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Citations for Diagenode :
201 - 250 of 279 citations for SARS CoV 2 Spike Glycoprotein S1 His tag Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: ... Cells were sonicated in 6 x 30 second intervals using a Bioruptor 300 Machine (Diagenode), then centrifuged for 10 minutes at 10,000g at 4°C ...
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bioRxiv - Genomics 2022Quote: ... Cells were lysed and DNA-protein complexes were sheared using the Bioruptor Pico (Diagenode, #B01060010) for 8 minutes ...
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bioRxiv - Genetics 2024Quote: ... Cell lysates were transferred to Diagenode 15 ml sonication tubes and sonicated (Bioruptor pico, Diagenode) 30 sec ON/ 45 sec OFF at 4°C for 60 cycles (6x10) ...
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bioRxiv - Cell Biology 2023Quote: ... Cell lysates were sonicated for 15 min at high power with the Bioruptor sonicator (Diagenode). Protein quantification was performed using the Bradford reagent (Biorad #5000001) ...
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bioRxiv - Molecular Biology 2021Quote: ... shear DNA and enhance cell disruption by using a water-bath (4°C) sonicator Bioruptor (Diagenode) at High Power for 40 min (sonication cycle ...
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bioRxiv - Biochemistry 2019Quote: ... Whole-cell lysates were prepared for immunoblotting by sonicating with a Bioruptor (Diagenode, Inc., Denville, NJ) for 3 min and boiling for 15 min.
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bioRxiv - Genetics 2019Quote: ... The cells were then sonicated in buffer iS1 using the Bioruptor® Pico sonication device (Diagenode) to achieve fragment sizes distribution below 3 kb.
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bioRxiv - Cancer Biology 2020Quote: ... cells were fixed for 10 minutes at RT in 11% formaldehyde solution in fixation buffer (Diagenode), diluted 1:10 in RPMI with 10% FBS ...
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bioRxiv - Cancer Biology 2022Quote: ... Centrifuged cell lysates were resuspended in shearing buffer and sonicated using the Pico Bioruptor device (Diagenode) for 15 minutes (30s ON/30s OFF) ...
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bioRxiv - Physiology 2020Quote: ... Cells were lysed and DNA-protein complexes were sheared using the Bioruptor Pico (Diagenode, ref B01060010) for 8 minutes ...
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bioRxiv - Genomics 2022Quote: ... Cell lysates were then sonicated 20 times at 4°C (Bioruptor Plus, Diagenode, 30/30 cycles) and centrifuged at 14000 rpm for 1 minute at 4°C to remove cellular debris ...
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bioRxiv - Cancer Biology 2022Quote: ... purified gDNA from M.CviPI-treated cells was fragmented by sonication with a UCD-200 Bioruptor (Diagenode) at high level for 25 sec in 100 μl molecular-grade H2O ...
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bioRxiv - Microbiology 2022Quote: ... Cells were lysed and DNA fragmented (∼500 bp) by sonication in a Bioruptor® Plus (Diagenode) for 48 × 30 s and 30 s cooling ...
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bioRxiv - Cell Biology 2023Quote: ... The cells were incubated on ice for 10 minutes and sonicated using Bioruptor Pico sonicator (Diagenode) for 23 cycles (30s ON/30s OFF) ...
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bioRxiv - Molecular Biology 2023Quote: ... ∼10-20 million cells per shearing tube were sheared by sonication using either the Bioruptor (Diagenode UCD-200 ...
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bioRxiv - Molecular Biology 2024Quote: ... cells lysates were sonicated (15 cycles: 10 sec. on/10 sec. off) using sonicator (Bioruptor, Diagenode) and centrifuged at 12,000 g for 10 min at 4°C ...
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bioRxiv - Immunology 2020Quote: ... Cells were then sonicated for 22 minutes at 30 seconds on/off using the Bioruptor sonicator (Diagenode) and spun down ...
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bioRxiv - Cell Biology 2021Quote: ... Cell lysates were sonicated 18 cycles of 30 s on and 30 s off (Bioruptor Pico, Diagenode) and insoluble material was discarded by centrifugation at 13000rpm for 20 minutes at 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... Cells were disrupted by sonication (two cycles à 20 s at 4 °C) in a BioRuptor (Diagenode), and extracts were cleared by centrifugation at 15,000 x g for 1 min ...
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bioRxiv - Physiology 2022Quote: ... Chromatin from approximately 10 million cells was sheared using a sonication device (Bioruptor Pico, #2013-2019, Diagenode) and optimized to produce ~400bp fragments ...
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bioRxiv - Developmental Biology 2022Quote: ... The cells were lysed and immunoprecipitation were performed using the True MicroChIP kit (Diagenode, AB-002-0016) with the following modifications ...
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bioRxiv - Genomics 2021Quote: ... Cells were then sonicated for 10 cycles of 30 seconds on/off on a Bioruptor Pico (Diagenode) at 4°C ...
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bioRxiv - Genomics 2019Quote: Immunoprecipitations from 105 cells were performed using IP star robot at 4°C (indirect 200µl method, Diagenode) with an anti-BrdU antibody (10μg ...
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bioRxiv - Plant Biology 2022Quote: ... Cells were ruptured by 5 × 15 sec sonication in an ice bath in Bioruptor UCD-200 (Diagenode) at medium intensity with 2 min breaks on ice ...
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bioRxiv - Molecular Biology 2023Quote: ... Chromatin from 10 million cells was sonicated to between 200-600 bp using a Bioruptor Plus (Diagenode). 100µL of Dynabeads Protein G (Invitrogen ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were lysed and chromatin was prepared using the Chromatin EasyShear Kit - High SDS (Diagenode; Cat# C01020012), and shearing of the chromatin was achieved using the Bioruptor with four rounds of ten cycles of 30 seconds “ON„ and 30 seconds “OFF„ at HIGH power setting (position H) ...
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bioRxiv - Genomics 2022Quote: ... U2OS cells treated with Nutlin-3a or DMSO solvent control were sonicated using a Bioruptor (Diagenode, Beligum) for 10 cycles (60 seconds on and 30 seconds off with high intensity ...
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bioRxiv - Genomics 2020Quote: ... One µg per 100,000 cells of the desired antibody (H3K4me3 C15410003, H3K27ac C15410196, H3K27me3 C15410195, all from Diagenode) is added into each well ...
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bioRxiv - Molecular Biology 2021Quote: ... Fixed cells were resuspended in 1 ml RIPA Binding Buffer and placed in the Bioruptor Pico (B01060010, Diagenode) for 10 cycles of 30 seconds on ...
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bioRxiv - Biophysics 2020Quote: ... Cell lysis were performed using EmulsiFlex-C3 (Avestin Europe GmbH, Mannheim, Germany) and Bioruptor UCD-200 (Diagenode, Belgium). Inclusion bodies were recovered by centrifugation of cell lysate at 7,000 x g for 1 hour at 4°C and were resuspended in 20 mL denaturing binding buffer (20 mM Tris-Cl ...
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bioRxiv - Cancer Biology 2022Quote: ... sonicated until the lysates were clear of long DNA and other visible cell debris (Diagenode Bioruptor Plus sonicator). Lysates were processed by the Multi-Enzyme Digestion Filter Aided Sample Preparation (MED FASP ...
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bioRxiv - Cancer Biology 2020Quote: ... The sample was then incubated with antibodies (H3K27ac, Diagenode, C15410196; H3K27me3, Cell Signaling 9733S; H3K4me3, Diagenode C15410003 premium) coupled with protein A and protein G beads (Life Technologies ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were sonicated at high setting with 30 s on/off cycles using a Bioruptor UCD-200 (Diagenode). Lysates were cleared by centrifugation at 14000×g ...
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bioRxiv - Molecular Biology 2023Quote: ... CTCF ChIP-seq samples were prepared with 3 x 106 cells and 1 µg CTCF antibody (Diagenode C15410210). ChIP-seq Dilution Buffer (16.7 mM Tris-HCl pH 8.1 ...
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bioRxiv - Molecular Biology 2021Quote: ... Subsequently, cells were spheroplasted according to the protocol from the Rando lab (Rando, 2010) followed by sonication (Bioruptor, Diagenode: ten cycles ...
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bioRxiv - Genomics 2019Quote: ... Cell clumps were dissociated by mild sonication for 30 s using the medium intensity setting in a Bioruptor (Diagenode). Lysing enzymes from Trichoderma harzianum (Sigma ...
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bioRxiv - Genomics 2021Quote: ... Cells were lysed in two steps with the Diagenode iDeal ChIP-seq kit for transcription factors (Diagenode, Seraing, Belgium).
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bioRxiv - Genomics 2020Quote: ... For single crosslinked cells nuclei were sonicated for 4 cycles of 30s on/30s off using a Picoruptor (Diagenode). Immunoprecipitation was carried out overnight at 4°C using 2 μg of RUNX1 antibody (ab23980 ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were sonicated for 30 cycles at high setting (30s on, 30s off) using a Bioruptor Pico sonicator (Diagenode) and centrifuged at 13000 rpm for 10 min ...
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bioRxiv - Cell Biology 2021Quote: ... Pelleted cells were resuspended in 1 ml 50 mM Tris pH 7.5 and sonicated in a Bioruptor Twin (Diagenode) on LOW 30 sec ON ...
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bioRxiv - Genomics 2020Quote: ... then incubated with specific antibodies (anti-H3K27me3 Cell Signaling Technology 9733, anti-H3K36me2 CST 2901, anti-H3K27ac Diagenode C15410196), 1.5 million cells of sonicated cell lysate ...
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bioRxiv - Cell Biology 2019Quote: ... or Raji iBZLF1 cells induced for 6 h were lysed in RIPA buffer and sheared with the BioRuptor (Diagenode) four times (5 min ...
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bioRxiv - Microbiology 2021Quote: ... Cells were lysed by sonication (5 cycles, each 30 s on and 30 s off) using a Biorupter (Diagenode). A centrifugation step (at 4°C ...
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bioRxiv - Immunology 2020Quote: ... The cells were cross-linked with 1% formaldehyde for 10 minutes at RT and quenched with 2.5 M glycine (Diagenode) for 5 minutes at RT ...
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bioRxiv - Cancer Biology 2023Quote: ... Isolated nuclei from primary fallopian tube secretory epithelial cells bound to concalavin A beads were incubated with primary antibody that detects H3K27ac (0.7μg, Diagenode, RRID:AB_2637079) at 4℃ overnight ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were incubated with mixing for 30 min at 4°C before sonicating with a Bioruptor® Pico (Diagenode). Lysates were then spun for 10 min at 15,000 g ...
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bioRxiv - Genomics 2023Quote: ... 7μL Tri-Methyl-Histone H3 (Lys27) (C36B11) Rabbit mAb (Cell Signalling Technology #9733) or 1μg negative control rabbit IgG (Diagenode) per ChIP reaction ...
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bioRxiv - Genetics 2024Quote: ... Cells were lysed by sonication for 15 cycles (high, 30 seconds on, 30 seconds off) using Bioruptor Plus (Diagenode). After a 15-minute incubation on ice and centrifugation (21,000 ✕ g ...
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bioRxiv - Systems Biology 2024Quote: ... cells were incubated on ice for 30 min and sonicated for five minutes (30 sec on/off, Diagenode Bioruptor). Afterwards ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were sonicated (settings: 3 – 4 x 30 sec on /30 sec off, 4°C, power high; Bioruptor, Diagenode) and lysates were cleared by centrifugation at 16,000 x g at 4°C for 15 min ...