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Citations for Diagenode :
551 - 569 of 569 citations for Rat Nuclear Factor Of Activated T Cells 5 NFAT5 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... the crosslinked cells were resuspended in 2 mL of lysis buffer and sonicated (in a cold room) by using a Bioruptor (Diagenode) with the following parameters ...
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bioRxiv - Molecular Biology 2020Quote: ... with minor modifications: Cells were resuspended in PBS at 4°C after flow cytometry sorting and incubated with ChIP Crosslink Gold (Diagenode #C01019027 ...
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bioRxiv - Molecular Biology 2019Quote: ... The digestion was then stopped with 5mM EDTA and the digested chromatin was solubilized in 1% SDS and three pulses of 30sec sonication using a Bioruptor ultrasonic cell disruptor (Diagenode). 200µg of pre-cleared chromatin was immunopurified with incubation of 30µl of Anti-FLAG M2 Affinity Gel (Sigma ...
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bioRxiv - Developmental Biology 2019Quote: ... Cells were lysed and homogenised using a Dounce homogeniser and the lysate was sonicated in a Bioruptor® Plus (Diagenode), on high for 3 × 5 min (30 sec on ...
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bioRxiv - Cell Biology 2022Quote: ... cells were sonicated for 10 cycles of 30 sec “on” and 30 sec “off” at 4°C with Bioruptor (Diagenode) and the protein concentration was measured using Bradford protein assay (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2022Quote: ... Immunoprecipitation was done on chromatin corresponding to the remaining 10 000 cells using antibodies against H3K4me3 (Diagenode, cat no. C15410003) – 10% of the chromatin was used for input ...
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bioRxiv - Molecular Biology 2022Quote: ... Cross-linked cell lysates were solubilized using a multi-beads shocker (Yasui Kikai) and sonicated using a Bioruptor UCD-200 system (Diagenode). The sheared samples were centrifuged at 20,000 g for 10 min at 4°C ...
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bioRxiv - Immunology 2023Quote: ... Cells were then lysed to isolate chromatin and sonicated for 10-20 min (depending on cell type) using a Bioruptor (Diagenode). Fragmentation of sonicated chromatin to lengths of 200-500 bp was confirmed by agarose gel electrophoresis.
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bioRxiv - Biochemistry 2023Quote: ... Cell lysis was obtained by pulsed ultrasonication (10 cycles, 30” On, 30” Off, at High intensity using Diagenode Bioruptor 300), complete lysis was monitored by phase contrast microscopy ...
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bioRxiv - Genomics 2020Quote: ... The sheared chromatin derived from one million cells was subjected to magnetic immunoprecipitation and tagmentation using an SX-8G IP-STAR® Compact Automated System (Diagenode) with the anti-GATA6 antibody (D61E4 ...
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bioRxiv - Cancer Biology 2019Quote: ... 1 × 107 cells / immunoprecipitation were fixed for 30 minutes (min) at room temperature (RT) using Diagenode Crosslink Gold (Diagenode, Liège, Belgium). After two washing steps with PBS (Thermo Fisher Scientific) ...
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bioRxiv - Systems Biology 2021Quote: SARS-CoV-2 infected A549-ACE2 cell lysates were sonicated (10 min, 4°C, 30 sec on, 30 sec off, low settings; Bioruptor, Diagenode SA). Protein concentration was adjusted based on Pierce660 assay supplemented with ionic detergent compatibility reagent ...
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bioRxiv - Genomics 2021Quote: ... pH 7.4) aided by homogenization by passing the cells through various gouge needles and sonication using the Bioruptor® sonication system (Diagenode) for 15 cycles of 30 s ON / 30 s OFF at high setting ...
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bioRxiv - Genomics 2022Quote: ... and cell lysates were sonicated for six cycles (30 sec on/30 sec off) using the Bioruptor® Pico (B01060001, Diagenode). Per RIME ...
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bioRxiv - Cell Biology 2023Quote: ... Each chromatin sample containing about 6,000 cells was incubated with 1 µg of anti-H3K27me3 antibody (Diagenode, C15410195, lot. A1811-001P). ChIP was performed using the automated platform SX-8G IP-Star platform (Diagenode ...
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bioRxiv - Molecular Biology 2024Quote: ... IP was performed overnight at 4° C using 5 μg antibody (anti-H3K9me3 Diagenode, ref. C15410056; anti-HA.11 BioLegend ref: 901503; anti-H3K4me3 Cell Signaling, ref. 9751S; anti-H3K36me3 Diagenode, ref ...
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bioRxiv - Molecular Biology 2019Quote: ... The lysed cells were sonicated to get an average size of 200-500 bp chromatin fragments using a Bioruptor (Diagenode, Seraing, Belgium). Chromatin was immunoprecipitated with 4 µg antibody against ChREBP (NB400-135 ...
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bioRxiv - Microbiology 2021Quote: 60,000 iATII cells or Calu-3 cells were incubated for 1 h at 4°C with equal protein concentrations of control rabbit IgG (Diagenode, Cat#C15410206) or 1/200 dilution of rabbit anti-ACE2 (Abcam ...
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bioRxiv - Genetics 2021Quote: ChlPseq was performed on 20k FACS sorted MM cells as previously described 44 using H3K27Ac antibodies (Diagenode Cat #C15410196, lot A1723-0041D/2) but with minor modifications on the preparation of input controls ...