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Citations for Diagenode :
51 - 70 of 70 citations for Recombinant Mouse CTSB Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2019Quote: ... Crosslinked ChIP of BRD4 from NOD mouse islets was performed using the True MicroChIP kit (Diagenode) and the same procedure was followed for preparing islets ...
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bioRxiv - Microbiology 2023Quote: ... the different antibodies were previously incubated at 4°C with protein G-coated magnetic beads (DiaMag, Diagenode), protease inhibitor cocktail and 0.1% BSA ...
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bioRxiv - Molecular Biology 2023Quote: ... Protein concentrations of lysates were determined after 15 min of sonication (Bioruptor, Diagenode, cycles of 30 s) using the Pierce BCA Protein Assay (Thermo) ...
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bioRxiv - Molecular Biology 2021Quote: ... Commercial pA-Tn5 (protein A-Tn5) was purchased pre-loaded with sequence adapters from Diagenode (Denville, NJ, USA), and diluted to a working concentration of 1:50 with dilution buffer (40 mM Tris-HCl ...
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bioRxiv - Cell Biology 2023Quote: ... Lysates were sonicated for 10 minutes using a Bioruptor device together with protein extraction beads (Diagenode, Denville, NJ). Each lysate was diluted with 1080µl 50mM Tris pH7.4 ...
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bioRxiv - Neuroscience 2024Quote: ... we included a preclearing step by incubating the chromatin lysate with DiaMag Protein A-coated magnetic beads (Diagenode) at 4°C for 1.5 - 2 hours ...
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bioRxiv - Cancer Biology 2023Quote: ... Samples were taken up in 100 μL LYSE buffer and mixed with 50 mg of protein extraction beads (Diagenode). Samples were sonicated with 10-20 cycles at 30sec ON/OFF in a Diagenode Bioruptor Plus until complete tissue disruption was observed ...
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bioRxiv - Neuroscience 2021Quote: ... the adaptor-ligated DNA was used for immunoprecipitation (IP) with a mouse monoclonal anti-5-hydroxymethylcytosine antibody (Diagenode, C15200200). For this ...
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bioRxiv - Cell Biology 2022Quote: ... and DNA fragments (200 - 500 bp) were immunoprecipitated with a mouse monoclonal anti-5hmC antibody (2.5 μg per immunoprecipitation) following the protocol from Diagenode. The DNA-antibody mixture was incubated with magnetic beads overnight at 4°C ...
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bioRxiv - Neuroscience 2022Quote: ... A modified protocol for on-beads digest (sonication for 10*30s in a Bioruptor sonicator in presence of 50 mg Protein Extraction beads, C20000021, Diagenode) was used for tissue samples followed by a 3.5h digestion step ...
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bioRxiv - Genomics 2023Quote: ... Preclearing 30μl of Dynabeads protein A/G for 1 h at 4 °C was followed by incubation with antibodies (H3K27ac, Diagenode, 3C15410196 ...
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bioRxiv - Molecular Biology 2020Quote: ... 1 µg of mouse anti-RNA Polymerase II CTD monoclonal antibody (CMA601)35 or Monoclonal Pol II Antibody – ChIP-seq grade (Diagenode) for RNAPII ...
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bioRxiv - Plant Biology 2021Quote: ... The IP of the methylated DNA was performed overnight at 4°C using Protein A Dynabeads coated with anti-5mC (Diagenode, C15200081) or anti-IgG (Diagenode ...
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bioRxiv - Cancer Biology 2020Quote: ... samples were incubated by rotating overnight at 4°C with primary antibody or irrelevant IgGs and then incubated with protein-A agarose beads (Diagenode; C03020002) previously blocked with 0.5% BSA for 3 hr at 4 °C with rotation ...
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bioRxiv - Developmental Biology 2019Quote: ... Embryos were kept on ice and processed directly or frozen at −80C in 20 ml of RNAse/DNAse free PBS supplemented with 1× protein inhibitor (Diagenode, Belgium). Embryos younger than late gastrula (younger than E7.5 ...
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bioRxiv - Genetics 2021Quote: ... 3 to 7 days later individual colonies were picked and expanded and western blot was used to confirm Cas9 protein expression (Diagenode, C15200203). Cas9 expression in a high expressing line was confirmed by immunohistochemistry (5).
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bioRxiv - Genomics 2023Quote: ... Preclearing 30μl of Dynabeads protein A/G for 1 h at 4 °C was followed by incubation with antibodies (H3K27ac, Diagenode, 3C15410196; H3K4me3, Diagenode 3C15410003). Reverse-crossed IP sample were pulled down with streptavidin C1 beads (Life Technologies) ...
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bioRxiv - Microbiology 2021Quote: 60,000 iATII cells or Calu-3 cells were incubated for 1 h at 4°C with equal protein concentrations of control rabbit IgG (Diagenode, Cat#C15410206) or 1/200 dilution of rabbit anti-ACE2 (Abcam ...
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bioRxiv - Microbiology 2023Quote: ... 10 μg total RNA was incubated overnight at 4 °C with Dynabeads™ Protein G magnetic beads pre-bound with anti-Ψ (Diagenode) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... equal volume of sheared chromatin was incubated overnight with 3 μg of antibody in iC1b buffer with protease inhibitors and BSA and washed DiaMag Protein A-coated magnetic beads (Diagenode Cat# C03010020-220) on a tube rotator at 4°C overnight ...