (Beckman Avanti J-251 Ultra-centrifuge ...
-
bioRxiv - Neuroscience 2024Quote: ... The supernatant was passed through a 0.22 μm filter before undergoing ultracentrifugation at 100,000g for 70 min at 4°C (Optima-XE SW41 Beckman Coulter). The resulting pellet was then resuspended in 2 ml of 0.475 M sucrose solution prepared in double-filtered PBS ...
-
bioRxiv - Immunology 2024Quote: ... Sartorus) at 100,000 x g for 70 min at 4°C using the ultracentrifuge Optima MAX-TL (Beckman Coulter). The pellets were suspended in PBS(– ...
-
bioRxiv - Bioengineering 2024Quote: ... for 15 minutes and centrifuged at 10,000 x g for 25 minutes at 4°C (JA20 rotor, Beckman Coulter). The supernatant containing the recombinant proteins was filtered through a 0.45 µm sterile filter (Whatman Puradisc) ...
-
bioRxiv - Biophysics 2024Quote: ... The supernatant was then centrifuged for 1 h at 38000 rpm and 4 °C in a TI45 rotor (Beckman) to pellet the membranes ...
-
bioRxiv - Biophysics 2024Quote: Tau proteins were diluted in cold 1x BRB80 to a concentration of a few tenths of micromolar and centrifuged to remove aggregates (10 min/4°C /215,000 x g in Type 70 Ti rotor [Beckman]). Prior to use ...
-
bioRxiv - Biophysics 2024Quote: ... The lysate was clarified via centrifugation (66,000 x g, 30 min, 4°C) in a Type 70 Ti rotor (Beckman), before being incubated overnight at 4°C with 1.5 mL of anti-FLAG M2 affinity gel (Sigma-Aldrich ...
-
bioRxiv - Biochemistry 2024Quote: ... The crude membrane fraction containing MsbA was isolated by ultracentrifugation (100000g, 45 min, 4°C, Beckman Coulter rotor Ti70). About 2 mg of MsbA-enriched membranes were solubilized with 1% DDM (w/v ...
-
bioRxiv - Cancer Biology 2024Quote: ... Samples were centrifuged at 24,000 rpm under vacuum for 1.5 hrs at 4°C (Allegra X-15R Centrifuge, Beckman Coulter). The viral pellet was resuspended in 300 µL PBS ...
-
bioRxiv - Biophysics 2024Quote: ... Lysis was accomplished via douncing and the lysate was clarified via centrifugation (183,960 x g, 88 min, 4°C) in a Type 70Ti rotor (Beckman). 2 mL of IgG Sepharose 6 Fast Flow beads (Cytiva ...
-
bioRxiv - Cell Biology 2024Quote: ... and the lysate was cleared twice by centrifugation at 40,000 rcf for 20 min each at 4°C using a JA 25.50 rotor (Beckman Coulter). The cleared lysate was incubated with 2 mL Ni-NTA resin (Thermo Fisher Scientific ...
-
bioRxiv - Cancer Biology 2024Quote: ... samples were loaded based on equal total RNA onto a 10%–50% sucrose gradient and subjected to ultracentrifugation (187,813 g for 1.5 hours at 4 °C) using a SW 41 Ti rotor (Beckman Coulter). Subsequently ...
-
bioRxiv - Bioengineering 2024Quote: ... The virus was then pelleted by centrifugation at 4°C for 45 min at 25,000 rpm in a sw28 rotor (Beckman) and gently suspended in dilution buffer (150 mM NaCl ...
-
bioRxiv - Cancer Biology 2024Quote: ... which was then centrifuged at 100,000 × g for 18 hours at 4°C (344060, SW 40 Ti, Beckman Coulter) for further purification ...
-
bioRxiv - Cell Biology 2024Quote: ... All ultracentrifugation steps will be performed at 4°C using a SW41 Ti rotor (Beckman Coulter, Brea, CA, USA).
-
bioRxiv - Evolutionary Biology 2024Quote: ... Ribosomes were pelleted by centrifugation at 27,000 rpm (57,000 xg) for 16 h at 4 °C in a Ti-45 rotor (Beckman-Coulter). Ribosomes pellets were then resuspended in dissociation buffer (20 mM Tris-HCl pH 7.5 ...
-
bioRxiv - Cell Biology 2024Quote: ... Cells were harvested at day 3 post-infection by centrifuging at 2,000xg for 15 mins at 4°C using a JLA 8.1000 rotor (Beckman Coulter). Insect cell pellets were frozen in liquid nitrogen for storage or used immediately for protein purification.
-
bioRxiv - Cell Biology 2024Quote: ... Cells were harvested by centrifuging at 5,000xg for 15 mins at 4°C using a JLA 8.1000 rotor (Beckman Coulter). Bacterial cell pellets were frozen in liquid nitrogen for storage or used immediately for protein purification.
-
bioRxiv - Cell Biology 2024Quote: ... Lysates were clarified by centrifugation at 20,000 x g for 45 mins at 4°C in a JA25.50 rotor (Beckman Coulter). Clarified lysates were applied to a gravity flow column several times over 2 ml Ni-NTA resin (Sigma ...
-
bioRxiv - Immunology 2020Quote: ... KIR2DL2/3/S2 (GL183, PE/Cy5.5, Beckman Coulter,), NKG2C (134591 ...
-
bioRxiv - Systems Biology 2023Quote: ... enumerated using a Multisizer 3 Coulter Counter (Beckman,), and then seeded at the desired density in 6-well tissue culture plates (TPP ...
-
bioRxiv - Microbiology 2020Quote: ... and overlaid with 6 ml of 30% and 3 ml of 5% sucrose (w/v) followed by centrifugation at 32,000 rpm in SW41 rotor (Beckman Coulter) for 20 h ...
-
bioRxiv - Neuroscience 2020Quote: ... reverse 5’-CTTA TTAGTCAGTCAGAAACAAC-3’).ThePCR amplicon was purified using RNAse-free SPRI beads (Agencourt RNAclean XP, Beckman-Coulter A63987) and then used for in vitro transcription with the HiScribe T7 ARCA mRNA Kit (with tailing ...
-
bioRxiv - Cancer Biology 2024Quote: ... The fragments were repaired the 3’and 5’overhangs using End repair mix (component of SureselectXT) and purified using Agencourt AMPure XP Beads (Beckman). The purified fragments were added with ‘A’ tail using A tailing Mix (component of SureSelectXT ...
-
bioRxiv - Pathology 2020Quote: ... Hemocytes stained with 4’-6-diamidino-2-phenylindole (DAPI) were analyzed on a CytoFLEX flow cytometer with CytExpert software (Beckman-Coulter, USA). Plots of side scatter SSC-A versus forward scatter FSC-A were used for visualization of cell groups ...
-
bioRxiv - Cancer Biology 2024Quote: ... The plates were then centrifuged at 2000 rpm (931 x g) for 2h at 30°C (Allegra® X-15R Centrifuge (Beckman Coulter), rotor SX4750A) ...
-
bioRxiv - Microbiology 2020Quote: ... Excess dye was removed by ultracentrifugation of the virus through a 10% sucrose cushion for 2 h at 107,000 × g and 4°C using an SW41 rotor (Beckman Coulter). Labeled virus pellets were resuspended at a viral protein concentration of 1 mg/ml ...
-
bioRxiv - Biochemistry 2021Quote: ... 10 U/mL SuperASE-In and was ultracentrifuged at 100,000 × g for for 16 h at 4 °C in a SW Ti41 rotor (Beckman Coulter). After removing the supernatant ...
-
bioRxiv - Biochemistry 2021Quote: ... The lysate was clarified by ultracentrifugation at 30,000 × g 30 min at 4°C using a Ti45 rotor (Beckman Coulter) twice ...