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Citations for Beckman :
451 - 500 of 1989 citations for 1 5 Bis 2 2 methyl 1 oxoallyl oxy ethyl dihydrogen benzene 1 2 4 5 tetracarboxylate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... The donor-derived LT-HSC cell populations were sorted according to the gating strategy in Extended data Figure 2 using MoFlo Astrios Cell Sorter (Beckman Coulter). LT-HSC cells were directly sorted into 50μl RNA extraction buffer (ARCTURUS PicoPure RNA Isolation Kit (Applied Biosystems ...
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bioRxiv - Cancer Biology 2022Quote: ... 10 nM leptomycin b, vehicle control or library compounds (Supplementary Tables 2, 3) were added using an Echo liquid handler (Labcyte/Beckman Coulter). Cells were incubated for a further 24 h then fixed in 4% paraformaldehyde and stained with DAPI and Alexa Fluor 488 Phalloidin (ThermoFisher) ...
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bioRxiv - Microbiology 2022Quote: ... Washed pellets were resuspended in 2 mL 1x PBS and fluorescence was calculated using a LSR II cell analyzer (Beckman Coulter). Two biological replicates of 50,000 cell counts were analyzed with FlowJo ...
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bioRxiv - Neuroscience 2020Quote: ... the homogenized solution was added onto a sucrose cushion (2 ml) in a 10 ml Ultra-Clear centrifuge tube (Beckman Coulter). Separation of nuclei from the tissue was done by centrifugation (26.500xg at 4°C for 1.5 h ...
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bioRxiv - Microbiology 2022Quote: ... filtered supernatant was underlaid with 20% sucrose PBS using a pipetting needle and centrifuged at 39 000 rpm for 2 h using an SW-41 rotor (Beckman Coulter). The supernatant and sucrose solution were carefully removed ...
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bioRxiv - Microbiology 2022Quote: ... The virus suspension was pelleted through a 40% sucrose cushion by centrifugation for 2 h at 40,000 rpm in a SW55 Ti rotor (Beckman, Fullerton, CA) at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... Samples were incubated for 2 h at 16°C and double stranded cDNA was purified using AMPure XP beads (Beckman Coulter). For testing transcription activity of predicted genes ...
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bioRxiv - Cell Biology 2021Quote: ... to remove cellular debris and purified by ultracentrifugation over a 25% sucrose cushion (28.000 rpm for 2 hours; Beckman Coulter ultracentrifuge).
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bioRxiv - Cancer Biology 2021Quote: ... Cells were treated with 3 different doses of the conventional chemotherapy for 2 days and cell viability was assessed using a Vi-cell XR Viability Analyzer (Beckman Coulter); 6 replicates were performed for each cell line and each dose.
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bioRxiv - Genetics 2020Quote: ... Timepoints were measured every 2-3 days by flow cytometry analysis of >7,000 cells (either a BD Accuri C6 or Beckman Coulter CytoFLEX). Data was analyzed using Cytoflow (https://github.com/bpteague/cytoflow ...
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bioRxiv - Genetics 2020Quote: ... Reverse crosslinking reactions were incubated 2 h at 65°C and cleaned using Agencourt Ampure XP SPRI beads (Cat #A63880, Beckman Coulter) with a 2x bead:sample ratio ...
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bioRxiv - Immunology 2021Quote: ... Beckman Coulter Access SARS-CoV-2 IgG assays (Brea, CA, USA) targeting the RBD were performed on UniCel DxI 800 Access Immunoassay System (Beckman Coulter). Index value threshold for positivity was 1 as recommended ...
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bioRxiv - Immunology 2020Quote: ... erythrocytes in 2 μl blood (collected from the tail tip) were counted using a Z2 Coulter Particle count and size analyser (Beckman Coulter). We determined the healthy range in uninfected C57Bl/6J mice housed in our facility to be 8.8 - 10.5*109 erythrocytes*ml−1 ...
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bioRxiv - Microbiology 2021Quote: ... and either aliquoted or concentrated by ultracentrifugation using 20% (w/v) sucrose cushions for 2 hours at 25,000 rpm in an SW41 or SW28 rotor (Beckman, Indianapolis, IN). Alternatively ...
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bioRxiv - Immunology 2020Quote: ... 2) first-round PCRs were set up using a Biomek FX liquid handling system with a 96- well head (Beckman Coulter); 3 ...
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bioRxiv - Genomics 2021Quote: ... samples were purified with 45 µl of Agencourt AMPure XP magnetic beads with a sample to beads ratio of 3:2 (Beckman Coulter), normalized by Qubit quantification and pooled to generate a 4 nM library ...
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bioRxiv - Cell Biology 2022Quote: ... A 12 ml 2 % step Optiprep gradient in LB ranging from 32 % to 10 % was prepared beforehand in Ultra-Clear tubes (Beckman Coulter). Samples were spun for 16 hours at 100000 g at 4 °C ...
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bioRxiv - Genomics 2024Quote: ... The crude nuclei were stained with 4,6-diamidino-2-phenylindole (DAPI) and loaded into a flow cytometer (Beckman Coulter, MoFlo XDP). Nuclei were purified by flow sorting and washed as described previously [61] ...
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bioRxiv - Neuroscience 2023Quote: ... After PCR the samples were combined into small pool of 2-6 samples and size selection was carried out by adding 0.55x volume of AMPure XP beads (Beckman Coulter, #A68831). The concentrations of purified library pools were quantified with Collibri™ Library Quantification Kit (ThermoFisher ...
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bioRxiv - Biochemistry 2024Quote: ... 50 mM Tris-HCl pH 7.4) and ultracentrifuged at 250 000 x g for 2 h (Beckman Optima TL 100 Ultracentrifuge). After ultracentrifugation ...
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bioRxiv - Microbiology 2023Quote: ... viruses were collected and concentrated by ultracentrifugation (37,000 rpm, 2 h 30, 15 °C; Beckman Type 45 Ti fixed-angle rotor). The virus titer was determined using a plaque assay.
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bioRxiv - Microbiology 2023Quote: ... the DNA monitored on 2% agarose gel was purified by means of AMPure XT beads (Beckman Coulter Genomics, Danvers, MA, USA) and quantified by Qubit (Invitrogen ...
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bioRxiv - Biophysics 2023Quote: ... and 12-fold diluted in ice-cold SCL and subjected to ultracentrifugation at 100 000 x gav for 2 h in a Ti 70 fixed-angle rotor (Beckman Coulter). The resulting pellets were resuspended in SCL and freshly used for downstream analysis and never subjected to freezing and thawing.
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bioRxiv - Biochemistry 2024Quote: ... 1 mL reaction buffer containing substrates (25 μM-0.16 mM NADH and 2 mM pyruvate) was added to the cuvette of a spectrophotometer (DU® 700, Beckman Coulter). The reaction was started by adding cell lysate ...
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bioRxiv - Cancer Biology 2024Quote: ... The exosome pellet was resuspended in 35 ml of 0.2 μm-filtered 1x PBS and collected by ultracentrifugation at 100,000 × g for 2 h (45ti rotor, Optima L-100 XP Centrifuge, Beckman Coulter, USA). Exosome pellets were resuspended in 200-300 µL of 0.2 μm-filtered 1x PBS and stored at -80°C ...
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bioRxiv - Biophysics 2022Quote: ... and the supernatant was subjected to ultracentrifugation to separate the membrane fraction (100,000g, 1 hour, 4°C using an Optima XE-90, Beckman Coulter centrifuge). Membranes were resuspended in lysis buffer supplemented with cOmplete EDTA-free protease inhibitors (Roche) ...
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bioRxiv - Cell Biology 2022Quote: ... A new supernatant was collected in a prechilled 1.5 ml ultracentrifuge tube and centrifuged at 100000 g for 1 h at 4°C in a TLA-55 rotor (Beckman Coulter). The resulting pellet was resuspended in the same isotonic buffer containing antiproteases and was frozen at -80°C together with the 100000 g supernatant ...
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bioRxiv - Systems Biology 2020Quote: ... each resultant fraction underwent separate ultracentrifugation at 100,000xg for 40 minutes at 4°C (rotor TLA 120.2) in 1 ml 11×34 mm polycarbonate uncapped centrifuge tubes (Beckman Coulter 343778). In EV-enriched pooled fraction experiments ...
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bioRxiv - Microbiology 2020Quote: ... Vesicles were obtained by centrifugation at 38,400 x g for 1 h at 4°C in an Avanti J-20XP centrifuge (Beckman Coulter, Inc). Pelleted vesicles were resuspended in 20 ml of 50 mM HEPES (pH 6.8 ...
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bioRxiv - Microbiology 2020Quote: ... cells were fixed with 4% FA in PBS for 90 min at room temperature and immunostained for intracellular HIV-1 CA for 30 min at 4°C using KC57-FITC antibody (Beckman Coulter) diluted in 0.1% Triton X-100 ...
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bioRxiv - Microbiology 2024Quote: ... The final Hcp1-VLPs particles were pelletized by ultracentrifugation at 274,000 ×g at 4°C for 1 h in a SW41 Ti rotor (Beckman Coulter, Inc). The final pellet was resuspended in PBS pH 7.4.
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bioRxiv - Cell Biology 2024Quote: ... plasma samples were thawed on ice and subjected to ultracentrifugation at 100,000 g for 1 hr or 18 hr at 4 °C (TLA-55 rotor; Optima MAX-MP Tabletop Ultracentrifuge, Beckman Coulter). Pellets were washed once in 1.0 ml PBS ...
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bioRxiv - Cell Biology 2023Quote: ... Homogenates were then transferred to a 50 ml tube and centrifuged for 10 mins at 1 000 × g at 4°C (Beckman Coulter). The resulting supernatant was then transferred to microfuge tubes for storage at -80°C as whole liver lysates or to fresh ultracentrifuge tubes for further processing.
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bioRxiv - Bioengineering 2023Quote: ... The suspension was then ultracentrifuged (58,000 × g, 1 hr., 4 °C) in an Optima MAX-XP (Beckman Coulter, Indianapolis, IN, USA) to separate residual catalyst ...
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bioRxiv - Biochemistry 2024Quote: ... Non-solubilized materials were removed by centrifuging the sample at 41,000 r.p.m for 1 h at 4 °C using rotor type 45 Ti in the OptimaTM L-100 XP Ultracentrifuge (BECKMAN COULTER). The resulting supernatant containing the solubilized LysP-GFP was incubated with Ni2+-NTA resin (Qiagen ...
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bioRxiv - Microbiology 2024Quote: ... and labeled intracellularly for the structural HIV-1 p24 protein with the anti-p24 clone KC57 antibody (30 min RT followed by 30 min 4°C, Beckman Coulter). HIV-1 RNA probing was performed using the PrimeFlow RNA Assay (ThermoFisher) ...
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Sense codon-misassociated eRF1 elicits widespread ribosome stalling and induction of quality controlbioRxiv - Molecular Biology 2024Quote: ... The mixture was layered over 900 μl of 1 M sucrose cushion 46 and ultracentrifuged at 100,000 rpm ( 543,000 × g) at 4°C for 1 h using a TLA-110 rotor and an Optima MAX-TL Ultracentrifuge (both Beckman Coulter).
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bioRxiv - Microbiology 2024Quote: ... the supernatant was filtered through 0.45-μm syringe filters and ultracentrifuged at 100,000 ×g for 1 h at 4 °C (MLA-50 fixed angle rotor, Beckman Coulter, Germany). The supernatant was discarded ...
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bioRxiv - Molecular Biology 2020Quote: ... All amplified cDNA was purified using a 1:1 ratio of AMPure XP beads (Beckman Coulter, USA), and resuspended in 25 ml ...
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bioRxiv - Genomics 2021Quote: ... and short fragments were removed by mixing 1:1 by volume with AMPureXP magnetic beads (Beckman Coulter) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... and 1 mM dithiothreitol) and spun for 1 h at 100,000 rpm using TLA-110 rotor (Beckman) at 4 °C ...
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bioRxiv - Plant Biology 2023Quote: ... Samples were purified with a 1:1 ratio of AMPure XP SPRI beads (Beckman Coulter – cat#A63880) and resuspended in 51μl EB ...
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bioRxiv - Developmental Biology 2023Quote: ... Pre-amplified cDNA was purified with SPRI AMPure XP beads (Beckman, sample to beads ratio 1:1) and eluted in 15 μl Buffer EB (QIAGEN) ...
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bioRxiv - Molecular Biology 2023Quote: ... 5 mM MgCl2) and spun in an SW41Ti rotor (Beckman) for 20 hours at 30,900 rpm ...
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bioRxiv - Neuroscience 2020Quote: ... Final 15 mL of solution was mixed by inverting the tube 10x and carefully pipetted into 2 ultracentrifuge tubes (Beckman Coulter #344059) onto the isolation buffer cushion (5 mL ...
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bioRxiv - Microbiology 2020Quote: ... were pelleted through a 20% (wt/vol) sucrose cushion at 25,000 rpm for 2 h in an SW41 rotor (Beckman Coulter, Tokyo, Japan). Then ...
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bioRxiv - Neuroscience 2022Quote: ... which was then sealed and placed into a hematocrit spinner (2 minutes at 13, 700 g; StatSpin CritSpin Microhematocrit Centrifuge, Beckman Coulter, Inc). Measurements were taken to calculate percent (% ...
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bioRxiv - Cancer Biology 2022Quote: ... The cell pellet was disaggregated and incubated with 2 μL of anti-pan cytokeratin-FITC (Beckman Coulter, Cat. No. IM2356U, Clone J1B3). A separate tube containing cells unstained for surface E-Cadherin was incubated after permeabilization with E-Cadherin antibody- (R&D Cat ...
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SLC15A4 favors inflammasome function via mTORC1 signaling and autophagy restraint in dendritic cellsbioRxiv - Immunology 2022Quote: ... Cells were finally washed in PBS with 0.5% BSA and 2 mM EDTA and analyzed by flow cytometry using a CytoFLEX (Beckman Coulter, Indianapolis, IN) and FlowJo software (BD Biosciences) ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Clarified supernatants were layered over 2 ml of a 25% (w/v) sucrose solution in 13.2 ml Thinwall Polypropylene SW41 Ti ultracentrifuge tubes (Beckman-Coulter, Brea, USA) and made up to a total volume of 10 ml with PBS without calcium chloride or magnesium chloride (PBS[-]) ...