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Citations for Diagenode :
151 - 200 of 201 citations for PEGSSDA 2 x 0.5 mL since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... DNA was purified using phenol/chloroform and 2 µg of purified 3C sample was sonicated using the Bioruptor Pico (Diagenode). Biotinylated DNA was captured using MyOne Streptavidin T1 magnetic beads (Life Technologies ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were lysed on ice for 10 minutes prior to sonication for either 20 cycles (replicate 1) or 40 cycles (replicate 2) of 30s on / 30s off with a Bioruptor UCD-200 (Diagenode) at maximum power ...
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bioRxiv - Cell Biology 2023Quote: ... which were incubated overnight on a rotatory wheel at 4°C with the primary antibodies H4K20me1 (Diagenode; #C15410034; 2 μg), H3K9me3 (Diagenode ...
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bioRxiv - Cancer Biology 2022Quote: ... Shearing was performed in 1.5 ml Bioruptor® Pico Microtubes with Caps (Diagenode Cat# C30010016) with the following cell number ...
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bioRxiv - Molecular Biology 2022Quote: ... The liquid was distributed into 1.5 mL sonication tubes (Diagenode C30010016, 300 µL per tube) and sonicated for 12 min total (24 cycles of 30 s on/off ...
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bioRxiv - Genomics 2023Quote: ... Crosslinked chromatin resuspended in 1 ml LB3 was then sonicated using a BioRuptor Pico (Diagenode) for 30 cycles of 30 seconds on/off ...
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bioRxiv - Cancer Biology 2023Quote: ... Shearing was performed in 1.5 ml Bioruptor® Pico Microtubes with Caps (Diagenode Cat# C30010016) with the following cell number ...
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bioRxiv - Genetics 2024Quote: ... Cell lysates were transferred to Diagenode 15 ml sonication tubes and sonicated (Bioruptor pico, Diagenode) 30 sec ON/ 45 sec OFF at 4°C for 60 cycles (6x10) ...
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bioRxiv - Microbiology 2020Quote: ... was added to the lysate and incubated at 37 °C for 10 min before sonication (2 × 8 bursts of 30 sec on ice using a Diagenode Bioruptor) to shear DNA fragments to a length of 300 to 500 bp ...
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bioRxiv - Biochemistry 2022Quote: ... heated to 95°C for 10 min shaking (1000 rpm) followed by 2 cycles à 20 s of sonication in BioRuptor (Diagenode). Aliquots containing 50 μg protein were transferred into a cartridge and cooled ...
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bioRxiv - Genomics 2022Quote: ... HMW-DNA (25 ng/μl) was separately sheared with 30 kb and 35 kb settings using a Megaruptor 2 instrument (Diagenode SA). However ...
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bioRxiv - Molecular Biology 2019Quote: ... 2% Triton-100 with protease inhibitors) and sonicated using a Bioruptor Pico (Diagenode, 3× cycles 30 sec on / 30 sec off). Sonicated lysates were diluted 2× in 50mM Tris ...
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bioRxiv - Molecular Biology 2019Quote: ... 2% Triton-100 with protease inhibitors and sonication using a Bioruptor Pico (Diagenode, 3× cycles 30 sec on / 30 sec off). 200μg of proteins for each sample were diluted to 1 ml with CoIP Lysis Buffer and incubated with 30μl of anti-FLAG M2 Magnetic Beads (Sigma M8823 ...
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bioRxiv - Plant Biology 2022Quote: ... Approximately 2 ug of genomic DNA was fragmented into 200-300 bp using the Bioruptor UCD-200 sonicator (Diagenode, Denville, NJ). Libraries were constructed using the DNA library prep kit for BGI platform (BGI ...
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bioRxiv - Genomics 2024Quote: DNA concentration was normalized to 50 ng/μl and sheared to 6 Kb fragments using Megaruptor® 2 (Diagenode, Seraing, Belgium). Shearing quality was estimated with TapeStation 4200 (Agilent Technologies ...
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bioRxiv - Molecular Biology 2022Quote: ... Chromatin was sheared in Bioruptor® Pico 15 mL tubes with 300 µL beads (Diagenode C30010017) by 25 cycles of 30 sec on and 30 sec off using a Bioruptor® (Diagenode B01060010) ...
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bioRxiv - Cell Biology 2023Quote: ... for 10 cycles 30 sec ON/30 sec OFF in 1.5 mL Diagenode tubes (Diagenode; #C30010016) to generate 100–400 bp DNA fragments ...
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bioRxiv - Cell Biology 2023Quote: ... for 15 cycles 30 sec ON/30 sec OFF in 1.5 mL Diagenode tubes (Diagenode; #C30010016). Finally ...
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bioRxiv - Cancer Biology 2023Quote: ... for 15 cycles 30 sec ON/30 sec OFF in 1.5 mL Diagenode tubes (Diagenode; #C30010016). Finally ...
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bioRxiv - Cell Biology 2020Quote: ... 600 ng of denatured DNA was incubated with 2 mg of 5-methylcytosine (5-mC) monoclonal antibody ([33D3] Diagenode, Cat No # C15200081) in IP buffer (0.1% SDS ...
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bioRxiv - Genomics 2021Quote: ... Abcam; anti-ERG 2 µg, ab92513, Abcam; anti-H3K4me3 1.4 µg, C15410003, Diagenode; H3K27me3 2.9 µg, C15410069, Diagenode; H3K27ac 1µg, ab4729, Abcam). As wildtype FLI1 or ERG are not expressed in EWSR1-FLI1 or EWSR1-ERG positive EwS cell lines ...
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bioRxiv - Plant Biology 2019Quote: ... The extracts were sonicated in 15 ml tubes in an ice bath using Bioruptor UCD-200 (Diagenode) on high setting ...
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bioRxiv - Cell Biology 2021Quote: ... Resulting fibrils were diluted in PBS to 0.25 mg/mL transferred to sonication tubes (Diagenode Cat# C30010011) and sonicated (Diagenode Bioruptor Pico ...
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bioRxiv - Molecular Biology 2023Quote: ... 300 µL of lysates were added into 1.5 mL Bioruptor Pico microtubes with caps (Diagenode, cat#C30010016), and subsequent sonication was conducted with a Bioruptor Pico sonication device ...
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bioRxiv - Genetics 2021Quote: ChlPseq was performed on 20k FACS sorted MM cells as previously described 44 using H3K27Ac antibodies (Diagenode Cat #C15410196, lot A1723-0041D/2) but with minor modifications on the preparation of input controls ...
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bioRxiv - Molecular Biology 2021Quote: ... Fixed cells were resuspended in 1 ml RIPA Binding Buffer and placed in the Bioruptor Pico (B01060010, Diagenode) for 10 cycles of 30 seconds on ...
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bioRxiv - Immunology 2020Quote: ... and sonicated for 7.5 min (1.5 mL tubes, high power, 30 s on/off cycle, Bioruptor plus, Diagenode). Lysates (20–30 μL ...
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bioRxiv - Genomics 2022Quote: ... and 12 μg of high molecular weight gDNA was sheared to a size distribution between 15 and 20 kbp using the Megaruptor® 2 (Diagenode, Denville, NJ, USA). For each accession ...
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bioRxiv - Molecular Biology 2021Quote: ... The pellet was mechanically disrupted with a wooden stick and transferred to a 1.5 mL TPX microcentrifuge tube (Diagenode), resuspended in 250 µL FA-lysis buffer (with protease inhibitor) ...
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bioRxiv - Molecular Biology 2019Quote: ... The pellet was then resuspended in 600 µl FA lysis buffer using a syringe and a 23G needle and split over 2x 1.5 ml bioruptor pico microtubes (Diagenode). The chromatin was sheared in a bioruptor pico sonicator (Diagenode ...
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bioRxiv - Cell Biology 2021Quote: ... Pelleted cells were resuspended in 1 ml 50 mM Tris pH 7.5 and sonicated in a Bioruptor Twin (Diagenode) on LOW 30 sec ON ...
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Glucocorticoid receptor collaborates with pioneer factors and AP-1 to execute genome-wide regulationbioRxiv - Genomics 2021Quote: ... 164 μL volume was transferred to each of two 1.5 mL Bioruptor® Plus TPX microtubes (Diagenode, Denville, NJ) and sonicated in a Bioruptor® Plus (UCD-300 ...
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bioRxiv - Genetics 2023Quote: ... 3) around 400 μl of the slurry were transferred to 0.6 ml tubes and sonicated in the Bioruptor (Diagenode) at + 4°C for 1 hour on the “high” setting with sonication intervals set to 45 seconds on/15 seconds off ...
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bioRxiv - Neuroscience 2024Quote: ... 350 μL ice-cold TE buffer was added and chromatin was sheared in 1.5 mL microtubes using a Bioruptor Pico sonication device (B01060010, Diagenode) for 3 × 5 cycles (30 s ON/ 30 s OFF ...
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bioRxiv - Microbiology 2023Quote: ... by 12 cycles of 30 sec of sonication and 30 sec of cooling in 1.5 ml Bioruptor tubes (Diagenode), mixed in equimolar proportions ...
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bioRxiv - Developmental Biology 2021Quote: ... Cells were split into 150 μl aliquots in 0.5 ml Eppendorf tubes and sonicated at 4°C for 30 cycles (30 seconds on/off) using a Bioruptor (Diagenode). Aliquots of the same samples were pooled and spun down at 12,000xg for 5 min ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were lysed in 1 ml extraction buffer by three freeze-thaw cycles using liquid nitrogen and by sonication using a Bioruptor (Diagenode) with one 30 s high power pulse ...
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bioRxiv - Cell Biology 2022Quote: ... The cell lysate was transferred to 15 mL Bioruptor Pico tubes along with ~200μL of the corresponding sonication beads (Diagenode C010200031) and sonicated using a Bioruptor Pico (Diagenode B01060010 ...
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bioRxiv - Molecular Biology 2019Quote: ... Either the whole extract was sonicated in a 15 ml bioruptor pico tube with 300 µl of sonication beads (Diagenode) added (Figure 5B-5D ...
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bioRxiv - Molecular Biology 2019Quote: ... the WCL was fragmented as well by sonicating for 3 cycles 30 seconds on / 30 seconds off in 15 ml bioruptor pico tubes containing 300 µl of sonication beads (Diagenode). After sonication ...
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bioRxiv - Molecular Biology 2019Quote: ... treated by RNase A (20 µg/mL) for 1h at 37°C in TE buffer and sonicated 8 min with BioRuptor (Diagenode) (15 sec ON / 15 sec OFF ...
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bioRxiv - Molecular Biology 2022Quote: ... 1X HALT protease inhibitor cocktail) and split into 250 μL volumes in 1.5 mL Bioruptor Plus TPX microtubes (Diagenode, #C30010010). Cells were sonicated at 4° C for 20 minutes using a waterbath sonicator cycling 30 seconds on and 30 seconds off (Bioruptor Pico ...
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bioRxiv - Neuroscience 2020Quote: PFF transduction was conducted at 10 DIV by adding 2 ug/ml (96 well plate and coverslips) or 10 ug/ml (biochemistry) of PFFs diluted in PBS and sonicated with a BioRuptor (Diagenode) bath sonicator for 10 cycles of 30 seconds on ...
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bioRxiv - Molecular Biology 2023Quote: ... 5 ml of thawed OFs were sonicated with a closed-type sonication system (UCD-200T, Biorupter, Diagenode Inc., NJ, USA) and were sequentially centrifuged at 300 g for 10 min (model 5500 with tube 430829 ...
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bioRxiv - Molecular Biology 2019Quote: ... added (Figure 5B-5D) or 2x 300 µl was sonicated in 2x 1.5 ml bioruptor pico microtubes (Diagenode, Figure 6-9). When using the APLP protease inhibitors ...
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bioRxiv - Cancer Biology 2022Quote: ... 200 mM NaCl) supplemented with 100 μg/ml ssDNA and PI and sonicated using Bioruptor Pico sonicator (Diagenode, Denville, NJ, USA). In order to reach DNA fragments in the range of 200-700 bp ...
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bioRxiv - Cancer Biology 2022Quote: ... 50 µL storage buffer was added to chromatin and samples were transferred to 1.5 mL Bioruptor Microtubes with Caps for Bioruptor (Diagenode, Denville, NJ). Samples were then loaded into Pico Biorupter (Diagenode ...
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bioRxiv - Developmental Biology 2019Quote: ... Embryos were kept on ice and processed directly or frozen at −80C in 20 ml of RNAse/DNAse free PBS supplemented with 1× protein inhibitor (Diagenode, Belgium). Embryos younger than late gastrula (younger than E7.5 ...
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bioRxiv - Molecular Biology 2021Quote: ... and 6 μg of DNA was diluted to 20 ng/uL in 300 μL of water in 1.5 mL Bioruptor Pico Microtubes for sonication (Diagenode, Cat # C30010016). DNA was sonicated using a Bioruptor Pico (Diagenode ...
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bioRxiv - Neuroscience 2022Quote: ... PFFs were diluted in 1× PBS to a final concentration of 100 μL/Ml and sonicated with Bioruptor sonication device (Diagenode, Liege, Belgium, #B01020001) at the following settings ...