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Citations for Beckman :
451 - 500 of 563 citations for N 2 2 2 2 Aminoethyl amino ethyl amino ethyl amino ethyl 9 12 15 octadecatrienamide since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... Gradients were centrifuged at 50,000 rpm and 12°C for 100 minutes in the Optima XE-100 Ultracentrifuge (Beckman Coulter). Next ...
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bioRxiv - Microbiology 2021Quote: ... The supernatant was transferred to an ultracentrifuge tube and overlayed with layers of Lysis Buffer A with 12% Ficoll and Buffer B with 8% Ficoll (AAB2209518, Fisher) and centrifuged at 28,500 rpm for 45 min (Beckman 42.1). Afterwards ...
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bioRxiv - Neuroscience 2020Quote: ... The V and VI fractions were diluted to a total volume of 12 mL with dfPBS and centrifuged at 140,000 × g for 70 min at 4°C (# Optima-XE SW41 Beckman Coulter), and each pellet were resuspend with 30 μl of dfPBS ...
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bioRxiv - Microbiology 2023Quote: ... Proteoliposomes were then concentrated by ultracentrifugation (30 min; 100.000 g; 12 ºC) in an Optima Max XP Tabletop Ultracentrifuge (Beckman Coulter), resuspended in Internal Buffer and kept at 12 ºC.
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bioRxiv - Cell Biology 2023Quote: ... The reaction was stopped with BRB80 supplemented with 0.5 % glutaraldehyde and spun onto 12 mm coverslips in a centrifuge tube (Beckman & Coutler) containing BRB80 buffer using the SW32Ti rotor (Beckman & Coulter ...
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bioRxiv - Neuroscience 2024Quote: ... at a ratio of 1:2.3 before layering on 3 ml of SCB in a 12 ml Thinwall Ultra-clear centrifuge tubes (Beckman Coulter). Samples were centrifuged at 30,000 xg for 45 min at 4 °C using a SW41 Ti swinging bucket rotor (Beckman Coulter ...
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bioRxiv - Biophysics 2020Quote: ... lysate was centrifuged at 5 °C with 13000 rpm for 15 min by Avanti J-E Ultra Centrifuge (Beckman Coulter). Supernatant from centrifugation was filtered with 0.45 μm SFCA syringe filters (Sigma Aldrich ...
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bioRxiv - Biophysics 2022Quote: ... The cell suspension was centrifuged for 5 min at 2000 g (Centrifuge Beckman Coulter Allegra X-15-R, Rotor Sx4750A)) and 4°C ...
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bioRxiv - Plant Biology 2020Quote: ... The sucrose gradient was centrifuged at 154,300 × g for 15 h at 4 °C using a SW 41 Ti rotor (Beckman Coulter). The LHCII proteoliposomes separated from empty liposomes were collected dropwise from the bottom of the tube and used for analysis.
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bioRxiv - Plant Biology 2021Quote: ... The plant debris was removed by centrifuging at 16 000 g for 15 minutes at 4°C in a JA-25.50 rotor and Avanti J- 20 XP centrifuge (Beckman Coulter). The clear supernatant was then gently poured on top of 8 ml sucrose cushion (100 mM Tris·HCl (pH 8.0) ...
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bioRxiv - Microbiology 2021Quote: ... bacteria were harvested by centrifugation at 4000 x g for 15 min (Microfuge 20R; Beckman Coulter, La Brea, CA, USA). The pellet was washed twice in PBS ...
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bioRxiv - Cell Biology 2020Quote: ... cells and larger debris were removed by 2000 × g centrifugation of cell suspensions for 15 minutes (Rotor: JS-5.3; Beckman Coulter). MSC-free CM were stored at −20°C until usage ...
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bioRxiv - Biochemistry 2022Quote: ... 40 μl of the mixture was transferred to an ultracentrifuge tube and centrifuged at around 100,000 x g for 15 minutes at room temperature using the Airfuge (Beckman Coulter). After centrifugation ...
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bioRxiv - Microbiology 2022Quote: ... and layered onto a preformed 11 mL 5% to 15% (wt/vol) Ficoll gradient in a 13.2 mL thin-wall polyallomer ultracentrifuge tube (Beckman Coulter). Gradients were centrifuged at 12,000 rpm for 2 h at 4°C in an SW41 Ti swinging-bucket rotor in a Sorvall Discovery SE ultracentrifuge ...
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bioRxiv - Cell Biology 2020Quote: ... Cleared supernatants obtained from cytoplasmic lysates were loaded on a linear 15%-50% sucrose gradient and ultracentrifuged in a SW41Ti rotor (Beckman) for 1 h and 40 min at 180,000 x g at 4°C in a Beckman Optima LE-80K Ultracentrifuge ...
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bioRxiv - Cell Biology 2020Quote: ... This crude mitochondrial pellet was further purified on a discontinuous percoll gradient (buffer composition 15/23/40% sucrose) by centrifugation (40,000 x g, 1hr, 4°C) in an SW41 rotor (Beckman Coulter) and enriched mitochondria were collected from the 23-40% interface (adapted from [76]) ...
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bioRxiv - Biophysics 2023Quote: ... Reactions were centrifuged at 400,000 g at 20 °C for 15 min in polycarbonate centrifuge tubes (Beckman Coulter Inc. USA). The pellets were resuspended in 40 μl reaction buffer ...
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bioRxiv - Genomics 2023Quote: ... The ribosomes were further purified by centrifugation at 59,727 ×g (15 hours, 4 °C, SW 32.1 Ti rotor, Optima™ L-100 XP, Beckman Coulter) in a linear (15 – 30 % ...
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bioRxiv - Neuroscience 2023Quote: ... the crude lysate was subjected to iodixanol gradient (15%, 25%, 40% and 60%) ultracentrifugation using a Type 70Ti rotor (Beckman) at 490000 × g ...
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bioRxiv - Cell Biology 2022Quote: ... 40 μl of the mixture was then transferred to an ultracentrifuge tube and centrifuged at around 100,000 g for 15 minutes at room temperature using an airfuge (Beckman Coulter). After the centrifugation ...
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Faa1 membrane binding drives positive feedback in autophagosome biogenesis via fatty acid activationbioRxiv - Biochemistry 2023Quote: ... The reaction was centrifuged at 68 000 rpm for 15 min at 22 °C (Beckman-Coulter Optima MAX, TLA100 rotor). The supernatant and pellet were loaded separately on an SDS/polyacrylamide gel in equal amounts ...
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bioRxiv - Neuroscience 2022Quote: ... AAV was purified from the resulting lysate using an iodixanol step gradient containing 15, 25, 40 and 60% iodixanol (Batool et al., 2021) in optiseal tubes (Beckman) followed by centrifugation at 350,000 xg using a Type 70 Ti ultracentrifuge rotor (Beckman) ...
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bioRxiv - Molecular Biology 2022Quote: ... and centrifuged at 15 000 RPM for 1 minute using an AllegraTM X-22R Centriuge (Beckman Coulter™, California, USA) to ensure all reagents were concentrated at the bottom of the wells ...
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bioRxiv - Pathology 2023Quote: ... Ligated cDNAs were amplified following 15 PCR cycles and PCR products were purified using AMPure XP Beads (Beckman Coulter Genomics). Libraries were validated using a Bioanalyzer on a DNA1000 chip (Agilent ...
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bioRxiv - Microbiology 2023Quote: ... followed by downstream RNA extraction procedures as per manufacturer’s instructions including a DNase treatment step at 37°C for 15 min (Beckman Coulter).
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bioRxiv - Microbiology 2024Quote: ... About 0.7 ml of combined pellet suspension from all ultracentrifuge tubes were loaded on 15–50% linear gradient of sucrose made in PBS and spun in SW41 (Beckman) rotor at 80,000 × g for 1 h at 4 °C ...
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bioRxiv - Cell Biology 2024Quote: ... and the post-nuclear supernatant was then centrifuged at 49,000 RPM for 15 min in an TLA-55 ultracentrifuge (Beckman Coulter). The supernatant (cytosol fraction ...
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bioRxiv - Biophysics 2024Quote: ... 100 μL of Origami-R1-R2 was placed on top of a quasi-linear glycerol gradient (15–45%, in 1× TE-Mg2+ buffer) in a 0.8 mL Open-Top Thinwall Ultra-Clear tube (Beckman Coulter) and spun in an SW 55 Ti rotor (Beckman Coulter ...
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bioRxiv - Cell Biology 2024Quote: ... 0.5 ml of the dialysate was overlayed onto a 5-30% sucrose gradient made in dialysis buffer and resolved by centrifuging the gradients overnight (15-16h) at 168,000xg in a SW40 rotor (Beckman Coulter). The gradient was manually fractionated into 0.5 ml fractions and a small sample from each fraction was run on an SDS-PAGE gel and stained with Sypro Ruby to visualize protein bands ...
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bioRxiv - Molecular Biology 2022Quote: ... Samples were tagged using barcodes NB01 to NB05 of the Native Barcoding Expansion 1-12 PCR-free kit (EXP-NBD104, ONT) and purified using Agencourt AMPure XP beads (Beckman Coulter). The barcoded samples were quantified using a Qubit fluorometer and pooled equimolarly to reach 700 ng in 65 μL ...
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bioRxiv - Biochemistry 2020Quote: ... The equivalent of 200 µl of cell extract with an OD260 of 12 was loaded onto a 10–50% sucrose gradient and centrifuged in a SW41 rotor (Beckman Coulter) at 39,000 rpm for 2.45 h at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... mAb (12-1401-81, eBioscience) and then subjected to analysis with a FACS Aria (Becton Dickinson) or CytoFLEX S (Beckman Coulter).
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bioRxiv - Cell Biology 2021Quote: ... supplemented with 300 mM sodium carbonate for a total of 1.6 mL in a 12 mL ultracentrifuge tube (Beckman Coulter, #343778). Approximately ...
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bioRxiv - Molecular Biology 2023Quote: ... Every three days for 12 days the cells were split and the percentage of GFP- cells was determined using flow cytometry (CyAn ADP, Beckman Coulter). The percentage of GFP+ cells was determined using FlowJo v10 (FlowJo ...
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Novel RNA-binding protein YebC enhances translation of proline-rich amino acid stretches in bacteriabioRxiv - Molecular Biology 2024Quote: ... were prepared and cooled on ice. The 10-50% sucrose gradients (vol. = 12 mL) were formed in SW40 ultracentrifuge tubes (Beckman Coulter) using Gradient Station (Biocomp) ...
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bioRxiv - Cell Biology 2024Quote: Lung nonparenchymal cells isolated from WT mice with and without LPS treatment (n=5/group) were stained as previously described (18) and analyzed using a CytoFlex (Beckman Coulter, Brea, CA). Dead cells were excluded using Live/Dead Near IR (Invitrogen ...
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bioRxiv - Neuroscience 2020Quote: ... Tubes were capped then centrifuged for 15 minutes at 30,000xg in a JLA 16.250 fixed angle rotor (Beckman-Coulter, Brea, CA) at 4°C using a Beckman Avanti JE centrifuge ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Populations were transferred twice a week by diluting 15% of the culture into 800 µL of fresh medium using a liquid-handling robot (Biomek NXP Span-8; Beckman Coulter). At each transfer ...
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bioRxiv - Microbiology 2021Quote: ... starting with 50% and topping off with 15% for a total of 11 mL was added to thin wall polypropylene ultracentrifuge tubes (Beckman Coulter). The tubes were immediately frozen and stored at -80 °C ...
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bioRxiv - Cell Biology 2020Quote: ... The first PCR reaction was performed for 15 cycles with 60 °C annealing and 30 s elongation and then purified with AMPure XP PCR beads (Beckman Coulter). The second PCR was performed for 15 cycles for wild type-specific and for 21 cycles for tag-specific amplicons respectively using 60°C annealing and 30 s elongation ...
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bioRxiv - Biochemistry 2021Quote: ... The reaction mixtures were incubated at room temperature for 15 min followed by centrifugation at 36000 x g using Optima TL benchtop ultracentrifuge (Beckman Coulter) for 15 min at 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... sucrose gradient and the RCII complexes were separated by ultracentrifugation (40,000×g,15 h) using an Optima XPN-90 Ultracentrifuge (Beckman Coulter, USA). The separated RCII/PSI fraction was collected and then washed repeatedly using the glycerol- and DM-free elution buffer and concentrated to 0.2 mg Chl/ml which corresponded to approximately 0.6 mg protein/ml.
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bioRxiv - Cell Biology 2022Quote: ... Thromboplastins and Fibrins the plasma was centrifuge at 4700Xg for 15 mins followed by high-speed centrifugation (Beckman Avanti J-26XP) at 12,000 x g for 25mins and filtered using a 0.22μm filter (Ultident Scientific ...
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bioRxiv - Molecular Biology 2020Quote: ... The target library derived from the oocyte RNA polymerase II transcripts was PCR-amplified (15 cycles for P14 WT, 16 cycles for P14 Tbpl2−/− mutant) and purified using AMPure beads (Beckman Coulter) to remove short PCR artifacts (< 200bp ...
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bioRxiv - Immunology 2021Quote: ... The PBMC were resuspended in 1 mL cold PBS+BSA per 15 mL whole blood processed and counted with a ViCell (Beckman Coulter) using VersaLyse reagent (Beckman Coulter #A09777 ...
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bioRxiv - Genomics 2022Quote: ... was added to each reaction and incubated for 15 minutes at 37 °C followed by 15 minutes at 80 °C and the samples were purified using 1x AMPure XP beads (Beckman Coulter). For the Panhandle protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... and 1U of FastAP (TFS, EF0654) for 15 min at 37 °C and purified using 1.2× SPRIselect beads (Beckman Coulter, B23318). The inDrops-v1.1 libraries were digested with 40U of ExoI and 1U of FastAP for 15 min at 37 °C ...
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bioRxiv - Genomics 2024Quote: ... c) increasing PCR cycles (15 in total) and eliminating primer dimers prior to sequencing (Agencourt AMPure XP magnetic beads, Beckman Coulter). Primary PDAC were sequenced on the NovaSeq6000 ...
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bioRxiv - Biochemistry 2023Quote: ... Mitochondrial crude was pelleted by centrifugation at 9500 ×g (4 °C, 15 min) using the Optima XE-90 Ultracentrifuge with a Ti-45 rotor (Beckman Coulter). The final pellet was resuspended in 2 mL of Resuspension buffer (250 mM sucrose ...
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bioRxiv - Biochemistry 2023Quote: ... then centrifuged at 27,000 gmax for 15 min at 4°C (Optima XPN-100 Ultracentrifuge, Beckman Coulter, with SW60 Ti rotor). Resuspension of the pellet (sub-mitochondrial particles ...