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Citations for Beckman :
501 - 550 of 656 citations for 10 Undecenamide N N bis 2 hydroxyethyl since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... the mixture was spun at 13,894 rpm (16,000 rcf) at 4 ºC for 10 min using the SX241.5 rotor in an Allegron X-30R centrifuge (Beckman Coulter). The beads that accumulated at the bottom of the tube were resuspended in 200 µL of EM buffer A ...
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bioRxiv - Plant Biology 2024Quote: ... using a standard protocol but with all reaction volumes reduced by 1/10 to accommodate the automation of the prep on the Echo LabCyte liquid handler (Beckman). Libraries were purified using Agencourt Ampure XP beads (Beckman Coulter) ...
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bioRxiv - Cancer Biology 2023Quote: ... The ribosomes with equal A260 in each group were loaded onto a sucrose gradient (10–50%) buffer prepared using a gradient station and centrifuged in an SW41Ti rotor (Beckman) at 4°C for 4 hours at 28,000 rpm ...
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bioRxiv - Cell Biology 2023Quote: ... The density adjusted FBS was subjected to ultracentrifugation for 25 h at 310,500 x g at 10°C in a 70Ti rotor (Beckman Coulter) before dialysis ...
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bioRxiv - Cancer Biology 2023Quote: ... 7,500 GFP+ and 7,500 RFP+ single live cells were sorted into each well of a 24 well culture plate containing low pH DMEM with 10% FBS using a MoFlo Astrios cell sorter with Summit v6.3 software (Beckman Coulter). Sorting was performed at 25psi using a 100um nozzle ...
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bioRxiv - Immunology 2023Quote: ... at 950 rpm for 10 min at 20°C and centrifuged at 15,000 rpm (Beckman Coulter Microfuge® 22R refrigerated microcentrifuge, Beckman Coulter Australia Pty Ltd ...
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Comparative membrane proteomics reveals diverse cell regulators concentrated at the nuclear envelopebioRxiv - Cell Biology 2023Quote: ... The whole cell homogenate was layered on top of 2 ml shelf of 0.8 M sucrose in HB and centrifuged at 2000 rpm for 10 min at 4°C in a JS5.2 rotor (Beckman Coulter) to yield a low speed nuclear pellet and a postnuclear supernatant ...
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bioRxiv - Cancer Biology 2023Quote: Cell death was evaluated 48 hrs after ionizing radiation (10 Gy) by using a CytoFLEX S (Beckman Coulter, Milan, Italy) instrument ...
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bioRxiv - Genomics 2023Quote: ... the supernatant was cleared by two rounds of centrifugation at 20,000 ×g (10 min, 4 °C, JA-20 rotor, Avanti J-26 XP, Beckman Coulter) followed by centrifugation at 31,900 ×g (10 min ...
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bioRxiv - Genomics 2023Quote: ... Precipitated ribosomes were pelleted at 20,000 ×g (10 min, 4 °C, JA-20 rotor, Avanti J-26 XP, Beckman Coulter). To remove residual supernatant the pellet was re-centrifuged for 5 min at 20,000 ×g ...
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bioRxiv - Genomics 2023Quote: ... The suspension was incubated on ice for 5 min and centrifuged at 20,000 ×g (10 min, 4 °C, JA-20 rotor, Avanti J-26 XP, Beckman Coulter). The pellet was re-centrifuged for 5 min and both supernatants were combined into a new tube ...
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bioRxiv - Molecular Biology 2023Quote: ... using the Illumina 16S Metagenomic Sequencing Library Preparation Protocol with the following changes: 10 μL of Amplicon product was purified using a 1:0.8 ratio of XP beads (Beckman Coulter), with beads resuspended in a final volume of 10 μL of nuclease-free water ...
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bioRxiv - Immunology 2021Quote: ... The pelleted exosomes from above supernatants were further centrifuged at 100,000g (26,450rpm) for 2 h at 4 °C (Beckman Coulter, Optima XE-90) and resuspended in PBS ...
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bioRxiv - Microbiology 2021Quote: ... Samples were centrifuged at 130,000 × g for 2 h at 4°C in a TLA100.3 rotor (Beckman Optima TLX table top centrifuge). Following centrifugation ...
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bioRxiv - Neuroscience 2021Quote: ... The post nuclear supernatant was transferred to an ultracentrifuge tube and centrifuged for 2 h at 245,000 g at 4 °C in a Beckman L90 Ultracentrifuge (Beckman Coulter, Mississauga, Canada) to pellet remaining membrane.
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bioRxiv - Neuroscience 2020Quote: ... Final 15 mL of solution was mixed by inverting the tube 10x and carefully pipetted into 2 ultracentrifuge tubes (Beckman Coulter #344059) onto the isolation buffer cushion (5 mL ...
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bioRxiv - Biochemistry 2020Quote: ... to a final volume of 1 ml and then further centrifuged (120000 g, 4°C, 2 h) in a TLA-120.2 rotor (Beckman Coulter, Brea, CA). Pellets were washed twice with 4°C cold PBS and centrifuged (120000 g ...
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bioRxiv - Microbiology 2020Quote: ... were pelleted through a 20% (wt/vol) sucrose cushion at 25,000 rpm for 2 h in an SW41 rotor (Beckman Coulter, Tokyo, Japan). Then ...
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Micro RNAs are minor constituents of extracellular vesicles and are hardly delivered to target cellsbioRxiv - Cell Biology 2020Quote: ... Cat no UZ-PA-38.5-1) at 100,000 g at 4 °C for 2 h in a swinging-bucket rotor (Beckman Coulter; SW28 or SW32).
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bioRxiv - Neuroscience 2022Quote: ... which was then sealed and placed into a hematocrit spinner (2 minutes at 13, 700 g; StatSpin CritSpin Microhematocrit Centrifuge, Beckman Coulter, Inc). Measurements were taken to calculate percent (% ...
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bioRxiv - Cancer Biology 2022Quote: ... The cell pellet was disaggregated and incubated with 2 μL of anti-pan cytokeratin-FITC (Beckman Coulter, Cat. No. IM2356U, Clone J1B3). A separate tube containing cells unstained for surface E-Cadherin was incubated after permeabilization with E-Cadherin antibody- (R&D Cat ...
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SLC15A4 favors inflammasome function via mTORC1 signaling and autophagy restraint in dendritic cellsbioRxiv - Immunology 2022Quote: ... Cells were finally washed in PBS with 0.5% BSA and 2 mM EDTA and analyzed by flow cytometry using a CytoFLEX (Beckman Coulter, Indianapolis, IN) and FlowJo software (BD Biosciences) ...
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bioRxiv - Immunology 2022Quote: ... Ultracentrifugation in a Beckman L7-65 ultracentrifuge at 25,000 rpm for 2 hours at 4°C using a Beckman SW28 rotor (Beckman Coulter, CA, USA) was performed to pellet the viruses through the sucrose cushion while soluble egg proteins were removed ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Clarified supernatants were layered over 2 ml of a 25% (w/v) sucrose solution in 13.2 ml Thinwall Polypropylene SW41 Ti ultracentrifuge tubes (Beckman-Coulter, Brea, USA) and made up to a total volume of 10 ml with PBS without calcium chloride or magnesium chloride (PBS[-]) ...
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bioRxiv - Microbiology 2019Quote: ... and viruses were pelleted via ultracentrifugation for 2 hours at 125,000 x g at 4°C using an SW55-Ti rotor (Beckman Coulter, Indianapolis, IN). Following centrifugation ...
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bioRxiv - Molecular Biology 2020Quote: ... was subjected to centrifugation at 100,000g for 2 h in an Optima MAX-XP benchtop ultracentrifuge with a TLA-100.3 rotor (Beckman Coulter, Inc., Brea, CA). Pellets were resuspended in 100 µl PBS and stored at −80 °C.
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bioRxiv - Pathology 2020Quote: ... Hemocytes stained with 4’-6-diamidino-2-phenylindole (DAPI) were analyzed on a CytoFLEX flow cytometer with CytExpert software (Beckman-Coulter, USA). Plots of side scatter SSC-A versus forward scatter FSC-A were used for visualization of cell groups ...
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bioRxiv - Biochemistry 2019Quote: ... in iso-osmolar RB150/Mg2+ (containing a reduced level (2%) of glycerol) and 80µl were transferred to 7 × 20 mm polycarbonate tubes (Beckman Coulter, Brea CA), overlaid with 80µl of 35% ...
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bioRxiv - Neuroscience 2020Quote: ... Homogenates were layered on top of 1 ml of 0.85 M sucrose in 2 mM EGTA and centrifuged at 75,000 g for 30 min in a Beckman FW-27 rotor (Beckman, Fullerton, CA, USA). This step was repeat twice ...
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bioRxiv - Microbiology 2021Quote: ... soil was thoroughly mixed with 20 ml of 0.01 M CaCl2 for 30 min on a Elmi Intelli-Mixer RM-2 (Elmi Tech Ltd, Latvia) and subjected to centrifugation in an Allegra X-22R centrifuge (Beckman Coulter, USA) at 3900 rcf for 20 min and to remove inorganic S (Dedourge et al. ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were centrifuged at 60,000 rpm at 4°C for 2 h on an SW65 Ti ultracentrifuge rotor (Beckman Coulter, California, USA). The pellets were gently washed by dispensing and removing 500 µl of ice-cold RNase-free water ...
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bioRxiv - Cell Biology 2023Quote: ... cells were blocked in 2% human serum and PBS for 30 min at 4°C and stained with DC-SIGN (Beckman Coulter #A07407), CD80 (BioLegend #305214) ...
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bioRxiv - Plant Biology 2023Quote: ... 700 μl of obtained extract was applied to a chilled sucrose density gradient and centrifuged at 4 °C for 2 hours 45 minutes at 151,000 ×g using the SW 41 Ti rotor (Beckman Culture Life Sciences). After centrifugation ...
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bioRxiv - Molecular Biology 2023Quote: ... The lysates were loaded onto the sucrose gradients and centrifuged at 40’000 rpm for 2 h at 4°C using the SW 41 Ti rotor (Beckman Coulter, Cat# 331362). Subsequently ...
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bioRxiv - Biochemistry 2023Quote: ... Samples were centrifuged at 100,000 × g for 2 hr at 4 °C (Type 50.2 Ti Fixed angle ultracentrifuge rotor, Beckman Coulter, Brea, CA, USA). The supernatants were aspirated ...
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bioRxiv - Microbiology 2023Quote: ... the prepared DNA library was size-selected on a 2% agarose gel (410–470 bp) using the Agencourt SPRI system to reduce mitochondrial contamination (Beckman Coulter, Inc.). After library prep ...
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bioRxiv - Plant Biology 2023Quote: ... DNA extracts were purified using a bead clean up method with a 2:1 ratio of Ampure XP beads (Beckman Coulter, USA) to DNA elution ...
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bioRxiv - Immunology 2023Quote: ... Bacteria cultures were collected and discarded by centrifugation for 60 min at 4,000-8,000 x g and the supernatants were subjected to high-speed centrifugation at 11,9000 x g for 2-3 h at 4°C (Beckman Coulter Optima Ultracentrifuge). The pellets containing the OMVs were washed with PBS ...
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bioRxiv - Biophysics 2023Quote: ... The virions were concentrated by ultracentrifugation through a 20% (w/v) sucrose cushion (2 h at 28,000 rpm in a SW32 rotor [Beckman Coulter Life Sciences]). The pellet was resuspended in PBS ...
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bioRxiv - Genetics 2023Quote: ... in-house VLPs were produced using the same protocol followed by ultracentrifugation at 24,000 RPM for 2 hours (SW 28 Beckman swinging bucket rotor) to concentrate the VLPs and avoid diluting the HSPCs culture media ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were pelleted at 280’000 x g for 10 min at 25 °C in an Optima XP centrifuge with a TLA-100 rotor (Beckman-Coulter). Supernatant and pellet fractions were separated on a 10 % SDS-PAGE gel and proteins were visualized by Coomassie Blue staining ...
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bioRxiv - Molecular Biology 2021Quote: ... About 1,5 mg of protein was loaded on a 10–50% sucrose gradient and centrifuged for 2h45min at 39 000 rpm in an Optima L-100XP Ultracentrifuge (Beckman-Coulter) using a SW41 rotor (Beckman) ...
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bioRxiv - Cell Biology 2022Quote: ... Yeast cells were stained with 5ug\ml propidium iodide for 10 minutes and samples were examined in CytoFlex flow cytometer (Beckman Coulter) using PE channel ...
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bioRxiv - Molecular Biology 2021Quote: ... pombe cells were pelleted down for 10 min at 5000 rpm at 4°C (Rotor: F10-6X500, FiberLite Beckman J2-MC). The pellet was washed with buffer 1 (HEPES- 20 mM ...
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bioRxiv - Molecular Biology 2020Quote: ... and centrifuged through a 10–35% sucrose gradient for 19 h at 16,000 rpm in a SW28 rotor (Beckman Coulter, Inc.). Fractions containing the 70S peaks were pooled and kept at −80℃ for further use.
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bioRxiv - Bioengineering 2022Quote: ... The 60-70 mL were then divided equally into two 50-mL centrifuge tubes and spun down outside the anaerobic chamber at 4 °C and 3500 rpm for 10 min using an Allegra 64R ultracentrifuge (Beckman Coulter). Next ...
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bioRxiv - Bioengineering 2022Quote: ... Each 250 mL culture was divided equally into six 50-mL centrifuge tubes and spun down at 4 °C and 3500 rpm for 10 min using an Allegra 64R centrifuge (Beckman Coulter). The supernatant was discarded and 6 mL of ice-cold 10% glycerol was used to wash and combine the six cell pellets into one suspension ...
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bioRxiv - Genetics 2020Quote: ... The homogenates were incubated on ice for 15–20 min and then centrifuged at 3000xg for 10 min at 4°C in a GPR Data Settop centrifuge (Beckman, Inc.). The supernatant solutions were centrifuged at 15,800xg for 15 min at 4°C in a microcentrifuge (Model 5042 ...
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bioRxiv - Cell Biology 2021Quote: ... The sample was overlaid with decreasing concentrations of Nycodenz (30%, 20%, 10% and 0%) and centrifuged in a SW55 rotor (Beckman Coulter) 2h 30 min at 55000 rpm and 4°C ...
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bioRxiv - Plant Biology 2021Quote: ... Counting was performed twice for each sample (10 min per sample) with a ß-scintillation counter Beckman LS6000TA (Beckman Coulter, USA). Roots of two plants identically grown and processed but not exposed to the incubation solution were used to determine background counts ...