Labshake search
Citations for Beckman :
501 - 550 of 805 citations for Rabbit Anti Sheep IgG H+L FITC since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... filtered supernatant was underlaid with 20% sucrose PBS using a pipetting needle and centrifuged at 39 000 rpm for 2 h using an SW-41 rotor (Beckman Coulter). The supernatant and sucrose solution were carefully removed ...
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bioRxiv - Microbiology 2022Quote: ... The virus suspension was pelleted through a 40% sucrose cushion by centrifugation for 2 h at 40,000 rpm in a SW55 Ti rotor (Beckman, Fullerton, CA) at 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... sucrose gradient and the RCII complexes were separated by ultracentrifugation (40,000×g,15 h) using an Optima XPN-90 Ultracentrifuge (Beckman Coulter, USA). The separated RCII/PSI fraction was collected and then washed repeatedly using the glycerol- and DM-free elution buffer and concentrated to 0.2 mg Chl/ml which corresponded to approximately 0.6 mg protein/ml.
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bioRxiv - Microbiology 2022Quote: ... Samples were incubated for 2 h at 16°C and double stranded cDNA was purified using AMPure XP beads (Beckman Coulter). For testing transcription activity of predicted genes ...
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bioRxiv - Molecular Biology 2019Quote: ... EDS was obtained after 18 h ultracentrifugation at 100,000 g and 4 °C (SW55 Ti rotor, Beckman Coulter, Fullerton, California, USA), followed by 0.22 µm filtration ...
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bioRxiv - Molecular Biology 2019Quote: ... followed by 3 h ultracentrifugation at 100,000 g and 4 °C using SW 32.1 Ti rotor (Beckman Coulter, Fullerton, California, USA). Resulting pellets were resuspended in 100 µl PBS and stored at −80 °C until further use ...
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bioRxiv - Cell Biology 2019Quote: ... Protein samples were overlaid on the gradients and the tubes centrifuged for 18 h at 37000 rpm using a SW 41 Ti rotor (Beckman Instruments), at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... Reconstituted nucleosome samples were overlaid onto the sucrose gradient and spun at 32000 rpm for 20 h at 4 ° using SW55Ti rotor (Beckman Coulter) in Beckman Optima L80 ...
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bioRxiv - Microbiology 2020Quote: ... The supernatant was ultracentrifuged at 120,000 g for 3 h at 4°C using an MLS-50 rotor (Beckman Coulter, USA) to collect 120k pellet vesicles ...
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bioRxiv - Plant Biology 2019Quote: ... for 16 h before the radiolabel was quantified by scintillation for 2 min using a LS6500 Multi-Purpose Scintillation Counter (Beckman Coulter). CPM values were scaled to the average for the 8 week long day plant sample at 6-9 mm or 8 week long day total samples.
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bioRxiv - Genetics 2020Quote: ... Reverse crosslinking reactions were incubated 2 h at 65°C and cleaned using Agencourt Ampure XP SPRI beads (Cat #A63880, Beckman Coulter) with a 2x bead:sample ratio ...
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bioRxiv - Microbiology 2020Quote: ... Vesicles were obtained by centrifugation at 38,400 x g for 1 h at 4°C in an Avanti J-20XP centrifuge (Beckman Coulter, Inc). Pelleted vesicles were resuspended in 20 ml of 50 mM HEPES (pH 6.8 ...
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bioRxiv - Genomics 2021Quote: ... The samples were then centrifuged at 28,600 rpm for 2 h at 4°C (Beckman Optima XE-90; SW32 Ti rotor). After centrifugation ...
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bioRxiv - Molecular Biology 2021Quote: ... mtEFG1 was subsequently removed from the mitoribosomes via a 10 – 40 % (w/v) sucrose gradient in 1x MB (17 h, 22500 rpm, SW 32 Ti rotor (Beckman Coulter), 4 °C) ...
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bioRxiv - Immunology 2020Quote: ... Vesicles recovered by ultracentrifugation of filtered supernatant at 150,000 x g for 2 h at 4 °C using a Ti 45 rotor (Beckman Instruments, USA), were washed with phosphate-buffered saline (PBS ...
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bioRxiv - Cell Biology 2022Quote: ... After ultracentrifugation at 200,000 x g for 5 h at 4°C (SW55Ti rotor in a XL-90 Beckman Coulter centrifuge), fractions containing purified EVs or purified phages were extracted from the top following the scheme in Supp ...
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bioRxiv - Microbiology 2023Quote: ... The isopycnic centrifugation of DNA was performed with an initial CsCl buoyant density of 1.725 g mL-1 subjected to centrifugation at 177,000 × g for 36-40 h at 20 °C in an Optima XPN-80 ultracentrifuge (Beckman Coulter, USA). At the end of the centrifugation ...
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bioRxiv - Microbiology 2023Quote: ... viruses were collected and concentrated by ultracentrifugation (37,000 rpm, 2 h 30, 15 °C; Beckman Type 45 Ti fixed-angle rotor). The virus titer was determined using a plaque assay.
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bioRxiv - Immunology 2023Quote: ... The live singlets were counted using DAPI 1:10 dilution by mixing 45 μl of cell suspension with 5 μl DAPI by gating on FSC-A vs FSC-H measuring average event/ μl on Cytoflex (Beckman Coulter). Subsequently ...
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bioRxiv - Biophysics 2023Quote: ... the SWCNT suspension was centrifuged at 30000 rpm (164 000 x g) for 4 h at 25 °C (Optima XPN-80, Beckman Coulter) to remove any remaining nanotube aggregates.
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bioRxiv - Biophysics 2022Quote: ... EVs were separated from protein aggregates and free reagents by bottom-up density gradient centrifugation in sucrose for 17.30 h at 192,000 g and 4°C using a SW40 rotor (k-factor 144.5; Beckman Coulter, Fullerton, California, USA). Twelve fractions of 1 mL were then collected from the top of the gradient and respective densities were determined by refractometry using an Atago Illuminator (Japan) ...
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bioRxiv - Neuroscience 2023Quote: ... The resulting supernatant was filtered with a membrane pore size of 0.2 μm (Whatman) and centrifuged at 120,000 × g for 4 h at 4 °C in polycarbonate bottle assemblies (Beckman coulter, 355603) in a 70.1 Ti fixed angle rotor (Beckman coulter ...
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bioRxiv - Biophysics 2023Quote: ... The bottom-loaded 45-10% gradient was then centrifuged at 120 000 x gav for 16 h in a SW40 Ti Swinging Bucket rotor (Beckman Coulter). After ultracentrifugation ...
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bioRxiv - Biophysics 2023Quote: ... and 12-fold diluted in ice-cold SCL and subjected to ultracentrifugation at 100 000 x gav for 2 h in a Ti 70 fixed-angle rotor (Beckman Coulter). The resulting pellets were resuspended in SCL and freshly used for downstream analysis and never subjected to freezing and thawing.
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bioRxiv - Microbiology 2023Quote: ... Samples were centrifuged at 35 000 rpm for 18 h in a Beckman SW41 rotor in an OptimaXE-90 Ultra-centrifuge (Beckman Coulter). Eleven fractions were manually collected from each tube ...
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bioRxiv - Bioengineering 2023Quote: ... The gradient tubes were balanced and subjected to centrifugation at 135,000 × g for 2 h at 4 °C using a fixed-angle rotor (TLA-55, Beckman Coulter, USA). After DGUC ...
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bioRxiv - Bioengineering 2023Quote: ... The gradient tubes were subjected to centrifugation at 135,000 × g for 2 h at 4 °C using a fixed-angle rotor (TLA-55, Beckman Coulter, USA). After DGUC ...
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bioRxiv - Bioengineering 2023Quote: ... The gradient tube was balanced and subjected to ultracentrifugation at 150,000 × g for 2 h at 4 °C using a swinging-bucket rotor (SW 41 Ti Beckman Coulter, USA).
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bioRxiv - Cancer Biology 2023Quote: ... in phenol red free media 24 h prior to addition of drugs through an automated robotic liquid handler (BioMek, Beckman Coulter). After 72h ATP-luciferase assay reagent was added (CellTiter-Glo ...
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bioRxiv - Biochemistry 2024Quote: ... 50 mM Tris-HCl pH 7.4) and ultracentrifuged at 250 000 x g for 2 h (Beckman Optima TL 100 Ultracentrifuge). After ultracentrifugation ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were then spun at 100,000 xg for 16 h at 4°C using a TLS-55 swinging-bucket rotor and benchtop ultracentrifuge (Optima Max-XP, Beckman Coulter). Fractions were collected and loaded in SDS-PAGE for WB.
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bioRxiv - Cell Biology 2024Quote: ... The samples were ultracentrifuged at 39,000 rpm at 4°C for 2.5 h in an SW41 rotor (Beckman Coulter, California, USA). Finally ...
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bioRxiv - Microbiology 2024Quote: ... The final Hcp1-VLPs particles were pelletized by ultracentrifugation at 274,000 ×g at 4°C for 1 h in a SW41 Ti rotor (Beckman Coulter, Inc). The final pellet was resuspended in PBS pH 7.4.
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bioRxiv - Microbiology 2024Quote: ... 0.2% Triton X-100 and 100 µg/mL chloramphenicol) and ultracentrifuged at 42500 rpm for 5 h in type 70 Ti rotor (Beckman Coulter) at 4°C ...
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bioRxiv - Molecular Biology 2020Quote: ... followed by ultracentrifugation at 200000 g for 120 minutes (Type 70 Ti rotor, Optima L-80 XP, Beckman Coulter). The pellet was washed in PBS and collected by ultracentrifugation at 200000 g for 120 minutes [17] ...
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bioRxiv - Biochemistry 2019Quote: ... 15 mM imidazole and lysed by sonication in the presence of 10 μL of 1 U.ml-1 DNase I per litre of over-expression culture and cell lysate was clarified (Beckman JA25.50 rotor ...
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bioRxiv - Microbiology 2020Quote: ... supernatants were harvested and filtered through a 0.45μm filter and centrifuged at 27,000 rpm for 3h at 4°C with a SW41 rotor and Optima L-90 centrifuge (Beckman). Pellets were resuspended in PBS prior to use for western blot analysis.
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bioRxiv - Microbiology 2021Quote: ... and 500 μl of a 10 ml resuspension of cells from each flask diluted with 19.5 ml Isoton II Diluent (Beckman). PD time ...
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bioRxiv - Microbiology 2020Quote: ... density gradient centrifugation was performed in 5,1 ml quick-seal tubes in a NVT 65.2 rotor (near vertical) using Optima L-90K centrifuge (Beckman coulter). CsCl medium having an average density of 1,72g/ml was loaded with 6μg of total extracted DNA ...
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bioRxiv - Cancer Biology 2023Quote: ... Samples were sedimented onto poly-L-lysine coated coverslips at 12,000 rpm (22,579 x g) in an Avanti J-25 centrifuge (Beckman Coulter) for 1 hr and post-fixed with methanol for 5 min at −20 °C ...
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bioRxiv - Pathology 2023Quote: ... and culture supernatant was concentrated by ultracentrifugation (2h at 26,000rpm; Ultraclear Tubes; SW28 rotor and Optima L-100 XP Ultracentrifuge; Beckman). Viruses were suspended in cold sterile PBS and titrated by transduction of Jurkat cells for 48h ...
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bioRxiv - Neuroscience 2024Quote: ... and PBS was added to reach a 4.5 mL tube filling and centrifuged at 120,000xg for 100 min at 4°C in the L-60 Optima Ultracentrifuge (Beckman Coulter ...
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bioRxiv - Biophysics 2020Quote: ... The lysates were clarified by centrifugation (16,000 rpm for 1 h at 4°C, rotor JA 25.50, Beckman Coulter, Brea, California, USA). The supernatant was incubated with amylose resin for 1 h at 4°C and loaded onto a 20 ml chromatography column (Bio-Rad ...
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bioRxiv - Biophysics 2020Quote: ... Very small amounts of fibrillar species formed during this time were removed by ultracentrifugation for 1 h at 90000 rpm (Optima TLX Ultracentrifuge, Beckman Coulter, TLA-120.2 Beckman rotor, 288000 g). The excess monomeric protein and some small oligomers were then removed by multiple filtration steps using 100-kDa cut-off membranes ...
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bioRxiv - Immunology 2021Quote: ... The pelleted exosomes from above supernatants were further centrifuged at 100,000g (26,450rpm) for 2 h at 4 °C (Beckman Coulter, Optima XE-90) and resuspended in PBS ...
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bioRxiv - Microbiology 2021Quote: ... Samples were centrifuged at 130,000 × g for 2 h at 4°C in a TLA100.3 rotor (Beckman Optima TLX table top centrifuge). Following centrifugation ...
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bioRxiv - Neuroscience 2021Quote: ... The post nuclear supernatant was transferred to an ultracentrifuge tube and centrifuged for 2 h at 245,000 g at 4 °C in a Beckman L90 Ultracentrifuge (Beckman Coulter, Mississauga, Canada) to pellet remaining membrane.
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bioRxiv - Molecular Biology 2019Quote: ... was loaded onto 4 ml of a 10% −30% (w/v) linear sucrose gradient and centrifuged at 74,000g at 4°C for 6 h (Beckman MLS-50 rotor). Fractions (250 µl ...
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bioRxiv - Molecular Biology 2019Quote: ... The DG was centrifuged 18 h at 100,000 g and 4 °C using SW 32.1 Ti rotor (Beckman Coulter, Fullerton, California, USA). Density fractions of 1 ml were collected and fractions 9-10 pooled ...
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bioRxiv - Biochemistry 2020Quote: ... to a final volume of 1 ml and then further centrifuged (120000 g, 4°C, 2 h) in a TLA-120.2 rotor (Beckman Coulter, Brea, CA). Pellets were washed twice with 4°C cold PBS and centrifuged (120000 g ...