Labshake search
Citations for Diagenode :
301 - 350 of 423 citations for Mouse Oligodendrocyte transcription factor 1 OLIG1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2021Quote: ... ONT libraries were prepared using the EXP-NBD103 and SQK-LSK109 kits according to the manufacturer’s instructions and using 4 µg of 40Kb sheared DNA (Megaruptor, Diagenode) as input ...
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bioRxiv - Genomics 2021Quote: ... 8 µg of adequately sheared chromatin was subjected to immunoprecipitation using iDeal ChIP-seq kit for Histones (Diagenode, C01010050) with 2 µg of antibodies against histone modifications or 10 µg of custom RPB1 antibody ...
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bioRxiv - Developmental Biology 2022Quote: ... One hundred fifty microliters of Hank’s buffered salt solution with 1x protease inhibitor cocktail (in True MicroChIP kit, Diagenode) was added ...
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bioRxiv - Molecular Biology 2019Quote: ... RNA was used as template for library construction using the CATS mRNA/small RNA kit (C05010043 and C05010040, Diagenode) with slight modifications to protocol i.e. ...
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bioRxiv - Genomics 2021Quote: ... Libraries were prepared from 10 ng of double-stranded purified DNA using the MicroPlex Library Preparation kit v2 (Diagenode) following the manufacturer’s protocol with some modifications ...
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bioRxiv - Genomics 2021Quote: ... Global DNA methylation profiling was generated with the Infinium MethylationEPIC BeadChip Kit (Queensland University of Technology and Diagenode, Austria). The first batch contained only males and were randomized for timepoint and age ...
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bioRxiv - Pathology 2022Quote: ... library was prepared using the EXP-NBD103 and SQK-LSK108 kits according to the manufacturer’s instructions and starting with 3 μg of 20 kb sheared DNA (Megaruptor, Diagenode) as input ...
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bioRxiv - Microbiology 2022Quote: ... July 2020 release using the SMRTbell Express Template Prep Kit v2.0 with the following modifications: genomic DNA was sheared using Speed 34 on the Megaruptor 3 (Diagenode) and an additional size selection step of 6-50kb fragments on the Blue Pippin (Sage Science ...
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bioRxiv - Plant Biology 2023Quote: ... rRNA depleted RPFs were directly used as input for the D-plex small RNA-seq kit (Diagenode cat#C05030001), according to manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... 25 ng of RNA in 8 µL water was input to the D-Plex Small RNA kit (Diagenode, C0503000) and libraries were prepared according to the manufacturer recommendations with the exception that 9 cycles of PCR were carried out ...
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bioRxiv - Cell Biology 2020Quote: ... Chromatin was sonicated to approximately 1 nucleosome using a Bioruptor waterbath sonicator (Diagenode). Chromatin of ∼5×106 cells was incubated with 3ug antibody (RNAPII ...
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The G2-phase enriched lncRNA SNHG26 is necessary for proper cell cycle progression and proliferationbioRxiv - Molecular Biology 2021Quote: ... Overnight IP was performed using 1 μg of anti-H3K4me3 (Diagenode, C1541003-50), 2 μg anti-H3K27me3 (Diagenode ...
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bioRxiv - Genomics 2020Quote: ... and probed with rabbit pAb CRISPR/Cas9 (C15310258, Diagenode, Liège, Belgium, 1:5000), rabbit pAb GFP (ab290 ...
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bioRxiv - Genomics 2021Quote: ... Primary antibodies against the following proteins were applied: H3K4me1 (1:100, C15410037, Diagenode), H3K27ac (1:100 ...
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bioRxiv - Microbiology 2021Quote: ... 1 μg of gDNA was fragmented by mechanical shearing using a bioruptor (Diagenode) using the shearing profile 30 s ON ...
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bioRxiv - Evolutionary Biology 2024Quote: ... and sheared between 15kb to 20 kb using the Megaruptor 1 system (Diagenode). For Morpho amathonte ...
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bioRxiv - Microbiology 2019Quote: ... pneumophila in the samples was detected by diagnostic PCR using primers and probes of the R-DiaLegTM kit (Diagenode, Belgium). The PCRs were prepared in a final volume of 20 μL by adding the Master Mix 5X (TaqMan Probe LC2.0 ...
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bioRxiv - Developmental Biology 2020Quote: ... the bisulfite conversion ratio was calculated using spike-in controls provided by Premium Reduced Representation Bisulfite Sequencing (RRBS) Kit (Diagenode) (supplementary Table S2 ...
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bioRxiv - Developmental Biology 2020Quote: ... samples were sent to the Genomics Unit of the Centre for Genomic Regulation (PRBB, Barcelona) where RRBS libraries were prepared using the Premium Reduced Representation Bisulfite Sequencing (RRBS) Kit (Diagenode), and sequenced ton an Illumina HiSeq 2000 platform in 50-bp single-end mode ...
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bioRxiv - Genetics 2020Quote: ... 50ng of immunoprecipitated DNA or input sample were used to prepare sequencing libraries using the iDeal Library Preparation kit (Diagenode), according to manufacturer instructions ...
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bioRxiv - Genetics 2019Quote: 0.5 × 106 frozen cells were resuspended in 250 µl buffer iL1 with proteinase inhibitors cocktail (iDeal ChIP-seq Kit for Histones, Diagenode) and incubated for 10 min at 4ºC on the Bohemian wheel ...
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bioRxiv - Genomics 2019Quote: ... Chromatin from hMSCs and differentiated chondrocytes were extracted using the Diagenode iDeal histone ChIP-seq kit (Diagenode SA, Ougrée, Belgium). Extracted chromatin was sonicated using a Diagenode Bioruptor Standard or Bioruptor Pico to an average size of 200-500bp ...
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bioRxiv - Cancer Biology 2022Quote: Tumour samples with optimal chromatin fragment size (200-500 bp) were immunoprecipitated using the iDeal ChIP-seq Kit for Histones (Diagenode). Magnetic immunoprecipitation of sheared DNA-chromatin complexes (500ng ...
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bioRxiv - Developmental Biology 2022Quote: ... in VFG media for 10 minutes at room temperature followed by a 5-minute quench with glycine (in True MicroChIP kit, Diagenode) at room temperature ...
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bioRxiv - Molecular Biology 2019Quote: ... Approximately 500 ng of total RNA was used for NGS libraries preparation with CATS mRNA-seq kit (Diagenode, Liege, Belgium) according to the manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2019Quote: ... Then DNA was recovered by AMPure XP Beads and used for methylated DNA immunoprecipitation (MeDIP) using the Magnetic Methylated DNA Immunoprecipitation Kit (Diagenode) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2019Quote: ... Libraries for reduced representation bisulfite sequencing (RRBS) were prepared from 100 ng DNA per sample using the Premium RRBS Kit (Diagenode). Final libraries were purified twice using Agencourt AMPure XP beads (Beckman Coulter ...
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bioRxiv - Cancer Biology 2021Quote: H3K27ac chromatin immunoprecipitation (ChIP) experiments were performed as previously described12 using the iDeal ChIP-seq kit for histones (Cat# C01010171, Diagenode) and the H3K27ac rabbit polyclonal antibody (Abcam Cat# ab4729 ...
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bioRxiv - Immunology 2021Quote: ... Chromatin was prepared from 40–50 million cells using Diagenode Chromatin shearing optimization kit (Cat no. C01010055) and further subjected to sonication using Bioruptor sonicator (Diagenode) to obtain chromatin fragments of 100–500 bps ...
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bioRxiv - Genomics 2021Quote: ... ChIP-seq for H3K4me3 and H3K27ac in PEER cell line was generated using MicroPlex Library Preparation Kit (Diagenode, Liege, Belgium) according to the BLUEPRINT protocol (http://dcc.blueprint-epigenome.eu/#/md/methods) ...
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bioRxiv - Genetics 2020Quote: ... 500 ng of each sample was converted into a sequencing-ready library using the CATS RNA-seq kit with rRNA depletion (Diagenode). The RNA integrity ...
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bioRxiv - Molecular Biology 2019Quote: ... or 2 ng (other antibodies) of ChIP-ed DNA was used to prepare DNA libraries with MicroPlex Library Preparation Kit v2 (Diagenode).
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bioRxiv - Plant Biology 2022Quote: ... 250 pg of immunoprecipitated (IP) or genomic (INPUT) DNA were used to prepare libraries with the MicroPlex Library Preparation kit v2 (Diagenode). Quality of libraries was validated using Agilent / Advanced Analytical fragment analyzer ...
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bioRxiv - Molecular Biology 2023Quote: Libraries were then prepared from 10 ng of RPFs using the D-Plex Small RNA-Seq kit for Illumina (Diagenode) and the D-Plex Unique Dual Indexes for Illumina – set A (Diagenode) ...
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bioRxiv - Cell Biology 2023Quote: ... DNA was purified as for ChIP-seq samples following the Diagenode iPure protocol using reagents from the Auto iDeal ChIP-seq kit (Diagenode). DNA quantification ...
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bioRxiv - Neuroscience 2023Quote: ... and 8 μl was used for small RNA libraries construction by the D-Plex Small RNA-seq Kit (Diagenode C05030001). Indexes were attached using the D-Plex Single Indexes for Illumina - Set A (Diagenode C05030010 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Several libraries were constructed using the Ligation Sequencing Kit (Nanopore LSK109) using DNA sheared to different size using a megaRuptor (Diagenode) to optimise yield and contiguity ...
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bioRxiv - Molecular Biology 2020Quote: ... followed by immunostaining with Cas9 monoclonal antibody 4G10 (Diagenode cat#: C15200216; 1:200 dilution) with gentle shaking for 2h at room temperature ...
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bioRxiv - Molecular Biology 2019Quote: ... The following antibodies were used for western blot analysis: Cas9 (Diagenode C15310258, 1:1000) TCF4 (Abcam ab217668 ...
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The epigenomic landscape regulating organogenesis in human embryos linked to developmental disordersbioRxiv - Genomics 2019Quote: ... 1% SDS and protease inhibitor cocktail) and sonicated under prior optimised conditions (Diagenode Bioruptor). Sufficient sample was prepared to allow in parallel immunoprecipitation for H3K4me3 ...
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bioRxiv - Neuroscience 2019Quote: ... Nuclei diluted in 1 ml RIPA buffer were sonicated using Bioruptor (Diagenode, Denville, NJ) for 6 cycles of 5 minutes (60 seconds on/30 seconds off ...
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bioRxiv - Microbiology 2023Quote: ... bacteria were bath-sonicated for 1 min at medium amplitude on a Bioruptor (Diagenode) to break bacterial chains and plated on plates with suitable antibiotics to select for transformants.
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bioRxiv - Molecular Biology 2019Quote: ... Approximately 10 ng of bisulfite-treated single-stranded DNA was directly used for NGS libraries preparation with CATS DNA-seq Kit v1.0 (Diagenode, Liege, Belgium) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... using the Capture and Amplification by Tailing and Switching method (CATS RNA-seq Kit v2 x24, Diagenode Cat# C05010041, Leige, Belgium). 10ng RNA was used as input and the dephosphorylation step omitted to select for 3’-OH RNA species (miRNA) ...
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bioRxiv - Plant Biology 2022Quote: ... The crosslinking was performed by vacuum infiltration (~ –950 millibars) for 20 min and ChIP-qPCR were performed using Diagenode’s Universal Plant kit (Diagenode, Seraing, Belgium). The shared chromatin was immunoprecipitated using anti-GFP antibody (Abcam 290) ...
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bioRxiv - Neuroscience 2023Quote: ... and 20 ng of BH RNAs were used for small RNA library construction by the D-Plex Small RNA-seq Kit (Diagenode C05030001). D-Plex Single Indexes for Illumina - Set A (DiagenodeC05030010 ...
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bioRxiv - Plant Biology 2022Quote: ... A HiFi library was then prepared according to the “Procedure & Checklist - Preparing HiFi SMRTbell® Libraries using SMRTbell Express Template Prep Kit 2.0” manual with an initial DNA fragmentation by Megaruptor 3 (Diagenode, Belgium) and final library size binning into defined fractions by SageELF (Sage Science ...
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bioRxiv - Cell Biology 2023Quote: ... a total of 10 ng of dsDNA was purified using the MicroPlex Library Preparation kit v3 (C05010001, Diagenode s.a., Seraing, Belgium), according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... Libraries were constructed from 500 pg total RNA using D-Plex Small RNA-seq Kit for Illumina and Single Indexes for Illumina - Set B (Diagenode, C05030001). RIN aXer cell sorting was determined to be between 1.2 and 7.2 (Bioanalyzer 6000 ...
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Regulation of transcription patterns, poly-ADP-ribose, and RNA-DNA hybrids by the ATM protein kinasebioRxiv - Molecular Biology 2023Quote: ... 50mg patient tissue RNA was extracted in an RNase free environment using RNA Extraction kit for Bioruptor Plus (Diagenode Cat# C20000010). The additional protocol listed for DNaseI treatment was followed for 10ug of extracted RNA per sample ...