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Citations for Diagenode :
301 - 350 of 424 citations for Transcription Factor SOX 10 SOX10 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2021Quote: ... we obtained 5 μg of high molecular weight genomic DNA (> 50% of fragments ≥ 40 kb) and sheared fragments to ~10 kb using the Megaruptor 3 (B06010003; Diagenode), followed by DNA repair and ligation of PacBio adapters using the SMRTbell Template Prep Kit v1.0 (100-991-900) ...
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bioRxiv - Plant Biology 2020Quote: ... samples were placed on ice for 10 min and sonicated for 6 cycles 15 sec “on” – 15 sec “off” using Bioruptor Plus (Diagenode). After that ...
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bioRxiv - Cell Biology 2021Quote: ... 10 mM TCEP was added and the pellet was sonicated in a Bioruptor Pico cooled by Minichiller 300 (both Diagenode). Chloroacetamide was added to a final concentration of 15 mM and samples were incubated for 10 min at 95°C with gentle agitation ...
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bioRxiv - Developmental Biology 2022Quote: ... in VFG media for 10 minutes at room temperature followed by a 5-minute quench with glycine (in True MicroChIP kit, Diagenode) at room temperature ...
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bioRxiv - Biochemistry 2021Quote: ... The lysates were sonicated (3 × 10 s bursts with 5 s rest) on ice at low amplitude in a Bioruptor (Diagenode) and then centrifuged at 20,817 × g for 30 min at 4°C ...
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bioRxiv - Developmental Biology 2020Quote: ... Tubes were sonicated for 10 mins (sonication cycle: 30 sec ON, 30 sec OFF) at 4 °C in a Bioruptor ultrasonication bath (Diagenode) at high energy setting ...
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bioRxiv - Genomics 2022Quote: ... 20 mM Na Butyrate) and sonicated for 30 sec ON/OFF for 10 min in a Bioruptor®Pico (Diagenode) to generate 200-500 bp DNA fragments ...
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bioRxiv - Cancer Biology 2022Quote: ... 10-15 cycles of sonication (30 sec ON, 30 sec OFF) were carried using the Bioruptor Pico sonicator device (Diagenode). H3k27ac antibody was obtained from Abcam (ab4729 ...
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bioRxiv - Neuroscience 2019Quote: ... followed by vortexing and sonication (20 cycles of 15 seconds ON/OFF, 10 minutes in total; Bioruptor plus (model UCD-300, Diagenode)) ...
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bioRxiv - Cell Biology 2019Quote: ... in the presence of protease inhibitors and subjected to 3 × 10 cycles (30 seconds on and 30 seconds off) of sonication (Bioruptor, Diagenode) to generate chromatin fragments of ∼300 bp in length ...
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bioRxiv - Developmental Biology 2019Quote: ... resuspended in 100 μl of ChIP buffer 1 and kept on ice for 10 minutes, followed by sonication (3x 30 seconds on/off, high output) using Bioruptor sonicator (Diagenode). The lysate was cleared by centrifugation at 10,000 rpm ...
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bioRxiv - Cell Biology 2020Quote: ... the lysate was diluted 10 times with lysis buffer and subjected to sonication for 15 min in a refrigerated water bath (Bioruptor, Diagenode). Part of the sonicated chromatin was treated with proteinase K and RNase A and analyzed by electrophoresis in a 1.5% agarose gel to control the size of the DNA fragments (200-500 bp) ...
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bioRxiv - Cell Biology 2020Quote: ... Lysates were incubated on ice for 10 minutes followed by sonication 5x for 30 sec on/off on high using a Bioruptor (Diagenode). Lysates were then spun at 10,000 x g for 10 minutes at 4°C ...
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bioRxiv - Genetics 2020Quote: S2 cells transfected with either mCherry-Nnk or Odj-mCherry for 72 hours were fixed with 1% paraformaldehyde for 10 minutes and sheared with Bioruptor sonicator (Diagenode) to obtain chromatin ...
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bioRxiv - Neuroscience 2020Quote: PFF transduction was conducted at 10 DIV by adding 2 ug/ml (96 well plate and coverslips) or 10 ug/ml (biochemistry) of PFFs diluted in PBS and sonicated with a BioRuptor (Diagenode) bath sonicator for 10 cycles of 30 seconds on ...
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bioRxiv - Microbiology 2019Quote: ... washed cell pellets were lysed in 50 mM HEPES pH 8 with 5% SDS followed by 10 min (30 sec on/off) sonication in a Bioruptor Pico sonicator (Diagenode) at 18 °C ...
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bioRxiv - Molecular Biology 2021Quote: ... cells were cross-linked for 10 min at room temperature with 1% formaldehyde before lysis followed by sonication of their chromatin (Bioruptor Plus, Diagenode) to obtain DNA fragments of 200–400 bp ...
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bioRxiv - Biochemistry 2021Quote: ... The lysates were sonicated (3 × 10 s bursts with 5 s rest) on ice at low amplitude in a Bioruptor (Diagenode) and then centrifuged at 20,817 × g for 30 min at 4°C ...
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bioRxiv - Cancer Biology 2021Quote: ... To improve the proportion of DNA fragments at the optimal length for library preparation samples were sonicated for 10 cycles using the Bioruptor Pico Sonication Device (Diagenode). FFPE DNA samples were treated with the NEBNext FFPE DNA Repair Mix (NEB ...
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bioRxiv - Genomics 2020Quote: ... nuclei were isolated via sonication using a Bioruptor Pico (Diagenode; 9 cycles of 10 sec on and 30 sec off). Chromatin was then sheared in the recommended shearing buffer (27-30 cycles ...
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bioRxiv - Cell Biology 2022Quote: ... cells were sonicated for 10 cycles of 30 sec “on” and 30 sec “off” at 4°C with Bioruptor (Diagenode) and the protein concentration was measured using Bradford protein assay (Thermo Fisher Scientific) ...
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bioRxiv - Biophysics 2022Quote: ... samples were sonicated for 15 min (30 sec on, 30 sec off at 10°C) with a Bioruptor Pico (Diagenode). Then ...
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bioRxiv - Molecular Biology 2023Quote: ... and sonicated for 10 min (medium intensity, 30 s on/30 s off) in a Bioruptor UCD-200 system (Diagenode), and centrifuged at 21,000 x g for 5 min ...
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bioRxiv - Plant Biology 2023Quote: ... Nuclei were lysed in NLB (50 mM Tris-HCl pH8, 10 mM EDTA, 1% SDS and 1x Proteases Inhibitor cocktail. Chromatin was sonicated using a Diagenode Bioruptor (40 Hz ...
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bioRxiv - Immunology 2022Quote: ... with a freshly added protease inhibitor cocktail and sodium vanadate and subjected to 9 cycles (30s ON-10s OFF) of ultrasonication (Bioruptor Plus; Diagenode). The sonicated samples are then spun down ...
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bioRxiv - Neuroscience 2022Quote: ... A modified protocol for on-beads digest (sonication for 10*30s in a Bioruptor sonicator in presence of 50 mg Protein Extraction beads, C20000021, Diagenode) was used for tissue samples followed by a 3.5h digestion step ...
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bioRxiv - Molecular Biology 2022Quote: ... Protease inhibitor and RNase inhibitor) and sonicated for 10 cycles (30 secs on 30 secs off) on a Bioruptor (Diagenode) sonicator ...
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bioRxiv - Genetics 2023Quote: ... third instar larval wing discs were prepared by boiling samples for 10 minutes in Laemmli SDS-PAGE loading buffer followed by sonication using the Bioruptor Pico sonication system (Diagenode) for 10 cycles (30 sec on ...
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bioRxiv - Genomics 2023Quote: ... Lysates were sonicated for 10-15 cycles of 30s ON/30s OFF on high power using the Bioruptor Plus (Diagenode), then diluted in additional lysis buffer 3 and clarified by centrifugation for 10 min at max speed at 4°C ...
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bioRxiv - Immunology 2023Quote: ... Cells were then lysed to isolate chromatin and sonicated for 10-20 min (depending on cell type) using a Bioruptor (Diagenode). Fragmentation of sonicated chromatin to lengths of 200-500 bp was confirmed by agarose gel electrophoresis.
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bioRxiv - Developmental Biology 2023Quote: ... Samples (∼200 µL) were then subjected to sonication (30 sec on/off cycles, 10 cycles) using a Bioruptor Pico sonication instrument (Diagenode). Whole cell lysates were cleared by centrifugation and protein concentrations were determined using the DC Protein Assay (BioRad ...
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bioRxiv - Biochemistry 2023Quote: ... Cell lysis was obtained by pulsed ultrasonication (10 cycles, 30” On, 30” Off, at High intensity using Diagenode Bioruptor 300), complete lysis was monitored by phase contrast microscopy ...
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bioRxiv - Immunology 2023Quote: ... Nuclei preparation and shearing were performed as described previously53 with 10 cycles sonication (30s ON/30s OFF, Bioruptor Pico, Diagenode). PU.1 was immunoprecipitated overnight at 4°C using a polyclonal anti-PU.1 antibody (1:25 ...
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bioRxiv - Molecular Biology 2023Quote: Libraries were then prepared from 10 ng of RPFs using the D-Plex Small RNA-Seq kit for Illumina (Diagenode) and the D-Plex Unique Dual Indexes for Illumina – set A (Diagenode) ...
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bioRxiv - Immunology 2023Quote: ... or ChIP proceeded by sonicating with for 40 min (30 sec on/off 10 min cycles) in a Bioruptor UCD-200 (Diagenode). Lysates were cleared and 10% taken for input samples.
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bioRxiv - Neuroscience 2023Quote: ... At the last step of the protocol both nuclear and cytoplasmic samples were sonicated for 10 cycles (30 seconds on / 30 seconds off) using the Bioruptor Pico (Diagenode). For the preparation of the nuclear extraction and fractionation buffer the reagents used were HEPES (Sigma-Aldrich ...
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bioRxiv - Genomics 2023Quote: ... 1x protease inhibitor) and sonicated for 10 cycles (sonication cycle: 30 sec ON, 30 sec OFF) using Bioruptor Plus (Diagenode). The samples were centrifugated at 14000x g for 10 minutes at 4°C and the supernatant containing the soluble chromatin was transferred to a fresh low-binding tube ...
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bioRxiv - Systems Biology 2024Quote: ... Afterwards the lysate was sonicated for 15 cycles of 30 sec ON/OFF at 10 °C using a Bioruptor (Diagenode) and subsequently frozen at -80 °C ...
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bioRxiv - Cell Biology 2024Quote: ... Cell lysates were sonicated at 4°C for 10 min (30 sec ON, 30 sec OFF) at medium frequency (Bioruptor Diagenode). Then ...
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bioRxiv - Cancer Biology 2021Quote: The HiChIP protocol was performed as previously described (Mumbach 2016) using 7.5µg antibody to H3K27ac (Diagenode, C15410196) with the following modifications ...
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bioRxiv - Developmental Biology 2021Quote: ... 3µm-sections were immunostained using an antibody anti-histone H3 containing the trimethylated lysine 27 (H3K27me3) (Diagenode). Heat induced antigen retrieval was done using CC1 basic buffer (Ventana) ...
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bioRxiv - Genetics 2020Quote: ... Immunoprecipitation was performed using 75 µg of chromatin and 5 µg of Cas9 antibody (Diagenode #C15200229-100) or 15 µg of chromatin and 2.5 µg of H3K27ac and H3K9me3 antibody (Active Motif #3913 ...
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bioRxiv - Genetics 2020Quote: ... that had been pre-blocked with 0.5% BSA and mixed with polyclonal CTCF antibody (C15410210-50, Diagenode) or polyclonal H3K9me3 antibody (AB_2532132 ...
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bioRxiv - Cancer Biology 2021Quote: ... The DNA-protein-antibody complexes were captured by DiaMag protein A-coated magnetic beads (Diagenode; Cat#: C03010020) by incubating at 4 °C for 2 hours ...
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bioRxiv - Cancer Biology 2021Quote: ... The DNA-protein-antibody complexes were captured by DiaMag protein A-coated magnetic beads (Diagenode; Cat#: C03010020) by incubating at 4 °C for 2 hours ...
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bioRxiv - Genomics 2020Quote: ... The latter aliquot was processed for ChIP using 3 μg anti-H3K4me3 antibody (pAb-003-050, Diagenode) as indicated in the user manual ...
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bioRxiv - Microbiology 2023Quote: ... the different antibodies were previously incubated at 4°C with protein G-coated magnetic beads (DiaMag, Diagenode), protease inhibitor cocktail and 0.1% BSA ...
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bioRxiv - Genomics 2023Quote: ... about half of the chromatin extract was used for immunoprecipitation with 5µg of H3K27me3 antibody (C15410069 Diagenode). IP ...
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bioRxiv - Microbiology 2020Quote: ... was added to the lysate and incubated at 37 °C for 10 min before sonication (2 × 8 bursts of 30 sec on ice using a Diagenode Bioruptor) to shear DNA fragments to a length of 300 to 500 bp ...
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bioRxiv - Microbiology 2022Quote: ... were added to the lysate and incubated at 37 °C for 10 min before sonication (2 x 8 bursts of 30 sec on ice using a Diagenode Bioruptor) to shear DNA fragments to a length of 300 to 500 bp ...