Labshake search
Citations for Diagenode :
51 - 96 of 96 citations for Recombinant Rabbit IL5 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Bioengineering 2024Quote: Unloaded Tn5 transposase proteins were acquired from Diagenode (#C01070010-10). Tn5 transposase was then loaded with oligos containing mosaic ends incorporating 13 base-pair unique molecular identifiers (UMIs ...
-
bioRxiv - Bioengineering 2020Quote: ... rabbit polyclonal anti-Cas9 (C15310258-100, 1:1000, Diagenode, Denville, NJ) and rabbit monoclonal anti-Gapdh (#2118 ...
-
bioRxiv - Systems Biology 2019Quote: ... and rabbit polyclonal anti-H3K4me1 antibody (Cat. No. C15410194, Diagenode Inc.) as described previously (Rochman et ...
-
bioRxiv - Microbiology 2021Quote: ... anti-Histone 4 rabbit (1:2,000; Diagenode Cat. No C15410156-50). HRP-conjugated anti-mouse and anti-rabbit antibodies were used (1:5,000 ...
-
bioRxiv - Molecular Biology 2021Quote: ... The following primary antibodies were used: rabbit MECP2 (Diagenode C15410052, 1:250), chicken Neun (Millipore Sigma MAB377 1:250) ...
-
bioRxiv - Molecular Biology 2021Quote: ... The following primary antibodies were used: rabbit MECP2 (Diagenode C15410052, 1:1000), rabbit OCT1 (Novus Biologicals NBP2-21584 ...
-
bioRxiv - Genomics 2019Quote: Antibodies used for ChIP were rabbit anti H3K9ac (Diagenode Cat# C15410004, RRID:AB_2713905) and rabbit anti-PbHP1 (15) ...
-
bioRxiv - Molecular Biology 2021Quote: ... The following primary antibodies were used: rabbit MECP2 (Diagenode C15410052, 1:1000), rabbit anti-histone H3 (Abcam ab1791 ...
-
bioRxiv - Molecular Biology 2019Quote: ... 1.2 μg total protein was analysed with Cas9 (Diagenode C15310258, 1:400) and GAPDH (EMD MAB374 ...
-
bioRxiv - Cancer Biology 2020Quote: ... followed by immunoprecipation of immunocomplexes with protein-A agarose beads (Diagenode; C03020002) previously blocked with 0.5% BSA for 1 hr at 4°C ...
-
bioRxiv - Developmental Biology 2022Quote: ... Sheared chromatin was thereafter immunoprecipitated with Protein A-coated magnetic beads (Diagenode) preincubated with 4.5 μg/IP MED1 antibody (Bethyl A300-793A ...
-
bioRxiv - Molecular Biology 2023Quote: ... Antibody-bound chromatin was then coupled to protein A beads (Diagenode, C03020002) for three hours at 4° C ...
-
bioRxiv - Genomics 2020Quote: ... and probed with rabbit pAb CRISPR/Cas9 (C15310258, Diagenode, Liège, Belgium, 1:5000), rabbit pAb GFP (ab290 ...
-
bioRxiv - Molecular Biology 2020Quote: ... The Low cell# ChIPkit protein A × 48 was from Diagenode (Denville, NJ, USA). TaqMan probes for mRNA expression and Chemiluminescence western blot detection reagent were from Thermo Fisher Scientific (Waltham ...
-
bioRxiv - Genomics 2021Quote: ... Primary antibodies against the following proteins were applied: H3K4me1 (1:100, C15410037, Diagenode), H3K27ac (1:100 ...
-
bioRxiv - Genetics 2022Quote: ... for immunoprecipitation with 2μg of the following rabbit primary antibodies: anti-H3.3 (Diagenode, C15210011), anti-panH3 (Abcam ...
-
bioRxiv - Molecular Biology 2021Quote: ... The Low Cell# ChIP kit protein A × 48 was from Diagenode (Denville, NJ, USA) and the Chemiluminescence western blot detection reagent was from Thermo Fisher Scientific (Waltham ...
-
bioRxiv - Molecular Biology 2019Quote: ... The immune complexes were purified with DiaMag Protein G coated magnetic beads (C03010021, Diagenode). De-crosslinked DNA was eluted for qPCR to assess changes in Sox2 recruitment to specific areas of interest following 7SK knock down ...
-
bioRxiv - Immunology 2020Quote: 1.5 ml Bioruptor Microtubes were filled with 250 mg of Protein Extraction Beads (Diagenode) and filled with RIPA buffer (supplemented with protease and phosphatase inhibitors) ...
-
bioRxiv - Molecular Biology 2024Quote: ... The sheared chromatin was immunoprecipitated using the DiaMag protein A-coated magnetic beads (Diagenode) and 2 µg of anti-HvHsfA6a antibodies ...
-
bioRxiv - Genetics 2019Quote: ... IRF5 (catalogue number E1N9G Rabbit mAb #13496) and IgG (catalogue number K02041008, Diagenode, Liege, Belgium).
-
bioRxiv - Genomics 2022Quote: ... Cells were lysed and DNA-protein complexes were sheared using the Bioruptor Pico (Diagenode, #B01060010) for 8 minutes ...
-
bioRxiv - Developmental Biology 2023Quote: ... Immunolabeling of Cas9 protein by monoclonal anti-mouse Cas9 antibody at 1:500 (4G10; Diagenode) was blocked in PBS Super Block (37580 ...
-
bioRxiv - Cell Biology 2020Quote: ChIP-seq experiments were carried out using the HighCell# ChIP-Protein G kit (Diagenode, Cat#: C01010063) following the manufacturer’s protocol ...
-
bioRxiv - Developmental Biology 2020Quote: ... The worm suspension containing fixed DNA-protein complexes was sheared using a Bioruptor (Diagenode, Denville, NJ) on the high setting for 20 cycles (30 seconds ON/30 seconds OFF) ...
-
bioRxiv - Physiology 2020Quote: ... Cells were lysed and DNA-protein complexes were sheared using the Bioruptor Pico (Diagenode, ref B01060010) for 8 minutes ...
-
bioRxiv - Cell Biology 2023Quote: ... were used per ChIP and were bound to DiaMag protein G-coated magnetic beads (Diagenode, C03010021) overnight at 4°C with gentle rotation ...
-
bioRxiv - Microbiology 2023Quote: ... the different antibodies were previously incubated at 4°C with protein G-coated magnetic beads (DiaMag, Diagenode), protease inhibitor cocktail and 0.1% BSA ...
-
bioRxiv - Molecular Biology 2023Quote: ... Protein concentrations of lysates were determined after 15 min of sonication (Bioruptor, Diagenode, cycles of 30 s) using the Pierce BCA Protein Assay (Thermo) ...
-
bioRxiv - Molecular Biology 2021Quote: ... Commercial pA-Tn5 (protein A-Tn5) was purchased pre-loaded with sequence adapters from Diagenode (Denville, NJ, USA), and diluted to a working concentration of 1:50 with dilution buffer (40 mM Tris-HCl ...
-
bioRxiv - Cell Biology 2023Quote: ... Lysates were sonicated for 10 minutes using a Bioruptor device together with protein extraction beads (Diagenode, Denville, NJ). Each lysate was diluted with 1080µl 50mM Tris pH7.4 ...
-
bioRxiv - Neuroscience 2024Quote: ... we included a preclearing step by incubating the chromatin lysate with DiaMag Protein A-coated magnetic beads (Diagenode) at 4°C for 1.5 - 2 hours ...
-
bioRxiv - Neuroscience 2022Quote: ... Pre-cleared chromatin lysate was immunoprecipitated by a ChIP-grade rabbit polyclonal antibody against histone H3K9me3 (Cat. # C15410193, Diagenode) with end-over-end rotation (4°C ...
-
bioRxiv - Molecular Biology 2022Quote: ... 2 mg of chromatin lysate was rotated overnight at 4°C with 5 μg antibody: rabbit IgG (Diagenode #C15410206), anti-NIPBL (NIPBL#1 ...
-
bioRxiv - Cancer Biology 2023Quote: ... Samples were taken up in 100 μL LYSE buffer and mixed with 50 mg of protein extraction beads (Diagenode). Samples were sonicated with 10-20 cycles at 30sec ON/OFF in a Diagenode Bioruptor Plus until complete tissue disruption was observed ...
-
bioRxiv - Cell Biology 2023Quote: ... beads were incubated for 30 min at RT with 5 µg of negative control antibody (C-, IgG from healthy rabbits, Diagenode). For immunoprecipitation ...
-
bioRxiv - Neuroscience 2022Quote: ... A modified protocol for on-beads digest (sonication for 10*30s in a Bioruptor sonicator in presence of 50 mg Protein Extraction beads, C20000021, Diagenode) was used for tissue samples followed by a 3.5h digestion step ...
-
bioRxiv - Genomics 2023Quote: ... Preclearing 30μl of Dynabeads protein A/G for 1 h at 4 °C was followed by incubation with antibodies (H3K27ac, Diagenode, 3C15410196 ...
-
bioRxiv - Genomics 2022Quote: ... and 800ul of antibody buffer containing 1ul of primary antibodies (normal rabbit IgG, Santa Cruz Cat no sc-2027, H3K27me3 (Millipore, Catalogue number 07-449, Lot DAM1703508 and H3K9me3 (Diagenode, Catalogue number C15410193 ...
-
bioRxiv - Plant Biology 2021Quote: ... The IP of the methylated DNA was performed overnight at 4°C using Protein A Dynabeads coated with anti-5mC (Diagenode, C15200081) or anti-IgG (Diagenode ...
-
bioRxiv - Cancer Biology 2020Quote: ... samples were incubated by rotating overnight at 4°C with primary antibody or irrelevant IgGs and then incubated with protein-A agarose beads (Diagenode; C03020002) previously blocked with 0.5% BSA for 3 hr at 4 °C with rotation ...
-
bioRxiv - Developmental Biology 2019Quote: ... Embryos were kept on ice and processed directly or frozen at −80C in 20 ml of RNAse/DNAse free PBS supplemented with 1× protein inhibitor (Diagenode, Belgium). Embryos younger than late gastrula (younger than E7.5 ...
-
bioRxiv - Genetics 2021Quote: ... 3 to 7 days later individual colonies were picked and expanded and western blot was used to confirm Cas9 protein expression (Diagenode, C15200203). Cas9 expression in a high expressing line was confirmed by immunohistochemistry (5).
-
bioRxiv - Genomics 2023Quote: ... Preclearing 30μl of Dynabeads protein A/G for 1 h at 4 °C was followed by incubation with antibodies (H3K27ac, Diagenode, 3C15410196; H3K4me3, Diagenode 3C15410003). Reverse-crossed IP sample were pulled down with streptavidin C1 beads (Life Technologies) ...
-
bioRxiv - Microbiology 2023Quote: ... 10 μg total RNA was incubated overnight at 4 °C with Dynabeads™ Protein G magnetic beads pre-bound with anti-Ψ (Diagenode) according to manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2021Quote: ... equal volume of sheared chromatin was incubated overnight with 3 μg of antibody in iC1b buffer with protease inhibitors and BSA and washed DiaMag Protein A-coated magnetic beads (Diagenode Cat# C03010020-220) on a tube rotator at 4°C overnight ...