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Citations for Diagenode :
1 - 50 of 52 citations for Recombinant Human TNFSF18 Protein Fc Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... 5 μg of His-tagged H2A:H2B or His-H3:Flag-H4 (Diagenode) and 2 μg of Flag-hSpt16 were added to 250 μl of binding buffer (50 mM Tris-HCl pH 7.4 ...
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bioRxiv - Genetics 2019Quote: ... we used ChIP grade antibodies specific to human H3K27ac histones (Diagenode). A negative control undergoing no IP (input ...
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bioRxiv - Genomics 2021Quote: ... Dynal Protein A magnetic beads (Diagenode) and ChIP-graded anti-myc antibody (ab9132 ...
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bioRxiv - Biochemistry 2023Quote: ... Unblocked protein A beads (Diagenode, C03020003) were used to pulldown the antibody-protein complexes ...
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bioRxiv - Cancer Biology 2021Quote: ... The DNA-protein-antibody complexes were captured by DiaMag protein A-coated magnetic beads (Diagenode; Cat#: C03010020) by incubating at 4 °C for 2 hours ...
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bioRxiv - Cancer Biology 2021Quote: ... The DNA-protein-antibody complexes were captured by DiaMag protein A-coated magnetic beads (Diagenode; Cat#: C03010020) by incubating at 4 °C for 2 hours ...
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bioRxiv - Neuroscience 2023Quote: ... and protein extraction beads (Diagenode, NJ, USA) according to the manufacturer instructions ...
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bioRxiv - Neuroscience 2022Quote: ... Chromatin-protein complexes were immunoprecipitated with protein A/G magnetic beads and washed sequentially according to the manufacturer (Diagenode, C01010055). DNA fragments were then purified using IPure beads v2 (Diagenode ...
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bioRxiv - Developmental Biology 2019Quote: ... overnight and Protein A magnetic beads (Diagenode C03010020) the following day for 2 hours ...
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bioRxiv - Cancer Biology 2022Quote: ... homogenization was performed using protein extraction beads (Diagenode) and sonication in the lysis buffer followed by centrifugation to remove solid remains (Diagenode Bioruptor Plus sonicator manufacturer’s protocol for protein extraction from tissue fragments) ...
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bioRxiv - Developmental Biology 2019Quote: ... ChIP was performed using Protein G beads (Diagenode) O/N using a H3K27ac antibody (Active Motif ...
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bioRxiv - Microbiology 2023Quote: ... magnetic beads coated with DiaMag protein A (Diagenode), and antibodies against epitope tags (HA or MYC ...
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bioRxiv - Plant Biology 2021Quote: ... Protein extract was sonicated using a cooled Biorupter (Diagenode) for 10 minutes using high power with 30 seconds on 30 seconds off cycle ...
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bioRxiv - Plant Biology 2020Quote: ... magnetic beads (DiaMag protein A-coated magnetic beads: Diagenode) were incubated with each antibody overnight ...
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bioRxiv - Molecular Biology 2023Quote: ... Total proteins were sonicated on a Bioruptor Plus (Diagenode) (position high ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein extracts were finally sonicated in a Bioruptor (Diagenode) for 3 cycles at high intensity ...
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bioRxiv - Genetics 2022Quote: ... Immunoprecipitation was performed using 10 μg of chromatin and 2.5 μg of human antibody (H3K4me3 [RRID:AB_2616052, Diagenode C15410003], H3K4me1 [RRID:AB_306847, Abcam ab8895], and H3K27ac [RRID:AB_2637079, Diagenode C15410196]). Final DNA purification was performed using the GeneJET PCR Purification Kit (Thermo Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... in presence of 50 mg Protein Extraction beads (C20000021, Diagenode); samples were trypsin digested for 2 h 30 min ...
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bioRxiv - Bioengineering 2024Quote: Unloaded Tn5 transposase proteins were acquired from Diagenode (#C01070010-10). Tn5 transposase was then loaded with oligos containing mosaic ends incorporating 13 base-pair unique molecular identifiers (UMIs ...
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bioRxiv - Genetics 2022Quote: ... Immunoprecipitation was performed using 10 μg of chromatin and 2.5 μg of human antibody (H3K4me3 [RRID:AB_2616052, Diagenode C15410003] ...
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bioRxiv - Molecular Biology 2019Quote: ... 1.2 μg total protein was analysed with Cas9 (Diagenode C15310258, 1:400) and GAPDH (EMD MAB374 ...
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bioRxiv - Cancer Biology 2020Quote: ... followed by immunoprecipation of immunocomplexes with protein-A agarose beads (Diagenode; C03020002) previously blocked with 0.5% BSA for 1 hr at 4°C ...
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bioRxiv - Developmental Biology 2022Quote: ... Sheared chromatin was thereafter immunoprecipitated with Protein A-coated magnetic beads (Diagenode) preincubated with 4.5 μg/IP MED1 antibody (Bethyl A300-793A ...
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bioRxiv - Molecular Biology 2023Quote: ... Antibody-bound chromatin was then coupled to protein A beads (Diagenode, C03020002) for three hours at 4° C ...
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bioRxiv - Molecular Biology 2020Quote: ... The Low cell# ChIPkit protein A × 48 was from Diagenode (Denville, NJ, USA). TaqMan probes for mRNA expression and Chemiluminescence western blot detection reagent were from Thermo Fisher Scientific (Waltham ...
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bioRxiv - Genomics 2021Quote: ... Primary antibodies against the following proteins were applied: H3K4me1 (1:100, C15410037, Diagenode), H3K27ac (1:100 ...
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bioRxiv - Molecular Biology 2021Quote: ... The Low Cell# ChIP kit protein A × 48 was from Diagenode (Denville, NJ, USA) and the Chemiluminescence western blot detection reagent was from Thermo Fisher Scientific (Waltham ...
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bioRxiv - Molecular Biology 2019Quote: ... The immune complexes were purified with DiaMag Protein G coated magnetic beads (C03010021, Diagenode). De-crosslinked DNA was eluted for qPCR to assess changes in Sox2 recruitment to specific areas of interest following 7SK knock down ...
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bioRxiv - Immunology 2020Quote: 1.5 ml Bioruptor Microtubes were filled with 250 mg of Protein Extraction Beads (Diagenode) and filled with RIPA buffer (supplemented with protease and phosphatase inhibitors) ...
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bioRxiv - Molecular Biology 2024Quote: ... The sheared chromatin was immunoprecipitated using the DiaMag protein A-coated magnetic beads (Diagenode) and 2 µg of anti-HvHsfA6a antibodies ...
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bioRxiv - Developmental Biology 2023Quote: ... Immunolabeling of Cas9 protein by monoclonal anti-mouse Cas9 antibody at 1:500 (4G10; Diagenode) was blocked in PBS Super Block (37580 ...
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bioRxiv - Genomics 2022Quote: ... Cells were lysed and DNA-protein complexes were sheared using the Bioruptor Pico (Diagenode, #B01060010) for 8 minutes ...
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bioRxiv - Cell Biology 2020Quote: ChIP-seq experiments were carried out using the HighCell# ChIP-Protein G kit (Diagenode, Cat#: C01010063) following the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2020Quote: ... The worm suspension containing fixed DNA-protein complexes was sheared using a Bioruptor (Diagenode, Denville, NJ) on the high setting for 20 cycles (30 seconds ON/30 seconds OFF) ...
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bioRxiv - Physiology 2020Quote: ... Cells were lysed and DNA-protein complexes were sheared using the Bioruptor Pico (Diagenode, ref B01060010) for 8 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... were used per ChIP and were bound to DiaMag protein G-coated magnetic beads (Diagenode, C03010021) overnight at 4°C with gentle rotation ...
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bioRxiv - Microbiology 2023Quote: ... the different antibodies were previously incubated at 4°C with protein G-coated magnetic beads (DiaMag, Diagenode), protease inhibitor cocktail and 0.1% BSA ...
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bioRxiv - Molecular Biology 2023Quote: ... Protein concentrations of lysates were determined after 15 min of sonication (Bioruptor, Diagenode, cycles of 30 s) using the Pierce BCA Protein Assay (Thermo) ...
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bioRxiv - Molecular Biology 2021Quote: ... Commercial pA-Tn5 (protein A-Tn5) was purchased pre-loaded with sequence adapters from Diagenode (Denville, NJ, USA), and diluted to a working concentration of 1:50 with dilution buffer (40 mM Tris-HCl ...
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bioRxiv - Cell Biology 2023Quote: ... Lysates were sonicated for 10 minutes using a Bioruptor device together with protein extraction beads (Diagenode, Denville, NJ). Each lysate was diluted with 1080µl 50mM Tris pH7.4 ...
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bioRxiv - Neuroscience 2024Quote: ... we included a preclearing step by incubating the chromatin lysate with DiaMag Protein A-coated magnetic beads (Diagenode) at 4°C for 1.5 - 2 hours ...
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bioRxiv - Cancer Biology 2023Quote: ... Samples were taken up in 100 μL LYSE buffer and mixed with 50 mg of protein extraction beads (Diagenode). Samples were sonicated with 10-20 cycles at 30sec ON/OFF in a Diagenode Bioruptor Plus until complete tissue disruption was observed ...
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bioRxiv - Neuroscience 2022Quote: ... A modified protocol for on-beads digest (sonication for 10*30s in a Bioruptor sonicator in presence of 50 mg Protein Extraction beads, C20000021, Diagenode) was used for tissue samples followed by a 3.5h digestion step ...
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bioRxiv - Genomics 2023Quote: ... Preclearing 30μl of Dynabeads protein A/G for 1 h at 4 °C was followed by incubation with antibodies (H3K27ac, Diagenode, 3C15410196 ...
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bioRxiv - Plant Biology 2021Quote: ... The IP of the methylated DNA was performed overnight at 4°C using Protein A Dynabeads coated with anti-5mC (Diagenode, C15200081) or anti-IgG (Diagenode ...
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bioRxiv - Cancer Biology 2020Quote: ... samples were incubated by rotating overnight at 4°C with primary antibody or irrelevant IgGs and then incubated with protein-A agarose beads (Diagenode; C03020002) previously blocked with 0.5% BSA for 3 hr at 4 °C with rotation ...
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bioRxiv - Developmental Biology 2019Quote: ... Embryos were kept on ice and processed directly or frozen at −80C in 20 ml of RNAse/DNAse free PBS supplemented with 1× protein inhibitor (Diagenode, Belgium). Embryos younger than late gastrula (younger than E7.5 ...
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bioRxiv - Genetics 2021Quote: ... 3 to 7 days later individual colonies were picked and expanded and western blot was used to confirm Cas9 protein expression (Diagenode, C15200203). Cas9 expression in a high expressing line was confirmed by immunohistochemistry (5).
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bioRxiv - Genomics 2023Quote: ... Preclearing 30μl of Dynabeads protein A/G for 1 h at 4 °C was followed by incubation with antibodies (H3K27ac, Diagenode, 3C15410196; H3K4me3, Diagenode 3C15410003). Reverse-crossed IP sample were pulled down with streptavidin C1 beads (Life Technologies) ...
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bioRxiv - Microbiology 2021Quote: 60,000 iATII cells or Calu-3 cells were incubated for 1 h at 4°C with equal protein concentrations of control rabbit IgG (Diagenode, Cat#C15410206) or 1/200 dilution of rabbit anti-ACE2 (Abcam ...