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Citations for Diagenode :
101 - 150 of 226 citations for Tetanus Toxoid Recombinant Heavy Chain Fragment C since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... 50 mM NaCl) and sonicated for 30 minutes at 4°C (Diagenode Bioruptor). Anti-di-glycine peptide beads (Cell Signalling #5562 ...
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bioRxiv - Cell Biology 2022Quote: ... Chromatin was incubated overnight at 4°C using 0.25µg of H3K9me3 (C15410193-Diagenode) per IP ...
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bioRxiv - Plant Biology 2021Quote: ... Sonication of chromatin was done in 4°C water sonicator (Diagenode Bioruptor Plus). Sonicated samples were first precleared with Protein A Agarose beads (Millipore #16-157 ...
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bioRxiv - Synthetic Biology 2021Quote: ... with following antibodies at 4 °C on a rotating wheel: H3K27me3 (Diagenode, C15410195), Ring1B (Cell Signaling ...
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bioRxiv - Microbiology 2023Quote: ... 570 µM PMSF) for 10 min at 4 °C with sonication (Bioruptor, Diagenode, 10 min on ice ...
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bioRxiv - Plant Biology 2024Quote: ... slides were incubated over night at 4°C with primary-antibody (H3K9me2: Diagenode: C15200154 ...
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bioRxiv - Plant Biology 2020Quote: ... The resuspended chromatin was sonicated in a 4°C water sonicator (Diagenode Bioruptor Plus). Serum containing anti-E2Fa antibodies was obtained from Prof ...
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bioRxiv - Neuroscience 2021Quote: ... sonicated for 3×5 minutes in a 4°C Bioruptor ultrasonicator (UCD-200, Diagenode), vortexed ...
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bioRxiv - Biochemistry 2020Quote: ... samples were sonicated for 15 minutes (37°C) using a Bioruptor plus ultrasonicator (Diagenode). The second digestion step was done using fresh LysC (1:50 enzyme to protein ratio ...
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bioRxiv - Synthetic Biology 2023Quote: ... heated at 95°C for 10 min and sonicated with the Bioruptor sonicator (Diagenode) for 15 cycles (30 s ON ...
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bioRxiv - Genomics 2023Quote: ... in the high mode at 4°C in a Bioruptor water bath sonicator (Diagenode), to obtain fragments of 500 to 1000 bp in size ...
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bioRxiv - Molecular Biology 2024Quote: ... IP was performed overnight at 4° C using 5 μg antibody (anti-H3K9me3 Diagenode, ref ...
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bioRxiv - Microbiology 2024Quote: ... before and after sonication at 4°C for 15min in a Bioruptor® (Diagenode), to determine Chain (ChFU ...
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bioRxiv - Genomics 2020Quote: ... Purified DNA was sonicated to obtain fragments of an average size of 300-400bp using a Bioruptor Pico (Diagenode; 8 cycles; 20s on and 60s off). 3μg of sonicated DNA was used for library preparation ...
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bioRxiv - Evolutionary Biology 2024Quote: ... DNA concentration was normalized (except for low quality samples) and mechanically fragmented to 300-600 bp fragments using a Bioruptor Pico (Diagenode; 15s ON, 90s OFF, eight cycles). Low quality DNA were then reconcentred with AMPure XP beads and libraries were constructed according to the NEBNext® Ultra II DNA Library Prep for Illumina® kit recommendations (New England Biolabs ...
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bioRxiv - Molecular Biology 2020Quote: ... Lysates were denatured at 95°C and DNA was sonicated using a Bioruptor 300 (Diagenode) on the high setting for 8 cycles 30 sec ON / 30 sec OFF ...
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bioRxiv - Molecular Biology 2021Quote: ... with following appropriate antibodies at 4 °C o/n a rotating wheel: H3K27me3 (Diagenode, C15410195), RING1B (Cell Signaling ...
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bioRxiv - Cancer Biology 2023Quote: ... 30 s on / 30 s off) at 4°C using a water bath-sonicator (Diagenode). Chromatin fragments were reverse cross-linked overnight at 65°C ...
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bioRxiv - Genomics 2023Quote: ... 6μL DNA extract were incubated (55°C) with 5μL of 2X Tagmentation Buffer (Diagenode, C01019043) and 1μL of Loaded Tn5 Tagmentase (Diagenode ...
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bioRxiv - Cancer Biology 2020Quote: ... Purified DNA was fragmented to obtain fragments of an average size of 300–400 bp using a Bioruptor Pico (Diagenode; 8 cycles; 20 sec ON/60 sec OFF). 3 μg of DNA per condition were used for library preparation ...
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bioRxiv - Molecular Biology 2021Quote: ... shear DNA and enhance cell disruption by using a water-bath (4°C) sonicator Bioruptor (Diagenode) at High Power for 40 min (sonication cycle ...
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bioRxiv - Genomics 2021Quote: ... After sonication (15 sec on/ 30 sec off, 5 cycles, 4 C) using Bioruptor 300 (Diagenode) 5 µl were stained with DAPI to verify isolation of intact Macs ...
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bioRxiv - Biochemistry 2022Quote: ... Samples were heated at 72 °C for 5 min and sonicated using a Bioruptor (Diagenode Inc) with fifteen 30 s on/off cycles at maximum intensity ...
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bioRxiv - Genomics 2022Quote: ... Cell lysates were then sonicated 20 times at 4°C (Bioruptor Plus, Diagenode, 30/30 cycles) and centrifuged at 14000 rpm for 1 minute at 4°C to remove cellular debris ...
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Rbx1 Cullin-Ring ligase E3 and Ube2m neddylation E2 differentially governs the fitness of Treg cellsbioRxiv - Immunology 2020Quote: ... and subsequently lysed by 5min boiling at 95°C and sonication (Bioruptor, model UCD-200, Diagenode) for 15min ...
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bioRxiv - Molecular Biology 2022Quote: ... After sonication for 5 cycles of 30 seconds ON/OFF at 4 °C (Bioruptor Pico, Diagenode), the mixture was centrifuged (13 000xg ...
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bioRxiv - Cancer Biology 2023Quote: ... The isolated chromatin was fragmented by sonication at 4°C for 10 minutes using Bioruptor (Diagenode). Run-on reactions were performed at 37°C for 5 minutes using biotinylated nucleotides (5 mM Tris-HCl pH 8.0 ...
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bioRxiv - Physiology 2023Quote: ... 500µl volume) in a water bath sonicator set at 4°C (Bioruptor 300; Diagenode, Denville, NJ). After centrifuging at 10000g for 10 minutes at 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... and subsequently disrupted by 2 × 20” high intensity sonication cycles at 4°C in a BioRuptor (Diagenode). Protein concentration was determined using the Micro BCA™ protein assay kit according to manufacturer’s instructions (Thermo Scientific) ...
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bioRxiv - Biochemistry 2022Quote: ... Cells were disrupted by sonication (two cycles à 20 s at 4 °C) in a BioRuptor (Diagenode), and extracts were cleared by centrifugation at 15,000 x g for 1 min ...
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bioRxiv - Genetics 2021Quote: ... incubated on ice for 10 min and subsequently sonicated at 4 °C with a Bioruptor sonicator (Diagenode) at high power for 5 cycles with 30 s ON and 30 s OFF ...
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bioRxiv - Genomics 2019Quote: Immunoprecipitations from 105 cells were performed using IP star robot at 4°C (indirect 200µl method, Diagenode) with an anti-BrdU antibody (10μg ...
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bioRxiv - Genomics 2022Quote: ... the nuclei were briefly sonicated for 5 seconds at 4°C in a Bioruptor Pico sonicator (Diagenode). Permeabilized nuclei were incubated for 10 minutes at 37°C in a thermoshaker ...
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bioRxiv - Microbiology 2023Quote: ... the different antibodies were previously incubated at 4°C with protein G-coated magnetic beads (DiaMag, Diagenode), protease inhibitor cocktail and 0.1% BSA ...
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bioRxiv - Microbiology 2023Quote: ... The mixture was then placed in a thermostated (50°C) ultrasonic bath (Bioruptor Plus, Diagenode, Liège, Belgium); ultrasounds were applied at low power for 10 cycles (30 seconds on – 30 seconds off) ...
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bioRxiv - Developmental Biology 2021Quote: ... snap frozen and stored at −80 °C or further processed using iDeal ChIP for Transcriptional Factors Kit (Diagenode) (Cat ...
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bioRxiv - Molecular Biology 2021Quote: ... Cross-linking was reversed at 65°C overnight and immunoprecipitated DNA was recovered using the IPure Kit (Diagenode). Genomic regions of interest were identified by realtime PCR (qPCR ...
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bioRxiv - Genetics 2023Quote: ... The mixture was heated to 95°C for 10 min and sonicated in a Bioruptor (Diagenode, Liege, Belgium) (30 sec sonication ...
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bioRxiv - Neuroscience 2023Quote: ... The protein was then incubated at 37 °C for 120 hours with constant agitation at 200 rpm and the product sonicated 10 min at 10 °C (0.5 min on/0.5 min off) using a Biorupter Pico (Diagenode). Aggregates were flash-frozen with liquid nitrogen in single-use aliquots for storage at -80 °C.
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bioRxiv - Physiology 2023Quote: ... and 500-µl volume) in a water bath sonicator set at 4°C (Bioruptor 300. Diagenode, Denville, NJ). After centrifuging at 10,000g for 10 mins at 4°C ...
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bioRxiv - Plant Biology 2024Quote: ... slides were incubated over night at 4°C with primary-antibody (H3K9me2: Diagenode: C15200154; H3K27me1: Diagenode: C15410045-50) diluted in 1% BSA ...
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bioRxiv - Developmental Biology 2024Quote: ... The sonication of samples was then performed at 4°C using a Bioruptor® Plus sonication device (Diagenode) at high-intensity settings ...
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bioRxiv - Immunology 2021Quote: ... sonicated at 4°C with 3 cycles of 30 s on 30 s off in a Bioruptor Pico (Diagenode) and clarified by centrifugation at 12,000 × g ...
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Multi-Omic Analysis Reveals Disruption of Cholesterol Homeostasis by Cannabidiol in Human Cell LinesbioRxiv - Systems Biology 2021Quote: ... SDS lysates were boiled at 95°C for 10 minutes and then 10 cycles in a Bioruptor Pico (Diagenode) of 30 seconds on and 30 second off per cycle ...
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Deep learning the collisional cross sections of the peptide universe from a million training samplesbioRxiv - Systems Biology 2020Quote: ... The samples were boiled at 95°C for 10 min and subsequently sonicated at maximum power (Bioruptor, Diagenode, Belgium). Proteolytic digestion was performed overnight at 37 °C by adding either i ...
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bioRxiv - Immunology 2020Quote: ... sonicated at 4°C with 3 cycles of 30 s on 30 s off in a Bioruptor Pico (Diagenode) and clarified by centrifugation at 12,000 xg ...
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bioRxiv - Microbiology 2021Quote: ... 4 µg of chromatin were immunoprecipitated overnight at 4°C with 8 µg of antibody against H3K9me3 (Diagenode, C15410193) previously coupled to protein A/G magnetic beads provided in the kit ...
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bioRxiv - Molecular Biology 2022Quote: ... RNA was then incubated at 4°C overnight with or without 12.5 μg of anti-5hmC antibody (C15220001, Diagenode) in freshly prepared 1X Immunoprecipitation (IP ...
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bioRxiv - Immunology 2022Quote: ... Sonicated chromatin was diluted ten times in ChIP Dilution Buffer and immunoprecipitation was done overnight at 4°C by incubating 2.5 μl (7 μg) of anti-H3K27ac antibody (C15410196; Diagenode) precoated on 25-μl protein A-coated magnetic beads (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2022Quote: ... 2 mg of chromatin lysate was rotated overnight at 4°C with 5 μg antibody: rabbit IgG (Diagenode #C15410206), anti-NIPBL (NIPBL#1 ...