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Citations for Diagenode :
1 - 50 of 104 citations for Glycine N Fmoc 2 13C 99%;15N 98% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... 2 μl of precleared chromatin was added to 98 μl buffer iE1 (Diagenode). To samples and inputs ...
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bioRxiv - Genomics 2021Quote: ... Binding was stopped by adding 57 µL 2 M glycine (Diagenode, cat. C01011000) and incubating the sample for 5 min at room temperature ...
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bioRxiv - Cancer Biology 2020Quote: ... The fixation reaction was quenched with glycine solution (Diagenode) and incubated at room temperature for 5 minutes ...
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bioRxiv - Microbiology 2023Quote: ... at room temperature (RT) for 30min and then quenched with glycine 0.125M (C01010062, Diagenode) at RT for 10min ...
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bioRxiv - Molecular Biology 2022Quote: ... Reactions were stopped with glycine followed by nuclei isolation and chromatin shearing using a Bioruptoptor pico (Diagenode). Chromatin was immunoprecipitated overnight at 4°C with the following antibodies ...
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bioRxiv - Cell Biology 2024Quote: ... by incubation for 5 min at 99°C followed by sonication (alternating 30 s on- and 30 s off-cycles at highest intensity output (Bioruptor, Diagenode)) ...
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bioRxiv - Immunology 2020Quote: ... The cells were cross-linked with 1% formaldehyde for 10 minutes at RT and quenched with 2.5 M glycine (Diagenode) for 5 minutes at RT ...
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bioRxiv - Cancer Biology 2024Quote: ... Crosslinking was terminated by the addition of 1/10 volume 1.25 M glycine for 5 min at room temperature followed by cell lysis and sonication (Bioruptor, Diagenode), resulting in an average chromatin fragment size of 200 bp ...
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bioRxiv - Genomics 2023Quote: ... 0.5% N-lauroylsarcosine) and sonicated using Bioruptor Pico (Diagenode) in 15mL tubes for 20 cycles of 30 s ON and 30 s OFF pulses in 4°C bath ...
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bioRxiv - Developmental Biology 2022Quote: ... in VFG media for 10 minutes at room temperature followed by a 5-minute quench with glycine (in True MicroChIP kit, Diagenode) at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were incubated O/N with 0.25µg of H3K9me3 (C15410193-Diagenode).
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bioRxiv - Cell Biology 2022Quote: ... and 0.5% N-lauroylsarcosine) and sonicated in a water bath sonicator (Diagenode Bioruptor ...
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bioRxiv - Genomics 2021Quote: ... while Lab 2 used Antibody 2 (Diagenode, Denville ...
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bioRxiv - Molecular Biology 2019Quote: ... 0.5% N-lauroylsarcosine) and sonicated for 30 minutes using a BioRuptor Pico (Diagenode), shearing genomic DNA to an average size of 0.5 kb ...
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bioRxiv - Molecular Biology 2022Quote: ... supplemented with 0.5% SDS and 0.2% n-lauroylsarcosine and sonicated using a Bioruptor (Diagenode) with high power settings to obtain an average fragment size distribution of 200-500 bp ...
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bioRxiv - Molecular Biology 2021Quote: ... with following appropriate antibodies at 4 °C o/n a rotating wheel: H3K27me3 (Diagenode, C15410195), RING1B (Cell Signaling ...
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bioRxiv - Genomics 2023Quote: ... a Megaruptor 2 (Diagenode) was used to shear 3 μg of DNA to 15kb fragments ...
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bioRxiv - Molecular Biology 2021Quote: ... 0.5% N-lauroylsarcosine) and sonicated for 25 cycles (30 sec on/off) using a BioRuptor Pico sonicator (Diagenode), shearing genomic DNA to produce fragments between 300bp and 1kb ...
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bioRxiv - Cell Biology 2023Quote: ... H3K27me3 (Diagenode; #C15410195; 2 μg) and IgG-rabbit (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... H3K9me3 (Diagenode; #C15410193; 2 μg), H3K27me3 (Diagenode ...
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The G2-phase enriched lncRNA SNHG26 is necessary for proper cell cycle progression and proliferationbioRxiv - Molecular Biology 2021Quote: ... 2 μg anti-H3K27me3 (Diagenode, C15410195), and 2.5 μg anti-enhancer of zeste homolog 2 (EZH2 ...
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bioRxiv - Cell Biology 2024Quote: ... 2 µg of 5mC antibody (Diagenode) were added to the sample and incubated for 2 h at 4°C with rotation ...
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bioRxiv - Cancer Biology 2021Quote: ... sodium deoxycholate and 0.5% (vol/vol) N-lauroylsarcosine) and the chromatin was sheared to 300 – 800 base pair (bp) fragments by Bioruptor (Diagenode, Belgium) 12 cycles of 30 second “ON” and 30 second “OFF” intervals ...
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bioRxiv - Cell Biology 2020Quote: ... anti-H4K20me3 (2 μl/ChIP, C15410207, Diagenode), anti-PA28γ (0.5 μl /ChIP ...
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bioRxiv - Cell Biology 2020Quote: ... anti-H3K9me3 (2 μl/ChIP, C15410056 Diagenode), anti-H3K4me3 (1 μl/ChIP ...
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bioRxiv - Microbiology 2021Quote: ... gDNA was sheared using Megaruptor 2 (Diagenode) to an average size of 8.9kb ...
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bioRxiv - Genomics 2021Quote: ... or 2 µg IgG control (Diagenode C15410296) was used ...
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bioRxiv - Genomics 2021Quote: ... and sheared DNA was added to antibodies coupled to washed magnetic beads (anti-FLI1 2 µg, ab15289, Abcam; anti-ERG 2 µg, ab92513, Abcam; anti-H3K4me3 1.4 µg, C15410003, Diagenode; H3K27me3 2.9 µg ...
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bioRxiv - Biochemistry 2023Quote: ... supplemented with 2% (v/v) 2-mercaptoethanol and samples were sonicated using a Bioruptor sonicator (Diagenode SA, Seraing, Belgium) at high amplitude for five 30 s on and off cycles ...
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bioRxiv - Immunology 2023Quote: ... 0.1% deoxycholate and 0.5% N-lauroylsarcosine) and split into two in 15ml polypropylene tubes suitable for the Bioruptor® Pico (Diagenode, Catalog # C01020031). One lid of a 1.5ml tube was filled with sonication beads and added to each sample ...
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bioRxiv - Neuroscience 2023Quote: ... Cell pellet was resuspended in ice-cold lysis buffer (1xPBS, 2 mM PMSF, 2 mM DTT, 1 x protease inhibitors (Pierce)) and sonicated (Bioruptor Pico, Diagenode) at 4LJ with alternating 10 s burst/10 s break ...
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bioRxiv - Microbiology 2021Quote: ... but rather using the Megaruptor 2 system (Diagenode) with the software settings at 20Kb and following their user manual ...
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bioRxiv - Genetics 2022Quote: ... 0.5 μg (2 ul) of H3K9me3 antibody (Diagenode) and 0.5 μL of biotin-myc antibody (Abcam ...
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bioRxiv - Genomics 2023Quote: ... Size selection was performed with Megaruptor 2 (Diagenode) with default settings ...
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bioRxiv - Genomics 2022Quote: ... libraries were constructed with 200 ng input DNA from each sample (n = 24; Table 1) using the Premium RRBS Kit (Diagenode, Cat. No. C02030032) according to manufacturer instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.1% SDS and 0.5% N-lauroylsarcosine) and the chromatin was fragmented by sonication for 10 cycles on Bioruptor (Diagenode high settings, 30’’ on/off). Chromatin was separated from cell debris using centrifugation and stored at -80C for maximum of 2 months.
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bioRxiv - Genomics 2020Quote: ... genomic DNA was sheared using the Megaruptor 2 (Diagenode) with a long hydropore and a 20 kb shearing protocol ...
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bioRxiv - Molecular Biology 2020Quote: ... 2 µg DNA was sonicated in a Bioruptor Plus (Diagenode). DamID adaptors were removed by AlwI digestion ...
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bioRxiv - Developmental Biology 2019Quote: ... subtracted to 2 cycles of sonication on a bioruptor (Diagenode) with 30 sec “ON”/“OFF” at low setting and the remaining cell debris was removed by centrifugation at 21,000 g for 10 min at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... boiled for 5 min at 95°C 1000rpm in 2% SDC buffer (2% SDC, 100 mM Tris-HCl pH=8.5) and sonicated (Diagenode Bioruptor, 15 * 30s at high intensity). Protein concentration was determined via BCA Protein Assay (Thermo ...
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bioRxiv - Genomics 2023Quote: ... The samples were fragmented to 10 kb using Megaruptor 2 (Diagenode). For each fragmented sample ...
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bioRxiv - Cell Biology 2023Quote: ... 2 μg of antibodies ChIP-grade (H3K4me2, Abcam ab32356; H3K4me3, Diagenode C15410003 ...
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bioRxiv - Genomics 2020Quote: Genomic DNA was sheared to 20 kb fragments using Megaruptor 2 (Diagenode) and size selected using the BluePippin system (Sage Science ...
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bioRxiv - Genomics 2020Quote: ... Genomic DNA was sheared to 8 kb fragments using Megaruptor 2 (Diagenode). Standard SMRTbell libraries were prepared using Template Preparation Kit 1.0 (Pacific Biosciences ...
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The G2-phase enriched lncRNA SNHG26 is necessary for proper cell cycle progression and proliferationbioRxiv - Molecular Biology 2021Quote: ... and 2.5 μg anti-enhancer of zeste homolog 2 (EZH2) (Diagenode, C15200180). Antibody-protein complexes were immunoprecipitated with protein A/G Dynabeads for 3 hours at 4°C with rotation ...
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bioRxiv - Plant Biology 2022Quote: ... Libraries were synthesized using a MicroPlex Library Preparation Kit (v.2; Diagenode). Libraries were sequenced as 2 × 50-bp reads on an Illumina HiSeq 2500 instrument ...
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bioRxiv - Cancer Biology 2023Quote: ... 2 ug/ul of lysates were sonicated with a Bioruptor Plus (Diagenode) for 20 cycles (30 seconds ON and 30 seconds OFF) ...
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bioRxiv - Genomics 2020Quote: ... Genomic DNA was fragmented to 20 kb using Megaruptor 2 (Diagenode, Liege, Belgium) and size selected with a 10 kb cut-off using the Blue Pippin system (Sage Science ...
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bioRxiv - Genomics 2021Quote: Genomic DNA was sheared to 20 kb fragments using the Megaruptor 2 (Diagenode) and size selected with a 10 kb cut-off using the BluePippin system (Sage Science) ...
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bioRxiv - Plant Biology 2022Quote: ... Genomic DNA was sheared to 20-kb fragments using Megaruptor 2 (Diagenode, Belgium) and then bead-size selected with AMPure PB beads (Pacific Biosciences ...