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Citations for Diagenode :
201 - 248 of 248 citations for Very Low Density Lipoprotein Receptor VLDLR Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... and DNA fragments (200 - 500 bp) were immunoprecipitated with a mouse monoclonal anti-5hmC antibody (2.5 μg per immunoprecipitation) following the protocol from Diagenode. The DNA-antibody mixture was incubated with magnetic beads overnight at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... 4 µg of chromatin were immunoprecipitated overnight at 4°C with 8 µg of antibody against H3K9me3 (Diagenode, C15410193) previously coupled to protein A/G magnetic beads provided in the kit ...
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bioRxiv - Molecular Biology 2022Quote: ... RNA was then incubated at 4°C overnight with or without 12.5 μg of anti-5hmC antibody (C15220001, Diagenode) in freshly prepared 1X Immunoprecipitation (IP ...
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bioRxiv - Immunology 2022Quote: ... Sonicated chromatin was diluted ten times in ChIP Dilution Buffer and immunoprecipitation was done overnight at 4°C by incubating 2.5 μl (7 μg) of anti-H3K27ac antibody (C15410196; Diagenode) precoated on 25-μl protein A-coated magnetic beads (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2022Quote: ... 2 mg of chromatin lysate was rotated overnight at 4°C with 5 μg antibody: rabbit IgG (Diagenode #C15410206), anti-NIPBL (NIPBL#1 ...
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bioRxiv - Molecular Biology 2023Quote: ... and were directly subjected to immunoprecipitation with corresponding antibodies (H3K4me3: Diagenode, C15410003; H3K27me3: Diagenode, C15410195; CTCF: Millipore 07-792). Library preparation was carried out using NEBNext Ultra II DNA Library Prep kit (NEB E7645) ...
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bioRxiv - Cancer Biology 2023Quote: ... Isolated nuclei from primary fallopian tube secretory epithelial cells bound to concalavin A beads were incubated with primary antibody that detects H3K27ac (0.7μg, Diagenode, RRID:AB_2637079) at 4℃ overnight ...
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bioRxiv - Genomics 2023Quote: ... The sample was then incubated with antibodies (AR: Abcam ab133273, FOXA1: Abcam ab23738, H3K27ac: Diagenode 3C15410196; H3K4me3: Diagenode 3C15410003) coupled with Dynabeads protein A and protein G beads (Life Technologies ...
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bioRxiv - Plant Biology 2024Quote: ... Primary antibody incubation was performed overnight at 4°C with gentle shaking using anti-H3K9me2 (Diagenode Cat. No. C15410060), Anti-Histone H3 (Sigma-Aldrich Cat ...
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bioRxiv - Microbiology 2024Quote: ... Immunoprecipitation was done with 2 µg of chromatin and 4 µg of antibody against H3K9me3 (Diagenode, C15410193, lot A2217P). Sequencing libraries were prepared from 5 ng of immunoprecipitated DNA as previously described [19 ...
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FOXO dictate initiation of B cell development and myeloid restriction in common lymphoid progenitorsbioRxiv - Immunology 2022Quote: ChIPseq was performed as previously described (Bouderlique et al., 2019) using H3K27Ac antibodies (Diagenode Cat #C15410196, lot A1723-0041D/2) and the ThruPLEX DNA-seq (Rubicon Genomics ...
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bioRxiv - Plant Biology 2019Quote: ... For 5-mC immunolocalization slides were first incubated over night at 4°C with a monoclonal anti-5-mC-antibody (Diagenode C15200003 ...
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bioRxiv - Molecular Biology 2019Quote: ... or 2 ng (other antibodies) of ChIP-ed DNA was used to prepare DNA libraries with MicroPlex Library Preparation Kit v2 (Diagenode).
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bioRxiv - Microbiology 2022Quote: ... Immunoprecipitation was done on chromatin corresponding to the remaining 10 000 cells using antibodies against H3K4me3 (Diagenode, cat no. C15410003) – 10% of the chromatin was used for input ...
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bioRxiv - Microbiology 2023Quote: ... chromatin-antibody complexes were washed and eluted from the beads using the iDeal ChIP-seq kit for transcription factors (Diagenode) according to the manufacturer’s protocol ...
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bioRxiv - Genetics 2023Quote: ... We assessed two histone marks H3K4me3 and H3K27me3 and the ChIP grade antibodies against for these marks procured from Diagenode (H3K4me3 ...
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bioRxiv - Bioengineering 2023Quote: ... Membranes were blocked using 5% BSA in 1X TBST and incubated overnight with primary antibody (anti-Cas9; 1:1000 dilution, Diagenode #C15200216 ...
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bioRxiv - Cell Biology 2023Quote: ... which were incubated overnight on a rotatory wheel at 4°C with the primary antibodies H4K20me1 (Diagenode; #C15410034; 2 μg), H3K9me3 (Diagenode ...
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bioRxiv - Cell Biology 2023Quote: ... Immunostaining was performed overnight at +4°C with primary antibodies (H3K4me2 1:1000; Abcam ab32356) (H3K4me3 1:200; Diagenode C15410003) (H3K4me2 1:1000 ...
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bioRxiv - Genomics 2023Quote: ... and the appropriate amount of antibody (1ug of α-H3K27ac and 0.5ug of α-H3K4me1; Diagenode #C15410196 and #C15410194 respectively). Samples were incubated rotating at 4°C overnight ...
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bioRxiv - Cell Biology 2023Quote: ... beads were incubated for 30 min at RT with 5 µg of negative control antibody (C-, IgG from healthy rabbits, Diagenode). For immunoprecipitation ...
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bioRxiv - Genomics 2023Quote: ... Preclearing 30μl of Dynabeads protein A/G for 1 h at 4 °C was followed by incubation with antibodies (H3K27ac, Diagenode, 3C15410196 ...
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bioRxiv - Biophysics 2024Quote: ... Click or tap here to enter text.Both strained culture plates and unstrained control plates were incubated with primary antibodies to detect H3K9me3 (Diagenode, C15200146) and HP1a (Abcam ...
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bioRxiv - Evolutionary Biology 2020Quote: ... We reserved 10% of the pooled fragmented sample for the input (fragment control) and used the remaining 90% to perform the chromatin pull‐down (ChIP sample, target antibody: H3K9me3 polyclonal classic Diagenode C15410056) according to the 100,000 cell specifications ...
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bioRxiv - Bioengineering 2020Quote: ... for 1 h at room temperature. The anti-AAV2 (A20, cat. # 03-65155, American Research Products, Inc) and anti-SpCas9 antibody (Diagenode C15310258) was used as positive control for detection of anti-AAV9 and anti-SpCas9 antibodies ...
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bioRxiv - Evolutionary Biology 2021Quote: ... We reserved 10% of the pooled fragmented sample for the input (fragment control) and used the remaining 90% to perform the chromatin pull-down (ChIP sample, target antibody: H3K9me3 polyclonal classic Diagenode C15410056) according to the 100,000 cell specifications ...
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bioRxiv - Molecular Biology 2021Quote: ... A portion of sheared DNA (10%) was kept as input and the rest of the sheared DNA was immunoprecipitated with α-5-methylcytosine antibody (Diagenode), bound to magnetic beads ...
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bioRxiv - Genomics 2022Quote: ... and 800ul of antibody buffer containing 1ul of primary antibodies (normal rabbit IgG, Santa Cruz Cat no sc-2027, H3K27me3 (Millipore, Catalogue number 07-449, Lot DAM1703508 and H3K9me3 (Diagenode, Catalogue number C15410193 ...
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bioRxiv - Cancer Biology 2020Quote: ... samples were incubated by rotating overnight at 4°C with primary antibody or irrelevant IgGs and then incubated with protein-A agarose beads (Diagenode; C03020002) previously blocked with 0.5% BSA for 3 hr at 4 °C with rotation ...
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bioRxiv - Plant Biology 2020Quote: ... the chromatin was sheared to an average length of 500 bp by sonication and then immunoprecipitated with the H3K9ac antibody (Diagenode, C15410004). The cross-linking was then reversed ...
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bioRxiv - Developmental Biology 2019Quote: ... 2.5 μ 10% Triton X-100 and 1 l of anti-methylcytidine antibody (1 mg/ml Diagenode # MAb-081-μ 100) overnight ...
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bioRxiv - Microbiology 2021Quote: ... The presence of various histone methylation marks with PfCD bound nucleosome complexes were analyzed using modification specific antibodies against H3K9me3 (Diagenode # C15410193), H3K36me3 (Diagenode # C15410192) ...
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bioRxiv - Developmental Biology 2021Quote: ... 10% goat serum in PBST was used to block embryos before addition of pre-blocked Pol II S2P monoclonal antibody (Diagenode, C15200005) at 1:1000 dilution ...
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bioRxiv - Cell Biology 2023Quote: ... Each chromatin sample containing about 6,000 cells was incubated with 1 µg of anti-H3K27me3 antibody (Diagenode, C15410195, lot. A1811-001P). ChIP was performed using the automated platform SX-8G IP-Star platform (Diagenode ...
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bioRxiv - Genomics 2023Quote: ... Approximately 10 μg chromatin was aliquoted and diluted to 500 μL of 1x ChIP Buffer and immunoprecipitated using 1.4 μg anti-H3K4me3 antibody (pAb-003-050, Diagenode, Denville, NJ). In some cases ...
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bioRxiv - Neuroscience 2023Quote: ... RNA samples were incubated overnight at 4°C with 12.5 µg of anti-5-hmC antibody (Diagenode rat monoclonal MAb-633HMC) or without antibody as negative control in IP buffer (750 mM NaCl ...
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bioRxiv - Genomics 2023Quote: ... A portion of sheared DNA (10 %) was kept as input and the rest of the sheared DNA was immunoprecipitated with α-5-methylcytosine antibody (Diagenode), bound to magnetic beads ...
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bioRxiv - Genomics 2023Quote: ... Preclearing 30μl of Dynabeads protein A/G for 1 h at 4 °C was followed by incubation with antibodies (H3K27ac, Diagenode, 3C15410196; H3K4me3, Diagenode 3C15410003). Reverse-crossed IP sample were pulled down with streptavidin C1 beads (Life Technologies) ...
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bioRxiv - Molecular Biology 2024Quote: ... IP was performed overnight at 4° C using 5 μg antibody (anti-H3K9me3 Diagenode, ref. C15410056; anti-HA.11 BioLegend ref: 901503; anti-H3K4me3 Cell Signaling, ref. 9751S; anti-H3K36me3 Diagenode, ref ...
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bioRxiv - Cell Biology 2020Quote: ... 600 ng of denatured DNA was incubated with 2 mg of 5-methylcytosine (5-mC) monoclonal antibody ([33D3] Diagenode, Cat No # C15200081) in IP buffer (0.1% SDS ...
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bioRxiv - Developmental Biology 2022Quote: ... were then incubated overnight at 4°C using primary antibodies for H3K27ac and SET in iC buffer from iDeal ChIP-seq kit for Histones (Diagenode, cod. C01010051). The next day ...
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bioRxiv - Cancer Biology 2023Quote: ... Chromatin corresponding to 1 μg was immunoprecipitated using the following antibodies and amounts: 1 μg of H3K27ac (Diagenode C15410196; Lot A1723-00410). Chromatin corresponding to 1% was set apart as Input ...
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bioRxiv - Molecular Biology 2021Quote: ... The membranes were incubated with blocking buffer (5% milk powder in TBST) for one hour at room temperature and afterwards incubated with primary antibody (anti-H3K27me3, Diagenode # C15410195, 1:1000) in blocking buffer overnight at 4 °C ...
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bioRxiv - Neuroscience 2021Quote: ... equal volume of sheared chromatin was incubated overnight with 3 μg of antibody in iC1b buffer with protease inhibitors and BSA and washed DiaMag Protein A-coated magnetic beads (Diagenode Cat# C03010020-220) on a tube rotator at 4°C overnight ...
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bioRxiv - Genetics 2021Quote: ChlPseq was performed on 20k FACS sorted MM cells as previously described 44 using H3K27Ac antibodies (Diagenode Cat #C15410196, lot A1723-0041D/2) but with minor modifications on the preparation of input controls ...
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bioRxiv - Developmental Biology 2020Quote: ... All ChIPs were incubated overnight with the respective antibodies (CTCF Active Motif 61311; H3K27me3 Millipore 17-662; H3K27ac Diagenode C15410196; RAD21 abcam ab992) and precipitated after a 2h long incubation with magnetic beads (Dynabeads Protein A ...
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bioRxiv - Cancer Biology 2023Quote: ... The resulting chromatin was incubated overnight at 4°C with histone modification antibodies (H3K27ac, Abcam ab4729; H3K27me3, Diagenode C15410195 and H3, Abcam ab1791) pre-conjugated with Dynabeads Protein G ...
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bioRxiv - Genomics 2023Quote: ... The resulting chromatin was incubated overnight with the following antibodies (H3K27ac, Diagenode, Catalog No: C15410196 LOT: A1723-0041D; H3K4me2, Diagenode, Catalog No: C15410035 LOT: A936-0023) coupled with 40 μl protein A and protein G beads (Invitrogen ...