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Citations for Diagenode :
1 - 50 of 88 citations for Recombinant Human X Linked Inhibitor of Apoptosis AVI tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Dose-dependent effects of histone methyltransferase NSD2 on site-specific double-strand break repairbioRxiv - Cell Biology 2023Quote: ... Cross-linked chromatin was sonicated using a Picoruptor (Diagenode) (10 cycles 30 s ON ...
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bioRxiv - Biochemistry 2024Quote: ... 1 x Halt Protease Inhibitor Cocktail) and water-bath sonicated (Diagenode; 25 cycles, 30 sec on and off). Sheard chromatin was diluted to achieve 0.1 % SDS and incubated with FOXA1 antibody (Novus Biologicals ...
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bioRxiv - Developmental Biology 2023Quote: ... Cross-linked chromatin was subsequently sheared with a Bioruptor (Diagenode) to fragments of 150-400 bp ...
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bioRxiv - Molecular Biology 2023Quote: ... The cross-linked material was sonicated with a Bioruptor (Diagenode), with output set to “high” with 30 s on/off cycles to an average size range of 150–350 bp which was then determined by agarose gel electrophoresis ...
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bioRxiv - Plant Biology 2023Quote: ... The cross-linked chromatin was sheared using Bioruptor sonication system (Diagenode) and sheared chromatin was immunoprecipitated using 1 μg of antibody per reaction ...
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bioRxiv - Cancer Biology 2023Quote: Cells were cross-linked with ChIP Cross-link Gold (Diagenode, # C01019027). After incubation at RT for 30min ...
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bioRxiv - Cancer Biology 2021Quote: ... lysed in 1% SDS containing buffer supplemented with 1x protease and phosphatase inhibitors, and sonicated for 4 x 10 cycles (30 sec on, 30 sec off) in temperature-controlled Bioruptor 300 (Diagenode). Debris was removed by centrifuging at 14,000 rpm at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... Cell pellet was resuspended in ice-cold lysis buffer (1xPBS, 2 mM PMSF, 2 mM DTT, 1 x protease inhibitors (Pierce)) and sonicated (Bioruptor Pico, Diagenode) at 4LJ with alternating 10 s burst/10 s break ...
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bioRxiv - Plant Biology 2021Quote: ... Cross-linked chromatin was sonicated using a water bath Bioruptor Pico (Diagenode; 30sec ON/30sec OFF pulses ...
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bioRxiv - Genetics 2023Quote: ... the cross-linked chromatin was immunoprecipitated with ∼1μg of CTCF antibody (Diagenode), H3K4me1 antibody (Diagenode) ...
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bioRxiv - Microbiology 2023Quote: ... 5 μg of His-tagged H2A:H2B or His-H3:Flag-H4 (Diagenode) and 2 μg of Flag-hSpt16 were added to 250 μl of binding buffer (50 mM Tris-HCl pH 7.4 ...
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bioRxiv - Molecular Biology 2022Quote: ... 100 mM TEAB and 1 x Halt protease inhibitor cocktail) and sonication (3 × 5 min cycles of 30s on/off; Diagenode Bioruptor sonication device). Extracted proteins protein were digested to peptides with in-solution digestion performed at the Turku Proteomics Facility ...
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bioRxiv - Plant Biology 2020Quote: ... Cross-linked chromatin was sonicated using a water bath Bioruptor UCD-200 (Diagenode, Liège, Belgium) (15s on/15s off pulses ...
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bioRxiv - Cancer Biology 2022Quote: ... The cross-linked cells were then lysed and sonicated using Bioruptor Plus sonication device (Diagenode) to obtain ∼300 bp chromatin ...
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bioRxiv - Genetics 2019Quote: ... cross-linked pellets were thawed and processed using the iDeal® ChIP-seq kit for histones (Diagenode) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... with protease inhibitors (Diagenode). Chromatin was then precleared with MagneGST magnetic beads (Promega ...
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bioRxiv - Immunology 2020Quote: ... The cells were cross-linked with 1% formaldehyde for 10 minutes at RT and quenched with 2.5 M glycine (Diagenode) for 5 minutes at RT ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 % Triton X-100) supplemented with 1 x HALT in a 15 ml Bioruptor tube containing one scoop of sonication beads (Diagenode) and sonicated in Bioruptor Pico (Diagenode ...
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bioRxiv - Cell Biology 2022Quote: ... sonicated 5 x 30 sec in a Bioruptor (Diagenode) at 4 °C ...
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bioRxiv - Cell Biology 2023Quote: ... sonicated a minimum of 3 x 30 seconds (Diagenode sonicator), were optionally reduced with 100 mM dithiothreitol ...
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bioRxiv - Genetics 2020Quote: ... 1.0% Triton X-100) was sonicated using a BioRuptor sonicator (Diagenode), followed by centrifugation at 16,000×g for 20 min at 4°C ...
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bioRxiv - Molecular Biology 2022Quote: ... Protease inhibitor and RNase inhibitor) and sonicated for 10 cycles (30 secs on 30 secs off) on a Bioruptor (Diagenode) sonicator ...
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bioRxiv - Molecular Biology 2021Quote: ... and sonicated using a Bioruptor (Diagenode, medium power, 3 x 15 sec). Protein concentration was measured using the Bradford assay (Bio-Rad) ...
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bioRxiv - Molecular Biology 2020Quote: ... 1% Triton X-100) and subjected to sonication using Bioruptor Standard (Diagenode). After centrifugation ...
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bioRxiv - Plant Biology 2019Quote: ... Five µg of genomic DNA were sonicated (Diagenode Bioruptor: 18 x 30 s) and denatured 10 min at 95°C in Buffer 1 (10 mM Tris HCl pH 7.5 ...
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bioRxiv - Plant Biology 2020Quote: ... and protease inhibitor cocktail) and sonicated with a Bioruptor (Diagenode). The nuclei lysate was precipitated with anti-Flag (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2021Quote: ... 1X Placental Rnase inhibitor) and sonicate using Bioruptor 300 (Diagenode) at high intensity for 60 cycles with 15 seconds ON and 45 seconds OFF each cycle ...
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bioRxiv - Cell Biology 2021Quote: ... 1X Placental Rnase inhibitor] and sonicate using Bioruptor 300 (Diagenode) at high intensity for 60 cycles with 15 seconds ON and 45 seconds OFF each cycle ...
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bioRxiv - Molecular Biology 2022Quote: ... protease inhibitor cocktail) and sonicated in the Bioruptor Pico (Diagenode) on low power at 15 seconds on 30 seconds off cycles until at least 70% cell membranes lysed and intact nuclei were visible ...
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bioRxiv - Immunology 2019Quote: ... 3 µL protease inhibitor cocktail 1µg of H3K27ac antibody (Diagenode) and incubated overnight at 4 ºC with rotation ...
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bioRxiv - Genetics 2019Quote: ... we used ChIP grade antibodies specific to human H3K27ac histones (Diagenode). A negative control undergoing no IP (input ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were then sonicated (Bioruptor plus, Diagenode, 10 x 30s/30s ON/OFF cycles) and heated up to 37°C for 2 h while shaking (800 rpm ...
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bioRxiv - Biochemistry 2021Quote: ... protease inhibitor cocktail) and sonicated in 4°C using Bioruptor (Diagenode) until lysate is clear ...
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bioRxiv - Genomics 2019Quote: ... 10 mM EDTA (2 x 107 cell/ml) and sonicated in a Bioruptor device (Diagenode) for 25 min at 30 s intervals ...
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bioRxiv - Genetics 2021Quote: ... 7.5⍰x⍰106 cells were then homogenized and sheared by sonication using a Bioruptor (Diagenode) at high setting for 15⍰min ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were sonicated in 6 x 30 second intervals using a Bioruptor 300 Machine (Diagenode), then centrifuged for 10 minutes at 10,000g at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... protease inhibitor cocktail (Pierce #A32953) DNA was sheared using Biorup-tor (Diagenode), 20 cycles of 30 seconds on/30 seconds off ...
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bioRxiv - Neuroscience 2019Quote: ... 2.5X protease inhibitors EDTA-free) and sonicated with Bioruptor Power-up (Diagenode) to shear DNA into 150-700 bp fragments ...
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bioRxiv - Microbiology 2023Quote: ... and resuspended in 500 μl of cold PBS + protease inhibitor (Diagenode kit). Nuclei preparation ...
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bioRxiv - Genetics 2023Quote: ... 0.1 % SDS) containing Complete proteinase inhibitor cocktail and sonicated with Picoruptor (Diagenode). After sonication debris was removed by centrifugation at 20,000 g for 10 min ...
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bioRxiv - Plant Biology 2019Quote: ... and sonicated 2 cycle x 30 second ON/ 30 second OFF using Bioruptor® Plus (Diagenode). After removal of cell debris by centrifugation (5 min ...
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bioRxiv - Genetics 2019Quote: ... Chromatin was sheared 10 x 30s ON/30s OFF with a Bioruptor Pico (Diagenode, Denville, NJ). For ChIP-qPCR ...
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bioRxiv - Developmental Biology 2020Quote: ... 10mM EDTA ph8 and proteinase inhibitors) in a Bioruptor Pico sonicating device (Diagenode). All ChIPs were incubated overnight with the respective antibodies (CTCF Active Motif 61311 ...
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bioRxiv - Genomics 2023Quote: ... the nuclei pellet was resuspended in 1mL of shearing buffer + protease inhibitor (Diagenode), incubated for 10 mins on ice ...
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bioRxiv - Genomics 2024Quote: ... proteinase inhibitor cocktail) and sonicated to fragments about 300 - 500 bp (Bioruptor, Diagenode). Dynabeads Protein A was washed twice with ChIP Buffer (10mM Tris-HCl pH7.5 ...
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bioRxiv - Genetics 2022Quote: ... Immunoprecipitation was performed using 10 μg of chromatin and 2.5 μg of human antibody (H3K4me3 [RRID:AB_2616052, Diagenode C15410003], H3K4me1 [RRID:AB_306847, Abcam ab8895], and H3K27ac [RRID:AB_2637079, Diagenode C15410196]). Final DNA purification was performed using the GeneJET PCR Purification Kit (Thermo Scientific ...
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bioRxiv - Molecular Biology 2019Quote: ... 150 mM NaCl and protease inhibitors) with a Bioruptor Sonication System (Diagenode, UCD-200). 20 μg of sonicated chromatin per sample was incubated overnight with 2-5 μg of antibody/protein A magnetic beads complexes ...
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The epigenomic landscape regulating organogenesis in human embryos linked to developmental disordersbioRxiv - Genomics 2019Quote: ... 1% SDS and protease inhibitor cocktail) and sonicated under prior optimised conditions (Diagenode Bioruptor). Sufficient sample was prepared to allow in parallel immunoprecipitation for H3K4me3 ...
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bioRxiv - Plant Biology 2023Quote: ... Protease Inhibitor Cocktail) and sonicated by using a Bioruptor® Plus sonication device (Diagenode) to obtain the desired DNA fragment size (enriched at 500 bp) ...
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bioRxiv - Molecular Biology 2023Quote: ... CTCF ChIP-seq samples were prepared with 3 x 106 cells and 1 µg CTCF antibody (Diagenode C15410210). ChIP-seq Dilution Buffer (16.7 mM Tris-HCl pH 8.1 ...