Labshake search
Citations for Diagenode :
301 - 349 of 349 citations for Rat Integrin Alpha 2 ITGa2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pathology 2022Quote: ... library was prepared using the EXP-NBD103 and SQK-LSK108 kits according to the manufacturer’s instructions and starting with 3 μg of 20 kb sheared DNA (Megaruptor, Diagenode) as input ...
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bioRxiv - Microbiology 2022Quote: ... July 2020 release using the SMRTbell Express Template Prep Kit v2.0 with the following modifications: genomic DNA was sheared using Speed 34 on the Megaruptor 3 (Diagenode) and an additional size selection step of 6-50kb fragments on the Blue Pippin (Sage Science ...
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bioRxiv - Plant Biology 2023Quote: ... rRNA depleted RPFs were directly used as input for the D-plex small RNA-seq kit (Diagenode cat#C05030001), according to manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... 25 ng of RNA in 8 µL water was input to the D-Plex Small RNA kit (Diagenode, C0503000) and libraries were prepared according to the manufacturer recommendations with the exception that 9 cycles of PCR were carried out ...
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bioRxiv - Microbiology 2019Quote: ... pneumophila in the samples was detected by diagnostic PCR using primers and probes of the R-DiaLegTM kit (Diagenode, Belgium). The PCRs were prepared in a final volume of 20 μL by adding the Master Mix 5X (TaqMan Probe LC2.0 ...
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bioRxiv - Developmental Biology 2020Quote: ... the bisulfite conversion ratio was calculated using spike-in controls provided by Premium Reduced Representation Bisulfite Sequencing (RRBS) Kit (Diagenode) (supplementary Table S2 ...
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bioRxiv - Developmental Biology 2020Quote: ... samples were sent to the Genomics Unit of the Centre for Genomic Regulation (PRBB, Barcelona) where RRBS libraries were prepared using the Premium Reduced Representation Bisulfite Sequencing (RRBS) Kit (Diagenode), and sequenced ton an Illumina HiSeq 2000 platform in 50-bp single-end mode ...
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bioRxiv - Genetics 2020Quote: ... 50ng of immunoprecipitated DNA or input sample were used to prepare sequencing libraries using the iDeal Library Preparation kit (Diagenode), according to manufacturer instructions ...
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bioRxiv - Genetics 2019Quote: 0.5 × 106 frozen cells were resuspended in 250 µl buffer iL1 with proteinase inhibitors cocktail (iDeal ChIP-seq Kit for Histones, Diagenode) and incubated for 10 min at 4ºC on the Bohemian wheel ...
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bioRxiv - Genomics 2019Quote: ... Chromatin from hMSCs and differentiated chondrocytes were extracted using the Diagenode iDeal histone ChIP-seq kit (Diagenode SA, Ougrée, Belgium). Extracted chromatin was sonicated using a Diagenode Bioruptor Standard or Bioruptor Pico to an average size of 200-500bp ...
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bioRxiv - Cancer Biology 2022Quote: Tumour samples with optimal chromatin fragment size (200-500 bp) were immunoprecipitated using the iDeal ChIP-seq Kit for Histones (Diagenode). Magnetic immunoprecipitation of sheared DNA-chromatin complexes (500ng ...
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bioRxiv - Developmental Biology 2022Quote: ... in VFG media for 10 minutes at room temperature followed by a 5-minute quench with glycine (in True MicroChIP kit, Diagenode) at room temperature ...
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bioRxiv - Molecular Biology 2019Quote: ... Approximately 500 ng of total RNA was used for NGS libraries preparation with CATS mRNA-seq kit (Diagenode, Liege, Belgium) according to the manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2019Quote: ... Then DNA was recovered by AMPure XP Beads and used for methylated DNA immunoprecipitation (MeDIP) using the Magnetic Methylated DNA Immunoprecipitation Kit (Diagenode) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2019Quote: ... Libraries for reduced representation bisulfite sequencing (RRBS) were prepared from 100 ng DNA per sample using the Premium RRBS Kit (Diagenode). Final libraries were purified twice using Agencourt AMPure XP beads (Beckman Coulter ...
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bioRxiv - Cancer Biology 2021Quote: H3K27ac chromatin immunoprecipitation (ChIP) experiments were performed as previously described12 using the iDeal ChIP-seq kit for histones (Cat# C01010171, Diagenode) and the H3K27ac rabbit polyclonal antibody (Abcam Cat# ab4729 ...
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bioRxiv - Immunology 2021Quote: ... Chromatin was prepared from 40–50 million cells using Diagenode Chromatin shearing optimization kit (Cat no. C01010055) and further subjected to sonication using Bioruptor sonicator (Diagenode) to obtain chromatin fragments of 100–500 bps ...
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bioRxiv - Genomics 2021Quote: ... ChIP-seq for H3K4me3 and H3K27ac in PEER cell line was generated using MicroPlex Library Preparation Kit (Diagenode, Liege, Belgium) according to the BLUEPRINT protocol (http://dcc.blueprint-epigenome.eu/#/md/methods) ...
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bioRxiv - Genetics 2020Quote: ... 500 ng of each sample was converted into a sequencing-ready library using the CATS RNA-seq kit with rRNA depletion (Diagenode). The RNA integrity ...
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bioRxiv - Molecular Biology 2019Quote: ... or 2 ng (other antibodies) of ChIP-ed DNA was used to prepare DNA libraries with MicroPlex Library Preparation Kit v2 (Diagenode).
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bioRxiv - Plant Biology 2022Quote: ... 250 pg of immunoprecipitated (IP) or genomic (INPUT) DNA were used to prepare libraries with the MicroPlex Library Preparation kit v2 (Diagenode). Quality of libraries was validated using Agilent / Advanced Analytical fragment analyzer ...
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bioRxiv - Microbiology 2023Quote: ... chromatin-antibody complexes were washed and eluted from the beads using the iDeal ChIP-seq kit for transcription factors (Diagenode) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... DNA was purified as for ChIP-seq samples following the Diagenode iPure protocol using reagents from the Auto iDeal ChIP-seq kit (Diagenode). DNA quantification ...
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bioRxiv - Cell Biology 2023Quote: HUVEC and HUAEC cells that were subjected to ChIP were transduced with lentiviral vectors for 72-96 h before being processed using iDeal ChIP-seq kit for Transcription factors (Diagenode), according to the manufacturers protocol with modifications ...
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bioRxiv - Neuroscience 2023Quote: ... and 8 μl was used for small RNA libraries construction by the D-Plex Small RNA-seq Kit (Diagenode C05030001). Indexes were attached using the D-Plex Single Indexes for Illumina - Set A (Diagenode C05030010 ...
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bioRxiv - Genomics 2023Quote: ... The immunoprecipitated DNA and 1% of the total input were reverse-crosslinked and eluted using the iPure v2 kit (Diagenode). For library preparation ...
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bioRxiv - Molecular Biology 2023Quote: Libraries were then prepared from 10 ng of RPFs using the D-Plex Small RNA-Seq kit for Illumina (Diagenode) and the D-Plex Unique Dual Indexes for Illumina – set A (Diagenode) ...
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bioRxiv - Cancer Biology 2023Quote: The ChIP assay was carried out according to the protocol from the iDeal ChIP-Seq Kit for Transcription Factors (Diagenode). Briefly ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Several libraries were constructed using the Ligation Sequencing Kit (Nanopore LSK109) using DNA sheared to different size using a megaRuptor (Diagenode) to optimise yield and contiguity ...
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bioRxiv - Neuroscience 2021Quote: ... Chromatin immunoprecipitation was then performed on the sheared DNA using the iDeal ChIP-seq kit for transcription factors (Diagenode Cat# C01010170). One percent of chromatin was kept aside to be used as an input control ...
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bioRxiv - Molecular Biology 2019Quote: ... Approximately 10 ng of bisulfite-treated single-stranded DNA was directly used for NGS libraries preparation with CATS DNA-seq Kit v1.0 (Diagenode, Liege, Belgium) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... using the Capture and Amplification by Tailing and Switching method (CATS RNA-seq Kit v2 x24, Diagenode Cat# C05010041, Leige, Belgium). 10ng RNA was used as input and the dephosphorylation step omitted to select for 3’-OH RNA species (miRNA) ...
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bioRxiv - Plant Biology 2022Quote: ... The crosslinking was performed by vacuum infiltration (~ –950 millibars) for 20 min and ChIP-qPCR were performed using Diagenode’s Universal Plant kit (Diagenode, Seraing, Belgium). The shared chromatin was immunoprecipitated using anti-GFP antibody (Abcam 290) ...
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bioRxiv - Neuroscience 2023Quote: ... and 20 ng of BH RNAs were used for small RNA library construction by the D-Plex Small RNA-seq Kit (Diagenode C05030001). D-Plex Single Indexes for Illumina - Set A (DiagenodeC05030010 ...
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bioRxiv - Plant Biology 2022Quote: ... A HiFi library was then prepared according to the “Procedure & Checklist - Preparing HiFi SMRTbell® Libraries using SMRTbell Express Template Prep Kit 2.0” manual with an initial DNA fragmentation by Megaruptor 3 (Diagenode, Belgium) and final library size binning into defined fractions by SageELF (Sage Science ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were washed once with PBS before adding cold lysis buffer iL1b (from the Auto iDeal ChIP-seq Kit for Transcription Factors, Diagenode C01010058). Asynchronously growing cells (collected by scraping ...
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bioRxiv - Neuroscience 2023Quote: ... Libraries were constructed from 500 pg total RNA using D-Plex Small RNA-seq Kit for Illumina and Single Indexes for Illumina - Set B (Diagenode, C05030001). RIN aXer cell sorting was determined to be between 1.2 and 7.2 (Bioanalyzer 6000 ...
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Regulation of transcription patterns, poly-ADP-ribose, and RNA-DNA hybrids by the ATM protein kinasebioRxiv - Molecular Biology 2023Quote: ... 50mg patient tissue RNA was extracted in an RNase free environment using RNA Extraction kit for Bioruptor Plus (Diagenode Cat# C20000010). The additional protocol listed for DNaseI treatment was followed for 10ug of extracted RNA per sample ...
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bioRxiv - Cell Biology 2023Quote: ... a total of 10 ng of dsDNA was purified using the MicroPlex Library Preparation kit v3 (C05010001, Diagenode s.a., Seraing, Belgium), according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: Nucleosome assembly in the presence of Nap1 and ACF was carried out using a Chromatin assembly kit (C01030001) (Diagenode Inc. United States), following the manufacturer’s protocol with minor modifications to accommodate tracer DNA ...
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bioRxiv - Genomics 2022Quote: ... RRBS libraries were prepared for 23 samples with 200 ng genomic DNA according to the protocol described previously (Diagenode Premium RRBS kit) and kept at -80°C until submission to the GGBC for sequencing ...
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bioRxiv - Developmental Biology 2022Quote: ... were then incubated overnight at 4°C using primary antibodies for H3K27ac and SET in iC buffer from iDeal ChIP-seq kit for Histones (Diagenode, cod. C01010051). The next day ...
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bioRxiv - Molecular Biology 2024Quote: ... ChIP (Chromatin Immunoprecipitation) was performed using the reagents and protocol provided in the universal plant ChIP-Seq kit (Cat. No. C01010152; Diagenode, Ougree, Belgium) as per the manufacturer’s instructions ...
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bioRxiv - Genomics 2022Quote: ... libraries were constructed with 200 ng input DNA from each sample (n = 24; Table 1) using the Premium RRBS Kit (Diagenode, Cat. No. C02030032) according to manufacturer instructions ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: A total of 100 ng of total genomic liver DNA was diluted and processed using the Premium Reduced Representation Bisulfite Sequencing (RRBS) Kit (Diagenode, Denville, NJ, USA) as per the manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... ChIPseq libraries were prepared from 10 ng of double-stranded purified DNA using the MicroPlex Library Preparation kit v2 (C05010014, Diagenode s.a., Seraing, Belgium), according to manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: A total of 100ng of total DNA was diluted and processed using the Premium Reduced Representation Bisulfite Sequencing (RRBS) Kit (Diagenode, Denville, NJ, USA) as per the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... 200,000 cells were centrifuged at 500g before proceeding with the nuclei isolation and transposition reaction using the ATAC-seq Kit from Diagenode (Diagenode Cat #C01080002). According to the results of the nuclei isolation optimization ...
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bioRxiv - Evolutionary Biology 2023Quote: ... High molecular weight DNA was extracted using a Circulomics Nanobind Tissue Big DNA Kit (NB-900-701-001) and sized to 15-25 kb using Bioruptor (Diagenode, Denville, NJ, USA). Sequencing libraries were constructed according to the manufacturers’ protocols and each sample was sequenced on one PacBio Sequel II 8M SMRT Cell for 30 hours in circular consensus sequencing mode to generate about 20 Gb of HiFi sequence data ...