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Citations for Diagenode :
301 - 350 of 406 citations for Rabbit IgG Anti SARS CoV 2 Spike S1 Antibody CR3022 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... or anti-PolII (Diagenode C15200004 and sc-55492) antibody was added and incubated overnight at 4°C on a rotor ...
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bioRxiv - Systems Biology 2020Quote: ... Chromatin was broken into small pieces with an average size of 0.5-2 kb using the Bioruptor (Diagenode). The sonicated chromatin was then incubated with 5 µg of primary antibodies overnight at 4°C ...
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bioRxiv - Evolutionary Biology 2019Quote: HMW genomic DNA was sheared to 30 kb using a Megaruptor® 2 instrument (Diagenode SA, Seraing, Belgium). DNA-damage and end repair was carried out prior to blunt adaptor ligation and exonuclease purification using ExoIII and ExoVII ...
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bioRxiv - Cell Biology 2019Quote: ... Immunoprecipitation was performed by incubating 2µg of H3K4me3 antibody (Diagenode pAb-030-050 - C15410030) or IgG (sc-2025 ...
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bioRxiv - Molecular Biology 2020Quote: ... followed by immunostaining with Cas9 monoclonal antibody 4G10 (Diagenode cat#: C15200216; 1:200 dilution) with gentle shaking for 2h at room temperature ...
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bioRxiv - Molecular Biology 2019Quote: ... The following antibodies were used for western blot analysis: Cas9 (Diagenode C15310258, 1:1000) TCF4 (Abcam ab217668 ...
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bioRxiv - Microbiology 2021Quote: ... Antibodies against MBD1 (pAb-078-050) and UHRF1 (H00029128-B01P) were purchased from Diagenode and Abnova ...
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bioRxiv - Genetics 2020Quote: ... Mouse chromatin was ChIPped using the following amounts of antibody: 1µg H3K4me3 (Diagenode C1540003), 0.2µg H3K4me1 (Diagenode C1540194) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Samples were then incubated for 14 hours with the following antibodies: H3K27ac (Diagenode, C15410196), H3K27me3 (abcam ...
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bioRxiv - Developmental Biology 2024Quote: ... The supernatant was transferred to a fresh tube containing H3K4me3 antibody (Diagenode Catalog# 15410003) bound protein G Dynabeads that were washed and resuspended in 100 µL of combined buffer ...
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bioRxiv - Immunology 2021Quote: ... followed by the addition of either anti-H3K9me3 (Diagenode) or control rabbit IgG (Diagenode ...
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bioRxiv - Cell Biology 2020Quote: ... as follows: anti-H3pan (1 μl/ChIP, C15310135 Diagenode), anti-H3K9me3 (2 μl/ChIP ...
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bioRxiv - Biochemistry 2021Quote: Cellular human and viral material in sputum deposited on glass slides was lysed in 50 μl 2% SDS dissolved in 50 mM Tris/HCl (pH 8.2) followed by sonication in a Bioruptor Pico (Diagenode). Proteins were digested using the SP3 protocol as described below.
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bioRxiv - Cell Biology 2021Quote: ... DNA was fragmented with 2 rounds of 30 cycles 30sec ON-30sec OFF of sonication with Bioruptor Plus (Diagenode) set on HIGH and purified with Qiagen MinElute kit (Qiagen) ...
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bioRxiv - Microbiology 2022Quote: ... The DNA was sheared to a modal size of 10 kb using a Megaruptor 2 (Diagenode Diagnostics, Seraing (Ougrée), Belgium) ...
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bioRxiv - Molecular Biology 2023Quote: ... All samples were lysed the same day 2 weeks before proteomics analysis in 100µL iST-NHS-Lysis buffer (PreOmics GmbH) by sonication (Bioruptor (DIagenode) 15s ON/15s OFF for 15 min) ...
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bioRxiv - Molecular Biology 2021Quote: ... with following appropriate antibodies at 4 °C o/n a rotating wheel: H3K27me3 (Diagenode, C15410195), RING1B (Cell Signaling ...
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bioRxiv - Genetics 2022Quote: ... Immunoprecipitation was performed using 10 μg of chromatin and 2.5 μg of human antibody (H3K4me3 [RRID:AB_2616052, Diagenode C15410003], H3K4me1 [RRID:AB_306847, Abcam ab8895], and H3K27ac [RRID:AB_2637079, Diagenode C15410196]). Final DNA purification was performed using the GeneJET PCR Purification Kit (Thermo Scientific ...
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bioRxiv - Systems Biology 2023Quote: ... ∼400 ng DNA-containing chromatin was incubated with 1 µg antibody (H3K9/14ac (Diagenode, C15410005); H3K9me2 (in house ...
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bioRxiv - Genomics 2023Quote: ... The sample was then incubated with antibodies (AR: Abcam ab133273, FOXA1: Abcam ab23738, H3K27ac: Diagenode 3C15410196 ...
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bioRxiv - Immunology 2021Quote: ... Samples were split into 2 x 1.5 mL in 15 mL polypropylene tubes suitable for the Bioruptor® Pico (Diagenode). BioRuptor Sonicator settings ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were lysed and sonicated in the presence of 0.2% SDS for 30 cycles (30 s on, 30 s off) using the high setting on a Bioruptor sonicator (Diagenode). Cell debris were pelleted by centrifugation and SDS in the lysate supernatants was diluted to 0.07% ...
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bioRxiv - Genomics 2020Quote: ... Nuclei were lysed for 15 min on ice with occasional inversion every 2 min and then sonicated for 24 cycles with 30s on and 30s off using Bioruptor (Diagenode). After sonication ...
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bioRxiv - Molecular Biology 2020Quote: ... nucleic acids gently extracted from unfixed NTERA-2 cells were sheared by sonication (12 cycles at high power, 15s ON / 90s OFF) with a Bioruptor (Diagenode). 4.4 μg of sheared nucleic acids were incubated with 10 μg of S9.6 antibody for immunoprecipitation overnight at 4°C ...
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bioRxiv - Developmental Biology 2020Quote: ... Sonication was performed by splitting each sample to 2 Bioruptor tubes with sonication beads and using the Bioruptor Pico (Diagenode) for 18 cycles of 30 sec on and 30 sec off into an average size of approximately 200 bp ...
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bioRxiv - Microbiology 2020Quote: ... The supernatant was transferred to a sterile 2 ml microcentrifuge tube and sonicated with the Bioruptor UCD-200 sonicator (Diagenode) for 1 min on the low setting (160W at 20kHz ...
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bioRxiv - Neuroscience 2020Quote: ... the crosslinked cells were resuspended in 2 mL of lysis buffer and sonicated (in a cold room) by using a Bioruptor (Diagenode) with the following parameters ...
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bioRxiv - Genomics 2020Quote: ... high molecular weight DNA was diluted to a concentration of 25 ng/uL and fragmented to an average peak size of 25 kb using a Megaruptor-2 instrument (Diagenode). Small fragments <1 kb were removed and the DNA concentrated using bead purification (0.4 x volumes of Ampure-XP beads ...
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bioRxiv - Biochemistry 2022Quote: ... incubated at 95°C for 5 min and sonicated at 4°C for 2 cycles of 20 s at high intensity level using a BioRuptor (Diagenode). Samples were clarified by centrifugation at 16,000 x g for 15 min ...
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bioRxiv - Developmental Biology 2019Quote: ... and sonication for 2 cycles of 15s ON and 15s OFF on medium power at 4°C on a PicoBioruptor (Diagenode). After 10 minutes incubation at 4°C ...
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bioRxiv - Developmental Biology 2019Quote: ... and sonicated for 2 cycles of 15s ON and 15s OFF on full power at 4°C on a Bioruptor (Diagenode). Samples were incubated for 1 hour on a rotating wheel at 4°C and centrifuged 10 minutes at 12,000 g and 4°C ...
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bioRxiv - Molecular Biology 2022Quote: ... DNA was purified using phenol/chloroform and 2 µg of purified 3C sample was sonicated using the Bioruptor Pico (Diagenode). Biotinylated DNA was captured using MyOne Streptavidin T1 magnetic beads (Life Technologies ...
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bioRxiv - Neuroscience 2023Quote: ... α-Synuclein PFFs were vortexed and diluted with DPBS to 2 mg/mL and sonicated in a cooled bath sonicator at 9°C (Diagenode Bioruptor® Pico ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were lysed on ice for 10 minutes prior to sonication for either 20 cycles (replicate 1) or 40 cycles (replicate 2) of 30s on / 30s off with a Bioruptor UCD-200 (Diagenode) at maximum power ...
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Heat Shock Factor 1 (HSF1) as a new tethering factor for ESR1 supporting its action in breast cancerbioRxiv - Cancer Biology 2021Quote: ... and mouse anti-ERalpha (1:200; # C15100066, Diagenode, Liège, Belgium); negative controls were proceeded without one primary antibody or both ...
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bioRxiv - Genomics 2021Quote: ... Mouse anti-Cas9 (Encor MCA-3F9 and Diagenode C15200229-100) antibodies were used ...
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bioRxiv - Cancer Biology 2019Quote: ... ChIP-seq was performed using the protocol previously described (51) with antibodies to H3K27Ac (C15410196, Diagenode) and H3K27me3 (9733S ...
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bioRxiv - Physiology 2021Quote: ... Immunoprecipitation was performed using 10 μg of chromatin and 2.5 μg of antibody (H3K4me3 (Diagenode C15410003), H3K4me1 (abcam ab8895 and H3K27ac (Diagenode C15410196) ...
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bioRxiv - Molecular Biology 2022Quote: ... chromatin of each replicate was incubated with 1 μg of antibody against H3K27ac (Diagenode, C15410196, RRID:AB_2637079) or 1 μg of antibody against ONECUT2 (Proteintech ...
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bioRxiv - Genomics 2022Quote: ... Proximity ligated chromatin pellet was sonicated and immunoprecipitated using 5 μg of H3K27ac antibodies (Diagenode, C15410196). Immunoprecipitated fragments were Biotin labeled (Invitrogen ...
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bioRxiv - Systems Biology 2023Quote: ... single-stranded DNA mixture was incubated with 5-mC antibody provided by MagMeDIP-seq Package (Diagenode), followed by 14-16 cycles of library amplification ...
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bioRxiv - Genomics 2023Quote: ... respectively) of the primary antibodies (C15410003 and C15410196 for H3K4me3 and H3K27ac, respectively; Diagenode, Denville, NJ), 0.05% (w/v ...
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bioRxiv - Immunology 2024Quote: ... Beads were blocked with 1 µL of antibody (α-H3K27ac, Diagenode, C15410196; α-H3K4me3, Diagenode, C15410003) in antibody binding buffer for 3-4h at 4°C with rotation ...
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bioRxiv - Cancer Biology 2024Quote: ... 25µg of sheared chromatin was then incubated with 4µg of antibody against H3K4me3 (Diagenode, C15410003-50) H3K36me3 (Abcam ...
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bioRxiv - Microbiology 2020Quote: ... was added to the lysate and incubated at 37 °C for 10 min before sonication (2 × 8 bursts of 30 sec on ice using a Diagenode Bioruptor) to shear DNA fragments to a length of 300 to 500 bp ...
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bioRxiv - Microbiology 2022Quote: ... were added to the lysate and incubated at 37 °C for 10 min before sonication (2 x 8 bursts of 30 sec on ice using a Diagenode Bioruptor) to shear DNA fragments to a length of 300 to 500 bp ...
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bioRxiv - Biochemistry 2022Quote: ... heated to 95°C for 10 min shaking (1000 rpm) followed by 2 cycles à 20 s of sonication in BioRuptor (Diagenode). Aliquots containing 50 μg protein were transferred into a cartridge and cooled ...
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bioRxiv - Genomics 2022Quote: ... HMW-DNA (25 ng/μl) was separately sheared with 30 kb and 35 kb settings using a Megaruptor 2 instrument (Diagenode SA). However ...
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bioRxiv - Molecular Biology 2019Quote: ... 2% Triton-100 with protease inhibitors) and sonicated using a Bioruptor Pico (Diagenode, 3× cycles 30 sec on / 30 sec off). Sonicated lysates were diluted 2× in 50mM Tris ...
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bioRxiv - Molecular Biology 2019Quote: ... 2% Triton-100 with protease inhibitors and sonication using a Bioruptor Pico (Diagenode, 3× cycles 30 sec on / 30 sec off). 200μg of proteins for each sample were diluted to 1 ml with CoIP Lysis Buffer and incubated with 30μl of anti-FLAG M2 Magnetic Beads (Sigma M8823 ...