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Citations for Beckman :
501 - 550 of 1322 citations for 2 4 Dimethyl 4' 4 methylpiperazinomethyl benzophenone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... Gradients were spun for 3 hours at 4° C and 36,000 RPM (221,632 x g) on SW41-Ti rotor (Beckman Coulter). Monosome fractions were isolated from each sucrose gradient with Brandel Density Gradient Fractionator using 1.5 mL/min flow speed and 60% CsCl ...
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bioRxiv - Cell Biology 2022Quote: ... The lysate was clarified via centrifugation (66,000 x g, 30 min, 4°C) in a Type 70 Ti rotor (Beckman). The supernatant was mixed with 1.5 ml of anti-FLAG M2 affinity gel (Sigma-Aldrich ...
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bioRxiv - Microbiology 2022Quote: ... sonicated using a sonicator (Smurt, Microtec) and ultracentrifuged at 100,000 x g for 60 minutes at 4°C (Optima XE-90, Beckman). Protein concentration of the bacterial membrane suspension was determined with Bio-Rad protein assay (BioRad).
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bioRxiv - Cell Biology 2022Quote: ... and centrifuged at 10,000g and 4°C for 30 minutes (SW41 rotor, Optima XPN-100 ultracentrifuge; Beckman Coulter, Inc.) to remove any potential large debris ...
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bioRxiv - Biochemistry 2022Quote: ... 5 mM MgCl 2 and 2 mM DTT and centrifuged at 70,000 x g for 20.5 h at 4 °C in an SW28 rotor (Beckman Coulter). Fractions corresponding to the 40S peak were collected ...
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bioRxiv - Biochemistry 2022Quote: ... Gradients were centrifuged at 42,800 x g for 15 h at 4 °C in an SW28 rotor (Beckman Coulter). Fractions of 1 mL were collected using an AKTA system (Cytiva) ...
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bioRxiv - Microbiology 2022Quote: ... at 4°C for 1.5-2h followed by 180,000 x g (Ti 70i rotor; Beckman Instruments, Inc., Fullerton, CA) at 4°C for 1.5-2h and resuspended in 1mL of phosphate buffered saline (PBS ...
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bioRxiv - Biochemistry 2022Quote: ... The crude extract was centrifuged at 200,000 g for 1 h at 4 °C (Beckman Coulter Optima LE-80K). The membrane pellet was homogenized and solubilized in 20 mM HEPES (pH 7.8 ...
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bioRxiv - Genetics 2022Quote: ... The lysate was centrifuged at 2000 rpm for 10 min at 4 °C in a JA-20 rotor (Beckman). The supernatant from the previous step was centrifuged in a Beckman Coulter high speed centrifuge equipped with a JA-20 rotor at 6000 rpm for 20 min at 4 °C ...
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bioRxiv - Neuroscience 2020Quote: ... The gradient was centrifuged at 200,000 × g for 20 h at 4°C (35,000 rpm with # Optima-XE SW41 Beckman Coulter). The gradient was collected in 2-mL fractions ...
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bioRxiv - Neuroscience 2020Quote: ... this second supernatant was centrifuged for 4 hrs at 100,000g (Optima MAX-XP ultracentrifuge, TLA-100.3 rotor, Beckman Coulter). Following ultracentrifugation ...
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bioRxiv - Plant Biology 2020Quote: ... and centrifuged at 131,500 × g for 30 min at 4 °C using a SW 32 Ti rotor (Beckman Coulter). Thylakoid membranes sedimented in 1.3 M sucrose cushion were collected and washed with 25 mM Tricine-KOH (pH 7.8) ...
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bioRxiv - Biochemistry 2022Quote: ... mixed for 10 minutes at 4°C and centrifuged at 25,000 rpm for 45 minutes (Beckman Ti-45 rotor). The supernatant was warmed to 37°C for few minutes until it turned cloudy following which it was centrifuged at 11,000 rpm at room temperature for 10 minutes (Beckman JA-20 rotor ...
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bioRxiv - Systems Biology 2020Quote: ... the 500xg supernatant from enzymatic digestion of cardiovascular tissues underwent further ultracentrifugation at 10,000xg for 10 minutes at 4°C (Beckman Coulter ...
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bioRxiv - Cell Biology 2020Quote: ... The soluble fractions released by MNase were isolated by taking supernatants after centrifugation at 13,894 rpm (16,000 rcf) at 4 °C for 30 min using SX241.5 rotor in Allegron X-30R (Beckman Coulter). The supernatants were collected and layered onto the 10-22 % linear sucrose gradient solution with buffer SG (15 mM HEPES-KOH [pH 7.4] ...
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bioRxiv - Biochemistry 2021Quote: ... Eluted fractions were pooled and subjected to a 2.5 h ultracentrifugation at 135,500 x g at 4 °C using a TLA-55 rotor (Beckman-Coulter). Finally ...
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bioRxiv - Biochemistry 2021Quote: ... Reaction mixtures were incubated for 30 minutes and centrifuged at 100,000g at 4°C for 1 hr using a TLA100 fixed angle rotor (Beckman). The supernatant fraction was carefully removed ...
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bioRxiv - Cancer Biology 2020Quote: ... Lysates were loaded on 15-50% sucrose gradient and centrifuged at 35,000 rpm for 2.5 h at 4°C in a SW41 rotor (Beckman Coulter). Polysomes were separated through a live optical density (OD ...
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bioRxiv - Molecular Biology 2020Quote: ... Ten OD260 units were loaded on a 10–50% sucrose gradient and centrifuged at 187,813 × g for 2.75 h at 4 °C in a SW41Ti rotor (Beckman Coulter). The fractions of the gradient were collected ...
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bioRxiv - Cell Biology 2021Quote: ... The crude extract was clarified by high-speed centrifugation (60 min at 36,000 ×g at 4 °C) by Beckman Coulter centrifuge.
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bioRxiv - Plant Biology 2021Quote: ... A soluble fraction was obtained by ultracentrifugation at 200,000 g for 20 minutes at 4°C on a Beckman Optima Ultracentrifuge (Beckman). Two biological replicates for SEC and IEX column separations were prepared in this manner.
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... IL-4 and IFN-γ concentrations were determined using DTX 880 Multimode Detector (Beckman Coulter, Inc., Brea, CA, USA) set to 450 nm.
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bioRxiv - Biochemistry 2021Quote: ... The gradient was centrifuged at 100,000 g for 16 hours at 4°C (rotor MLS-50, Beckman Optima MAX). Twelve fractions of 400 μL were collected from the top of the gradient and pelleted by UC at 100,000 g for 2 hours at 4°C (Figure 1) ...
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bioRxiv - Biochemistry 2021Quote: ... and 10 mM NH4Cl) and centrifuged for 16 h at 4°C with 18,000 rpm in an SW60Ti rotor (Beckman). Gradients were hand-fractionated and ribosome concentrations were determined by absorbance at 260 nm (A260 ...
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bioRxiv - Biochemistry 2021Quote: ... Samples were centrifuged at 50,000 rpm (∼166,000 × g) for 3h at 4°C using TLS-55 rotor (Beckman Coulter) in an Optima benchtop ultracentrifuge (Beckman Coulter ...
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bioRxiv - Biochemistry 2021Quote: ... Membranes were collected by ultracentrifugation at 220 000 x g for 90 min at 4°C (45Ti rotor, Beckman).
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bioRxiv - Biochemistry 2021Quote: ... and the lysate was clarified by centrifugation at 30,000 x g for 60 min at 4 °C in a JA25.50 rotor in an Avanti J-E centrifuge (Beckman). The clarified lysate was then added to 10 mL of Ni-NTA Agarose beads (Invitrogen ...
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bioRxiv - Cell Biology 2021Quote: ... The lysate was clarified by centrifugation for 30 min at 35,000 rpm at 4°C in a Type 45 Ti rotor (Beckman). Cleared lysate was incubated with 2 mL glutathione-sepharose (Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... The lysate was clarified by centrifugation for 30 min at 35,000 rpm at 4°C in a Type 45 Ti rotor (Beckman). Cleared lysate was incubated with 2 mL Ni-NTA agarose (Qiagen ...
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bioRxiv - Immunology 2022Quote: ... stained bacteria were then sonicated for 4 min (0.5 cycle, 75 A, VialTweeter) followed by determination of concentration using flow cytometry (CytoFlex; Beckman Coulter ...
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bioRxiv - Microbiology 2022Quote: ... Fractions were diluted in 5 ml PBS and washed by ultracentrifugation at 200,000 × g (60 min, 4°C, AH-650 rotor, 5 ml Beckman Ultra-Clear tubes ...
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bioRxiv - Microbiology 2022Quote: ... 100 ml cell cultures were grown over night and harvested by centrifugation at 15,000 x g for 15 min at 4 °C (Beckman JLA-16.250 rotor ...
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bioRxiv - Microbiology 2022Quote: ... 100 ml cell cultures were grown over night and harvested by centrifugation at 15,000 x g for 15 min at 4 °C (Beckman JLA-16.250 rotor, Beckman Avanti J-25, Beckman Coulter). Pellets were washed with 20 mM potassium phosphate (pH 7.5 ...
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bioRxiv - Microbiology 2022Quote: ... The supernatant fractions were collected and centrifuged at 75,600 x g for 90 min at 4 °C (Beckman JA25.50 rotor, Beckman Avanati J-25, Beckman Coulter). The resulting pellets were washed again and resuspended in 500 μl potassium phosphate buffer ...
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bioRxiv - Biochemistry 2022Quote: ... The lysate was clarified by centrifugation at 48000×g for 45 min at 4°C using a JA-25.50 rotor (Beckman), and applied on a XK 16/20 chromatography column (Cytiva ...
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bioRxiv - Biochemistry 2022Quote: ... The membrane was solubilized for 3 hours at 4°C before separation by ultracentrifugation at 100,000 g (Ti45, Beckman) for 45 min ...
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bioRxiv - Biochemistry 2022Quote: ... The elutions were combined and centrifuged at 100,000 rpm at 4°C for 40 minutes in a TLA120.2 rotor (Beckman Coulter) before resuspension of the ribosomal pellet in RNC buffer containing 1 μM ternatin-4 ...
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bioRxiv - Developmental Biology 2022Quote: ... Gradients were centrifuged at 35.000 g for 3h at 4°C in an ultracentrifuge using the SW40ti rotor head (Beckman). Fractions of 1 mL were collected from the top at 1 mL/min ...
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Reducing mitochondrial ribosomal gene expression does not alter metabolic health or lifespan in micebioRxiv - Cell Biology 2022Quote: ... and then purified using 30% Percoll gradient ultracentrifugation at 95,000g for 30 minutes at +4°C (Beckman sv41 rotor). Purified mitochondria were treated 1:200 with a protease inhibitor cocktail (Thermo Fisher Scientific HALT PI ...
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bioRxiv - Genomics 2022Quote: ... All samples were acquired on a Beckman Coulter CytoFLEX S with 4 lasers (405nm, 488nm, 561nm, 638nm) (Beckman Coulter), and operated by CytExpert Software v2.3 (Beckman Coulter ...
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bioRxiv - Neuroscience 2022Quote: ... and ultracentrifuged at 25,000 g for 20 min 4°C (or 50,000 g for 30 min 4°C) in a Beckman TLA100.3 rotor (Optima™ TLX Ultracentrifuge, Beckman). The pellet was discarded ...
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bioRxiv - Microbiology 2022Quote: ... The gradient was centrifuged at 4°C and 35,000 rpm for 2.5 h using an SW 40 Ti rotor (Beckman Coulter). Afterward ...
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bioRxiv - Microbiology 2022Quote: ... which was spun in a ultracentrifuge at 30,000 x g for 2h at 4°C (SW 60 Ti rotor, Beckman) The pellet was resuspended in 20 μl of PBS for 1 h at 4 °C ...
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bioRxiv - Neuroscience 2022Quote: ... supernatant was centrifuged for 3 h at 115,000 g and 4°C using the XPN-80 ultracentrifuge equipped with a Ti70 rotor (Beckman). EV pellets were resolved in 1 mL ice-cold PBS (Gibco ...
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bioRxiv - Microbiology 2022Quote: ... Glycoproteins in the supernatants were pelleted by centrifugation at 100,000×g at 4°C for 90 min using a TLA-55 rotor (Beckman). The resultant pellets ...
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bioRxiv - Microbiology 2022Quote: ... The post-nuclear supernatant was adjusted to 0.8 M sucrose and loaded in the middle of a step gradient ranging from 0.1 to 1.8 M sucrose in STKM buffer and centrifuged at 50’000 g for 1 h at 4°C in a TLA-120.1 fixed-angle rotor (Beckman Coulter). LDs formed a white cushion on top of the tube and were collected by aspiration of the upper fraction of the gradient (125 µl).
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bioRxiv - Microbiology 2022Quote: ... The gradient was spun at 45,000 rpm (~200,000 x g) for 6 hours at 4 °C in a SW60Ti rotor (Beckman Coulter). Successive 0.4 mL fractions were collected by pipetting and their densities measured ...
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Ribonuclease Inhibitor and Angiogenin collaboratively regulate cell-type-specific global translationbioRxiv - Biochemistry 2024Quote: ... and were centrifuged at 39,000 rpm for 2.45 hours at 4°C using Optima XPN-80 Ultracentrifuge (Beckman Coulter). Following centrifugation ...
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bioRxiv - Molecular Biology 2024Quote: ... Virus was then purified by centrifugation through a 20% sucrose cushion at 25,000 rpm for 2.5 hours at 4 °C in a Sw32Ti rotor (Beckman Coulter). Virus pellets were resuspended in PBS and virus genomic RNA copy number was quantified by RTqPCR as described above ...
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bioRxiv - Neuroscience 2024Quote: ... by centrifugation at 5,000 x g at 4°C for 30 minutes in a fixed angle rotor (Beckman Coulter). The qEV column washed with 1x PBS was then loaded with 500 µL of concentrated supernatant followed by 2.5 mL PBS to collect 3 mL void volume ...