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Citations for Beckman :
501 - 550 of 569 citations for Parvalbumin PVALB cDNA ORF Clone Mouse N His tag since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: ... cDNA samples were fragmented and selected for a target size via double-sized magnetic bead selection (Beckman-Coulter SPRIselect). Samples were then ligated with adaptor oligos and PCR-amplified with dual library indices for sample demultiplexing ...
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bioRxiv - Immunology 2023Quote: ... The cDNA libraries were purified after each enzymatic reaction using the AMPure XP beads (Beckman Coulter, A63882, Brea, US). All the samples were loaded on a SpotON flow cell (Oxford Nanopore Technologies ...
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bioRxiv - Immunology 2023Quote: ... Thereafter a cDNA cleanup was performed using 0.6x and 0.8x volumes of Agencourt AMPure XP SPRI beads (Beckman Coulter) which was then quantified using a Qubit dsDNA HS Assay Kit (Life Technologies) ...
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bioRxiv - Genetics 2023Quote: ... The cDNA and library were purified as indicated in the manufacturer’s protocol with AMPure XP beads (Beckman Coulter #A63881). After the library amplification and purification ...
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bioRxiv - Microbiology 2024Quote: ... using the oligo(dT) adapter and RNA–cDNA hybrids were purified using Agencourt RNAClean XP magnetic beads (Beckman Coulter). The sequencing adapter was ligated to the mRNA using the T4 DNA ligase (New England Biolabs ...
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bioRxiv - Immunology 2024Quote: ... Thereafter a cDNA cleanup was performed using 0.6x and 0.8x volumes of Agencourt AMPure XP SPRI beads (Beckman Coulter) which was then quantified using a Qubit dsDNA HS Assay Kit (Life Technologies) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... cDNA was purified using a 1.8x bead to sample volume of AMPure XP beads (Beckman Coulter cat. no. A63882) and used in a junction PCR to amplify only plasmid-derived mRNA with primers that span a synthetic intron of the pSTARR-seq_fly reporter vector ...
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bioRxiv - Cancer Biology 2022Quote: ... The cell pellet was disaggregated and incubated with 2 μL of anti-pan cytokeratin-FITC (Beckman Coulter, Cat. No. IM2356U, Clone J1B3). A separate tube containing cells unstained for surface E-Cadherin was incubated after permeabilization with E-Cadherin antibody- (R&D Cat ...
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bioRxiv - Immunology 2023Quote: ... frozen splenocytes were stained as described above using surface antibodies against following TCR Vβ segments (clone, brand, fluorophore): VB3 (CH92, Beckman Coulter, FITC), VB4 (WJF24 ...
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bioRxiv - Immunology 2021Quote: ... including PE-Cy7-conjugated mouse anti-human CD45 (Beckman Coulter, CA, USA), efluor660-conjugated rat anti-human OCT3/4 (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... Corresponding isotype controls were as follows: IgG1 Mouse-PE (A0779, Beckman Coulter), IgG2a Mouse-PE (A09142 ...
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bioRxiv - Genetics 2024Quote: DNA was extracted from mouse spleen tissue using DNAdvance kit (Beckman Coulter). Multiplexed sequencing libraries were prepared using the Twist 96-Plex Library Prep kit (TWIST Bioscience) ...
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bioRxiv - Cancer Biology 2020Quote: The barcode split of the cDNA amplification reaction (from 10x Single Cell 3’ v3 Step 2.2) was purified via 1.2x SPRI Select (Beckman Coulter #B23317). cDNA products were eluted in 40 µL of EB ...
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bioRxiv - Immunology 2021Quote: ... Full-length double-stranded cDNA was purified with 30 μl (0.6x) of AMPure XP magnetic beads (Beckman Coulter, Cat. #A63881) and eluted in 20 μl of water ...
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bioRxiv - Microbiology 2020Quote: ... and the resulting cDNA was PCR amplified (11 cycles) and purified using the Agencourt AMPure XP kit (Beckman Coulter Genomics). For Illumina NextSeq sequencing ...
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bioRxiv - Genomics 2021Quote: ... The cDNA was purified using RNA XP beads purification with 1:1.8x ratio of sample to beads (Beckman Coulter, A63987) and eluted in 24.5 µl water ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... and cDNA fragments (150 ∼ 200 bp in length) were then purified using the AMPure XP System (Beckman Coulter, Beverly, USA). Paired-end sequencing of libraries (PE150 ...
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bioRxiv - Immunology 2020Quote: ... Twenty-five percent of the cDNA volume was subjected to fragmentation and double-sided SPRIselect (Beckman Coulter, Indianapolis, IN, USA) was used for PCR purification and clean-up ...
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bioRxiv - Genomics 2021Quote: ... and after cDNA amplification separated the sample as follows: We performed a 0.6X left-sided cleanup reaction with SPRIselect (Beckman Coulter) and collected both the beads (which carry material for preparing the gene expression library ...
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bioRxiv - Microbiology 2022Quote: ... The cDNA was then amplified using a high fidelity DNA polymerase and purified with Agencourt AMPure XP beads (Beckman Coulter). The cDNA samples were pooled in Equimolar amounts and the rDNA within the pool was depleted targeting 24 unique Sequences (Supplementary Table S1 ...
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bioRxiv - Immunology 2024Quote: ... Size-selection and purification of cDNA fragments with preferentially 200 bp in insert length was performed using SPRIBeads (Beckman-Coulter). Size distribution of cDNA libraries was measured using the Agilent high sensitivity DNA assay on a Tapestation 2200 (Agilent) ...
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bioRxiv - Immunology 2023Quote: ... the ADT- and HTO-containing supernatant fraction was separated from the cDNA fraction derived from cellular mRNAs using 0.6X SPRI beads (Beckman Coulter).
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bioRxiv - Pathology 2023Quote: ... Ligated cDNAs were amplified following 15 PCR cycles and PCR products were purified using AMPure XP Beads (Beckman Coulter Genomics). Libraries were validated using a Bioanalyzer on a DNA1000 chip (Agilent ...
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bioRxiv - Neuroscience 2024Quote: ... Full-length double-stranded cDNA was purified with 30 μL (0.6x) of AMPure XP magnetic beads (Beckman Coulter, Cat.#A63881) and eluted in 20 μL of water ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Plates were scanned for absorption at 600 nm and growing clones were identified using custom software and picked and duplicated by a Biomek FXp (Beckman Coulter, Krefeld, Germany) liquid handling system.
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bioRxiv - Immunology 2021Quote: ... 4 ng of RNA were retrotranscribed and cDNA was amplified using 13 cycles and purified with AMPure XP beads (Beckman Coulter). Concentration was determined using Qubit 3.0 (Life Technologies ...
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bioRxiv - Microbiology 2022Quote: ... Samples were incubated for 2 h at 16°C and double stranded cDNA was purified using AMPure XP beads (Beckman Coulter). For testing transcription activity of predicted genes ...
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bioRxiv - Cell Biology 2020Quote: ... Then first strand synthesis and second strand synthesis were performed and the double stranded cDNA was purified (AMPure XP, Beckman Coulter). The cDNA was end repaired ...
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bioRxiv - Immunology 2021Quote: ... 0.1 ng cDNA was used per sample and the libraries were cleaned up with 1.8x AMPure® XP beads (Beckman Coulter). Quality and integrity of nucleic acids were assessed using the Agilent Technologies 2100 Bioanalyzer (Agilent Technologies ...
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bioRxiv - Immunology 2021Quote: ... Fragmented and A-tailed cDNA was purified by performing a dual-sided size-selection using SPRIselect magnetic beads (Beckman Coulter #22667). A partial TruSeq Read 2 primer sequence was then ligated to the fragmented and A-tailed end of cDNA molecules via an incubation of 20°C for 15 minutes on a C1000 Touch Thermal Cycler ...
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bioRxiv - Immunology 2021Quote: ... Fragmented and A-tailed cDNA was purified by performing a dual-sided size-selection using SPRIselect magnetic beads (Beckman Coulter #22667). A partial TruSeq Read 2 primer sequence was ligated to the fragmented and A-tailed end of cDNA molecules via an incubation of 20°C for 15 minutes on a C1000 Touch Thermal Cycler ...
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bioRxiv - Immunology 2021Quote: ... Amplified cDNA was purified and separated from amplified HTOs using a 0.6x size selection via SPRIselect magnetic bead (Beckman Coulter #22667) and a 1:10 dilution of the resulting cDNA was run on a Fragment Analyzer (Agilent Technologies #5067-4626 ...
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bioRxiv - Genomics 2020Quote: ... Then first strand synthesis and second strand synthesis were performed and the double stranded cDNA was purified (AMPure XP, Beckman Coulter). The cDNA was end repaired ...
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bioRxiv - Immunology 2022Quote: ... Amplified cDNA was purified and separated from amplified HTOs using a 0.6x size selection via SPRIselect magnetic bead (Beckman Coulter #22667) and a 1:10 dilution of the resulting cDNA was run on a Fragment Analyzer (Agilent Technologies #5067-4626 ...
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bioRxiv - Immunology 2022Quote: ... Fragmented and A-tailed cDNA was purified by performing a dual-sided size-selection using SPRIselect magnetic beads (Beckman Coulter #22667). A partial TruSeq Read 2 primer sequence was ligated to the fragmented and A-tailed end of cDNA molecules via an incubation of 20°C for 15 minutes on a C1000 Touch Thermal Cycler ...
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bioRxiv - Physiology 2022Quote: ... cDNA fragments of 150∼200 bp were selected by purification of library fragments using AMPure XP system (Beckman Coulter, Beverly,USA). PCR was performed with Phusion High-Fidelity DNA polymerase ...
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bioRxiv - Molecular Biology 2024Quote: ... PCR amplification was then performed using the SMART PCR primer (AAGCAGTGGTATCAACGCAGAGT) and cDNA was subsequently purified using AMPure beads (Beckman Coulter). The library was split and a further 20 or 25 PCR cycles were performed using a biotin oligonucleotide (5—PCBioTACACGACGCTCTTCCGATCT ...
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bioRxiv - Developmental Biology 2023Quote: ... the tagmented cDNAs were amplified using a unique combination of i5 and i7 indexes and purified using AMPure XP beads (Beckman Coulter). To control for the appropriate size distribution of 250-1000bp of the libraries a high sensitivity bioanalyzer (Agilent Technologies ...
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bioRxiv - Cancer Biology 2023Quote: ... MULTI-seq barcode fragments were separated from endogenous cDNA fragments during the first round of size selection using SPRIselect beads (Beckman coulter). Next ...
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bioRxiv - Genomics 2023Quote: ... PCR amplification was then performed using the SMART PCR primer (AAGCAGTGGTATCAACGCAGAGT) and cDNA was subsequently purified using AMPure beads (Beckman Coulter). The library was split and a further 20 or 25 PCR cycles were performed using a biotin oligonucleotide (5—PCBio-TACACGACGCTCTTCCGATCT ...
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bioRxiv - Cancer Biology 2024Quote: ... Each 384 well plate was then pooled into a 1 μL reaction product and cDNA was purified using 0.8X volume of AMPure XP beads (Beckman Coulter, A63882). Each pool was analyzed for concentration and size distribution using Bioanalyzer (Agilent).
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bioRxiv - Immunology 2020Quote: ... Amplified cDNA went through two subsequent rounds of cleaning by using Agencourt AMPure XP beads (Beckman Coulter UK Ltd, High Wycombe, UK) at a 1.0x ratio on a liquid handler Zephyr G3 NGS Workstation (PerkinElmer ...
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bioRxiv - Neuroscience 2020Quote: ... Amplified cDNA samples were then cleaned up of short fragments with AMPure XP beads (1:1 ratio; Beckman Coulter, Brea, CA, USA) and checked for cDNA quality and concentration on a 2100 Bioanalyzer (Agilent Technologies ...
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bioRxiv - Immunology 2022Quote: ... Smaller feature barcode fragments were separated from longer amplified cDNA using a 0.6X cleanup with aMPure XP beads (Beckman Coulter catalog # A63882). Libraries were constructed using the Chromium Single Cell 5’ Feature Barcode Library Kit (10X Genomics PN 1000080 ...
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bioRxiv - Physiology 2022Quote: ... The amplified cDNA (a total of 30-50 μL) was purified using 90 μL Beckman Coulter AMPure XP beads (Beckman Coulter, #A63880) following the manufacturer’s guideline ...
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bioRxiv - Microbiology 2024Quote: ... the amplified cDNA was purified with a double-sided clean-up step (right: 0.5x, left: 1.5x; RNAClean XP beads, Beckman Coulter, Brea, CA, USA), followed by a left-sided clean-up step (0.7x) ...
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bioRxiv - Cancer Biology 2024Quote: ... cDNA fragments of 150-200bp length were purified to a concentration of 1.5ng/μl using AMPure XP beads (Beckman Coulter, Beverly, USA). Assessment of library effective concentration and RNA quality were performed using Agilent 2100 Bioanalyzer and Qubit2.0 (Thermo Scientific ...
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bioRxiv - Microbiology 2023Quote: ... The cDNA was purified between enzymatic reactions and the size selection of the library performed with AMPure SpriSelect Beads (Beckman Coulter Genomics). The titer and size of the libraries was assessed on the LabChip GX (Perkin Elmer ...
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bioRxiv - Biochemistry 2024Quote: ... Double-stranded cDNA duplexes were purified from the 21 µL reaction by the addition of 63 µL of AMPure XP (Beckman Coulter, A63880) and incubation at room temperature for 10 minutes ...
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bioRxiv - Cancer Biology 2024Quote: ... Smaller feature barcode fragments were separated from longer amplified cDNA using a 0.6X cleanup with aMPure XP beads (Beckman Coulter catalog # A63882). Libraries were constructed using the 3’ Feature Barcode Kit (10X Genomics PN 1000276 ...