Labshake search
Citations for Beckman :
501 - 550 of 885 citations for Pregnanediol 3 Glucuronide PDG ELISA Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2023Quote: ... 80 µl aliquots of the soil solution diluted in each medium were dispensed into the wells of 384-well plates using a Biomek liquid handler (Beckman Coulter, Indianapolis, IN, USA). Three plates per corrinoid condition were inoculated ...
-
bioRxiv - Genomics 2020Quote: ... After cleanup with the SPRIselect reagent kit (Beckman Coulter), the libraries were constructed by performing the following steps ...
-
bioRxiv - Genomics 2020Quote: ... and the SPRIselect Reagent Kit (Beckman Coulter, Indianapolis, IN). Next ...
-
bioRxiv - Genomics 2019Quote: ... and the SPRIselect Reagent kit (Beckman Coulter, Indianapolis, IN). Next ...
-
bioRxiv - Microbiology 2021Quote: ... purified using the Agencourt AMPureXP kit (Beckman Coulter Genomics), and sequenced on an Illumina NextSeq 500 system (60 bp read length ...
-
Proteolytic cleavage of the extracellular domain affects signaling of parathyroid hormone receptor 1bioRxiv - Pharmacology and Toxicology 2022Quote: ... cAMP was measured using a RIA kit (Beckman Coulter), and inositol phosphates were separated by chromatographic separation of myo-[2-3H]inositol phosphates as described previously (29) ...
-
bioRxiv - Microbiology 2022Quote: ... along with the Agencourt RNAdvance Tissue Kit (Beckman Coulter) and a KingFisher™ Flex Purification System (Thermo Fisher Scientific) ...
-
bioRxiv - Cell Biology 2022Quote: ... After a cleanup with SPRIselect Reagent Kit (Beckman, C10640), final libraries were quantified using a Qubit™ 1X dsDNA HS Assay Kit (Invitrogen ...
-
bioRxiv - Molecular Biology 2020Quote: ... and purified using Agencourt RNAClean XP Kit (Beckman Coulter). The rRNA depleted cytoplasmic RNA and nuclear RNA were then treated with RNaseIII according to Ion Total RNA-Seq protocol v2 and purified with Magnetic Bead Cleanup Module (Thermo Fisher) ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... using the Agencourt DNAdvance Magnetic Bead Kit (Beckman Coulter) or the MagAttract HMW DNA Kit (Qiagen) ...
-
bioRxiv - Microbiology 2022Quote: ... Amplified DNA was cleaned using a commercial kit (Beckman Coulter Agencourt AMPure XP ...
-
bioRxiv - Physiology 2023Quote: ... Commercial radioimmunoassay kits (M/s Beckman Coulter IM 1188) with analytical sensitivity of 0.03 ng/mL and including a highly specific progesterone antibody were used for progesterone estimation ...
-
bioRxiv - Neuroscience 2023Quote: ... Size selection with SPRIselect Reagent Kit (Beckman Coulter, B23318) was used to separate amplified cDNA molecules for 3’ gene expression and cell surface protein construction ...
-
Short-range interactions between fibrocytes and CD8+ T cells in COPD bronchial inflammatory responsebioRxiv - Pathology 2023Quote: ... permeabilized using the IntraPrep Permeabilization Reagent Kit (Beckman Coulter) and stained with anti-CD107a-PE or isotype control ...
-
Short-range interactions between fibrocytes and CD8+ T cells in COPD bronchial inflammatory responsebioRxiv - Pathology 2023Quote: ... permeabilized using the IntraPrep Permeabilization Reagent Kit (Beckman Coulter) and stained with anti-Granzyme-APC ...
-
bioRxiv - Synthetic Biology 2024Quote: ... kit and Agencourt AMPure XP beads (Beckman Coulter, USA), respectively ...
-
bioRxiv - Immunology 2021Quote: ... Env+ fraction and spike density were determined by single vesicle flow cytometry (113, 114), using a commercial kit (vFCTM Assay kit, Cellarcus Biosciences, La Jolla, CA) and flow cytometer (CytoFlexS, Beckman Coulter, Indianapolis, IN). Briefly ...
-
bioRxiv - Neuroscience 2021Quote: ... 4 × 5-s bursts) and subsequently centrifuged at 48,000g at 4°C (Beckman Avanti J-251 Ultracentrifuge; Beckman Coulter, Fullerton, CA) for 30 min ...
-
bioRxiv - Biophysics 2021Quote: ... transferred to 15 mL conical tube and centrifuged at 233 x g for 5 min at 22 °C using a swinging bucket rotor (Beckman Coulter). Pelleted cells were resuspended and washed once with 1x PBS (-CaCl2 ...
-
bioRxiv - Cell Biology 2021Quote: ... OptiPrep top to bottom density gradients 5 % to 40 % were performed as described66 in 12mL open top polyallomer tubes (Beckman Coulter). 1mL fractions were collected from the top ...
-
bioRxiv - Biophysics 2021Quote: ... and the fractions containing the protein of interest were combined and concentrated to 2mL at a final concentration of 3.85 mg/mL by repeated centrifugation in an Amicon Ultra Centrifugal Filter at 4k rpm for 5 min at 4 °C (Beckman SX4250). The concentrated protein was incubated overnight with TEV (1:50 mass ratio ...
-
bioRxiv - Plant Biology 2022Quote: ... diluted four times with 5 mM HEPES-KOH pH = 7.5 and centrifuged at 21,5000 rpm for 20 min (Beckman Coulter JA 25.50).
-
bioRxiv - Neuroscience 2020Quote: ... A sucrose density gradient was prepared with 5 ml of both 0.8 M and 1.2 M sucrose in thin wall ultracentrifugation tubes (Beckman Coulter, Cat # 344059). P2 homogenate was mixed with 1 ml of homogenizing buffer ...
-
bioRxiv - Microbiology 2022Quote: ... Pellets were resuspended in 5 ml SM buffer and purified via CsCl density gradient ultracentrifugation (Optima XPN-80 ultracentrifuge; Beckman Coulter) at 19,200xg for 18 h at 10°C ...
-
bioRxiv - Cell Biology 2019Quote: ... with 5% fetal bovine serum (FBS) and measured on flow cytometry with well-established compensated matrix (CytoFLEX, Beckman Coulter, Pasedena, CA). Analyses were performed using CytExpert Software (Beckman Coulter).
-
bioRxiv - Molecular Biology 2020Quote: ... Samples were brought to a final volume of 300 μl if necessary with lysis buffer and loaded on a non-linear sucrose gradient prepared by layering different percentage of sucrose solutions (5%, 34% and 55%) into an Open-Top Thinwall Ultra-Clear Tube (Beckman, 344059). A 5x gradient buffer (100 mM HEPES pH 7.6 ...
-
bioRxiv - Biochemistry 2020Quote: ... cells were centrifuged at 4 ℃ for 5 minutes at 300g and suspended in HBSS and analyzed with a flow cytometer (CytoFLEX, Beckman Coulter). Mean fluorescence intensity was calculated for each group.
-
bioRxiv - Immunology 2019Quote: ... CECs were pre-incubated with human recombinant CCL5 (1-1000 ng/ml, R&D) and/or CD35 blocking antibody (5-20 μg/ml, clone J3D3, Beckman Coulter) for an hour prior to infection.
-
bioRxiv - Biochemistry 2021Quote: ... live cells were stained with Alexa 647-conjugated M1 anti-FLAG antibody (1:1000, 5 minutes) and analyzed on a Cytoflex flow cytometer (Beckman Coulter).
-
bioRxiv - Immunology 2021Quote: ... Insoluble proteins and cell debris were removed by centrifugation (13,000 rpm, 30 minutes at 5°C, JA-30.50Ti rotor, Beckman, Palo Alto, CA). The soluble CuMVTT CP protein in clarified lysate was pelleted using saturated ammonium sulfate (1:1 ...
-
Preferred endocytosis of amyloid precursor protein from cholesterol-enriched lipid raft microdomainsbioRxiv - Neuroscience 2020Quote: ... 1.6 ml of 35% (w/v) sucrose and 5% (w/v) sucrose in MBS were layered in a 5.1 ml ultracentrifuge tube (Beckman Coulter, #326819) to form a discontinuous sucrose gradient ...
-
bioRxiv - Cell Biology 2022Quote: ... After ultracentrifugation at 200,000 x g for 5 h at 4°C (SW55Ti rotor in a XL-90 Beckman Coulter centrifuge), fractions containing purified EVs or purified phages were extracted from the top following the scheme in Supp ...
-
bioRxiv - Molecular Biology 2022Quote: ... The gradients were prepared using freeze-thaw method42 in Thinwall Ultra-Clear ultracentrifuge tubes (5 ml, 13×51 mm; Beckman-Coulter). To create the 50% sucrose cushion ...
-
bioRxiv - Microbiology 2022Quote: The purified AdgI/AdgH complex sample was diluted to 0.5 mg/ml using the sample buffer containing 25 mM Tris-HCl pH 7.5 and 150 mM NaCl and subjected to the sedimentation velocity measurements using an Optima XL-I analytical ultracentrifuge (Beckman-Coulter). Data was analyzed with the SEDFIT and SEDPHAT programs33,34.
-
bioRxiv - Immunology 2022Quote: ... Cells were pelleted at 400xg for 5 min and washed with PBS twice before flow cytometry (Beckman Coulter CytoFLEX Flow Cytometer). Analysis of phagocytic ability was undertaken using FCS Express.
-
bioRxiv - Cell Biology 2022Quote: ... and the pellet was resuspended with 5 ml buffer B and transferred into a 14 ml ultracentrifuge tube (331372, Beckman Coulter). 9 ml Buffer C (1.8 M sucrose ...
-
bioRxiv - Molecular Biology 2022Quote: Nuclei were isolated from 200 cotyledons of 5-day-old seedlings and purified using a two-layer Percoll gradient at 3000 g before staining with 0.5 μM DAPI and sorting by FACS according to their ploidy levels using a MoFlo Astrios EQ Cell Sorter (Beckman Culture) in PuraFlow sheath fluid (Beckman Coulter ...
-
bioRxiv - Genetics 2023Quote: ... STR DNA fragments were amplified using a pair of specific primers with 5’ terminal parts of P5 and P7 Illumina adapters (Table S1) and purified by 100% (v/v) SPRIselect beads (Beckman Coulter). In the second step ...
-
bioRxiv - Immunology 2023Quote: ... The live singlets were counted using DAPI 1:10 dilution by mixing 45 μl of cell suspension with 5 μl DAPI by gating on FSC-A vs FSC-H measuring average event/ μl on Cytoflex (Beckman Coulter). Subsequently ...
-
bioRxiv - Plant Biology 2023Quote: ... for 15 minutes at 20°C followed by 5 minutes at 65°C for inactivation of the enzymes and purified with 1X AMPure XP beads (Beckman Coulter); 1D2 Adapters (ONT ...
-
bioRxiv - Cell Biology 2023Quote: ... ice-cold 5 mL 2.5 M sucrose was added to the bottom of an open-top plastic tube (Beckman-Coulter 344058), carefully topped up with ice-cold 5 mL 1.875 M sucrose ...
-
bioRxiv - Neuroscience 2024Quote: ... were loaded onto 10–50% continuous sucrose density gradients in polysome buffer (20 mM HEPES pH 7.4, 150 mM KCl, 5 mM MgCl2) (Gradient Master™, BioComp) and centrifuged in an SW40Ti swing-out rotor (Beckman Coulter) at 250,000 g for 2 h at 4°C ...
-
bioRxiv - Biochemistry 2024Quote: ... Supernatant was incubated in a boiling water bath for 5 min and centrifuged for 10 min at 40,000 rpm (Beckman Ti45 rotor). Supernatant was supplemented with ammonium sulfate (1 g per 6.1 ml) ...
-
bioRxiv - Microbiology 2024Quote: ... incubated at 25°C and 5% CO2 (PF) or 37°C and 5% CO2 (BF) for 60 min and analyzed using a CytoFLEX flow cytometer (Beckman Coulter). Events (10,000 ...
-
bioRxiv - Microbiology 2024Quote: ... 0.2% Triton X-100 and 100 µg/mL chloramphenicol) and ultracentrifuged at 42500 rpm for 5 h in type 70 Ti rotor (Beckman Coulter) at 4°C ...
-
bioRxiv - Neuroscience 2022Quote: ... pooled and purified with Agencourt AMPure XP kit (Beckman Coulter). Purified PCR products were quantified using a Qubit dsDNA HS assay kit (Life Technologies ...
-
bioRxiv - Microbiology 2019Quote: ... and Agencourt® RNAClean® XP Kit (Beckman Coulter, USA). Measurement of RNA yield and quality was performed using Qubit® RNA HS assay kit (Thermo Fisher ...
-
bioRxiv - Microbiology 2020Quote: ... and Agencourt® RNAClean® XP Kit (Beckman Coulter, USA). Measurement of RNA yield and quality was performed using Qubit® RNA HS assay kit (Thermo Fisher ...
-
bioRxiv - Microbiology 2021Quote: DNA was extracted using the GenFind v2 kit (Beckman Coulter) and libraries prepared using the NexteraFlex kit (Illumina Inc. ...
-
bioRxiv - Microbiology 2022Quote: ... and the Agencourt RNAdvance Tissue Kit (Beckman Coulter, Krefeld, Germany). FFPE sections were loaded into a microTUBE-130 Screw-Cap (Covaris ...