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Citations for Diagenode :
251 - 283 of 283 citations for Herpes Simplex Virus 1 Lysate Antigen since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2019Quote: ... The digestion was then stopped with 5mM EDTA and the digested chromatin was solubilized in 1% SDS and three pulses of 30sec sonication using a Bioruptor ultrasonic cell disruptor (Diagenode). 200µg of pre-cleared chromatin was immunopurified with incubation of 30µl of Anti-FLAG M2 Affinity Gel (Sigma ...
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bioRxiv - Molecular Biology 2019Quote: ... SDS was then added to a final concentration of 1% followed by a 3x 30sec sonication steps with a bioruptor sonicator (Diagenode). Insoluble precipitates were removed by centrifugation and soluble crosslinked chromatin extracts were diluted 10x in IP buffer (0.3M Sucrose ...
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bioRxiv - Neuroscience 2020Quote: ... PFFs are diluted in PBS and sonicated for 10 cycles (1 sec ON, 30 sec OFF, high intensity) in a bath sonicator at 10°C (BioRuptor; Diagenode).
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Checkpoint adaptation in repair-deficient cells drives aneuploidy and resistance to genotoxic agentsbioRxiv - Cell Biology 2019Quote: ... Sonification was performed for 10 sec at level 1 using “constant” mode with a 3 mm sonification tip (Branson) or in a Bioruptor Pico (Diagenode) for 2 cycles 30 sec each ...
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bioRxiv - Molecular Biology 2021Quote: ... cells were cross-linked for 10 min at room temperature with 1% formaldehyde before lysis followed by sonication of their chromatin (Bioruptor Plus, Diagenode) to obtain DNA fragments of 200–400 bp ...
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bioRxiv - Plant Biology 2023Quote: ... Nuclei were lysed in NLB (50 mM Tris-HCl pH8, 10 mM EDTA, 1% SDS and 1x Proteases Inhibitor cocktail. Chromatin was sonicated using a Diagenode Bioruptor (40 Hz ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were lysed on ice for 10 minutes prior to sonication for either 20 cycles (replicate 1) or 40 cycles (replicate 2) of 30s on / 30s off with a Bioruptor UCD-200 (Diagenode) at maximum power ...
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bioRxiv - Bioengineering 2023Quote: ... Membranes were blocked using 5% BSA in 1X TBST and incubated overnight with primary antibody (anti-Cas9; 1:1000 dilution, Diagenode #C15200216 ...
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bioRxiv - Neuroscience 2023Quote: ... Overnight incubation with primary antibodies at 4°C was performed using the following antibodies: Cas9 (Diagenode, C15200230-100, 1:500), alpha synuclein (Abcam ...
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bioRxiv - Cell Biology 2023Quote: ... the pellets were resuspended in 300 μl ChIP lysis buffer containing 1 mM PMSF and sonicated for 15 min (30s ON–60s OFF) in a Bioruptor (Diagenode). The lysates were centrifuged at 7000 rpm for 15 min at 4°C ...
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bioRxiv - Plant Biology 2023Quote: ... The remaining pellet was re-suspended in 300 µl of lysis buffer containing 1% SDS and sonicated using a bioruptor (Diagenode) at 4°C (15 sec ON/OFF for 5-min and repeated for 5 cycles) ...
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bioRxiv - Cancer Biology 2023Quote: ... were lysed in buffer (50 mM Tris-HCl, pH 7.8) containing 1% SDS and 0.1 M dithiothreitol and sonicated (Diagenode Bioruptor Plus). Number of sonication cycles was established based on a visual sample clarity assessment ...
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bioRxiv - Genomics 2023Quote: ... The immunoprecipitated DNA and 1% of the total input were reverse-crosslinked and eluted using the iPure v2 kit (Diagenode). For library preparation ...
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bioRxiv - Neuroscience 2023Quote: ... Cell pellet was resuspended in ice-cold lysis buffer (1xPBS, 2 mM PMSF, 2 mM DTT, 1 x protease inhibitors (Pierce)) and sonicated (Bioruptor Pico, Diagenode) at 4LJ with alternating 10 s burst/10 s break ...
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bioRxiv - Genomics 2023Quote: ... Preclearing 30μl of Dynabeads protein A/G for 1 h at 4 °C was followed by incubation with antibodies (H3K27ac, Diagenode, 3C15410196 ...
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bioRxiv - Cancer Biology 2019Quote: ... 1 × 107 cells / immunoprecipitation were fixed for 30 minutes (min) at room temperature (RT) using Diagenode Crosslink Gold (Diagenode, Liège, Belgium). After two washing steps with PBS (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2022Quote: Genomic DNA (1 μg) was fragmented to 300–600 bp after 25 cycles of 30 s of pulsed sonication in Bioruptor (Diagenode, Belgium). Fragmented DNA was end-repaired and adapter ligation was performed using the NEBNext Ultra II DNA Library Prep Kit (New England Biolabs ...
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bioRxiv - Developmental Biology 2019Quote: ... Embryos were kept on ice and processed directly or frozen at −80C in 20 ml of RNAse/DNAse free PBS supplemented with 1× protein inhibitor (Diagenode, Belgium). Embryos younger than late gastrula (younger than E7.5 ...
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bioRxiv - Cell Biology 2021Quote: ... 1 mM PMSF, pH 8.5) and sonicated for 5 min (30 sec on, 1 min off) with Bioruptor UCD-200TM (Diagenode, Liège, Belgium). The resulting cell lysates were centrifuged ...
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bioRxiv - Cell Biology 2023Quote: ... Each chromatin sample containing about 6,000 cells was incubated with 1 µg of anti-H3K27me3 antibody (Diagenode, C15410195, lot. A1811-001P). ChIP was performed using the automated platform SX-8G IP-Star platform (Diagenode ...
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bioRxiv - Cell Biology 2023Quote: ... in 10 µl nuclease-free H2O and amplified with NEBNext® High-Fidelity 2X PCR Master Mix and 1 ul of primer pair from Diagenode (24 SI for tagmented libraries ...
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bioRxiv - Plant Biology 2023Quote: ... and cOmplete protease inhibitor cocktail tablets) and proceeded for sonication to shear the chromatin (∼ 500 bp to 1 kb size fragments) using Bioruptor Plus (Diagenode, USA) set at low mode and 45 cycles of 30 sec ON ...
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bioRxiv - Genomics 2023Quote: ... Preclearing 30μl of Dynabeads protein A/G for 1 h at 4 °C was followed by incubation with antibodies (H3K27ac, Diagenode, 3C15410196; H3K4me3, Diagenode 3C15410003). Reverse-crossed IP sample were pulled down with streptavidin C1 beads (Life Technologies) ...
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bioRxiv - Developmental Biology 2021Quote: ... 1 mM PMSF) and chromatin was sheared by sonication to approximately 500-100 bp fragments using the Bioruptor® sonicator (Diagenode, Belgium). Immunoprecipitation was done with the anti-H3K27me3 (Active-motif ...
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bioRxiv - Microbiology 2021Quote: 60,000 iATII cells or Calu-3 cells were incubated for 1 h at 4°C with equal protein concentrations of control rabbit IgG (Diagenode, Cat#C15410206) or 1/200 dilution of rabbit anti-ACE2 (Abcam ...
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bioRxiv - Plant Biology 2023Quote: ... Slides were blocked with 4% BSA for 30 minutes at 37°C in a moist box followed by primary (anti-H3K9m2, C15410060, Diagenode, 1:500) and secondary (Alexa Fluor 488 Goat anti-Rabbit IgG1 Secondary Antibody ...
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bioRxiv - Molecular Biology 2021Quote: ... The membranes were incubated with blocking buffer (5% milk powder in TBST) for one hour at room temperature and afterwards incubated with primary antibody (anti-H3K27me3, Diagenode # C15410195, 1:1000) in blocking buffer overnight at 4 °C ...
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bioRxiv - Genomics 2022Quote: ... libraries were constructed with 200 ng input DNA from each sample (n = 24; Table 1) using the Premium RRBS Kit (Diagenode, Cat. No. C02030032) according to manufacturer instructions ...
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bioRxiv - Neuroscience 2022Quote: ... PFFs were diluted in 1× PBS to a final concentration of 100 μL/Ml and sonicated with Bioruptor sonication device (Diagenode, Liege, Belgium, #B01020001) at the following settings ...
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bioRxiv - Molecular Biology 2022Quote: ... 100 mM TEAB and 1 x Halt protease inhibitor cocktail) and sonication (3 × 5 min cycles of 30s on/off; Diagenode Bioruptor sonication device). Extracted proteins protein were digested to peptides with in-solution digestion performed at the Turku Proteomics Facility ...
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bioRxiv - Molecular Biology 2019Quote: ... cells were crosslinked with 1% PFA at room temperature for 10 minutes and sonicated for 11 cycles (30 s ON/OFF, Diagenode Bioruptor Pico bath sonicator). 5% of the lysate was reserved as Input ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.1% SDS and 0.5% N-lauroylsarcosine) and the chromatin was fragmented by sonication for 10 cycles on Bioruptor (Diagenode high settings, 30’’ on/off). Chromatin was separated from cell debris using centrifugation and stored at -80C for maximum of 2 months.
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bioRxiv - Neuroscience 2024Quote: ... The nuclear pellets were lysed with lysis buffer (50 mM Tris-HCl, 10 mM EDTA, 1% SDS, pH 8) and then sonicated with Bioruptor (Diagenode, 15 cycles, 30s ON/30s OFF). The size of the DNA in the sheared chromatin fragments was checked before precipitation by a Tapestation system (Agilent 4200 ...