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Citations for Beckman :
501 - 550 of 582 citations for Mouse Anti Dengue Virus Serotype 2 NS1 Antibody CM474 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... and anti-HIV-1 p24 clone KC57-RD1 (Beckman Coulter, CA, USA). The secondary goat anti-mouse and anti-rabbit Abs conjugated to Alexa488 ...
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bioRxiv - Microbiology 2020Quote: ... staining with anti-HIV-1 Core Antigen Clone KC57 FITC (Beckman Coulter) and detection of HIV-1-infected cells by flow cytometry ...
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bioRxiv - Cancer Biology 2019Quote: ... - anti-MIR300 and -TUG1shRNA oligonucleotides (ODNs) (Beckman Research Institute, City of Hope), 1.25 μg/ml anti-TGFβ neutralizing antibody (1D11 ...
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bioRxiv - Immunology 2023Quote: ... Cells were then stained with anti-CD19-PC5.5 (J3-119, Beckman Coulter), Live/Dead Aqua (Invitrogen ...
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bioRxiv - Cancer Biology 2020Quote: ... and NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 1 E7335S, Index Primers Set 2, E7500S) with Agencourt AMPure XP magnetic beads (Beckman coulter A63881) exactly as per manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... and extracellular vesicles including exosomes and virions were pelleted by ultracentrifugation at 141,000 g at 4 °C for 2 h in a swinging-bucket rotor (SW28; Beckman Coulter, Brea, CA, USA). The pellet was resuspended in filtered PBS and centrifuged at 18,300 g and 4°C for 10 min to remove insoluble aggregates ...
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bioRxiv - Microbiology 2020Quote: ... The clarified supernatant was overlaid onto a 20% (w/v) sucrose cushion in Tris-NaCl-EDTA buffer and centrifuged at 134,000 g for 2 hours (Beckman Coulter 30 Ti rotor). The resulting viral pellet was suspended in VCM by repeat pipetting and aliquots stored in cryovials at minus 70°C until needed ...
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bioRxiv - Genomics 2020Quote: ... were layered onto a 1.8M sucrose cushion and ultra-centrifuged at 24,400 rpm at 4°C for 2 hours using a SW28 swinging bucket rotor (Beckman Coulter Optimal centrifuge #L90K). The nuclear pellet was re-suspended in 2% BSA in 1X PBS ...
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bioRxiv - Microbiology 2020Quote: ... EMVs were obtained by ultracentrifugation from 4 mL of filtrate at 100,000 × g (average centrifugal force) and 4°C for 2 h with an OptimaX centrifuge (Beckman Coulter, Brea, CA, USA). The pellets were resuspended in 400 μL of Dulbecco’s phosphate-buffered saline (DPBS ...
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bioRxiv - Neuroscience 2022Quote: ... The tubes were sealed and centrifuged at 50,000 rpm at 4°C for 2 h in an OptimaTM L-90K ultracentrifuge using the 70.1Ti rotor (Beckman Coulter, Brea, CA, USA). After the centrifugation ...
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bioRxiv - Cell Biology 2020Quote: ... 3 μM or 10 μM from a 2 mM or 10 mM stock solution using a Biomek FX with 384-well head (Beckman Coulter, Indianapolis, IN). The DMSO concentration was kept below or at 0.5% v/v ...
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bioRxiv - Physiology 2022Quote: ... the library preparation step followed the CEL-Seq 2 protocol85: the RT product was digested by ExoI and HinFI and purified using AMpure XP beads (Beckman Coulter™, USA). Then ...
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bioRxiv - Microbiology 2023Quote: ... then the colonies were diluted and inoculated into the Neg MIC EN-2J panel containing β-lactams listed in Table 2 and incubated in MicroScan Walkway40 Plus (Beckman Coulter Inc.). Breakpoint criteria for susceptible (S) ...
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bioRxiv - Biochemistry 2020Quote: Sedimentation velocity experiments were performed using a ProteomeLab XL-1 analytical ultracentrifuge with the use of an AN60-Ti rotor and Epon-2 channel centerpieces (Beckman Coulter, Brea, CA, USA). Protein samples were diluted with SANS buffer to concentrations yielding an absorbance of 0.5 at 280 nm as measured with an 8454 UV–Vis spectrophotometer (Agilent ...
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bioRxiv - Microbiology 2022Quote: ... The success of the amplifications was checked in a 2% agarose gel and PCR products were subsequently purified using Agencourt AMPure XP magnetic beads kit (Beckman Coulter, Brea, CA, USA), quantified with the QuantiFluor™ dsDNA System (Promega ...
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bioRxiv - Molecular Biology 2019Quote: ... The concentrated urine sample was resuspended in 3.2 ml of a 50% iodixanol solution and layered on the bottom of a 17 ml Thinwall Polypropylene Tube (Beckman Coulter, Fullerton, California, USA). A discontinuous DG was prepared by additional layering of 4 ml of 20% ...
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bioRxiv - Neuroscience 2019Quote: ... and 1□mM DTT) before a centrifugation process of 2 h 20 min at 30,000□× g (Beckman J-25 centrifuge with a J13.1 rotor). The supernatant was carefully aspirated and the extracted nuclei in a form of a pellet were resuspended in a nuclear storage buffer (15% sucrose ...
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bioRxiv - Biochemistry 2019Quote: ... and 5 mM MgCl2 at pD 7.5 corresponding to a pH reading of 7.1) at 25°C as follows: spin column at 2000 rpm for 2 min in a tabletop centrifuge (Beckman Coulter Allegra R Tabletop Centrifuge), discard the flow-through ...
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bioRxiv - Microbiology 2020Quote: ... and layered on top of a two-step sucrose gradient (20%/50% w/v in HNE) and centrifuged for 2 h at 154 000 x g at 4 °C in a SW41 rotor (Beckman Coulter, Woerden, the Netherlands) to clear from cell debris ...
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bioRxiv - Microbiology 2023Quote: The amplicons obtained with each of the strategies (using pools 1 and 2) were pooled in each group and purified with one volume of AMPure XP beads (Beckman Coulter, Brea, CA, USA). The beads were washed with 80% ethanol ...
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bioRxiv - Biochemistry 2023Quote: ... cells were treated with the drugs (20 µM to a suspension at 500,000 cells/mL in PBS containing 2 % FBS at 37°C) and directly analyzed by flow cytometry (Gallios, Beckman-Coulter, 405/488 nm laser) over 2 h ...
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bioRxiv - Biochemistry 2023Quote: ... using an analytical ultracentrifuge XL-I with an Anti-60 rotor (Beckman Coulter) and double-sector cells (Nanolytics ...
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bioRxiv - Microbiology 2019Quote: ... Intracellular Gag staining was carried out using a Gag monoclonal antibody conjugated to Phycoerythrin (KC-57 RD1 Beckman Coulter). 1×105 cells from a HIV GPV-zip coded library were washed once with FACS buffer and fixed with 100 µl of BD cytofix for 10 minutes at room temperature in the dark ...
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bioRxiv - Immunology 2022Quote: ... The cells were stained with a panel of monoclonal antibodies containing: ECD CD16 clone 3G8 (Beckman Coulter; cat.no. A33098), BV711 CD45 clone HI30 (BD Biosciences ...
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Nonsense Mediated RNA Decay Is a Unique Vulnerability of Cancer Cells with SF3B1 and U2AF1 MutationsbioRxiv - Cell Biology 2021Quote: ... and then stained for flow cytometry using the CD34-phycoerythrin (PE) antibody (PE-pool, Beckman Coulter, IM1459U, Brea, CA). RNA-sequencing libraries were then prepared from RNA isolated from the bulk mutant AML bone marrow cells and the normal CD34+ bone marrow cells using TruSeq Stranded Total RNA kit (Illumina ...
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bioRxiv - Cell Biology 2022Quote: ... unbound PKH67 dye and unbound antibodies by overnight bottom-up sucrose density gradient (SDG) ultracentrifugation at 192,000 g (SW40 rotor Beckman Coulter ...
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bioRxiv - Immunology 2022Quote: The TCR V beta repertoire kit contained antibodies to over 76% of V βeta families (Beckman Coulter, Fullerton, CA). The cells were stained with these antibodies in combination with tetramers or dextramers for 20 minutes at RT to determine the TRBV repertoire of antigen specific cells ...
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bioRxiv - Cancer Biology 2024Quote: ... Platelet concentration was obtained by flow cytometry using an antibody against human CD41 labelled with PC7 (6607115, Beckman Coulter) and MP-count beads (6607115 ...
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bioRxiv - Cancer Biology 2020Quote: ... Filtered media containing lentiviral particles were centrifuged at 24000rpm at 20°C for 2 hrs in 90Ti rotor in ultra-centrifuge (Beckman Coulter Optima TM L-100K). Viral particle pellet was obtained after discarding supernatant post centrifugation and suspended in 100 μl OptiMEM media for overnight at 4°C.The collected lentivirus suspension was used for transfection or stored for later use at −80°C ...
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bioRxiv - Physiology 2020Quote: ... one bird per pen in Experiment 2) and centrifuged for 5 min at 17013 × g using a Beckman microfuge (Model E 348720, Beckman Instruments, INC, Palo Alto, CA). Then the viscosity of ileal supernatant was measured using a Brookfield cone-plate digital viscometer (Model LVDV-Ⅲ ...
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bioRxiv - Cancer Biology 2020Quote: ... isotype controls for intracellular stains and subjected to post-acquisition compensation using VersaComp Antibody Capture Bead Kit (Beckman Coulter #B22804). Data was analyzed using Kaluza Software (Beckman Coulter) ...
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bioRxiv - Immunology 2021Quote: ... Cells were stained with following antibody mix for 30 minutes on ice: pan-γδTCR-PE (1:10; IMMU510; Beckman Coultier) and CD69-APC (1:25 ...
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bioRxiv - Immunology 2023Quote: Human TCR Vβ repertoire analysis was performed using antibodies against the most frequent TCR Vβ variants (covering about 70% normal human TCR Vβ repertoire, as reported by Beckman Coulter “Beta Mark TCR Vbeta Repertoire Kit” (cat.nr. ...
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bioRxiv - Microbiology 2020Quote: ... The dyes used were: KC57 anti-HIV-1 core antigen-PE (Beckman coulter CO604667), Live/Dead fixable Far Red Dead Cell Stain Kit (Invitrogen L10120 ...
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bioRxiv - Genomics 2022Quote: ... HIV-1 p24 was stained with Anti-HIV-1 p24-PE (KC57) (Beckman Coulter) for 30 min on ice and washed again 3 times before measuring on the BD FACSCelesta™.
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bioRxiv - Immunology 2022Quote: ... Human Vγ9Vδ2 T lymphocytes were phenotyped using PE-labeled anti-NKG2A (#Z199, Beckman Coulter), PE-labeled anti-NKG2D (#ON72 ...
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bioRxiv - Immunology 2023Quote: ... Cells were washed and stained with anti-KIR2DL1/S1 mAb (EB6-APC, Beckman Coulter) for 30 mins at 4°C ...
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bioRxiv - Biochemistry 2019Quote: ... Resuspended cells were lysed by French Press (8,000 psi, 4°C, 2 passages) and lysates were centrifuged (Beckman 60Ti rotor, 30 min, 50,000 rpm, 4°C). Pellets were resuspended in 100 ml of buffer B (PBS [140 mM NaCl ...
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bioRxiv - Microbiology 2023Quote: ... another 2 mL of staining bacterial samples was analyzed with a flow cytometry to check cell viability (Beckman CytoFLEX FCM, USA, Channel FL2-A: PE-A).
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bioRxiv - Developmental Biology 2020Quote: ... incubated with fluorophore-conjugated antibodies for 30min (see Supplementary Table 1) and FACS-sorted using MoFlo Legacy or Astrios cell sorters (Beckman Coulter). Cells were sequentially gated based on size and granularity (forward scatter ...
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bioRxiv - Immunology 2022Quote: ... Immunofluorescence-labelled cells were washed twice in PBS-EDTA after incubation with the antibody and then subjected to flow-cytometry analysis using a Gallios flow cytometer (Beckman Coulter). Cytotoxicity of the compounds was assessed using the MTT assay (Roche ...
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bioRxiv - Microbiology 2019Quote: ... Denmark) together with the following panel of conjugated monoclonal antibodies and analysed on a FC500 flow cytometer (Beckman Coulter, Copenhagen, Denmark): CD3-PerCP ...
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bioRxiv - Immunology 2021Quote: ... were incubated with monoclonal antibodies at 4°C for 30 min and then fixed with 4% paraformaldehyde before analysis on a Cytoflex cytometer (Beckman Coulter). Data were analyzed by Flow Jo software v.10.7 (BD Biosciences) ...
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bioRxiv - Microbiology 2020Quote: ... Whole blood staining was done with Alexa Fluor®647 labelled-VLP (2.5µg/ml) and cell subsets were discriminated using the following antibody panel: CD45 (Clone J33; Beckman Coulter, Brea, CA), CD11c (Clone BU15 ...
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bioRxiv - Immunology 2019Quote: ... CECs were pre-incubated with human recombinant CCL5 (1-1000 ng/ml, R&D) and/or CD35 blocking antibody (5-20 μg/ml, clone J3D3, Beckman Coulter) for an hour prior to infection.
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bioRxiv - Microbiology 2020Quote: ... cells were fixed with 4% FA in PBS for 90 min at room temperature and immunostained for intracellular HIV-1 CA for 30 min at 4°C using KC57-FITC antibody (Beckman Coulter) diluted in 0.1% Triton X-100 ...
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bioRxiv - Immunology 2022Quote: ... following manufacturer’s instructions with the following p24 antibodies: HIV-1 p24 clone 28B7-APC (Medimabs) and HIV-1 p24 clone KC57-PE (Beckman Coulter). Acquisition was performed on the Fortessa LSR flow cytometer (BD Bioscience ...
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bioRxiv - Cancer Biology 2024Quote: ... CD138 and CD70 for CAR-T (see table of antibodies) and tumor identification and analyzed by flow cytometry using a CytoFLEX Flow Cytometer (Beckman Coulter). Data was analyzed using FlowJo 10.10.0 software ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The FITC fluorescence signals demonstrating the antibody-receptor complex on the cell surface were quantified by flow cytometry (CytoFLEX, Beckman Coulter). Relative expression levels of GPR1 mutants were represented according to the fluorescence signals.
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bioRxiv - Immunology 2022Quote: ... washed with BD Perm/Wash buffer and stained with anti-p24-PE (KC57, Beckman Coulter) for 20 mins on ice and ...