Labshake search
Citations for Diagenode :
51 - 100 of 144 citations for Phthalic Acid Unlabeled 100 Ug Ml In Mtbe since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Genomics 2019Quote: ... transferred to 15-ml Sumilon TPX tubes (Diagenode C30010009) and sonicated using a Diagenode Bioruptor 300 at 4°C and high power ...
-
bioRxiv - Developmental Biology 2020Quote: ... Worms were distributed into 15 ml TPX tubes (Diagenode) to reach 200–400 µl worm pellet per tube ...
-
bioRxiv - Molecular Biology 2022Quote: ... Bioruptor 0.5 ml Microtubes for DNA Shearing from Diagenode. Agencourt AMPure XP beads were purchased from Beckman Coulter ...
-
bioRxiv - Molecular Biology 2022Quote: ... Bioruptor 0.5 ml Microtubes for DNA Shearing from Diagenode. Agencourt AMPure XP beads were purchased from Beckman Coulter ...
-
bioRxiv - Molecular Biology 2023Quote: ... using 350 mg/mL of sonication beads (Diagenode, C03070001) for 40 cycles of 30 s ON ...
-
bioRxiv - Cancer Biology 2024Quote: ... and of antibodies 5 μg/ml IgG (Diagenode, C15410206), anti-CCL2 (Novus Biologicals ...
-
bioRxiv - Genetics 2020Quote: ... The purified gDNA was resuspended in 100 μl TE and sonicated using a BioRuptor Plus (Diagenode) with settings ...
-
bioRxiv - Bioengineering 2019Quote: ... genomic DNA was first fragmented to 100-500 bp by sonication using a Bioruptor Pico (Diagenode), with the following conditions ...
-
bioRxiv - Molecular Biology 2019Quote: ... and split over 2x 1.5 ml bioruptor pico microtubes (Diagenode). The cells were lysed and the chromatin was sheared in a bioruptor pico sonicator (Diagenode ...
-
bioRxiv - Genetics 2020Quote: ... Immunoprecipitation was performed using 75 µg of chromatin and 5 µg of Cas9 antibody (Diagenode #C15200229-100) or 15 µg of chromatin and 2.5 µg of H3K27ac and H3K9me3 antibody (Active Motif #3913 ...
-
bioRxiv - Genomics 2020Quote: ... and 1 EDTA-free protease inhibitor tablet (Roche)/10 mL in H2O) before sonication with a Bioruptor Pico (Diagenode) for 5 minutes (30 seconds on ...
-
bioRxiv - Synthetic Biology 2021Quote: ... The nuclei were sonicated utilizing 15 ml Bioruptor tubes (Diagenode, C01020031) with 437.5 mg sonication beads (Diagenode ...
-
bioRxiv - Cell Biology 2023Quote: ... TPCK 5 μg/mL) and sonicated on a Bioruptor Pico (Diagenode) for 15 cycles 30 sec ON/30 sec OFF in 1.5 mL Diagenode tubes (Diagenode ...
-
bioRxiv - Synthetic Biology 2023Quote: Bicone suspensions were dispensed into 1.5 mL polymethylpentene (TPX) tubes (Diagenode) and allowed to equilibrate overnight at ambient conditions ...
-
bioRxiv - Cancer Biology 2023Quote: ... TPCK 5 mg/mL) and sonicated on a Bioruptor Pico (Diagenode) for 15 cycles 30 sec ON/30 sec OFF in 1.5 mL Diagenode tubes (Diagenode ...
-
bioRxiv - Genomics 2023Quote: ... Chromatin was fragmented in 15 mL tubes using the bioruptor (Diagenode) for 12 cycles (30 sec on/30 sec off) ...
-
bioRxiv - Molecular Biology 2022Quote: ... and chromosomal DNA sheared to 100 to 500 bp using Bioruptor Plus water bath sonication system (Diagenode, Liège, Belgium). After centrifugation to remove cell debris ...
-
bioRxiv - Microbiology 2020Quote: ... 10-20 ng/μL of DNA extract in 100 µL chilled TLE buffer (10mM Tris, pH8.0, 0.1mM EDTA) was sheared using a Bioruptor™ (Diagenode) on high power for up to 15 min ...
-
bioRxiv - Cell Biology 2020Quote: Acetone-precipitated protein pellets were resuspended in 100 mM TEAB-buffered solution (pH 8.3) with the assistance of sonication (Bioruptor Plus, Diagenode, 5 cycles of 30 s ON ...
-
bioRxiv - Cancer Biology 2023Quote: ... Samples were taken up in 100 μL LYSE buffer and mixed with 50 mg of protein extraction beads (Diagenode). Samples were sonicated with 10-20 cycles at 30sec ON/OFF in a Diagenode Bioruptor Plus until complete tissue disruption was observed ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... The chromatin lysate was sonicated to a DNA fragment size range of 100-200bp using a Biorupter sonicator (Diagenode). CDK9 was immunoprecipitated with 15μg of an anti-CDK9 antibody (D-7 ...
-
bioRxiv - Neuroscience 2023Quote: ... 1:100) followed by sonication for 16 cycles (bursts and intervals of 30s each) with the Bioruptor® (Diagenode), which produced 200-1000 bp genomic DNA fragments ...
-
bioRxiv - Immunology 2022Quote: ... Cell lysate was transferred to 1.5 ml Bioruptor Plus TPX microtube (Diagenode) and was sonicated for 4 rounds of 15 cycles of 30 seconds on/30 seconds off on high power (Diagenode Biorupter plus) ...
-
bioRxiv - Molecular Biology 2021Quote: ... Chromatin was sheared by sonication in 15 ml Bioruptor tubes (Diagenode, C01020031) with 437.5 mg sonication beads (Diagenode ...
-
bioRxiv - Developmental Biology 2022Quote: ... and the lysate was sonicated in 0.65 ml Bioruptor Pico Microtubes (Diagenode). Chromatin was sheared using a Bioruptor Pico (Diagenode ...
-
bioRxiv - Genetics 2021Quote: Chromatin was transferred to a 15 ml conical tube (Diagenode, cat. # C30010017) for sonication in a Bioruptor Pico instrument (Diagenode ...
-
bioRxiv - Molecular Biology 2023Quote: ... Chromatin was sonicated in 1.5 mL TPX microtubes (#C30010010, Diagenode, Seraing, Belgium) for 20 cycles (30 sec on/30 sec off ...
-
bioRxiv - Molecular Biology 2022Quote: ... transferred to 1.5 mL sonication tubes (Diagenode C30010016, 300 µL per tube), and sonicated for five min total (10 cycles of 30 s on/off ...
-
bioRxiv - Biophysics 2024Quote: ... Chromatin was sheared by sonication in 15 ml Bioruptor tubes (Diagenode, C01020031) with 437.5 mg sonication beads (Diagenode ...
-
bioRxiv - Immunology 2020Quote: ... chromatin was sheared using a Bioruptor Pico Sonicator (5 cycles: 30 seconds ON - 30 seconds OFF, fragments 100 - 300 bp, Diagenode) and 10 % of sheared chromatin was kept as input control whilst 90 % was immunoprecipitated overnight using antibodies against H3K27ac ...
-
bioRxiv - Neuroscience 2019Quote: ... Libraries for reduced representation bisulfite sequencing (RRBS) were prepared from 100 ng DNA per sample using the Premium RRBS Kit (Diagenode). Final libraries were purified twice using Agencourt AMPure XP beads (Beckman Coulter ...
-
bioRxiv - Neuroscience 2023Quote: ... Overnight incubation with primary antibodies at 4°C was performed using the following antibodies: Cas9 (Diagenode, C15200230-100, 1:500), alpha synuclein (Abcam ...
-
bioRxiv - Molecular Biology 2023Quote: ... The eluate was transferred to a new tube containing 100 μl of TE buffer and then incubated with proteinase K (Diagenode) at 55 °C for 1 h ...
-
bioRxiv - Microbiology 2023Quote: ... and chromosomal DNA was sheared to 100-500 bp fragments using the Bioruptor Pluswater bath sonication system (Diagenode, Liège, Belgium). Lysates were incubated with 40 μL Anti-FLAG M2 affinity gel (cat A2220 ...
-
bioRxiv - Microbiology 2023Quote: ... The pellet corresponding to the nuclear fraction is dissolved in 300 µL of 1x SDS loading buffer with 100 mM DTT and sonicated with Bioruptor (Diagenode) 5 cycles of 30 seconds ON 30 seconds OFF intervals at high intensity ...
-
bioRxiv - Cell Biology 2020Quote: ... The DNA was then transferred to 1.5 ml TPX tubes (Diagenode, C30010010-1000) and sonicated for 4 rounds of 10 minutes (high power ...
-
bioRxiv - Genomics 2019Quote: ... The DNA was then transferred to 1.5 ml TPX tubes (Diagenode, C30010010-1000) and sonicated for 4 rounds of 10 minutes (high power ...
-
bioRxiv - Genomics 2022Quote: ... shearing was performed in 0.65 ml Bioruptor® Pico Microtubes (Diagenode Cat# C30010011). 25μl of this chromatin was used to assess the size of the DNA fragments obtained by High Sensitivity NGS Fragment Analysis Kit (DNF-474 ...
-
bioRxiv - Microbiology 2024Quote: ... by repeated slow-pipetting after transfer to 1.5 mL Bioruptor Micro tubes (Diagenode) on ice ...
-
bioRxiv - Molecular Biology 2019Quote: ... 2% Triton-100 with protease inhibitors) and sonicated using a Bioruptor Pico (Diagenode, 3× cycles 30 sec on / 30 sec off). Sonicated lysates were diluted 2× in 50mM Tris ...
-
bioRxiv - Molecular Biology 2019Quote: ... 2% Triton-100 with protease inhibitors and sonication using a Bioruptor Pico (Diagenode, 3× cycles 30 sec on / 30 sec off). 200μg of proteins for each sample were diluted to 1 ml with CoIP Lysis Buffer and incubated with 30μl of anti-FLAG M2 Magnetic Beads (Sigma M8823 ...
-
bioRxiv - Biochemistry 2021Quote: ... 10 mM EDTA and 100 mM NaCl) were added to the nuclei pellet and sonicated with the BioRuptor UCD-200 (Diagenode, Belgium) for 25 cycles at high power and 30 s intervals ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... DNA was diluted to 100 ng/uL (10,000 ng total) and sheared to approximately 500 bp using a BioRupter (Diagenode, Denville, NJ). We performed a double-sided size selection using SPRI beads (46 ...
-
bioRxiv - Microbiology 2023Quote: ... The DNA was diluted to 100 µL at a concentration of 10 ng / µL and then sheared using a Biorupter® Pico (Diagenode) with 13 cycles of 30 s on followed by 30 s off and quantified using an Agilent D1000 ScreenTape (Agilent Technologies) ...
-
Higher meiotic chromosome condensation: a potential function of kinetochore through polo-like kinasebioRxiv - Cell Biology 2023Quote: ... The chromatin was then sheared to 300-500 bp using a water bath sonicator (Diagenode SA, Picoruptor, BC 100, LAUDA Germany) for 30 cycles of 30 s ON / 30 s OFF at 4°C ...
-
bioRxiv - Molecular Biology 2024Quote: ... for a total volume of 100 μL and sonicated (4 cycles of 20sec/30sec (on/off time)) using a Bioruptor (Pico II, Diagenode, BE). The successful generation of fragments with a mean fragment size of 400 nucleotides was confirmed using a Bioanalyzer as per manufacturer’s instructions (Agilent ...
-
bioRxiv - Genomics 2021Quote: ... 0.5 % NP-40) and evenly split into pre-cooled 1.5 ml Bioruptor tubes (Diagenode). After sonication (15 sec on/ 30 sec off ...
-
bioRxiv - Immunology 2020Quote: 1.5 ml Bioruptor Microtubes were filled with 250 mg of Protein Extraction Beads (Diagenode) and filled with RIPA buffer (supplemented with protease and phosphatase inhibitors) ...
-
bioRxiv - Neuroscience 2019Quote: ... Nuclei diluted in 1 ml RIPA buffer were sonicated using Bioruptor (Diagenode, Denville, NJ) for 6 cycles of 5 minutes (60 seconds on/30 seconds off ...
-
bioRxiv - Neuroscience 2021Quote: ... 1mg/mL BSA) and sonicating at full strength for 15 min (Diagenode, Bioruptor Plus). Protein concentration was measured using a Bradford Assay and normalized so that all assay inputs contained the same amount of protein ...