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Citations for Diagenode :
101 - 150 of 441 citations for Mouse NLR Family CARD Domain Containing 4 NLRC4 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... the different antibodies were previously incubated at 4°C with protein G-coated magnetic beads (DiaMag, Diagenode), protease inhibitor cocktail and 0.1% BSA ...
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bioRxiv - Molecular Biology 2023Quote: ... Samples were sonicated for 4 min (20 seconds on, 40 seconds off) using a Bioruptor Pico (Diagenode). After sonication samples were cleared with 1% triton X-100 and pelleted (16000 x g for 5 min at 4 °C) ...
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bioRxiv - Neuroscience 2021Quote: Cells were lysed in radioimmunoprecipitation assay (RIPA) buffer containing protease and phosphatase inhibitor cocktails and sonicated for 1 min with the Bioruptor sonication device (Diagenode). Cell lysates were centrifuged at 13,000 × g for 10 min at 4 °C ...
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bioRxiv - Molecular Biology 2020Quote: ... the pellets were resuspended in 300 μL ChIP lysis buffer containing 1 mM PMSF and sonicated for 15 min (30s ON - 60s OFF) in a Bioruptor (Diagenode). The lysates were centrifuged at 7000 rpm for 15 min at 4°C ...
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bioRxiv - Molecular Biology 2019Quote: ... the WCL was fragmented as well by sonicating for 3 cycles 30 seconds on / 30 seconds off in 15 ml bioruptor pico tubes containing 300 µl of sonication beads (Diagenode). After sonication ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 % Triton X-100) supplemented with 1 x HALT in a 15 ml Bioruptor tube containing one scoop of sonication beads (Diagenode) and sonicated in Bioruptor Pico (Diagenode ...
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bioRxiv - Cell Biology 2023Quote: ... the pellets were resuspended in 300 μl ChIP lysis buffer containing 1 mM PMSF and sonicated for 15 min (30s ON–60s OFF) in a Bioruptor (Diagenode). The lysates were centrifuged at 7000 rpm for 15 min at 4°C ...
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bioRxiv - Plant Biology 2023Quote: ... The remaining pellet was re-suspended in 300 µl of lysis buffer containing 1% SDS and sonicated using a bioruptor (Diagenode) at 4°C (15 sec ON/OFF for 5-min and repeated for 5 cycles) ...
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bioRxiv - Molecular Biology 2023Quote: ... The eluate was transferred to a new tube containing 100 μl of TE buffer and then incubated with proteinase K (Diagenode) at 55 °C for 1 h ...
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bioRxiv - Cancer Biology 2023Quote: ... were lysed in buffer (50 mM Tris-HCl, pH 7.8) containing 1% SDS and 0.1 M dithiothreitol and sonicated (Diagenode Bioruptor Plus). Number of sonication cycles was established based on a visual sample clarity assessment ...
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bioRxiv - Molecular Biology 2021Quote: ... 1μL SYBR green 10X (Diagenode kit), 1.3 μL of molecular biology grade water and 5μL of 2xPCR NEB master mix ...
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bioRxiv - Genetics 2020Quote: ... using the LowCell# ChIP kit (Diagenode). Illumina sequencing libraries were generated using the Accel-NGS 2S Plus DNA Library Kit (Swift Biosciences) ...
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bioRxiv - Developmental Biology 2022Quote: ... and the iDeal ChIP-kit (Diagenode) for MED1 according to the manufacturer’s protocols with some modifications as described below ...
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bioRxiv - Developmental Biology 2023Quote: ... the iDeal ChIP-Seq kit (Diagenode) was used according to manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... the µChipmentation kit for histones (Diagenode) was used for chromatin immunoprecipitation and ChIP-Seq library preparation (Supplementary table 2).
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bioRxiv - Genomics 2024Quote: ... using a Microplex v3 kit (Diagenode).
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bioRxiv - Developmental Biology 2023Quote: ... Immunolabeling of Cas9 protein by monoclonal anti-mouse Cas9 antibody at 1:500 (4G10; Diagenode) was blocked in PBS Super Block (37580 ...
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bioRxiv - Developmental Biology 2024Quote: ... The sonication of samples was then performed at 4°C using a Bioruptor® Plus sonication device (Diagenode) at high-intensity settings ...
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bioRxiv - Plant Biology 2024Quote: ... slides were incubated over night at 4°C with primary-antibody (H3K9me2: Diagenode: C15200154; H3K27me1: Diagenode: C15410045-50) diluted in 1% BSA ...
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bioRxiv - Physiology 2023Quote: ... and 500-µl volume) in a water bath sonicator set at 4°C (Bioruptor 300. Diagenode, Denville, NJ). After centrifuging at 10,000g for 10 mins at 4°C ...
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bioRxiv - Genomics 2021Quote: ... 1 mL RIPA and 1 mL RIPA containing resuspended liver was placed into a 15 mL tube with 800 mg of Diagenode sonication beads (Diagenode, C01020031). This tube was sonicated for 30 cycles of 30s on and 30s off using a Bioruptor Pico ...
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bioRxiv - Cell Biology 2023Quote: ... Each chromatin sample containing about 6,000 cells was incubated with 1 µg of anti-H3K27me3 antibody (Diagenode, C15410195, lot. A1811-001P). ChIP was performed using the automated platform SX-8G IP-Star platform (Diagenode ...
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bioRxiv - Molecular Biology 2021Quote: ... using 3 to 4 cycles of 15 sec on : 30 sec off at low intensity in a Bioruptor (Diagenode). After recovery of the NEXSON-isolated nuclei by centrifugation (1000g ...
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bioRxiv - Immunology 2021Quote: ... sonicated at 4°C with 3 cycles of 30 s on 30 s off in a Bioruptor Pico (Diagenode) and clarified by centrifugation at 12,000 × g ...
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bioRxiv - Genomics 2020Quote: ... For single crosslinked cells nuclei were sonicated for 4 cycles of 30s on/30s off using a Picoruptor (Diagenode). Immunoprecipitation was carried out overnight at 4°C using 2 μg of RUNX1 antibody (ab23980 ...
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bioRxiv - Immunology 2020Quote: ... sonicated at 4°C with 3 cycles of 30 s on 30 s off in a Bioruptor Pico (Diagenode) and clarified by centrifugation at 12,000 xg ...
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bioRxiv - Molecular Biology 2022Quote: ... RNA was then incubated at 4°C overnight with or without 12.5 μg of anti-5hmC antibody (C15220001, Diagenode) in freshly prepared 1X Immunoprecipitation (IP ...
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bioRxiv - Immunology 2022Quote: ... Sonicated chromatin was diluted ten times in ChIP Dilution Buffer and immunoprecipitation was done overnight at 4°C by incubating 2.5 μl (7 μg) of anti-H3K27ac antibody (C15410196; Diagenode) precoated on 25-μl protein A-coated magnetic beads (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2022Quote: ... 2 mg of chromatin lysate was rotated overnight at 4°C with 5 μg antibody: rabbit IgG (Diagenode #C15410206), anti-NIPBL (NIPBL#1 ...
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bioRxiv - Cell Biology 2023Quote: ... Chromatin was fragmented by sonication at 4°C using a Bioruptor 300 (Diagenode; 45 cycles, 30s on/30s off). Immunoprecipitation used 4 μg ChIP validated antibody against NF-κB p65 (Millipore ...
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bioRxiv - Biochemistry 2023Quote: ... Lysates were sonicated for 5 cycles of 30 s on/off (2.5 min total sonication time) at 4°C in a Bioruptor Plus sonication system (Diagenode) and pelleted by centrifugation at 8000 RPM for 15 min at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were incubated with mixing for 30 min at 4°C before sonicating with a Bioruptor® Pico (Diagenode). Lysates were then spun for 10 min at 15,000 g ...
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bioRxiv - Microbiology 2023Quote: ... 40 mM 2-Chloroacetamide, 50 mM HEPES pH 8.5), boiled (5 min, 95 °C) and sonicated (5x60 s, 4 °C) (Bioruptor, Diagenode). For the intracellular proteomes ...
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bioRxiv - Plant Biology 2024Quote: ... Primary antibody incubation was performed overnight at 4°C with gentle shaking using anti-H3K9me2 (Diagenode Cat. No. C15410060), Anti-Histone H3 (Sigma-Aldrich Cat ...
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bioRxiv - Molecular Biology 2024Quote: ... using 3 to 4 cycles of 15 sec on : 30 sec off at low intensity in a Bioruptor (Diagenode). After recovery of the NEXSON-isolated nuclei by centrifugation (1000g ...
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bioRxiv - Microbiology 2024Quote: ... Immunoprecipitation was done with 2 µg of chromatin and 4 µg of antibody against H3K9me3 (Diagenode, C15410193, lot A2217P). Sequencing libraries were prepared from 5 ng of immunoprecipitated DNA as previously described [19 ...
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bioRxiv - Molecular Biology 2021Quote: ... the Microplex Library Preparation kit V2 (Diagenode) was used for ChIP-Seq library construction following the manufacturer recommendations ...
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bioRxiv - Genomics 2021Quote: The protocol for Premium RRBS kit (Diagenode) library preparation included enzymatic digestion of 100 ng of gDNA with MspI ...
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bioRxiv - Immunology 2020Quote: ... DNA was purified using IPure kit (Diagenode) according to the manufacturer’s protocol ...
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bioRxiv - Genetics 2023Quote: ... using the iDeal ChIP-seq kit (Diagenode). Libraries were prepared using the Ovation Ultralow V2 DNA-Seq library preparation kit (NuGen ...
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bioRxiv - Cancer Biology 2021Quote: ... lysed in 1% SDS containing buffer supplemented with 1x protease and phosphatase inhibitors, and sonicated for 4 x 10 cycles (30 sec on, 30 sec off) in temperature-controlled Bioruptor 300 (Diagenode). Debris was removed by centrifuging at 14,000 rpm at 4°C ...
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bioRxiv - Molecular Biology 2020Quote: ... was then added at a final concentration of 1 % for 15 min at RT and samples were sonicated over 20 min (10 × 30 s pulses) at 4°C to cleave genomic DNA (Bioruptor, Diagenode). Anti-MAZ antibody was from Bethyl Laboratories (A301-652A) ...
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bioRxiv - Developmental Biology 2021Quote: ... Cells were split into 150 μl aliquots in 0.5 ml Eppendorf tubes and sonicated at 4°C for 30 cycles (30 seconds on/off) using a Bioruptor (Diagenode). Aliquots of the same samples were pooled and spun down at 12,000xg for 5 min ...
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bioRxiv - Developmental Biology 2021Quote: ... Tubes were sonicated for 10 mins (sonication cycle: 30 sec ON, 30 sec OFF) at 4 °C in a Bioruptor ultrasonication bath (Diagenode) at high energy setting ...
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bioRxiv - Cancer Biology 2020Quote: ... Immunoprecipitation was performed using IP star robot at 4°C (indirect 200µl method, SX-8G IP-Star® Compact Automated System, Diagenode) with an anti-BrdU antibody (10μg ...
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bioRxiv - Biochemistry 2022Quote: ... incubated at 95°C for 5 min and sonicated at 4°C for 2 cycles of 20 s at high intensity level using a BioRuptor (Diagenode). Samples were clarified by centrifugation at 16,000 x g for 15 min ...
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bioRxiv - Developmental Biology 2020Quote: ... Tubes were sonicated for 10 mins (sonication cycle: 30 sec ON, 30 sec OFF) at 4 °C in a Bioruptor ultrasonication bath (Diagenode) at high energy setting ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells were sonicated at 4° C for 20 minutes using a waterbath sonicator cycling 30 seconds on and 30 seconds off (Bioruptor Pico, Diagenode). Sonicated lysates were cleared by centrifugation at 18,000 g for 10 minutes at 4° C ...
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bioRxiv - Molecular Biology 2020Quote: ... with minor modifications: Cells were resuspended in PBS at 4°C after flow cytometry sorting and incubated with ChIP Crosslink Gold (Diagenode #C01019027 ...
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bioRxiv - Plant Biology 2019Quote: ... For 5-mC immunolocalization slides were first incubated over night at 4°C with a monoclonal anti-5-mC-antibody (Diagenode C15200003 ...