Labshake search
Citations for Beckman :
501 - 550 of 1177 citations for 1 2 Chloropyridin 3 yl 3 3 dimethylazetidin 2 one since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biophysics 2023Quote: ... The virions were concentrated by ultracentrifugation through a 20% (w/v) sucrose cushion (2 h at 28,000 rpm in a SW32 rotor [Beckman Coulter Life Sciences]). The pellet was resuspended in PBS ...
-
bioRxiv - Genetics 2023Quote: ... in-house VLPs were produced using the same protocol followed by ultracentrifugation at 24,000 RPM for 2 hours (SW 28 Beckman swinging bucket rotor) to concentrate the VLPs and avoid diluting the HSPCs culture media ...
-
bioRxiv - Microbiology 2021Quote: ... and extracellular vesicles including exosomes and virions were pelleted by ultracentrifugation at 141,000 g at 4 °C for 2 h in a swinging-bucket rotor (SW28; Beckman Coulter, Brea, CA, USA). The pellet was resuspended in filtered PBS and centrifuged at 18,300 g and 4°C for 10 min to remove insoluble aggregates ...
-
bioRxiv - Microbiology 2020Quote: ... The clarified supernatant was overlaid onto a 20% (w/v) sucrose cushion in Tris-NaCl-EDTA buffer and centrifuged at 134,000 g for 2 hours (Beckman Coulter 30 Ti rotor). The resulting viral pellet was suspended in VCM by repeat pipetting and aliquots stored in cryovials at minus 70°C until needed ...
-
bioRxiv - Genomics 2020Quote: ... were layered onto a 1.8M sucrose cushion and ultra-centrifuged at 24,400 rpm at 4°C for 2 hours using a SW28 swinging bucket rotor (Beckman Coulter Optimal centrifuge #L90K). The nuclear pellet was re-suspended in 2% BSA in 1X PBS ...
-
bioRxiv - Microbiology 2020Quote: ... EMVs were obtained by ultracentrifugation from 4 mL of filtrate at 100,000 × g (average centrifugal force) and 4°C for 2 h with an OptimaX centrifuge (Beckman Coulter, Brea, CA, USA). The pellets were resuspended in 400 μL of Dulbecco’s phosphate-buffered saline (DPBS ...
-
bioRxiv - Neuroscience 2022Quote: ... The tubes were sealed and centrifuged at 50,000 rpm at 4°C for 2 h in an OptimaTM L-90K ultracentrifuge using the 70.1Ti rotor (Beckman Coulter, Brea, CA, USA). After the centrifugation ...
-
bioRxiv - Cell Biology 2020Quote: ... 3 μM or 10 μM from a 2 mM or 10 mM stock solution using a Biomek FX with 384-well head (Beckman Coulter, Indianapolis, IN). The DMSO concentration was kept below or at 0.5% v/v ...
-
bioRxiv - Physiology 2022Quote: ... the library preparation step followed the CEL-Seq 2 protocol85: the RT product was digested by ExoI and HinFI and purified using AMpure XP beads (Beckman Coulter™, USA). Then ...
-
bioRxiv - Microbiology 2024Quote: ... then the colonies were diluted and inoculated into the Neg MIC EN-2J panel containing β-lactams listed in Table 2 and incubated in MicroScan Walkway40 Plus (Beckman Coulter Inc.). Breakpoint criteria for susceptible (S) ...
-
bioRxiv - Molecular Biology 2024Quote: ... Each compound was dissolved in DMSO at a concentration of 2 mM and distributed into an Echo Qualified 384-Well Polypropylene Microplates (Beckman Coulter, 001-14615). Using an Echo 655 Liquid Handler ...
-
bioRxiv - Microbiology 2022Quote: ... The success of the amplifications was checked in a 2% agarose gel and PCR products were subsequently purified using Agencourt AMPure XP magnetic beads kit (Beckman Coulter, Brea, CA, USA), quantified with the QuantiFluor™ dsDNA System (Promega ...
-
bioRxiv - Microbiology 2020Quote: ... and layered on top of a two-step sucrose gradient (20%/50% w/v in HNE) and centrifuged for 2 h at 154 000 x g at 4 °C in a SW41 rotor (Beckman Coulter, Woerden, the Netherlands) to clear from cell debris ...
-
bioRxiv - Biochemistry 2023Quote: ... cells were treated with the drugs (20 µM to a suspension at 500,000 cells/mL in PBS containing 2 % FBS at 37°C) and directly analyzed by flow cytometry (Gallios, Beckman-Coulter, 405/488 nm laser) over 2 h ...
-
bioRxiv - Microbiology 2024Quote: ... EMVs were obtained by ultracentrifugation from 3 mL of filtrate at 100,000 × g (average centrifugal force) and 4 °C for 2 h with 50Ti rotor and Optimax centrifuge (Beckman Coulter, Brea, CA, United States). The supernatant after removing EMVs was collected as PVF ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... the two amplifications obtained (one per set) were purified with AMPure XP beads (Beckman-Coulter) at a 1:1 ratio according to the manufacturer’s recommendations and pooled at equimolar concentrations ...
-
bioRxiv - Microbiology 2023Quote: ... CsCl step gradients were prepared in one clear ultracentrifuge tube (Beckman-Coulter, catalog number 344059) by slowly pipetting the solutions in the following order ...
-
bioRxiv - Cancer Biology 2020Quote: ... Filtered media containing lentiviral particles were centrifuged at 24000rpm at 20°C for 2 hrs in 90Ti rotor in ultra-centrifuge (Beckman Coulter Optima TM L-100K). Viral particle pellet was obtained after discarding supernatant post centrifugation and suspended in 100 μl OptiMEM media for overnight at 4°C.The collected lentivirus suspension was used for transfection or stored for later use at −80°C ...
-
bioRxiv - Physiology 2020Quote: ... one bird per pen in Experiment 2) and centrifuged for 5 min at 17013 × g using a Beckman microfuge (Model E 348720, Beckman Instruments, INC, Palo Alto, CA). Then the viscosity of ileal supernatant was measured using a Brookfield cone-plate digital viscometer (Model LVDV-Ⅲ ...
-
bioRxiv - Microbiology 2024Quote: ... Viruses were collected 48 hours post-transfection and pelleted in DPBS over a 10% sucrose cushion at 25,000 RPM for 2 hours using a SW32Ti rotor (Beckman Coulter Life Sciences, Brea, CA, USA). Virus pellet was then resuspended in labeling buffer (50 mM HEPES pH 7.0 ...
-
bioRxiv - Microbiology 2024Quote: ... Virus was collected 48 hours post-transfection and pelleted over a 10% sucrose cushion at 25,000 RPM for 2 hours at 4 °C using a SW32Ti rotor (Beckman Coulter Life Sciences, Brea, CA, USA). Pellets were resuspended in DMEM (Gibco ThermoFisher Scientific ...
-
bioRxiv - Cancer Biology 2021Quote: ... and RNA was purified with one volume of Agencourt RNAclean XP beads (Beckman Coulter, Cat #A63987) according to manufacturer's instructions ...
-
bioRxiv - Molecular Biology 2023Quote: ... and DNA fragments were subsequently recovered with one volume of AMPure XP beads (Beckman Coulter, A63880) mixed with one volume of 30% of PEG8000 in 1.25M NaCl ...
-
bioRxiv - Microbiology 2023Quote: ... another 2 mL of staining bacterial samples was analyzed with a flow cytometry to check cell viability (Beckman CytoFLEX FCM, USA, Channel FL2-A: PE-A).
-
bioRxiv - Microbiology 2020Quote: ... Double stranded viral cDNA was purified using one volume of Ampure XP per manufacturer’s instructions (Beckman Coulter). Once beads were completely dry ...
-
bioRxiv - Immunology 2021Quote: ... lymphocytes were stained for one hour at room temperature in the dark with CD56-P5.5 (Beckman Coulter) and CXCR4-PE (BD Biosciences) ...
-
bioRxiv - Developmental Biology 2021Quote: ... The four reactions were combined into one tube and size selected using Ampure XP beads (Beckman Coulter). Final Hi-C libraries were quantified using Qubit dsDNA HS assay kit and a DNA HS kit on a 2100 Bioanalyzer (Agilent) ...
-
bioRxiv - Neuroscience 2024Quote: ... The two reactions were pooled in one Eppendorf tube and 70 µl of AMPureXP beads (Beckman Coulter) were added to the sample and incubated for 5 minutes at room temperature ...
-
bioRxiv - Plant Biology 2021Quote: ... The supernatant was transferred into new centrifuge tubes and centrifuged at 40,000g (P40) or 100,000g (P100) for one hour (4°C, TLA100.3, Optima TLX Ultracentrifuge; Beckman Coulter) to pellet EVs and other particles as noted in figure legends ...
-
bioRxiv - Biochemistry 2022Quote: ... and one × 104 gated events per sample were recorded on a CytoFLEX S (Beckman Coulter, Brea, CA, USA). Subsequent analysis was performed with Kaluza Flow Cytometry software (version 1.3 ...
-
bioRxiv - Genomics 2020Quote: ... The library was purified one time with 1.2 volumes of AMPure PB beads (Beckman Coulter, Brea, CA, USA). The size and amount of library was checked again using the Bioanalyzer 2100 system and the Qubit fluorometer with Qubit High Sensitivity (HS ...
-
bioRxiv - Bioengineering 2023Quote: ... EVs were pelleted from conditioned media by spinning at ∼100,000 x g (29,500 rpm) for one hour in a SW32 Ti rotor (Beckman), and the supernatant was discarded ...
-
bioRxiv - Genomics 2024Quote: ... the products of the two amplifications (one per primer set) were purified with AMPure XP beads (Beckman Coulter) at a 1:1 ratio according to the manufacturer’s recommendations and pooled at equimolar concentrations ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... the products of the two amplifications (one per primer set) were purified with AMPure XP beads (Beckman Coulter) at a 1:1 ratio according to the manufacturer’s recommendations and pooled at equimolar concentrations ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... The filtered supernatant from one 400 ml batch was then pelleted (1.5 hour at 24 000 rpm at 4 °C with Beckman Coulter L-90K centrifuge and 45 Ti rotor ...
-
bioRxiv - Systems Biology 2023Quote: ... Two libraries were prepared and sequenced: one with size-based selection for mRNA >4 kb using AMPure beads (Beckman) and a second library with no size selection.
-
bioRxiv - Immunology 2024Quote: ... AML patients and one t(8;21) AML patient-derived xenograft (PDX) sample (RL048)20 by flow cytometry (Cytoflex LX, Beckman Coulter ...
-
bioRxiv - Cell Biology 2024Quote: ... and the membranes were pelleted at 100,000 g for one hour at 4°C in a TLA 100.3 rotor (Beckman-Coulter). Afterwards the supernatant was taken off and stored at −20°C (“S100” ...
-
bioRxiv - Cell Biology 2024Quote: ... 10% and 0% in buffered sucrose on top of one another in 5 ml Ultra-Clear centrifuge tubes (Beckman). Gradients were centrifuged for 16 h at 100,000 x g and 4 °C ...
-
bioRxiv - Immunology 2021Quote: ... the pellets were resuspended in cold PBS and transferred to one Ultra-Clear 13.2 mL tube (Beckman Coulter, ref 344059) and centrifuged again at 100,000g 4°C for 1 hour and 30 minutes ...
-
bioRxiv - Cancer Biology 2024Quote: ... at least 10,000 events were acquired per sample using one of two flow cytometers: either a flow cytometer (CytoFLEX and CytExpert 2.4, Beckman Coulter), which utilizes a 488-nm laser paired with a 530/30 nm bandpass filter ...
-
bioRxiv - Genomics 2021Quote: ... After one wash with MACS buffer the percentages of IAV infected cells were determined with a MoFlo Astrios Cell Sorter (Beckman Coulter) and the uninfected gating control was defined using uninfected cells stained with both the primary and the secondary antibodies.
-
bioRxiv - Genomics 2021Quote: ... One milliliter of the precleared plasma or serum was transferred to a thick-wall polypropylene tube (Beckman Coulter, Brea, CA, USA) and centrifuged in an OptimaMAX (Beckman Coulter ...
-
bioRxiv - Cell Biology 2021Quote: ... GFP-positive cells were sorted to one cell per well into 96-well plates containing fresh culture medium using a MoFlo Cell Sorter (Beckman Coulter) and clones were gradually expanded to 6-well format over the course of several weeks ...
-
bioRxiv - Plant Biology 2022Quote: ... The supernatant was then recovered and centrifuged at 30,000 gav for one hour in the Ti45 rotor and in a Beckman L-80 ultracentrifuge (Beckman-Coulter, Mexico). The resulting pellet was re-suspended in 5 mL of modified CIB medium and centrifuged at 16,000 gav for 10 min in 5415R refrigerated centrifuge (Eppendorf ...
-
bioRxiv - Physiology 2023Quote: ... The compounds were dissolved at a concentration of 10 mM in DMSO and one batch of this library was distributed in a 384 well LDV microplate (LP-0200, Beckman coulter). For automated screening assay ...
-
bioRxiv - Bioengineering 2024Quote: ... Sonicated suspensions were ultracentrifuged at 58,000 x g for one hour using an Optima Max-XP Ultracentrifuge (Beckman Coulter; Brea, CA). The top 75% of the suspension was collected ...
-
bioRxiv - Microbiology 2024Quote: ... Two µL of each final product was pooled into one tube and solid-phase reversible immobilisation (SPRI) beads (Beckman Coulter, USA) were used to remove excess primers ...
-
bioRxiv - Cell Biology 2024Quote: ... The remaining half cells were subjected to single-cell sorting and deposited into a 96-well plate at one cell per well using the CytoFLEX SRT Cell Sorter (Beckman, USA). After approximately 10 days of culture ...
-
bioRxiv - Genetics 2022Quote: ... Four μL from each successful reaction were combined into one 1.5 ml microfuge tube and the primers were removed using Agencourt AMPure™ XP beads (Beckman Coulter #A63881).