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Citations for Diagenode :
1 - 50 of 322 citations for hsa mir 32 Real Time RT PCR Detection and U6 Calibration Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... Real Time qPCR was performed by using a real-time PCR SYBR green master mix (Diagenode). Samples were run and analyzed on a Quantstudio 5 (Applied Biosystems) ...
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bioRxiv - Cell Biology 2019Quote: ... cDNA was quantified by real-time PCR using SYBR Green Master Mix (Diagenode) on a QuantStudio 6 instrument (Themo Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... cDNA was quantified by real-time PCR using SYBR Green Master Mix (Diagenode) on a QuantStudio 6 instrument (Themo Scientific ...
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bioRxiv - Molecular Biology 2022Quote: ... cDNA was quantified by real-time PCR using SYBR Green Master Mix (Diagenode) on a Quant Studio 5 384 well plate ...
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bioRxiv - Genomics 2023Quote: ... 15μg of DNA was purified then sheared at 15kb (speed 31 and 32) with the Megaruptor3 system (Diagenode). Using SMRTbell Express Template prep kit 2.0 ...
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bioRxiv - Cancer Biology 2022Quote: ... We sonicated the samples for 32 cycles of 30 s at full intensity using a Bioruptor Pico (Diagenode, Seraing, Belgium) and pelleted cell debris by spinning at 21,130 × g for 15 min ...
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bioRxiv - Microbiology 2019Quote: ... pneumophila in the samples was detected by diagnostic PCR using primers and probes of the R-DiaLegTM kit (Diagenode, Belgium). The PCRs were prepared in a final volume of 20 μL by adding the Master Mix 5X (TaqMan Probe LC2.0 ...
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bioRxiv - Microbiology 2023Quote: ... at room temperature (RT) for 30min and then quenched with glycine 0.125M (C01010062, Diagenode) at RT for 10min ...
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bioRxiv - Plant Biology 2021Quote: ... Samples were sonicated three times in refrigerated Bioruptor Plus (Diagenode), 30 cycles 30sec ON/30sec OFF in a Diagenode TPX microtube M-50001 ...
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bioRxiv - Molecular Biology 2020Quote: ... Samples were sonicated three times in refrigerated BIORUPTOR Plus (Diagenode), 10 cycles 30 sec ON - 30 sec OFF in a Diagenode TPX microtube M-50001 ...
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bioRxiv - Cell Biology 2021Quote: ... sonicated three times at maximum intensity (Bioruptor®, Diagenode, Belgium) and the samples were denaturated at 65 °C for 15 min ...
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bioRxiv - Genomics 2019Quote: ... cells were sonicated 4 times 10 min in a Bioruptor (Diagenode). After sedimentation ...
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bioRxiv - Molecular Biology 2021Quote: ... 30 times for 30 s (maximum power, Bioruptor®plus, Diagenode). The lysate was clarified by ultracentrifugation (41 000 xG ...
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bioRxiv - Genomics 2023Quote: ... Samples were then vortexed (5 times) prior to sonication (Bioruptor, Diagenode), high setting ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were fixed for 10 minutes at RT in 11% formaldehyde solution in fixation buffer (Diagenode), diluted 1:10 in RPMI with 10% FBS ...
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bioRxiv - Neuroscience 2020Quote: ... and sonicated 10 times 30s on/off in a Bioruptor sonicator (Diagenode). Lysates were diluted 10-fold and quantified by BCA assay against a BSA standard curve in diluted lysis buffer ...
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bioRxiv - Plant Biology 2023Quote: ... and sonicated four times (BioruptorTM, Diagenode, 30s on/1min off on High). They were quantified at 205 nm using the Nanodrop 2000 according to the manufacturer’s instruction (Thermoscientific ...
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bioRxiv - Genomics 2020Quote: ... total on-time 10 min) with a Biorupter® Pico Sonicator Device (Diagenode). 5 - 33 μg of sonicated chromatin was incubated with 4 μg ChIP-validated CTCF antibody (Active Motif ...
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bioRxiv - Neuroscience 2022Quote: ... Lysates were sonicated with a Bioruptor Pico sonicator (Diagenode, total time 8 min) and 4μg of antibodies were added to sheared chromatin (from 4 million cells for Olig2 and from 1 million cells for histone marks ...
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bioRxiv - Cancer Biology 2020Quote: ... After sonication (30”on/30” off, 10’ total time) in a Bioruptor (Diagenode) to disrupt aggregates ...
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bioRxiv - Immunology 2020Quote: ... The cells were cross-linked with 1% formaldehyde for 10 minutes at RT and quenched with 2.5 M glycine (Diagenode) for 5 minutes at RT ...
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bioRxiv - Cell Biology 2021Quote: ... we used a total processing time of 15min per sample in a Bioruptor (Diagenode) using the following settings ...
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bioRxiv - Cell Biology 2023Quote: ... beads were incubated for 30 min at RT with 5 µg of negative control antibody (C-, IgG from healthy rabbits, Diagenode). For immunoprecipitation ...
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bioRxiv - Molecular Biology 2024Quote: ... Lysates were sonicated (5 times 5 min, 30 sec on/off) with a Bioruptor (Diagenode) and pelleted (13000 rpm ...
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bioRxiv - Molecular Biology 2024Quote: ... Lysates were sonicated (5 times 5 min, 30 sec on/off) with a Bioruptor (Diagenode) and pelleted (13000 rpm ...
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bioRxiv - Cancer Biology 2019Quote: ... 1 × 107 cells / immunoprecipitation were fixed for 30 minutes (min) at room temperature (RT) using Diagenode Crosslink Gold (Diagenode, Liège, Belgium). After two washing steps with PBS (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2019Quote: ... Samples were sonicated two times for 10 minutes (30 seconds on, 30 seconds off) by Diagenode Bioruptor Pico ...
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bioRxiv - Genomics 2022Quote: ... Cell lysates were then sonicated 20 times at 4°C (Bioruptor Plus, Diagenode, 30/30 cycles) and centrifuged at 14000 rpm for 1 minute at 4°C to remove cellular debris ...
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bioRxiv - Molecular Biology 2021Quote: ... crushed with a Dounce homogenizer and sonicated ten times for 30 s (maximum power, Bioruptorplus, Diagenode). After centrifugation at 10,000g for 10 min at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... the samples were sonicated two times for 7 min each using a precooled Bioruptor® (Diagenode). The samples were centrifuged for 10 min at 13.000 rpm and 4 °C ...
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bioRxiv - Molecular Biology 2023Quote: ... the samples were sonicated two times for 7 min each using a precooled Bioruptor® (Diagenode). The samples were centrifuged for 10 min at 13,000 rpm and 4 °C ...
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bioRxiv - Cell Biology 2023Quote: ... and subjected to sonication (30 s on and 30 s off, 80 times, Bioruptor 300 [Diagenode]). The supernatant was collected by centrifugation ...
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bioRxiv - Genetics 2020Quote: ... 30 s ON/30 s OFF for 15 min × 3 times in Bioruptor Sonication System UCD200 (Diagenode) in 15 ml polystyrene conical tubes ...
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bioRxiv - Molecular Biology 2020Quote: ... resuspended nuclei were sonicated 5 times 30 sec on followed by 30 sec off using the Bioruptor (Diagenode). Insoluble proteins were removed by centrifugation for 10 min at 14,000 rpm at 4 °C.
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bioRxiv - Molecular Biology 2021Quote: ... crushed with a Dounce homogenizer and sonicated ten times for 30 s (maximum power, Bioruptor®plus, Diagenode). After centrifugation ...
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bioRxiv - Genomics 2019Quote: ... Resultant PCR products were sheared to ∼400bp using the Bioruptor Pico (Diagenode) and Nextera XT indexes added as previously described29 ...
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bioRxiv - Molecular Biology 2019Quote: ... Samples were sonicated 12 times (30 sec on, 30 sec off) at the high intensity in a Bioruptor (Diagenode) device ...
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bioRxiv - Plant Biology 2019Quote: ... sonicated in an ice bath four times for 30 seconds on high setting using a Bioruptor UCD-200 (Diagenode) with 1.5-minute breaks on ice and supplemented with Triton-X-100 to reach a final concentration of 2 % ...
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bioRxiv - Molecular Biology 2023Quote: ... After this time, samples were sonicated twice (7 cycles 30s on, 30s off) with a Bioruptor Plus sonicator (Diagenode). In between the two sonication cycles samples were incubated at 12°C with 0.8ul of Benzonase (Sigma) ...
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bioRxiv - Biochemistry 2023Quote: ... Lysates were sonicated for 5 cycles of 30 s on/off (2.5 min total sonication time) at 4°C in a Bioruptor Plus sonication system (Diagenode) and pelleted by centrifugation at 8000 RPM for 15 min at 4°C ...
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bioRxiv - Systems Biology 2023Quote: ... By ultrasonically treating the pellet three times for 30 s with intervals of 30 s with a Bioruptor (Diagenode), the proteins precipitated with acetone for 3 h at −30 °C were dispersed in 50 mM triethylammonium bicarbonate ...
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bioRxiv - Plant Biology 2020Quote: ... Nuclei were disrupted by sonication 10 times for 30 s each and 30 s of cooling in a Bioruptor (Diagenode). IPs were performed using α-GFP (Ab6556 ...
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bioRxiv - Genetics 2020Quote: ... The top 100 µl sperm suspension was sonicated at the medium level for five times with 3 seconds each (Bioruptor UCD-200; Diagenode). An aliquot of 2 µl sperm HTF suspension was mixed immediately with 50 µl of 4% PVP (Sigma ...
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bioRxiv - Biochemistry 2020Quote: ... After incubation at 95C for 5 min the sample was sonicated 3 times for 30 sec on/off with a Bioruptor (Diagenode) on high power ...
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bioRxiv - Cell Biology 2019Quote: ... 30” by 30” at mild intensity for myoblasts and five times for nascent myotubes using a Bioruptor UCD-200 (Diagenode). The samples were diluted to a final volume of 1 ml in ChIP buffer (16 mM Tris pH 8.1 ...
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bioRxiv - Cell Biology 2020Quote: ... the lysate was diluted 10 times with lysis buffer and subjected to sonication for 15 min in a refrigerated water bath (Bioruptor, Diagenode). Part of the sonicated chromatin was treated with proteinase K and RNase A and analyzed by electrophoresis in a 1.5% agarose gel to control the size of the DNA fragments (200-500 bp) ...
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bioRxiv - Plant Biology 2019Quote: ... The extracts were then distributed into 1.5 ml reaction tubes and sonicated in an ice bath four times for 30 seconds on high setting using a Bioruptor UCD-200 (Diagenode) with 1.5-minute breaks on ice ...
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bioRxiv - Cancer Biology 2021Quote: ... 8 μg of gDNA were sonicated two times 7 min (30 sec ON /30 sec OFF) and two times 14 min (30 sec ON /30 sec OFF) with a bioruptor (Diagenode). Glucosylation and biotinylation of 5hmC were performed with the Hydroxymethyl Collector kit (Active Motif 55013) ...
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bioRxiv - Developmental Biology 2023Quote: ... The samples were sonicated five times (30-s pulses with 30-s break interval) using the Bioruptor water bath sonicator (Diagenode). The chromatin extracts were then centrifuged at 9,400 g for 10 min at 4 °C ...
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bioRxiv - Zoology 2023Quote: ... The lysate was pipetted up and down several times to homogenize the bee tissues and then sonicated (Bioruptor Pico,Diagenode) for 10 cycles consisting of a 30 s pulse and 30 s rest at 4°C ...