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Citations for Diagenode :
1 - 50 of 445 citations for Rat Segment polarity protein dishevelled homolog DVL 1 DVL1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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The G2-phase enriched lncRNA SNHG26 is necessary for proper cell cycle progression and proliferationbioRxiv - Molecular Biology 2021Quote: ... and 2.5 μg anti-enhancer of zeste homolog 2 (EZH2) (Diagenode, C15200180). Antibody-protein complexes were immunoprecipitated with protein A/G Dynabeads for 3 hours at 4°C with rotation ...
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bioRxiv - Molecular Biology 2022Quote: ... incubated in blocking buffer with 1:500 rat anti-5hmC (Diagenode) overnight at 4°C and followed by incubation with 1:1000 anti-rat IgG HRP (ab6734 ...
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bioRxiv - Molecular Biology 2021Quote: ... The Low Cell# ChIP kit protein A × 48 was from Diagenode (Denville, NJ, USA) and the Chemiluminescence western blot detection reagent was from Thermo Fisher Scientific (Waltham ...
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bioRxiv - Molecular Biology 2019Quote: ... 1.2 μg total protein was analysed with Cas9 (Diagenode C15310258, 1:400) and GAPDH (EMD MAB374 ...
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bioRxiv - Genomics 2021Quote: ... Primary antibodies against the following proteins were applied: H3K4me1 (1:100, C15410037, Diagenode), H3K27ac (1:100 ...
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bioRxiv - Cell Biology 2020Quote: ChIP-seq experiments were carried out using the HighCell# ChIP-Protein G kit (Diagenode, Cat#: C01010063) following the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2023Quote: ... Immunolabeling of Cas9 protein by monoclonal anti-mouse Cas9 antibody at 1:500 (4G10; Diagenode) was blocked in PBS Super Block (37580 ...
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bioRxiv - Genomics 2023Quote: ... Preclearing 30μl of Dynabeads protein A/G for 1 h at 4 °C was followed by incubation with antibodies (H3K27ac, Diagenode, 3C15410196 ...
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bioRxiv - Genomics 2021Quote: ... Dynal Protein A magnetic beads (Diagenode) and ChIP-graded anti-myc antibody (ab9132 ...
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bioRxiv - Biochemistry 2023Quote: ... Unblocked protein A beads (Diagenode, C03020003) were used to pulldown the antibody-protein complexes ...
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bioRxiv - Developmental Biology 2019Quote: ... Embryos were kept on ice and processed directly or frozen at −80C in 20 ml of RNAse/DNAse free PBS supplemented with 1× protein inhibitor (Diagenode, Belgium). Embryos younger than late gastrula (younger than E7.5 ...
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bioRxiv - Genomics 2023Quote: ... Preclearing 30μl of Dynabeads protein A/G for 1 h at 4 °C was followed by incubation with antibodies (H3K27ac, Diagenode, 3C15410196; H3K4me3, Diagenode 3C15410003). Reverse-crossed IP sample were pulled down with streptavidin C1 beads (Life Technologies) ...
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bioRxiv - Cancer Biology 2021Quote: ... The DNA-protein-antibody complexes were captured by DiaMag protein A-coated magnetic beads (Diagenode; Cat#: C03010020) by incubating at 4 °C for 2 hours ...
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bioRxiv - Cancer Biology 2021Quote: ... The DNA-protein-antibody complexes were captured by DiaMag protein A-coated magnetic beads (Diagenode; Cat#: C03010020) by incubating at 4 °C for 2 hours ...
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bioRxiv - Microbiology 2021Quote: 60,000 iATII cells or Calu-3 cells were incubated for 1 h at 4°C with equal protein concentrations of control rabbit IgG (Diagenode, Cat#C15410206) or 1/200 dilution of rabbit anti-ACE2 (Abcam ...
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bioRxiv - Neuroscience 2023Quote: ... and protein extraction beads (Diagenode, NJ, USA) according to the manufacturer instructions ...
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bioRxiv - Neuroscience 2022Quote: ... Chromatin-protein complexes were immunoprecipitated with protein A/G magnetic beads and washed sequentially according to the manufacturer (Diagenode, C01010055). DNA fragments were then purified using IPure beads v2 (Diagenode ...
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Redundant and specific roles of cohesin STAG subunits in chromatin looping and transcription controlbioRxiv - Cell Biology 2019Quote: ... The eluates were diluted 1:50 with ChIP buffer from the One Day ChIP kit (Diagenode) and subsequently subjected to a second ChIP in accordance with the One Day ChIP kit manual but performing an overnight antibody incubation under rotation at 4°C ...
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bioRxiv - Developmental Biology 2019Quote: ... overnight and Protein A magnetic beads (Diagenode C03010020) the following day for 2 hours ...
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bioRxiv - Cancer Biology 2022Quote: ... homogenization was performed using protein extraction beads (Diagenode) and sonication in the lysis buffer followed by centrifugation to remove solid remains (Diagenode Bioruptor Plus sonicator manufacturer’s protocol for protein extraction from tissue fragments) ...
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bioRxiv - Developmental Biology 2019Quote: ... ChIP was performed using Protein G beads (Diagenode) O/N using a H3K27ac antibody (Active Motif ...
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bioRxiv - Microbiology 2023Quote: ... magnetic beads coated with DiaMag protein A (Diagenode), and antibodies against epitope tags (HA or MYC ...
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bioRxiv - Neuroscience 2023Quote: ... RNA samples were incubated overnight at 4°C with 12.5 µg of anti-5-hmC antibody (Diagenode rat monoclonal MAb-633HMC) or without antibody as negative control in IP buffer (750 mM NaCl ...
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bioRxiv - Molecular Biology 2020Quote: ... Libraries were generated from 1 ng of DNA using the MicroPlex Library Preparation Kit v2 (C05010012, Diagenode) according to manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: ... Protein extract was sonicated using a cooled Biorupter (Diagenode) for 10 minutes using high power with 30 seconds on 30 seconds off cycle ...
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bioRxiv - Plant Biology 2020Quote: ... magnetic beads (DiaMag protein A-coated magnetic beads: Diagenode) were incubated with each antibody overnight ...
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bioRxiv - Molecular Biology 2023Quote: ... Total proteins were sonicated on a Bioruptor Plus (Diagenode) (position high ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein extracts were finally sonicated in a Bioruptor (Diagenode) for 3 cycles at high intensity ...
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bioRxiv - Genomics 2020Quote: Preparation of reagents: ChIP Wash buffers 1 to 4 (from iDeal ChIP-seq kit for histones, Diagenode C01010173) are aliquoted into quarter module reservoirs divided by length ...
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bioRxiv - Neuroscience 2023Quote: ... in presence of 50 mg Protein Extraction beads (C20000021, Diagenode); samples were trypsin digested for 2 h 30 min ...
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bioRxiv - Bioengineering 2024Quote: Unloaded Tn5 transposase proteins were acquired from Diagenode (#C01070010-10). Tn5 transposase was then loaded with oligos containing mosaic ends incorporating 13 base-pair unique molecular identifiers (UMIs ...
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bioRxiv - Genomics 2023Quote: ... The immunoprecipitated DNA and 1% of the total input were reverse-crosslinked and eluted using the iPure v2 kit (Diagenode). For library preparation ...
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bioRxiv - Cancer Biology 2020Quote: ... followed by immunoprecipation of immunocomplexes with protein-A agarose beads (Diagenode; C03020002) previously blocked with 0.5% BSA for 1 hr at 4°C ...
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bioRxiv - Developmental Biology 2022Quote: ... Sheared chromatin was thereafter immunoprecipitated with Protein A-coated magnetic beads (Diagenode) preincubated with 4.5 μg/IP MED1 antibody (Bethyl A300-793A ...
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bioRxiv - Molecular Biology 2023Quote: ... Antibody-bound chromatin was then coupled to protein A beads (Diagenode, C03020002) for three hours at 4° C ...
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bioRxiv - Molecular Biology 2023Quote: MeDIP-Seq libraries for Ion Torrent semiconductor sequencing were performed on the Ion Torrent PGM using a modified protocol from Ion Plus Fragment Library kit (Catalog # 4471252) combined with a 5-hydroxymethylcytosine (5hmC Kit, Catalog # AF-110–0016) immunoprecipitation kit (Diagenode) according to a previously optimized protocol (Guerrero-Bosagna & Jensen ...
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bioRxiv - Immunology 2022Quote: ... iPure kit v2 (Diagenode) was used ...
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bioRxiv - Genomics 2019Quote: ... MethylCap kit (Diagenode, C02020010) was employed to obtain the methylated DNA ...
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bioRxiv - Immunology 2021Quote: ... iPure kit v2 (Diagenode) was used ...
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bioRxiv - Molecular Biology 2020Quote: ... The Low cell# ChIPkit protein A × 48 was from Diagenode (Denville, NJ, USA). TaqMan probes for mRNA expression and Chemiluminescence western blot detection reagent were from Thermo Fisher Scientific (Waltham ...
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bioRxiv - Genomics 2022Quote: ... libraries were constructed with 200 ng input DNA from each sample (n = 24; Table 1) using the Premium RRBS Kit (Diagenode, Cat. No. C02030032) according to manufacturer instructions ...
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bioRxiv - Plant Biology 2023Quote: ... DNA was purified according to the IPure kit v2 Kit manual (Diagenode, C03010015). The amount of immunoprecipitated DNA was quantified by qPCR ...
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bioRxiv - Molecular Biology 2019Quote: ... The immune complexes were purified with DiaMag Protein G coated magnetic beads (C03010021, Diagenode). De-crosslinked DNA was eluted for qPCR to assess changes in Sox2 recruitment to specific areas of interest following 7SK knock down ...
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bioRxiv - Immunology 2020Quote: 1.5 ml Bioruptor Microtubes were filled with 250 mg of Protein Extraction Beads (Diagenode) and filled with RIPA buffer (supplemented with protease and phosphatase inhibitors) ...
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bioRxiv - Molecular Biology 2024Quote: ... The sheared chromatin was immunoprecipitated using the DiaMag protein A-coated magnetic beads (Diagenode) and 2 µg of anti-HvHsfA6a antibodies ...
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bioRxiv - Genomics 2019Quote: ... Auto hMeDIP kit (Diagenode C02010033) was used according to manufacturer instructions ...
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bioRxiv - Immunology 2019Quote: ... using LowCell# ChIP kit (Diagenode), with 1μg of antibody against ARNTL (Abcam ...
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bioRxiv - Microbiology 2022Quote: ... or hMeDIP kit (Diagenode, C02010031). Quantification of precipitated DNA was determined using real-time PCR and the delta Ct method for relative quantitation (ABI 7900HT Fast Real-Time PCR System).
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bioRxiv - Genomics 2022Quote: ... Cells were lysed and DNA-protein complexes were sheared using the Bioruptor Pico (Diagenode, #B01060010) for 8 minutes ...
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bioRxiv - Molecular Biology 2021Quote: ... 1μL SYBR green 10X (Diagenode kit), 1.3 μL of molecular biology grade water and 5μL of 2xPCR NEB master mix ...