Labshake search
Citations for Beckman :
501 - 550 of 1775 citations for 1 4 Bromo 3 methylphenyl ethanone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2023Quote: ... the C-tubes were quickly spun in a chilled (4°C) Allegra-30R centrifuge (#B08708, Beckman Coulter) with an SX4400 swinging bucket rotor to collect the samples in the bottom of the tubes ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were harvested by centrifugation at ∼6250g for 15 min at 4°C (Beckman Coulter centrifuge, JLA8.1000).
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bioRxiv - Biochemistry 2023Quote: ... The lysate was then centrifuged at ∼28500g for 1h at 4°C (Beckman Coulter centrifuge, JA-14) and the supernatant incubated for 1h ...
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bioRxiv - Cell Biology 2022Quote: ... and 4 mM DTT)) were added to the flow chambers after centrifugation in an ultracentrifuge (Beckman Airfuge) at 119,000xg for 5 minutes ...
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bioRxiv - Microbiology 2022Quote: ... 4 °C) in an Optima LE-80K ultracentrifuge with a 50.2 Ti rotor (Beckman-Coulter Life Sciences). Supernatants were removed ...
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bioRxiv - Neuroscience 2022Quote: ... Insoluble material was removed by ultracentrifugation (1h, 4°C, 40,000 rpm, Ti70 rotor, Beckman Coulter Cat#337922). The fragments were immobilized onto glutathione Sepharose (Millipore/Cytiva Cat#17-5132-02 ...
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bioRxiv - Biophysics 2023Quote: ... the mixture was centrifugated for 30 min at 12,000 rpm and 4 °C (Beckman J2-21, USA), and iVCL supernatant was collected ...
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bioRxiv - Biophysics 2023Quote: ... and centrifuged at 5000 xg for 30 minutes at 4°C and subsequently at 10000 xg (Beckman Coulter Optima L-90K with a SW40Ti rotor) ...
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bioRxiv - Biochemistry 2023Quote: ... After ultracentrifugation for 1h at 258,488 x g at 4°C using a TLS-55 rotor (Beckman), the fractions were analysed by SDS-PAGE and the positive fractions were concentrated ...
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bioRxiv - Biophysics 2024Quote: ... Aggregates were removed by ultracentrifugation at 4 °C at 100,111 x g in a TLA100 rotor (Beckman). Fresh Halo-fluorophore conjugated proteins were prepared for each experiment.
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bioRxiv - Bioengineering 2024Quote: Microbubble concentration and number- and volume-weighted size distributions were measured with a Multisizer 4 (Beckman Coulter). Microbubble concentration (ci ...
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bioRxiv - Cancer Biology 2024Quote: ... Lysates were then separated by centrifugation at 30,000 rpm and 4°C in an SW41Ti rotor (Beckman) for 18 hours ...
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bioRxiv - Plant Biology 2024Quote: ... The cell concentration was measured in triplicate with a particle counter (Multisizer 4 Counter, Beckman Coulter, USA) after each disruption cycle ...
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bioRxiv - Microbiology 2024Quote: ... at 28 K rpm for 2 h at 4°C in an SW32 swing-bucket rotor (Beckman). SG3ΔEnv virions were thoroughly resuspended using 500 μl Hepes/Salt buffer per tube ...
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bioRxiv - Neuroscience 2024Quote: ... An equimolar pool of 4 barcoded libraries was cleaned up with 0.8x AMPure XP beads (Beckman Coulter). The pools were quantified and assessed with Qubit and Femto ...
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bioRxiv - Genomics 2024Quote: ... the virus was ultra-centrifuged at 24,000 rpm at 4 C° for 2 h using SW32Ti (Beckman) and the virus pellets were resuspended in RPMI media with 10% FBS to result in a 20-fold concentrated virus and stored at −80 C°.
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bioRxiv - Biochemistry 2024Quote: ... The gradients were run overnight at 4 ℃ in an SW 41 Ti swinging bucket rotor (Beckman Coulter) and subject to 5.45×1011 ω2t total centrifugal force (27,000 rpm ...
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bioRxiv - Biochemistry 2024Quote: ... The crude membrane fraction was isolated by ultracentrifugation (100000g, 45 min, 4 °C, Beckman Coulter rotor Ti70). Membranes were resuspended in Buffer A at 5 mg/mL and stored at -80°C for later use.
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bioRxiv - Biophysics 2024Quote: ... the gradient was centrifuged at 107,000xg for 16 hours at 4 °C (SW 40 rotor, Beckman Coulter). After centrifugation ...
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bioRxiv - Cell Biology 2020Quote: ... A 1:1 Ampure XP (Beckman Coulter) PCR purification step was performed followed by assessment of size distribution using an Agilent high-sensitivity DNA chip (Agilent technologies ...
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bioRxiv - Microbiology 2020Quote: ... and overlaid with 6 ml of 30% and 3 ml of 5% sucrose (w/v) followed by centrifugation at 32,000 rpm in SW41 rotor (Beckman Coulter) for 20 h ...
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bioRxiv - Molecular Biology 2020Quote: ... Debris was clarified by centrifuging at 18,000 ×g followed by fractionation on a discontinuous sucrose gradient (10-50 %) by ultracentrifugation at 39,000 rpm for 3 h in an SW41-Ti rotor (Beckman coulter). Monosome fractions were collected by pumping of Fluorinert FC-40 and using a fraction collector by measuring UV trace ...
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bioRxiv - Molecular Biology 2020Quote: ... The gradient was formed by adding 3 mL of the iodixanol solutions to a 17 mL polypropylene tube (Beckman Coulter). Ultracentrifugation was performed using a SW Type 32.1 Ti rotor in Optima XPN-100 Ultracentrifuge (Beckman Coulter ...
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bioRxiv - Cancer Biology 2020Quote: The barcode split of the cDNA amplification reaction (from 10x Single Cell 3’ v3 Step 2.2) was purified via 1.2x SPRI Select (Beckman Coulter #B23317). cDNA products were eluted in 40 µL of EB ...
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bioRxiv - Neuroscience 2020Quote: ... reverse 5’-CTTA TTAGTCAGTCAGAAACAAC-3’).ThePCR amplicon was purified using RNAse-free SPRI beads (Agencourt RNAclean XP, Beckman-Coulter A63987) and then used for in vitro transcription with the HiScribe T7 ARCA mRNA Kit (with tailing ...
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bioRxiv - Microbiology 2021Quote: 3 mL of pseudoparticles were pelleted using a TLA-55 rotor with an Optima-MAX-E ultracentrifuge (Beckman Coulter, Inc.) for 2 hours at 42,000 rpm at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... Supernatants (3 ml) were loaded on the top of step gradients preformed in polyallomer tubes (14 by 89 mm, Beckman), containing 2 ml each of 1.82 ...
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bioRxiv - Molecular Biology 2020Quote: ... 1.3 M, 2.5 M) as indicated in the method (Vella et al., 2017) in ultra-clear SW40Ti tubes (Beckman Coulter). The sucrose gradients were centrifuged at 200,000 x g avg at 4°C for 173 min using a SW40Ti rotor (Beckman Coulter) ...
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bioRxiv - Microbiology 2020Quote: ... was overlaid on 25% sucrose in PBS and centrifuged at 26,000 rpm for 3 h at 4C using SW28 rotor (Beckman Coulter). Pellets were dissolved in PBS overnight at 4C and 1/3 of used for RNA was extraction using Macherey Nagel Virus RNA isolation kit without carrier RNA according to the manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2020Quote: ... Samples are visualized on Cytoflex S, 3 lasers (488 nm, 405 nm and 638 nm) and 13 detectors (Beckman Coulter). Live cells were gated based on side scatter and forward scatter ...
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bioRxiv - Molecular Biology 2020Quote: ... and the cleared supernatant was loaded on a 3 to 30% iodixanol gradient (OptiPrep) and ultracentrifuged for 5h at 36,000 rpm in an SW60 rotor (Beckman Coulter). Fractions were collected manually through aspiration and RNA or proteins were isolated for further analysis.
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bioRxiv - Microbiology 2020Quote: ... The filtered supernatant was then spun through a 20% sucrose cushion at 48,000 rpm for 3 hours in a SW55Ti rotor (Beckman Coulter). The SVP pellets were resuspended in a 4X non-reducing buffer (Alfa Aesar ...
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bioRxiv - Microbiology 2021Quote: ... 60% w/v) and centrifuged at 64,000 x g for 3 hours in A27-8×50 mL rotor (Beckman Coulter). The visible virus bands were collected ...
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bioRxiv - Molecular Biology 2023Quote: ... The reactions were then layered on 100 μL of 32% sucrose cushion and centrifuged at 42,800 rpm for 3 hours in a Beckman TLA 100 rotor using the Optima-Max TL ultracentrifuge (Beckman Coulter). After centrifugation ...
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bioRxiv - Microbiology 2022Quote: ... HUVECs or LECs were infected for 3 days and the GFP positive population was quantified by CytoFLEX S (Beckman Coulter). Here ...
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bioRxiv - Bioengineering 2024Quote: ... The sample tubes were then centrifuged at 347,000 × g and 20°C for 3 hours using a Type 70 Ti rotor (Beckman Coulter). 1-mL fractions were collected from the top of the tube ...
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bioRxiv - Microbiology 2024Quote: ... The flow cytometry analysis of the bacterial cells present in the suspension was performed using a Cytoflex flow cytometer (CytoFLEX 3; Beckman) as previously described (Supplementary Fig ...
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bioRxiv - Cancer Biology 2024Quote: ... The fragments were repaired the 3’and 5’overhangs using End repair mix (component of SureselectXT) and purified using Agencourt AMPure XP Beads (Beckman). The purified fragments were added with ‘A’ tail using A tailing Mix (component of SureSelectXT ...
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bioRxiv - Microbiology 2022Quote: ... The sample-containing tube was spun at 347,000 × g and 20°C for 3 hours using Type 70 Ti rotor (Beckman Coulter). 1-mL fractions were collected from the top of the tube ...
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bioRxiv - Biochemistry 2020Quote: ... Lysates were cleared by ultracentrifugation (25000 rpm for 30 min at 4°C using a Beckman Ti45 rotor). Supernatant was filtered (0.45 μm ...
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bioRxiv - Biochemistry 2020Quote: ... Lysates were cleared by ultracentrifugation (40000 rpm for 30 min at 4°C in a Beckman Ti45 rotor). Supernatant was filtered (0.45 μm ...
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bioRxiv - Biochemistry 2020Quote: ... Lysates were cleared by ultracentrifugation (40000 rpm for 30 min at 4°C in a Beckman Ti45 rotor). Supernatant was filtered (0.45 μm ...
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bioRxiv - Cell Biology 2020Quote: ... and then centrifuged (300 g for 5 min at 4 °C) (Allegra X-30R Centrifuge, Beckman Coulter, USA) to obtain the myofiber-associated cell pellet ...
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bioRxiv - Cell Biology 2020Quote: ... and then centrifuged (300 g for 5 min. at 4 °C) (Allegra X-30R Centrifuge, Beckman Coulter, USA) to obtain the myofiber-associated cell pellet ...
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bioRxiv - Molecular Biology 2021Quote: ... RNA concentration of the cleared lysate was measured by nanodrop and 500 μg of this RNA was loaded onto the gradient and run for 2.5 hr at 220,000 g and 4°C in an SW41-rotor (Beckman). Gradients were then run at 850 μL/min in a density gradient fractionation system (Teledyne Isco) ...
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bioRxiv - Molecular Biology 2021Quote: ... Gradients were centrifuged at 36000 RPM for 2 hours at 4°C in a SW41 rotor (Beckman Coulter) and then fractions were collected using a gradient collector (Teledyne ISCO gradient station ...
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bioRxiv - Microbiology 2022Quote: ... for 3h at 4°C in a Beckman ultracentrifuge with the SW40ti rotor head (Beckman, Brea, California, USA) using open top polyallomer tubes (Seton Scientific ...
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bioRxiv - Bioengineering 2021Quote: ... The resulting solution was centrifuged at 164,000 × g (30,000 rpm) for 4 h at 20°C (Optima XPN-80 Ultracentrifuge, Beckman) to remove any remaining nanotube aggregates.
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bioRxiv - Bioengineering 2020Quote: ... larger vesicles were depleted by centrifugation for 30 minutes at 10,000xg at 4°C (SW28 rotor, Beckman Coulter). In the final step ...
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bioRxiv - Biochemistry 2020Quote: ... 300mM NaCl (pH 7.0) was performed at 4 ⁰C on an Optima XL-A Analytical Ultracentrifuge (Beckman Coulter). Equilibrium radial distributions at 10,000 and 15,000 RPM of Sfh5 at concentrations of 10 ...