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Citations for Diagenode :
151 - 200 of 308 citations for Human Chemokine C X C Motif Receptor 5 CXCR5 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: ... incubated at 95°C for 10 min and further sonicated for 15 cycles à 30 s at high intensity level and 4°C using Bioruptor®Plus (Diagenode, Liege, Belgium). Protein concentrations were determined by measuring the tryptophan fluorescence at an emission wavelength of 350 nm using 295 nm for excitation with an Infinite® M1000 PRO microplate reader (Tecan ...
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bioRxiv - Cancer Biology 2023Quote: ... boiled at 95°C for 10min and then DNA was sheared by sonication (Bioruptor® Pico, Diagenode; 4 cycles of 30s ON + 90s OFF). In both cases ...
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bioRxiv - Evolutionary Biology 2023Quote: ... a maximum of 600 ng of DNA per accession was sheared at 4°C to a target average length of 400 bp using a Bioruptor® Pico sonication device (Diagenode, Denville, NJ, USA). Illumina libraries were prepared using a modified KAPA protocol with the KAPA LTP Library Preparation Kit (Roche ...
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bioRxiv - Molecular Biology 2023Quote: ... boiled for 5 min at 95°C 1000rpm in 2% SDC buffer (2% SDC, 100 mM Tris-HCl pH=8.5) and sonicated (Diagenode Bioruptor, 15 * 30s at high intensity). Protein concentration was determined via BCA Protein Assay (Thermo ...
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bioRxiv - Developmental Biology 2023Quote: ... Chromatin preparation continued as per Active Motif protocol except for the sonication steps which were performed in a Bioruptor® Plus sonication device by Diagenode with the following settings ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 % Triton X-100) supplemented with 1 x HALT in a 15 ml Bioruptor tube containing one scoop of sonication beads (Diagenode) and sonicated in Bioruptor Pico (Diagenode ...
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bioRxiv - Developmental Biology 2020Quote: ... All ChIPs were incubated overnight with the respective antibodies (CTCF Active Motif 61311; H3K27me3 Millipore 17-662; H3K27ac Diagenode C15410196; RAD21 abcam ab992) and precipitated after a 2h long incubation with magnetic beads (Dynabeads Protein A ...
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bioRxiv - Cell Biology 2023Quote: ... sonicated a minimum of 3 x 30 seconds (Diagenode sonicator), were optionally reduced with 100 mM dithiothreitol ...
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bioRxiv - Molecular Biology 2020Quote: ... 5 min total (Diagenode).
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bioRxiv - Genetics 2020Quote: ... 1.0% Triton X-100) was sonicated using a BioRuptor sonicator (Diagenode), followed by centrifugation at 16,000×g for 20 min at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... and sonicated using a Bioruptor (Diagenode, medium power, 3 x 15 sec). Protein concentration was measured using the Bradford assay (Bio-Rad) ...
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bioRxiv - Molecular Biology 2020Quote: ... 1% Triton X-100) and subjected to sonication using Bioruptor Standard (Diagenode). After centrifugation ...
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bioRxiv - Genomics 2020Quote: ... C15200211 (Diagenode, 5 μl/ChIP); H3K4me1 ...
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bioRxiv - Cancer Biology 2021Quote: ... sonicated 5 minutes (Bioruptor plus, Diagenode, 5 cycles high, 30 s on, 30 s off) and incubated at 37 °C for 60 min ...
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bioRxiv - Genomics 2020Quote: ... The lysates were sonicated with 5 × 5 min with 5s on/off intervals (Bioruptor, Diagenode) to get the DNA lengths between 200 and 1000 bp ...
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bioRxiv - Molecular Biology 2024Quote: ... Lysates were sonicated (5 times 5 min, 30 sec on/off) with a Bioruptor (Diagenode) and pelleted (13000 rpm ...
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bioRxiv - Molecular Biology 2024Quote: ... Lysates were sonicated (5 times 5 min, 30 sec on/off) with a Bioruptor (Diagenode) and pelleted (13000 rpm ...
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bioRxiv - Plant Biology 2019Quote: ... Five µg of genomic DNA were sonicated (Diagenode Bioruptor: 18 x 30 s) and denatured 10 min at 95°C in Buffer 1 (10 mM Tris HCl pH 7.5 ...
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bioRxiv - Biochemistry 2023Quote: ... for 5 min (Bioruptor Sonicator, Diagenode)73–75 ...
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bioRxiv - Genetics 2019Quote: ... we used ChIP grade antibodies specific to human H3K27ac histones (Diagenode). A negative control undergoing no IP (input ...
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bioRxiv - Genomics 2021Quote: ... Dynal Protein A magnetic beads (Diagenode) and ChIP-graded anti-myc antibody (ab9132 ...
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bioRxiv - Biochemistry 2023Quote: ... Unblocked protein A beads (Diagenode, C03020003) were used to pulldown the antibody-protein complexes ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were then sonicated (Bioruptor plus, Diagenode, 10 x 30s/30s ON/OFF cycles) and heated up to 37°C for 2 h while shaking (800 rpm ...
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bioRxiv - Cancer Biology 2021Quote: ... The DNA-protein-antibody complexes were captured by DiaMag protein A-coated magnetic beads (Diagenode; Cat#: C03010020) by incubating at 4 °C for 2 hours ...
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bioRxiv - Cancer Biology 2021Quote: ... The DNA-protein-antibody complexes were captured by DiaMag protein A-coated magnetic beads (Diagenode; Cat#: C03010020) by incubating at 4 °C for 2 hours ...
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bioRxiv - Molecular Biology 2019Quote: ... About 5 μg Ty1 antibody (C15200054; Diagenode), mouse IgG (12-371 ...
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bioRxiv - Neuroscience 2023Quote: ... and protein extraction beads (Diagenode, NJ, USA) according to the manufacturer instructions ...
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bioRxiv - Genomics 2019Quote: ... 10 mM EDTA (2 x 107 cell/ml) and sonicated in a Bioruptor device (Diagenode) for 25 min at 30 s intervals ...
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bioRxiv - Genetics 2021Quote: ... 7.5⍰x⍰106 cells were then homogenized and sheared by sonication using a Bioruptor (Diagenode) at high setting for 15⍰min ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were sonicated in 6 x 30 second intervals using a Bioruptor 300 Machine (Diagenode), then centrifuged for 10 minutes at 10,000g at 4°C ...
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bioRxiv - Genomics 2022Quote: ... by boiling at 95°C for 5 minutes followed by 5 sonication cycles of 30 s ON / 30 s OFF on the Bioruptor Pico sonicator (Diagenode) at 4°C ...
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bioRxiv - Molecular Biology 2024Quote: ... 0.1 % SDS in PBS) and sonicated 5 times for 5 min (30s ON/ 30s OFF) at high mode in a Bioruptor Pico (Diagenode). IP were performed overnight at 4°C using 1.5 mg of pre-coated epoxy Epoxy Dynabeads (M-270 ...
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bioRxiv - Molecular Biology 2019Quote: ... for 5 minutes with a Bioruptor Pico (Diagenode) to an average fragment size of ∼ 200 bp ...
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bioRxiv - Plant Biology 2019Quote: ... and sonicated 2 cycle x 30 second ON/ 30 second OFF using Bioruptor® Plus (Diagenode). After removal of cell debris by centrifugation (5 min ...
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bioRxiv - Genetics 2019Quote: ... Chromatin was sheared 10 x 30s ON/30s OFF with a Bioruptor Pico (Diagenode, Denville, NJ). For ChIP-qPCR ...
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bioRxiv - Neuroscience 2022Quote: ... Chromatin-protein complexes were immunoprecipitated with protein A/G magnetic beads and washed sequentially according to the manufacturer (Diagenode, C01010055). DNA fragments were then purified using IPure beads v2 (Diagenode ...
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bioRxiv - Developmental Biology 2019Quote: ... overnight and Protein A magnetic beads (Diagenode C03010020) the following day for 2 hours ...
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bioRxiv - Cancer Biology 2022Quote: ... homogenization was performed using protein extraction beads (Diagenode) and sonication in the lysis buffer followed by centrifugation to remove solid remains (Diagenode Bioruptor Plus sonicator manufacturer’s protocol for protein extraction from tissue fragments) ...
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bioRxiv - Developmental Biology 2019Quote: ... ChIP was performed using Protein G beads (Diagenode) O/N using a H3K27ac antibody (Active Motif ...
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bioRxiv - Microbiology 2023Quote: ... magnetic beads coated with DiaMag protein A (Diagenode), and antibodies against epitope tags (HA or MYC ...
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bioRxiv - Cell Biology 2020Quote: ... 600 ng of denatured DNA was incubated with 2 mg of 5-methylcytosine (5-mC) monoclonal antibody ([33D3] Diagenode, Cat No # C15200081) in IP buffer (0.1% SDS ...
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bioRxiv - Neuroscience 2019Quote: ... and immunoprecipitated with the antibody anti-5’-methylcytosine (Diagenode), following MagMeDIP kit settings ...
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bioRxiv - Immunology 2021Quote: ... and 5 µl Anti-GR antibody C15200010-50 (Diagenode), in a buffer with 1% Triton X-100 and 150 mM NaCl.
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bioRxiv - Cancer Biology 2024Quote: ... and of antibodies 5 μg/ml IgG (Diagenode, C15410206), anti-CCL2 (Novus Biologicals ...
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bioRxiv - Plant Biology 2021Quote: ... Protein extract was sonicated using a cooled Biorupter (Diagenode) for 10 minutes using high power with 30 seconds on 30 seconds off cycle ...
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bioRxiv - Plant Biology 2020Quote: ... magnetic beads (DiaMag protein A-coated magnetic beads: Diagenode) were incubated with each antibody overnight ...
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bioRxiv - Molecular Biology 2023Quote: ... Total proteins were sonicated on a Bioruptor Plus (Diagenode) (position high ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein extracts were finally sonicated in a Bioruptor (Diagenode) for 3 cycles at high intensity ...
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bioRxiv - Genetics 2022Quote: ... Immunoprecipitation was performed using 10 μg of chromatin and 2.5 μg of human antibody (H3K4me3 [RRID:AB_2616052, Diagenode C15410003], H3K4me1 [RRID:AB_306847, Abcam ab8895], and H3K27ac [RRID:AB_2637079, Diagenode C15410196]). Final DNA purification was performed using the GeneJET PCR Purification Kit (Thermo Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... CTCF ChIP-seq samples were prepared with 3 x 106 cells and 1 µg CTCF antibody (Diagenode C15410210). ChIP-seq Dilution Buffer (16.7 mM Tris-HCl pH 8.1 ...