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Citations for Diagenode :
1 - 50 of 73 citations for Nuclear Protein Extraction since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... and protein extraction beads (Diagenode, NJ, USA) according to the manufacturer instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... homogenization was performed using protein extraction beads (Diagenode) and sonication in the lysis buffer followed by centrifugation to remove solid remains (Diagenode Bioruptor Plus sonicator manufacturer’s protocol for protein extraction from tissue fragments) ...
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bioRxiv - Neuroscience 2023Quote: ... in presence of 50 mg Protein Extraction beads (C20000021, Diagenode); samples were trypsin digested for 2 h 30 min ...
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bioRxiv - Immunology 2020Quote: 1.5 ml Bioruptor Microtubes were filled with 250 mg of Protein Extraction Beads (Diagenode) and filled with RIPA buffer (supplemented with protease and phosphatase inhibitors) ...
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bioRxiv - Molecular Biology 2023Quote: ... Nuclear fraction was sonicated (Bioruptor NextGen, Diagenode) at high amplitude for 10 cycles of 30 sec (on/off ...
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bioRxiv - Molecular Biology 2023Quote: ... Nuclear fraction was sonicated (Bioruptor NextGen, Diagenode) at high amplitude for 10 cycles of 30 sec (on/off ...
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bioRxiv - Cancer Biology 2019Quote: ... Nuclear lysates were prepared by sonication (Bioruptor (Diagenode), low amplitude ...
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bioRxiv - Cell Biology 2023Quote: ... Nuclear extracts were sonicated on a Bioruptor Pico (Diagenode) for 10 cycles 30 sec ON/30 sec OFF in 1.5 mL Diagenode tubes (Diagenode ...
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bioRxiv - Cell Biology 2023Quote: ... Lysates were sonicated for 10 minutes using a Bioruptor device together with protein extraction beads (Diagenode, Denville, NJ). Each lysate was diluted with 1080µl 50mM Tris pH7.4 ...
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bioRxiv - Developmental Biology 2020Quote: ... The nuclear lysate was sonicated using a Bioruptor sonicator (Diagenode) for 20 cycles of 30 sec on/30 sec off ...
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bioRxiv - Cancer Biology 2023Quote: ... Samples were taken up in 100 μL LYSE buffer and mixed with 50 mg of protein extraction beads (Diagenode). Samples were sonicated with 10-20 cycles at 30sec ON/OFF in a Diagenode Bioruptor Plus until complete tissue disruption was observed ...
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bioRxiv - Cancer Biology 2022Quote: ... RNA extraction beads (Diagenode) in a Diagenode Bioruptor Plus sonicator ...
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bioRxiv - Plant Biology 2019Quote: ... The nuclear lysate was subjected to sonication in a Bioruptor (Diagenode), 10 cycles 30’ On ...
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bioRxiv - Neuroscience 2022Quote: ... A modified protocol for on-beads digest (sonication for 10*30s in a Bioruptor sonicator in presence of 50 mg Protein Extraction beads, C20000021, Diagenode) was used for tissue samples followed by a 3.5h digestion step ...
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bioRxiv - Cancer Biology 2021Quote: ... Nuclear Fractions were further sonicated in a Biorupter Plus sonication device (Diagenode) at 4 °C for 15 cycles of 60 seconds ON ...
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bioRxiv - Neuroscience 2020Quote: ... Purified nuclear fraction was subjected to sonication using Bioruptor Pico (Diagenode, Belgium) to obtain DNA fragments of 200-500 bp ...
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bioRxiv - Immunology 2020Quote: ... Nuclear lysates were isolated as previously described22 and sonicated with a Bioruptor (Diagenode) for 8 cycles (GM-BMDMs ...
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bioRxiv - Neuroscience 2024Quote: ... The nuclear fraction was isolated and subjected to sonication (Diagenode Bioruptor PICO B01080010) to break the DNA into 200-700 bp fragments (4 cycles 30/30 on/off time ...
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bioRxiv - Microbiology 2019Quote: ... The nuclear membrane was disrupted by sonication in a Bioruptor water bath sonicator (Diagenode, 30 s on ...
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bioRxiv - Cancer Biology 2021Quote: ... Nuclear extracts were collected and sonicated for 10 minutes to obtain 300bp chromatin fragments (Diagenode Bioruptor Pico). Equal volumes of sheared chromatin were immunoprecipitated with rabbit AR-V7 antibody (RevMab 31-1109-00) ...
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bioRxiv - Cancer Biology 2023Quote: ... incubated in RNA Extracol with RNA extraction beads (Diagenode), sonicated using Bioruptor Plus (Diagenode) ...
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bioRxiv - Immunology 2024Quote: Total RNA extraction was performed from PBMC by Diagenode (Belgium). RNA samples were quantified using QubitTM RNA BR Assay Kit (Thermo Fisher Scientific ...
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bioRxiv - Evolutionary Biology 2020Quote: ... DNA extractions were then fragmented with the Bioruptor Pico (Diagenode, Belgium) using 7 cycles of 30s fragmentation and 60s cooling ...
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bioRxiv - Molecular Biology 2021Quote: ... the nuclear lysate was sonicated for 3 cycles of 30 seconds ON / 30 seconds OFF using a Bioruptor Pico (Diagenode) to ensure solubilisation of chromatin and then spun 18,000g for 10 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... At the last step of the protocol both nuclear and cytoplasmic samples were sonicated for 10 cycles (30 seconds on / 30 seconds off) using the Bioruptor Pico (Diagenode). For the preparation of the nuclear extraction and fractionation buffer the reagents used were HEPES (Sigma-Aldrich ...
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bioRxiv - Immunology 2024Quote: ... Nuclear extracts were prepared 21 and chromatin DNA was sonicated using Bioruptor Pico (Diagenode, 8 cycles, 30s on/off bursts) to generate DNA fragments ∼200-700 bp ...
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bioRxiv - Cancer Biology 2023Quote: ... nuclei were extracted from 50,000 cells and the nuclear pellet was resuspended in the Tn5 transposase reaction mix (Diagenode, Cat# C01070012-30). The transposition reaction was incubated at 37°C for 30 min ...
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bioRxiv - Microbiology 2024Quote: ... an internal inhibition extraction control (Xeno™ DNA control or Diagenode IPC, Thermofisher) was added to each sample.
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bioRxiv - Plant Biology 2022Quote: ... Chromatin extraction and chromatin shearing were performed using a Universal Plant ChIP-seq kit (Diagenode) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... Dynal Protein A magnetic beads (Diagenode) and ChIP-graded anti-myc antibody (ab9132 ...
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bioRxiv - Biochemistry 2023Quote: ... Unblocked protein A beads (Diagenode, C03020003) were used to pulldown the antibody-protein complexes ...
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bioRxiv - Cancer Biology 2021Quote: ... The DNA-protein-antibody complexes were captured by DiaMag protein A-coated magnetic beads (Diagenode; Cat#: C03010020) by incubating at 4 °C for 2 hours ...
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bioRxiv - Cancer Biology 2021Quote: ... The DNA-protein-antibody complexes were captured by DiaMag protein A-coated magnetic beads (Diagenode; Cat#: C03010020) by incubating at 4 °C for 2 hours ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were lysed in 1 ml extraction buffer by three freeze-thaw cycles using liquid nitrogen and by sonication using a Bioruptor (Diagenode) with one 30 s high power pulse ...
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bioRxiv - Cancer Biology 2023Quote: ... For protein extractions, the chromatin fraction was sonicated for 4 min (30s-on/30s-off, “high power”) on the Bioruptor Plus (Diagenode). Proteins’ content was quantified with the Thermo Fisher Scientific− BCA Protein Assay Kit (Thermo Fisher Scientific).
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bioRxiv - Neuroscience 2022Quote: ... Chromatin-protein complexes were immunoprecipitated with protein A/G magnetic beads and washed sequentially according to the manufacturer (Diagenode, C01010055). DNA fragments were then purified using IPure beads v2 (Diagenode ...
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bioRxiv - Developmental Biology 2019Quote: ... overnight and Protein A magnetic beads (Diagenode C03010020) the following day for 2 hours ...
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bioRxiv - Developmental Biology 2019Quote: ... ChIP was performed using Protein G beads (Diagenode) O/N using a H3K27ac antibody (Active Motif ...
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bioRxiv - Microbiology 2023Quote: ... magnetic beads coated with DiaMag protein A (Diagenode), and antibodies against epitope tags (HA or MYC ...
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Regulation of transcription patterns, poly-ADP-ribose, and RNA-DNA hybrids by the ATM protein kinasebioRxiv - Molecular Biology 2023Quote: ... 50mg patient tissue RNA was extracted in an RNase free environment using RNA Extraction kit for Bioruptor Plus (Diagenode Cat# C20000010). The additional protocol listed for DNaseI treatment was followed for 10ug of extracted RNA per sample ...
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bioRxiv - Plant Biology 2021Quote: ... Protein extract was sonicated using a cooled Biorupter (Diagenode) for 10 minutes using high power with 30 seconds on 30 seconds off cycle ...
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bioRxiv - Plant Biology 2020Quote: ... magnetic beads (DiaMag protein A-coated magnetic beads: Diagenode) were incubated with each antibody overnight ...
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bioRxiv - Molecular Biology 2023Quote: ... Total proteins were sonicated on a Bioruptor Plus (Diagenode) (position high ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein extracts were finally sonicated in a Bioruptor (Diagenode) for 3 cycles at high intensity ...
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bioRxiv - Bioengineering 2024Quote: Unloaded Tn5 transposase proteins were acquired from Diagenode (#C01070010-10). Tn5 transposase was then loaded with oligos containing mosaic ends incorporating 13 base-pair unique molecular identifiers (UMIs ...
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bioRxiv - Molecular Biology 2019Quote: ... 1.2 μg total protein was analysed with Cas9 (Diagenode C15310258, 1:400) and GAPDH (EMD MAB374 ...
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bioRxiv - Cancer Biology 2020Quote: ... followed by immunoprecipation of immunocomplexes with protein-A agarose beads (Diagenode; C03020002) previously blocked with 0.5% BSA for 1 hr at 4°C ...
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bioRxiv - Developmental Biology 2022Quote: ... Sheared chromatin was thereafter immunoprecipitated with Protein A-coated magnetic beads (Diagenode) preincubated with 4.5 μg/IP MED1 antibody (Bethyl A300-793A ...
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bioRxiv - Molecular Biology 2023Quote: ... Antibody-bound chromatin was then coupled to protein A beads (Diagenode, C03020002) for three hours at 4° C ...
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bioRxiv - Molecular Biology 2020Quote: ... The Low cell# ChIPkit protein A × 48 was from Diagenode (Denville, NJ, USA). TaqMan probes for mRNA expression and Chemiluminescence western blot detection reagent were from Thermo Fisher Scientific (Waltham ...