Labshake search
Citations for Beckman :
451 - 500 of 1970 citations for Ethyl 2 pyrrolidin 2 yl 2 3 dihydro 1 3 thiazole 4 carboxylate hydrochloride since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... All experiments in this study were performed using a 3-laser CytoFLEX Flow Cytometer (Beckman Coulter, Brea, CA, USA) and operated using the CytExpert Software v2.4.0.28 (Beckman Coulter) ...
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bioRxiv - Cell Biology 2020Quote: ... The harvested sEVs were resuspended with 3 mL double filtered PBS and centrifuged with MLA-55 rotor (Beckman Coulter) under 100,000 × g at 4 °C for 70 mins to remove non-EV contaminants ...
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bioRxiv - Neuroscience 2022Quote: ... myelin was layered onto 3 mL ice cold 0.85 M sucrose solution in a 10 mL ultracentrifuge tube (Beckman) before centrifugation at 75 000 x g for 30 min at 4 °C on a Beckman Ultracentrifuge with MLA-55 rotor (acceleration 7 ...
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bioRxiv - Microbiology 2023Quote: ... and RNA were purified using 3 volume of absolute isopropanol and 1.8 volume of Agencourt RNAClean XP beads (Beckman). RNA was eluted with 6 µL of water ...
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bioRxiv - Plant Biology 2024Quote: ... and centrifuged for 3 h at 260,000 g using an SW 55 Ti swinging-bucket rotor (Beckman Coulter, USA). The final supernatant was discarded ...
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bioRxiv - Cell Biology 2023Quote: ... 0.01% Sodium Azide in PBS and measured by recording 3 × 104 events using CytoFLEX and Gallios cytometers (Beckman Coulter). The resulting data were analysed using FlowJo v10 (TreeStar) ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were treated with 20nM of gemcitabine and cells were counted 3 days later using the Z1 Coulter (Beckman).
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bioRxiv - Bioengineering 2023Quote: ... MB concentration and number- and volume-weighted size distributions were measured using a Multisizer 3 Coulter Counter (Beckman Coulter). MB concentration (𝑐i ...
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Syntaxin-2 inhibits excessive phagocytic uptake but promotes phagolysosomal clearance in macrophagesbioRxiv - Cell Biology 2024Quote: ... followed by sequential topping with 2 mL each of 35, 25, and 10% sucrose (all prepared in 3 mM imidazole, pH 7.4) in SW40 tubes (344060, Beckman). The samples were then centrifuged at 100,000 g for 1 hr at 4°C using a SW40Ti rotor (Beckman) ...
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bioRxiv - Bioengineering 2024Quote: ... the distribution of microbubble diameter and concentration was assessed using a Coulter counter (Multisizer 3; Beckman Coulter, Fullerton, California). T1 mapping MRI sequences were used to guided sonication targeting ...
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bioRxiv - Microbiology 2020Quote: ... and overlaid with 6 ml of 30% and 3 ml of 5% sucrose (w/v) followed by centrifugation at 32,000 rpm in SW41 rotor (Beckman Coulter) for 20 h ...
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bioRxiv - Molecular Biology 2020Quote: ... Debris was clarified by centrifuging at 18,000 ×g followed by fractionation on a discontinuous sucrose gradient (10-50 %) by ultracentrifugation at 39,000 rpm for 3 h in an SW41-Ti rotor (Beckman coulter). Monosome fractions were collected by pumping of Fluorinert FC-40 and using a fraction collector by measuring UV trace ...
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bioRxiv - Molecular Biology 2020Quote: ... The gradient was formed by adding 3 mL of the iodixanol solutions to a 17 mL polypropylene tube (Beckman Coulter). Ultracentrifugation was performed using a SW Type 32.1 Ti rotor in Optima XPN-100 Ultracentrifuge (Beckman Coulter ...
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bioRxiv - Cancer Biology 2020Quote: The barcode split of the cDNA amplification reaction (from 10x Single Cell 3’ v3 Step 2.2) was purified via 1.2x SPRI Select (Beckman Coulter #B23317). cDNA products were eluted in 40 µL of EB ...
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bioRxiv - Neuroscience 2020Quote: ... reverse 5’-CTTA TTAGTCAGTCAGAAACAAC-3’).ThePCR amplicon was purified using RNAse-free SPRI beads (Agencourt RNAclean XP, Beckman-Coulter A63987) and then used for in vitro transcription with the HiScribe T7 ARCA mRNA Kit (with tailing ...
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bioRxiv - Microbiology 2021Quote: 3 mL of pseudoparticles were pelleted using a TLA-55 rotor with an Optima-MAX-E ultracentrifuge (Beckman Coulter, Inc.) for 2 hours at 42,000 rpm at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... Supernatants (3 ml) were loaded on the top of step gradients preformed in polyallomer tubes (14 by 89 mm, Beckman), containing 2 ml each of 1.82 ...
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bioRxiv - Molecular Biology 2020Quote: ... 1.3 M, 2.5 M) as indicated in the method (Vella et al., 2017) in ultra-clear SW40Ti tubes (Beckman Coulter). The sucrose gradients were centrifuged at 200,000 x g avg at 4°C for 173 min using a SW40Ti rotor (Beckman Coulter) ...
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bioRxiv - Microbiology 2020Quote: ... was overlaid on 25% sucrose in PBS and centrifuged at 26,000 rpm for 3 h at 4C using SW28 rotor (Beckman Coulter). Pellets were dissolved in PBS overnight at 4C and 1/3 of used for RNA was extraction using Macherey Nagel Virus RNA isolation kit without carrier RNA according to the manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2020Quote: ... Samples are visualized on Cytoflex S, 3 lasers (488 nm, 405 nm and 638 nm) and 13 detectors (Beckman Coulter). Live cells were gated based on side scatter and forward scatter ...
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bioRxiv - Molecular Biology 2020Quote: ... and the cleared supernatant was loaded on a 3 to 30% iodixanol gradient (OptiPrep) and ultracentrifuged for 5h at 36,000 rpm in an SW60 rotor (Beckman Coulter). Fractions were collected manually through aspiration and RNA or proteins were isolated for further analysis.
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bioRxiv - Microbiology 2020Quote: ... The filtered supernatant was then spun through a 20% sucrose cushion at 48,000 rpm for 3 hours in a SW55Ti rotor (Beckman Coulter). The SVP pellets were resuspended in a 4X non-reducing buffer (Alfa Aesar ...
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bioRxiv - Microbiology 2021Quote: ... 60% w/v) and centrifuged at 64,000 x g for 3 hours in A27-8×50 mL rotor (Beckman Coulter). The visible virus bands were collected ...
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bioRxiv - Molecular Biology 2023Quote: ... The reactions were then layered on 100 μL of 32% sucrose cushion and centrifuged at 42,800 rpm for 3 hours in a Beckman TLA 100 rotor using the Optima-Max TL ultracentrifuge (Beckman Coulter). After centrifugation ...
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bioRxiv - Microbiology 2022Quote: ... HUVECs or LECs were infected for 3 days and the GFP positive population was quantified by CytoFLEX S (Beckman Coulter). Here ...
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bioRxiv - Bioengineering 2024Quote: ... The sample tubes were then centrifuged at 347,000 × g and 20°C for 3 hours using a Type 70 Ti rotor (Beckman Coulter). 1-mL fractions were collected from the top of the tube ...
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bioRxiv - Microbiology 2024Quote: ... The flow cytometry analysis of the bacterial cells present in the suspension was performed using a Cytoflex flow cytometer (CytoFLEX 3; Beckman) as previously described (Supplementary Fig ...
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bioRxiv - Cancer Biology 2024Quote: ... The fragments were repaired the 3’and 5’overhangs using End repair mix (component of SureselectXT) and purified using Agencourt AMPure XP Beads (Beckman). The purified fragments were added with ‘A’ tail using A tailing Mix (component of SureSelectXT ...
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bioRxiv - Microbiology 2022Quote: ... The sample-containing tube was spun at 347,000 × g and 20°C for 3 hours using Type 70 Ti rotor (Beckman Coulter). 1-mL fractions were collected from the top of the tube ...
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bioRxiv - Cell Biology 2022Quote: ... 4°C for 1 hour (JA 25.5 rotor, Beckman Coulter). The supernatant was kept and clarified by a 0.45 µM syringe filter (Pall Corporation) ...
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bioRxiv - Genetics 2020Quote: ... PCR products were pooled by region of interest using 2-3 μL of each reaction and the resulting pools of products were purified using a 1X Ampure XP cleanup (Beckman Coulter). Purified libraries were pooled and submitted for paired-end 150 cycle sequencing on an Illumina MiSeq ...
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bioRxiv - Genomics 2021Quote: ... Cells were washed with 25 mm supplemented HBSS before depleting the sample of unnucleated cells using IOTest 3 Lysing solution (Beckman Coulter) following provider instructions ...
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bioRxiv - Microbiology 2021Quote: ... triplicate spore preparations were made in 3 ml of 20% glycerol and suspended in Isoton II (Beckman Coulter, Brea, CA, USA). The particle volume was then measured for a minimum of 100 000 particles per sample using a Multisizer 4e Coulter counter (Beckman Coulter ...
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bioRxiv - Cell Biology 2020Quote: ... with densities ranging from 1.10 to 1.15 were pooled and washed in PBS (Beckman Ti50, 3 h at 42 000 rpm).
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bioRxiv - Microbiology 2022Quote: A 3 ml volume of pseudoparticles was pelleted using a TLA-55 rotor with an Optima-MAX-E ultracentrifuge (Beckman Coulter) for 2 hours at 42,000 rpm at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... 3 ml of 50% and 3 ml of 40% sucrose) in Ultra-Clear Beckman tubes and centrifuged at 26000 rpm (SW32Ti rotor, Beckman Coulter) for 1 h at 4°C ...
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bioRxiv - Biochemistry 2020Quote: ... and pelleted for 3 min in a swing-out rotor at 1000 rpm in a tabletop centrifuge (Allegra X-30R, Beckman Coulter). The supernatant was aspirated and cells were suspended in the remaining liquid to 1 × 105 – 1 × 107 cells/100 μl ...
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bioRxiv - Genomics 2020Quote: ... We carried out the first round of PCR for 3 cycles and purified the reactions with Ampure XP beads (Beckman-Coulter). We then carried out a second round of PCR with primers targeting the P5 and P7 sequences only ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were harvested after 3 days and counted using a Z2 Coulter Particle Count and Size Analyzer (Beckman Coulter, Brea, CA).
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bioRxiv - Cancer Biology 2024Quote: ... The percentage of fluorescence-positive cells was determined initially (baseline reference, passage 0, 3 days after transduction) and all following passages using the CytoFLEX Flow Cytometer (Beckman Coulter). In each passage ...
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bioRxiv - Cell Biology 2023Quote: ... 30% Percoll (v/v)] followed by 3 mL of MRB in a 13 mL ultracentrifuge tube (Beckman Instruments, Inc, CA 331372) and spun at 95,000 x g for 45 min in a TH641 rotor ...
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bioRxiv - Genetics 2023Quote: ... the cell pellet was resuspended in PBS supplemented with 2 % FCS and nucleated cells were counted in methylene blue with 3 % acetic acid on a Vi-Cell XR cell viability counter (Beckman Coulter). 10 × 106 bone marrow cells were resuspended in 200 μl of PBS supplemented with 2 % FCS containing the following antibody solution ...
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bioRxiv - Molecular Biology 2024Quote: ... bottom layer was made by diluting OptiPrep™ solution with clarified HM or digesta sample (2.25 mL OptiPrep™ + 0.75 mL sample = 3 mL) into a 5 mL open-top thin wall ultra-clear tube (Beckman Coulter). A discontinuous gradient was made with 40% (w/v) ...
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bioRxiv - Bioengineering 2022Quote: ... Supernatant (33 mL) was placed on a 3 mL 25% (w/v) sucrose pad in SW28 tube (Beckman, Brea, CA, USA) and centrifuged at 24,000 rpm ...
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bioRxiv - Cancer Biology 2023Quote: Counting of CB CD34+ cells after 3 days of ex vivo co-culture was accurately obtained with flow-count fluorosphere beads (Beckman Coulter), in combination with the following antibodies ...
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bioRxiv - Immunology 2024Quote: ... Fluorescence signals and TruCount beads were acquired on a 3-laser CytoFLEX using Cytexpert software 2.3 (Beckman Coulter, Brea, CA, USA). Instrument quality control and standardization were performed daily using CytoFLEX Daily QC Fluorospheres (Beckman Coulter #B53230).
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bioRxiv - Pathology 2024Quote: ... with primers located in the U3 region of the 5’ and 3’ LTRs (Figure S1) and purified using “1X AmpureXP beads” (Beckman Coulter). ONT (Oxford nanopore technologies ...
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bioRxiv - Cancer Biology 2024Quote: ... washed 3 times with chilled phosphate-buffered saline (PBS) and analyzed by flow cytometry (CytoFLex, Beckman Coulter Inc., Brea, CA, USA)[39 ...
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bioRxiv - Molecular Biology 2020Quote: ... MgCl2 5mM and Tris-Cl 50mM pH 7.4) was added into 3-mL polycarbonate tube for an ultracentrifuge TL100.3 rotor (Beckman Coulter, Brea, CA, USA). The KCl-adjusted ribosome-containing solution was carefully added above the sucrose cushion and tubes were balanced by weight using buffer B (sucrose 250mM ...
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bioRxiv - Molecular Biology 2021Quote: ... Produced amplicons of 3-144 µm eukaryotes and Imitervirales were purified with the magnetic beads (Agencourt AMPure XP beads, Beckman Coulter, Inc.). Finial DNA production was dissolved in 27.5 µL ultrapure water ...