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Citations for Beckman :
451 - 500 of 1840 citations for 2 1 1 Diethoxy 2 methyl propyl 4' Nitrophenyl Carbonate d6 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... 1 mL reaction buffer containing substrates (25 μM-0.16 mM NADH and 2 mM pyruvate) was added to the cuvette of a spectrophotometer (DU® 700, Beckman Coulter). The reaction was started by adding cell lysate ...
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bioRxiv - Cancer Biology 2024Quote: ... The exosome pellet was resuspended in 35 ml of 0.2 μm-filtered 1x PBS and collected by ultracentrifugation at 100,000 × g for 2 h (45ti rotor, Optima L-100 XP Centrifuge, Beckman Coulter, USA). Exosome pellets were resuspended in 200-300 µL of 0.2 μm-filtered 1x PBS and stored at -80°C ...
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bioRxiv - Molecular Biology 2020Quote: ... All amplified cDNA was purified using a 1:1 ratio of AMPure XP beads (Beckman Coulter, USA), and resuspended in 25 ml ...
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bioRxiv - Genomics 2021Quote: ... and short fragments were removed by mixing 1:1 by volume with AMPureXP magnetic beads (Beckman Coulter) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... and 1 mM dithiothreitol) and spun for 1 h at 100,000 rpm using TLA-110 rotor (Beckman) at 4 °C ...
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bioRxiv - Plant Biology 2023Quote: ... Samples were purified with a 1:1 ratio of AMPure XP SPRI beads (Beckman Coulter – cat#A63880) and resuspended in 51μl EB ...
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bioRxiv - Developmental Biology 2023Quote: ... Pre-amplified cDNA was purified with SPRI AMPure XP beads (Beckman, sample to beads ratio 1:1) and eluted in 15 μl Buffer EB (QIAGEN) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Digested-ligated products were purified using 1:5:1 ratio of AMPure XP beads (Beckman Coulter, USA). Size selection was performed using a BluePippin to retain total fragment sizes of 250-500bp and were purified using the QIAquick Gel Extraction Kit (Qiagen ...
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bioRxiv - Molecular Biology 2022Quote: ... 4°C (Beckman Coulter). The pellet containing lentiviral particles was suspended in the residual medium ...
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bioRxiv - Genomics 2020Quote: ... 4°C (Beckman Coulter). Supernatant was loaded onto 5 ml HisTrap FF columns (GE Healthcare ...
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bioRxiv - Molecular Biology 2020Quote: ... 4°C (Beckman Coulter). Clarified lysates were poured several times over 0.5-1 ml Streptactin resin (IBA ...
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bioRxiv - Cancer Biology 2023Quote: ... 4°C (Beckman Coulter). The pellet containing lentiviral particles was suspended in the residual medium ...
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bioRxiv - Neuroscience 2020Quote: ... Final 15 mL of solution was mixed by inverting the tube 10x and carefully pipetted into 2 ultracentrifuge tubes (Beckman Coulter #344059) onto the isolation buffer cushion (5 mL ...
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bioRxiv - Microbiology 2020Quote: ... were pelleted through a 20% (wt/vol) sucrose cushion at 25,000 rpm for 2 h in an SW41 rotor (Beckman Coulter, Tokyo, Japan). Then ...
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bioRxiv - Neuroscience 2022Quote: ... which was then sealed and placed into a hematocrit spinner (2 minutes at 13, 700 g; StatSpin CritSpin Microhematocrit Centrifuge, Beckman Coulter, Inc). Measurements were taken to calculate percent (% ...
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bioRxiv - Cancer Biology 2022Quote: ... The cell pellet was disaggregated and incubated with 2 μL of anti-pan cytokeratin-FITC (Beckman Coulter, Cat. No. IM2356U, Clone J1B3). A separate tube containing cells unstained for surface E-Cadherin was incubated after permeabilization with E-Cadherin antibody- (R&D Cat ...
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SLC15A4 favors inflammasome function via mTORC1 signaling and autophagy restraint in dendritic cellsbioRxiv - Immunology 2022Quote: ... Cells were finally washed in PBS with 0.5% BSA and 2 mM EDTA and analyzed by flow cytometry using a CytoFLEX (Beckman Coulter, Indianapolis, IN) and FlowJo software (BD Biosciences) ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Clarified supernatants were layered over 2 ml of a 25% (w/v) sucrose solution in 13.2 ml Thinwall Polypropylene SW41 Ti ultracentrifuge tubes (Beckman-Coulter, Brea, USA) and made up to a total volume of 10 ml with PBS without calcium chloride or magnesium chloride (PBS[-]) ...
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bioRxiv - Molecular Biology 2020Quote: ... was subjected to centrifugation at 100,000g for 2 h in an Optima MAX-XP benchtop ultracentrifuge with a TLA-100.3 rotor (Beckman Coulter, Inc., Brea, CA). Pellets were resuspended in 100 µl PBS and stored at −80 °C.
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bioRxiv - Neuroscience 2020Quote: ... Homogenates were layered on top of 1 ml of 0.85 M sucrose in 2 mM EGTA and centrifuged at 75,000 g for 30 min in a Beckman FW-27 rotor (Beckman, Fullerton, CA, USA). This step was repeat twice ...
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bioRxiv - Microbiology 2021Quote: ... soil was thoroughly mixed with 20 ml of 0.01 M CaCl2 for 30 min on a Elmi Intelli-Mixer RM-2 (Elmi Tech Ltd, Latvia) and subjected to centrifugation in an Allegra X-22R centrifuge (Beckman Coulter, USA) at 3900 rcf for 20 min and to remove inorganic S (Dedourge et al. ...
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bioRxiv - Microbiology 2023Quote: ... the prepared DNA library was size-selected on a 2% agarose gel (410–470 bp) using the Agencourt SPRI system to reduce mitochondrial contamination (Beckman Coulter, Inc.). After library prep ...
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bioRxiv - Biophysics 2023Quote: ... The virions were concentrated by ultracentrifugation through a 20% (w/v) sucrose cushion (2 h at 28,000 rpm in a SW32 rotor [Beckman Coulter Life Sciences]). The pellet was resuspended in PBS ...
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bioRxiv - Genetics 2023Quote: ... in-house VLPs were produced using the same protocol followed by ultracentrifugation at 24,000 RPM for 2 hours (SW 28 Beckman swinging bucket rotor) to concentrate the VLPs and avoid diluting the HSPCs culture media ...
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bioRxiv - Plant Biology 2020Quote: ... The constructed libraries were size-selected using 1:1 volume of AMPure XP beads (Beckman Coulter, Brea CA). Size and quality of libraries were examined using Bioanalyzer 2100 (Agilent ...
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bioRxiv - Microbiology 2020Quote: Amplified cDNA was purified using a 1:1 concentration of AMPure XP beads (Beckman Coulter, Brea, CA, USA) and eluted in 48μL of water ...
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bioRxiv - Microbiology 2020Quote: Amplified cDNA was purified using a 1:1 concentration of AMPure XP beads (Beckman Coulter, Brea, CA, USA) and eluted in 48μL of water ...
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Induction of Dopaminergic Neurons for Neuronal Subtype-Specific Modeling of Psychiatric Disease RiskbioRxiv - Neuroscience 2021Quote: ... Libraries were subsequently purified using a 1:1 volumetric ratio of SPRI beads (Beckman Coulter Life Sciences, #B23318) to remove primer dimers and enrich for a target library size of ~300bp ...
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bioRxiv - Microbiology 2022Quote: ... solution was added 1:1 to EV suspensions and transferred to an MLS-50 rotor tube (Beckman; 344057). Iodixanol stock was then diluted in 0.25 M sucrose-Tris buffer to make 10% and 20% iodixanol solutions ...
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bioRxiv - Microbiology 2021Quote: Amplified cDNA was purified using a 1:1 concentration of AMPure XP beads (Beckman Coulter, Brea, CA, USA) and eluted in 30μL of water ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Amplified cDNA was purified using a 1:1 concentration of AMPure XP beads (Beckman Coulter, Brea, CA, USA) and eluted in 30 μL of water ...
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bioRxiv - Genomics 2020Quote: ... We purified PCR products in a 1:1 ratio using AmpureXP purification beads (Beckman Coulter, High Wycombe, UK), and quantified the purified amplicon using the Qubit dsDNA High Sensitivity assay kit on a Qubit 4.0 instrument (Life Technologies) ...
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bioRxiv - Immunology 2023Quote: ... and intracellularly stained with the Fluorescein isothiocyanate-labeled KC57 (anti-HIV-1 p24) antibody (1:200) (Beckman Coulter) or a mixture of both antibodies ...
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bioRxiv - Microbiology 2023Quote: ... 100 µl/well of a 1:1 mix of methanol:acetonitrile were added using the Biomek FXP (Beckman Coulter) liquid handling system ...
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bioRxiv - Cancer Biology 2020Quote: ... and GlyA PE (1:200, Beckman Coulter). Finally ...
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bioRxiv - Cancer Biology 2020Quote: ... CD41 PE-Cy5 (1:200, Beckman Coulter), GlyA PE (1:100 ...
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bioRxiv - Cancer Biology 2020Quote: ... CD41 PE-Cy5 (1:200, Beckman Coulter), GlyA PE (1:100 ...
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bioRxiv - Cancer Biology 2020Quote: ... CD41 PE-Cy5 (1:200, Beckman Coulter), GlyA PE (1:100 ...
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bioRxiv - Cancer Biology 2020Quote: ... CD41 PE-Cy5 (1:200, Beckman Coulter) and GlyA PE (1:200 ...
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bioRxiv - Immunology 2021Quote: ... CD103 (clone HML-1, Beckman Coulter, IM1856U). Cells were stained at antibody dilutions of 1:30 ...
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bioRxiv - Immunology 2024Quote: ... PD1 (PE-Cy5.5, 1/320, Beckman Coulter), IL-4 (PerCP-Cy5.5 ...
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bioRxiv - Microbiology 2020Quote: ... The clarified supernatant was overlaid onto a 20% (w/v) sucrose cushion in Tris-NaCl-EDTA buffer and centrifuged at 134,000 g for 2 hours (Beckman Coulter 30 Ti rotor). The resulting viral pellet was suspended in VCM by repeat pipetting and aliquots stored in cryovials at minus 70°C until needed ...
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bioRxiv - Cell Biology 2020Quote: ... 3 μM or 10 μM from a 2 mM or 10 mM stock solution using a Biomek FX with 384-well head (Beckman Coulter, Indianapolis, IN). The DMSO concentration was kept below or at 0.5% v/v ...
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bioRxiv - Physiology 2022Quote: ... the library preparation step followed the CEL-Seq 2 protocol85: the RT product was digested by ExoI and HinFI and purified using AMpure XP beads (Beckman Coulter™, USA). Then ...
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bioRxiv - Microbiology 2024Quote: ... then the colonies were diluted and inoculated into the Neg MIC EN-2J panel containing β-lactams listed in Table 2 and incubated in MicroScan Walkway40 Plus (Beckman Coulter Inc.). Breakpoint criteria for susceptible (S) ...
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bioRxiv - Molecular Biology 2024Quote: ... Each compound was dissolved in DMSO at a concentration of 2 mM and distributed into an Echo Qualified 384-Well Polypropylene Microplates (Beckman Coulter, 001-14615). Using an Echo 655 Liquid Handler ...
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bioRxiv - Immunology 2021Quote: ... supplemented with protease inhibitor cocktail and 1 mM TCEP and centrifuged at 40,000 RPM for 1 h (45 Ti fixed-angle rotor, Beckman). Supernatant was incubated with amylose beads pre-equilibrated in the same buffer for 1 h at 4 °C and loaded onto a gravity flow column ...
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bioRxiv - Synthetic Biology 2020Quote: ... Reverse transcription reactions were then purified using Agencourt Ampure XP beads at a 1:1 dilution (Beckman-Coulter, A63881). Some portion of the eluent ...
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bioRxiv - Molecular Biology 2020Quote: ... The cDNA from each reaction was purified using a 1:1 ratio of AMPure XP beads (Beckman Coulter, USA). 5ml cDNA was amplified with Q5 High-Fidelity DNA Polymerase (NEB ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Amplified PCR product was purified using a 1:1 concentration of AMPure XP beads (Beckman Coulter, Brea, CA, USA) and eluted in 30 μL of water ...