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Citations for Diagenode :
201 - 250 of 277 citations for PAS Domain Containing Serine threonine Protein Kinase PASK Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2021Quote: ... The IP of the methylated DNA was performed overnight at 4°C using Protein A Dynabeads coated with anti-5mC (Diagenode, C15200081) or anti-IgG (Diagenode ...
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bioRxiv - Developmental Biology 2019Quote: ... Embryos were kept on ice and processed directly or frozen at −80C in 20 ml of RNAse/DNAse free PBS supplemented with 1× protein inhibitor (Diagenode, Belgium). Embryos younger than late gastrula (younger than E7.5 ...
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bioRxiv - Genetics 2021Quote: ... 3 to 7 days later individual colonies were picked and expanded and western blot was used to confirm Cas9 protein expression (Diagenode, C15200203). Cas9 expression in a high expressing line was confirmed by immunohistochemistry (5).
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Heat Shock Factor 1 (HSF1) as a new tethering factor for ESR1 supporting its action in breast cancerbioRxiv - Cancer Biology 2021Quote: ... 30 µg of chromatin and 4 μl of mouse anti-ERalpha monoclonal antibody (C15100066, Diagenode) was used ...
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bioRxiv - Molecular Biology 2021Quote: ... with following appropriate antibodies at 4 °C o/n a rotating wheel: H3K27me3 (Diagenode, C15410195), RING1B (Cell Signaling ...
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bioRxiv - Genetics 2022Quote: ... Immunoprecipitation was performed using 10 μg of chromatin and 2.5 μg of human antibody (H3K4me3 [RRID:AB_2616052, Diagenode C15410003], H3K4me1 [RRID:AB_306847, Abcam ab8895], and H3K27ac [RRID:AB_2637079, Diagenode C15410196]). Final DNA purification was performed using the GeneJET PCR Purification Kit (Thermo Scientific ...
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bioRxiv - Genomics 2023Quote: ... The sample was then incubated with antibodies (AR: Abcam ab133273, FOXA1: Abcam ab23738, H3K27ac: Diagenode 3C15410196 ...
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bioRxiv - Microbiology 2021Quote: 60,000 iATII cells or Calu-3 cells were incubated for 1 h at 4°C with equal protein concentrations of control rabbit IgG (Diagenode, Cat#C15410206) or 1/200 dilution of rabbit anti-ACE2 (Abcam ...
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bioRxiv - Microbiology 2023Quote: ... 10 μg total RNA was incubated overnight at 4 °C with Dynabeads™ Protein G magnetic beads pre-bound with anti-Ψ (Diagenode) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2019Quote: ... ChIP-seq was performed using the protocol previously described (51) with antibodies to H3K27Ac (C15410196, Diagenode) and H3K27me3 (9733S ...
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bioRxiv - Physiology 2021Quote: ... Immunoprecipitation was performed using 10 μg of chromatin and 2.5 μg of antibody (H3K4me3 (Diagenode C15410003), H3K4me1 (abcam ab8895 and H3K27ac (Diagenode C15410196) ...
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bioRxiv - Cancer Biology 2020Quote: ... using 1 µg antibody (anti-H3K4me1, #ab8895, Abcam; anti-H3K27ac, #ab4729, Abcam; anti-H3K4me3, #c15410003, Diagenode). 10% of the sheared chromatins were kept aside as input samples ...
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bioRxiv - Molecular Biology 2022Quote: ... chromatin of each replicate was incubated with 1 μg of antibody against H3K27ac (Diagenode, C15410196, RRID:AB_2637079) or 1 μg of antibody against ONECUT2 (Proteintech ...
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bioRxiv - Genomics 2022Quote: ... Proximity ligated chromatin pellet was sonicated and immunoprecipitated using 5 μg of H3K27ac antibodies (Diagenode, C15410196). Immunoprecipitated fragments were Biotin labeled (Invitrogen ...
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bioRxiv - Systems Biology 2023Quote: ... single-stranded DNA mixture was incubated with 5-mC antibody provided by MagMeDIP-seq Package (Diagenode), followed by 14-16 cycles of library amplification ...
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bioRxiv - Genomics 2023Quote: ... respectively) of the primary antibodies (C15410003 and C15410196 for H3K4me3 and H3K27ac, respectively; Diagenode, Denville, NJ), 0.05% (w/v ...
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bioRxiv - Immunology 2024Quote: ... Beads were blocked with 1 µL of antibody (α-H3K27ac, Diagenode, C15410196; α-H3K4me3, Diagenode, C15410003) in antibody binding buffer for 3-4h at 4°C with rotation ...
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bioRxiv - Cancer Biology 2024Quote: ... 25µg of sheared chromatin was then incubated with 4µg of antibody against H3K4me3 (Diagenode, C15410003-50) H3K36me3 (Abcam ...
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bioRxiv - Cancer Biology 2021Quote: The HiChIP protocol was performed as previously described (Mumbach 2016) using 7.5µg antibody to H3K27ac (Diagenode, C15410196) with the following modifications ...
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bioRxiv - Genetics 2020Quote: ... Immunoprecipitation was performed using 75 µg of chromatin and 5 µg of Cas9 antibody (Diagenode #C15200229-100) or 15 µg of chromatin and 2.5 µg of H3K27ac and H3K9me3 antibody (Active Motif #3913 ...
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bioRxiv - Genetics 2020Quote: ... that had been pre-blocked with 0.5% BSA and mixed with polyclonal CTCF antibody (C15410210-50, Diagenode) or polyclonal H3K9me3 antibody (AB_2532132 ...
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bioRxiv - Genomics 2020Quote: ... The latter aliquot was processed for ChIP using 3 μg anti-H3K4me3 antibody (pAb-003-050, Diagenode) as indicated in the user manual ...
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bioRxiv - Genetics 2022Quote: ... Immunoprecipitation was performed using 10 μg of chromatin and 2.5 μg of human antibody (H3K4me3 [RRID:AB_2616052, Diagenode C15410003] ...
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bioRxiv - Genomics 2023Quote: ... about half of the chromatin extract was used for immunoprecipitation with 5µg of H3K27me3 antibody (C15410069 Diagenode). IP ...
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bioRxiv - Genomics 2020Quote: ... One µg per 100,000 cells of the desired antibody (H3K4me3 C15410003, H3K27ac C15410196, H3K27me3 C15410195, all from Diagenode) is added into each well ...
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bioRxiv - Genetics 2020Quote: ... or 15 µg of chromatin and 2.5 µg of H3K27ac and H3K9me3 antibody (Active Motif #3913, Diagenode #C15410193). Final DNA purification was performed using the GeneJET PCR Purification Kit (Thermo Scientific ...
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bioRxiv - Cancer Biology 2021Quote: ... Adapter-ligated DNA was subjected to immunoprecipitation with a primary monoclonal antibody against 5-methyl cytosine (Diagenode C15200081) using a previously published protocol [71] ...
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bioRxiv - Cancer Biology 2020Quote: ... The sample was then incubated with antibodies (H3K27ac, Diagenode, C15410196; H3K27me3, Cell Signaling 9733S; H3K4me3, Diagenode C15410003 premium) coupled with protein A and protein G beads (Life Technologies ...
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bioRxiv - Molecular Biology 2023Quote: ... CTCF ChIP-seq samples were prepared with 3 x 106 cells and 1 µg CTCF antibody (Diagenode C15410210). ChIP-seq Dilution Buffer (16.7 mM Tris-HCl pH 8.1 ...
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bioRxiv - Plant Biology 2024Quote: ... slides were incubated over night at 4°C with primary-antibody (H3K9me2: Diagenode: C15200154; H3K27me1: Diagenode: C15410045-50) diluted in 1% BSA ...
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bioRxiv - Neuroscience 2021Quote: ... the adaptor-ligated DNA was used for immunoprecipitation (IP) with a mouse monoclonal anti-5-hydroxymethylcytosine antibody (Diagenode, C15200200). For this ...
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bioRxiv - Genomics 2020Quote: ... then incubated with specific antibodies (anti-H3K27me3 Cell Signaling Technology 9733, anti-H3K36me2 CST 2901, anti-H3K27ac Diagenode C15410196), 1.5 million cells of sonicated cell lysate ...
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bioRxiv - Neuroscience 2022Quote: ... Pre-cleared chromatin lysate was immunoprecipitated by a ChIP-grade rabbit polyclonal antibody against histone H3K9me3 (Cat. # C15410193, Diagenode) with end-over-end rotation (4°C ...
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bioRxiv - Cell Biology 2022Quote: ... and DNA fragments (200 - 500 bp) were immunoprecipitated with a mouse monoclonal anti-5hmC antibody (2.5 μg per immunoprecipitation) following the protocol from Diagenode. The DNA-antibody mixture was incubated with magnetic beads overnight at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... 4 µg of chromatin were immunoprecipitated overnight at 4°C with 8 µg of antibody against H3K9me3 (Diagenode, C15410193) previously coupled to protein A/G magnetic beads provided in the kit ...
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bioRxiv - Molecular Biology 2022Quote: ... RNA was then incubated at 4°C overnight with or without 12.5 μg of anti-5hmC antibody (C15220001, Diagenode) in freshly prepared 1X Immunoprecipitation (IP ...
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bioRxiv - Immunology 2022Quote: ... Sonicated chromatin was diluted ten times in ChIP Dilution Buffer and immunoprecipitation was done overnight at 4°C by incubating 2.5 μl (7 μg) of anti-H3K27ac antibody (C15410196; Diagenode) precoated on 25-μl protein A-coated magnetic beads (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2022Quote: ... 2 mg of chromatin lysate was rotated overnight at 4°C with 5 μg antibody: rabbit IgG (Diagenode #C15410206), anti-NIPBL (NIPBL#1 ...
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bioRxiv - Molecular Biology 2023Quote: ... and were directly subjected to immunoprecipitation with corresponding antibodies (H3K4me3: Diagenode, C15410003; H3K27me3: Diagenode, C15410195; CTCF: Millipore 07-792). Library preparation was carried out using NEBNext Ultra II DNA Library Prep kit (NEB E7645) ...
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bioRxiv - Cancer Biology 2023Quote: ... Isolated nuclei from primary fallopian tube secretory epithelial cells bound to concalavin A beads were incubated with primary antibody that detects H3K27ac (0.7μg, Diagenode, RRID:AB_2637079) at 4℃ overnight ...
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bioRxiv - Genomics 2023Quote: ... The sample was then incubated with antibodies (AR: Abcam ab133273, FOXA1: Abcam ab23738, H3K27ac: Diagenode 3C15410196; H3K4me3: Diagenode 3C15410003) coupled with Dynabeads protein A and protein G beads (Life Technologies ...
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bioRxiv - Plant Biology 2024Quote: ... Primary antibody incubation was performed overnight at 4°C with gentle shaking using anti-H3K9me2 (Diagenode Cat. No. C15410060), Anti-Histone H3 (Sigma-Aldrich Cat ...
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bioRxiv - Microbiology 2024Quote: ... Immunoprecipitation was done with 2 µg of chromatin and 4 µg of antibody against H3K9me3 (Diagenode, C15410193, lot A2217P). Sequencing libraries were prepared from 5 ng of immunoprecipitated DNA as previously described [19 ...
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FOXO dictate initiation of B cell development and myeloid restriction in common lymphoid progenitorsbioRxiv - Immunology 2022Quote: ChIPseq was performed as previously described (Bouderlique et al., 2019) using H3K27Ac antibodies (Diagenode Cat #C15410196, lot A1723-0041D/2) and the ThruPLEX DNA-seq (Rubicon Genomics ...
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bioRxiv - Evolutionary Biology 2019Quote: ... single male larvae were selected and processed following the low-input native chromatin immunoprecipitation protocol [72] with H3K9me3 and H4K16ac antibodies from Diagenode. The libraries were prepared using the SMARTer Universal Low Input DNA-seq Kit (Takara ...
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bioRxiv - Plant Biology 2019Quote: ... For 5-mC immunolocalization slides were first incubated over night at 4°C with a monoclonal anti-5-mC-antibody (Diagenode C15200003 ...
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bioRxiv - Molecular Biology 2019Quote: ... or 2 ng (other antibodies) of ChIP-ed DNA was used to prepare DNA libraries with MicroPlex Library Preparation Kit v2 (Diagenode).
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bioRxiv - Microbiology 2022Quote: ... Immunoprecipitation was done on chromatin corresponding to the remaining 10 000 cells using antibodies against H3K4me3 (Diagenode, cat no. C15410003) – 10% of the chromatin was used for input ...
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bioRxiv - Microbiology 2023Quote: ... chromatin-antibody complexes were washed and eluted from the beads using the iDeal ChIP-seq kit for transcription factors (Diagenode) according to the manufacturer’s protocol ...
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bioRxiv - Genetics 2023Quote: ... We assessed two histone marks H3K4me3 and H3K27me3 and the ChIP grade antibodies against for these marks procured from Diagenode (H3K4me3 ...