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Citations for Diagenode :
1 - 50 of 417 citations for Rat High Mobility Group Protein B1 HMGB1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2021Quote: ... anti-GT1 (1:75) and anti-YSPTSPS repeat S2Pho (RNA Pol II, B1 subunit; 1:200, Diagenode). The samples were washed for 8h at 4°C with gentle agitation with PBS (0.2% v/v Tween-20 ...
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Increased ACTL6A Occupancy Within mSWI/SNF Chromatin Remodelers Drives Human Squamous Cell CarcinomabioRxiv - Molecular Biology 2021Quote: ... high output (Diagenode Bioruptor). After centrifugation at 15,000 rpm for 10 min to collect the supernatants ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were lysed and chromatin was prepared using the Chromatin EasyShear Kit - High SDS (Diagenode; Cat# C01020012), and shearing of the chromatin was achieved using the Bioruptor with four rounds of ten cycles of 30 seconds “ON„ and 30 seconds “OFF„ at HIGH power setting (position H) ...
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bioRxiv - Molecular Biology 2021Quote: ... The Low Cell# ChIP kit protein A × 48 was from Diagenode (Denville, NJ, USA) and the Chemiluminescence western blot detection reagent was from Thermo Fisher Scientific (Waltham ...
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bioRxiv - Biochemistry 2020Quote: ... at HIGH setting (Bioruptor Plus, Diagenode). Samples were centrifuged 10 min at 12,000 × g and 10% of the sample was saved as input DNA and stored at −80 °C until reversal of cross-linking ...
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bioRxiv - Cell Biology 2020Quote: ChIP-seq experiments were carried out using the HighCell# ChIP-Protein G kit (Diagenode, Cat#: C01010063) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... incubated in blocking buffer with 1:500 rat anti-5hmC (Diagenode) overnight at 4°C and followed by incubation with 1:1000 anti-rat IgG HRP (ab6734 ...
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bioRxiv - Microbiology 2021Quote: ... 30s OFF (setting high, BioruptorTM Next Gen, Diagenode) (Fraschka et al. ...
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bioRxiv - Microbiology 2020Quote: ... high power at 4°C (Bioruptor Plus, Diagenode)] ...
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bioRxiv - Systems Biology 2022Quote: ... before high-energy sonication to shear DNA (Diagenode Bioruptor pico). The protein concentration was determined through a BCA assay (Thermo Fischer) ...
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bioRxiv - Plant Biology 2021Quote: ... High-molecular-weight DNA was sheared with a Megaruptor (Diagenode) to obtain 15-20 kb fragments ...
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bioRxiv - Genomics 2021Quote: ... The cells were lysed by high energy sonication (Bioruptor, Diagenode) and reduced by adding DTT (to a final concentration of 15 mM and heating for 30 minutes at 56 °C) ...
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bioRxiv - Genetics 2021Quote: ... lysed at high performance with a Bioruptor Plus sonicator (Diagenode) using 10 cycles of 30 s sonication intervals ...
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bioRxiv - Plant Biology 2022Quote: ... high molecular weight DNA was sheared with a Megaruptor (Diagenode) set to a 40 kb fragment size ...
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bioRxiv - Genetics 2021Quote: ... Lysates were then sonicated at high intensity in a Bioruptor (Diagenode) water bath to shear DNA to desired size range (300-500 bp ...
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bioRxiv - Cancer Biology 2022Quote: ... Lysates were sonicated at high power with a Bioruptor sonicator (Diagenode) for 15 min ...
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bioRxiv - Microbiology 2023Quote: High molecular weight gDNA was sheared with the Megaruptor 3 (Diagenode) to obtain 10- 15 kbp fragments ...
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bioRxiv - Plant Biology 2023Quote: ... and sonicated four times (BioruptorTM, Diagenode, 30s on/1min off on High). They were quantified at 205 nm using the Nanodrop 2000 according to the manufacturer’s instruction (Thermoscientific ...
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bioRxiv - Cancer Biology 2024Quote: ... Nuclei were then sonicated at high energy in a Bioruptor Plus (Diagenode). Sonication parameters to produce DNA fragments in the 200-600 bp range were optimized beforehand ...
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bioRxiv - Genomics 2024Quote: ... High molecular weight genomic DNA was sheared mechanically on Megaruptor 3 (Diagenode) to generate 30 Kb fragments ...
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bioRxiv - Genomics 2021Quote: ... Dynal Protein A magnetic beads (Diagenode) and ChIP-graded anti-myc antibody (ab9132 ...
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bioRxiv - Biochemistry 2023Quote: ... Unblocked protein A beads (Diagenode, C03020003) were used to pulldown the antibody-protein complexes ...
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bioRxiv - Cancer Biology 2021Quote: ... The DNA-protein-antibody complexes were captured by DiaMag protein A-coated magnetic beads (Diagenode; Cat#: C03010020) by incubating at 4 °C for 2 hours ...
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bioRxiv - Cancer Biology 2021Quote: ... The DNA-protein-antibody complexes were captured by DiaMag protein A-coated magnetic beads (Diagenode; Cat#: C03010020) by incubating at 4 °C for 2 hours ...
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bioRxiv - Systems Biology 2019Quote: ... Samples were further sonicated (15 cycles at high intensity using a Bioruptor, Diagenode) and boiled (90°C ...
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bioRxiv - Cell Biology 2022Quote: ... with 30 sec breaks on high-performance mode with Bioruptor Plus (#B01020001, Diagenode). Samples were centrifuged at 20000 x g for 20 min at RT ...
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bioRxiv - Neuroscience 2023Quote: ... and protein extraction beads (Diagenode, NJ, USA) according to the manufacturer instructions ...
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bioRxiv - Cancer Biology 2019Quote: ... Twenty cycles of sonication (30s high, 30s off) using TPX tube (Diagenode, ref: 50001) and the Bioruptor (Diagenode ...
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bioRxiv - Cancer Biology 2021Quote: ... and then sonicated for 5 min at high intermittent pulses (30/30) (Diagenode, Bioruptor). Samples were centrifuged for 10 min at 10,000 g at 4°C to remove any cell debris and typically 25 μg proteins were separated on SDS-polyacrylamide gels and analysed by Western blotting ...
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bioRxiv - Genomics 2023Quote: ... in the high mode at 4°C in a Bioruptor water bath sonicator (Diagenode), to obtain fragments of 500 to 1000 bp in size ...
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bioRxiv - Neuroscience 2022Quote: ... Chromatin-protein complexes were immunoprecipitated with protein A/G magnetic beads and washed sequentially according to the manufacturer (Diagenode, C01010055). DNA fragments were then purified using IPure beads v2 (Diagenode ...
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bioRxiv - Genomics 2022Quote: ... Each high-quality gDNA (200 ng) was fragmented by sonication using a Bioruptor (Fa. Diagenode) and subsequently used for library preparation with the TruSeq Nano DNA library preparation kit (Fa ...
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bioRxiv - Cancer Biology 2021Quote: ... sonicated 5 minutes (Bioruptor plus, Diagenode, 5 cycles high, 30 s on, 30 s off) and incubated at 37 °C for 60 min ...
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bioRxiv - Cell Biology 2020Quote: ... sonicated (5 cycles of 30 s ON, 30 s OFF, high power, Bioruptor Plus, Diagenode), spun for 10 min at 16,000 × gmax to remove any insoluble material ...
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bioRxiv - Molecular Biology 2019Quote: ... Lysates were sonicated at medium-high intensity for 10 minutes in a Bioruptor Standard (Diagenode) placed at 4°C and subsequently boiled for 10 minutes at 90°C ...
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bioRxiv - Genomics 2023Quote: ... and then high-quality genomic DNA was randomly fragmented using a Megaruptor (Diagenode, NJ, USA). High molecular weight (HMW ...
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bioRxiv - Cell Biology 2023Quote: ... Cell lysates were sonicated for 15 min at high power with the Bioruptor sonicator (Diagenode). Protein quantification was performed using the Bradford reagent (Biorad #5000001) ...
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bioRxiv - Developmental Biology 2019Quote: ... overnight and Protein A magnetic beads (Diagenode C03010020) the following day for 2 hours ...
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bioRxiv - Cancer Biology 2022Quote: ... homogenization was performed using protein extraction beads (Diagenode) and sonication in the lysis buffer followed by centrifugation to remove solid remains (Diagenode Bioruptor Plus sonicator manufacturer’s protocol for protein extraction from tissue fragments) ...
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bioRxiv - Developmental Biology 2019Quote: ... ChIP was performed using Protein G beads (Diagenode) O/N using a H3K27ac antibody (Active Motif ...
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bioRxiv - Microbiology 2023Quote: ... magnetic beads coated with DiaMag protein A (Diagenode), and antibodies against epitope tags (HA or MYC ...
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bioRxiv - Neuroscience 2023Quote: ... RNA samples were incubated overnight at 4°C with 12.5 µg of anti-5-hmC antibody (Diagenode rat monoclonal MAb-633HMC) or without antibody as negative control in IP buffer (750 mM NaCl ...
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bioRxiv - Genomics 2020Quote: ... followed by sonication at high power for 10 cycles of 30s ON/OFF (Bioruptor Plus; Diagenode). Lysates were clarified by centrifugation at 10,000g for 15min ...
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bioRxiv - Genomics 2019Quote: ... 20 µg high molecular weight genomic DNA was sheared using the Megaruptor instrument (Diagenode, Liege, Belgium) to 40kb and this was used as input into the SMRTbell library preparation ...
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bioRxiv - Cell Biology 2022Quote: ... The lysates were sonicated (30 s/30 s, 10 cycles, high performance) by Bioruptor (Diagenode; B01020001), followed by centrifugation at 21000xg for 20 min at 20°C ...
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bioRxiv - Molecular Biology 2023Quote: ... for 20 cycles (30 sec on/30 sec off, high-intensity) in a Bioruptor NextGen (Diagenode). Chromatin and DNA samples were processed in DNA LoBind tubes from here on (Eppendorf #2023-04-28 ...
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bioRxiv - Plant Biology 2021Quote: ... Protein extract was sonicated using a cooled Biorupter (Diagenode) for 10 minutes using high power with 30 seconds on 30 seconds off cycle ...
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bioRxiv - Plant Biology 2020Quote: ... magnetic beads (DiaMag protein A-coated magnetic beads: Diagenode) were incubated with each antibody overnight ...
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bioRxiv - Molecular Biology 2023Quote: ... Total proteins were sonicated on a Bioruptor Plus (Diagenode) (position high ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein extracts were finally sonicated in a Bioruptor (Diagenode) for 3 cycles at high intensity ...