Labshake search
Citations for Diagenode :
251 - 300 of 308 citations for Dengue Virus Serotype 3 Envelope Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Genomics 2021Quote: ... Cells were then sonicated for 10 cycles of 30 seconds on/off on a Bioruptor Pico (Diagenode) at 4°C ...
-
bioRxiv - Genomics 2019Quote: Immunoprecipitations from 105 cells were performed using IP star robot at 4°C (indirect 200µl method, Diagenode) with an anti-BrdU antibody (10μg ...
-
bioRxiv - Plant Biology 2022Quote: ... Cells were ruptured by 5 × 15 sec sonication in an ice bath in Bioruptor UCD-200 (Diagenode) at medium intensity with 2 min breaks on ice ...
-
bioRxiv - Molecular Biology 2023Quote: ... Chromatin from 10 million cells was sonicated to between 200-600 bp using a Bioruptor Plus (Diagenode). 100µL of Dynabeads Protein G (Invitrogen ...
-
bioRxiv - Cancer Biology 2023Quote: ... cells were lysed and chromatin was prepared using the Chromatin EasyShear Kit - High SDS (Diagenode; Cat# C01020012), and shearing of the chromatin was achieved using the Bioruptor with four rounds of ten cycles of 30 seconds “ON„ and 30 seconds “OFF„ at HIGH power setting (position H) ...
-
bioRxiv - Genomics 2022Quote: ... U2OS cells treated with Nutlin-3a or DMSO solvent control were sonicated using a Bioruptor (Diagenode, Beligum) for 10 cycles (60 seconds on and 30 seconds off with high intensity ...
-
bioRxiv - Genomics 2020Quote: ... One µg per 100,000 cells of the desired antibody (H3K4me3 C15410003, H3K27ac C15410196, H3K27me3 C15410195, all from Diagenode) is added into each well ...
-
bioRxiv - Molecular Biology 2021Quote: ... Fixed cells were resuspended in 1 ml RIPA Binding Buffer and placed in the Bioruptor Pico (B01060010, Diagenode) for 10 cycles of 30 seconds on ...
-
bioRxiv - Biophysics 2020Quote: ... Cell lysis were performed using EmulsiFlex-C3 (Avestin Europe GmbH, Mannheim, Germany) and Bioruptor UCD-200 (Diagenode, Belgium). Inclusion bodies were recovered by centrifugation of cell lysate at 7,000 x g for 1 hour at 4°C and were resuspended in 20 mL denaturing binding buffer (20 mM Tris-Cl ...
-
bioRxiv - Cancer Biology 2022Quote: ... sonicated until the lysates were clear of long DNA and other visible cell debris (Diagenode Bioruptor Plus sonicator). Lysates were processed by the Multi-Enzyme Digestion Filter Aided Sample Preparation (MED FASP ...
-
bioRxiv - Cancer Biology 2020Quote: ... The sample was then incubated with antibodies (H3K27ac, Diagenode, C15410196; H3K27me3, Cell Signaling 9733S; H3K4me3, Diagenode C15410003 premium) coupled with protein A and protein G beads (Life Technologies ...
-
bioRxiv - Cell Biology 2023Quote: ... Cells were sonicated at high setting with 30 s on/off cycles using a Bioruptor UCD-200 (Diagenode). Lysates were cleared by centrifugation at 14000×g ...
-
bioRxiv - Molecular Biology 2021Quote: ... Subsequently, cells were spheroplasted according to the protocol from the Rando lab (Rando, 2010) followed by sonication (Bioruptor, Diagenode: ten cycles ...
-
bioRxiv - Genomics 2019Quote: ... Cell clumps were dissociated by mild sonication for 30 s using the medium intensity setting in a Bioruptor (Diagenode). Lysing enzymes from Trichoderma harzianum (Sigma ...
-
bioRxiv - Genomics 2021Quote: ... Cells were lysed in two steps with the Diagenode iDeal ChIP-seq kit for transcription factors (Diagenode, Seraing, Belgium).
-
bioRxiv - Genomics 2020Quote: ... For single crosslinked cells nuclei were sonicated for 4 cycles of 30s on/30s off using a Picoruptor (Diagenode). Immunoprecipitation was carried out overnight at 4°C using 2 μg of RUNX1 antibody (ab23980 ...
-
bioRxiv - Cell Biology 2021Quote: ... Cells were sonicated for 30 cycles at high setting (30s on, 30s off) using a Bioruptor Pico sonicator (Diagenode) and centrifuged at 13000 rpm for 10 min ...
-
bioRxiv - Cell Biology 2021Quote: ... Pelleted cells were resuspended in 1 ml 50 mM Tris pH 7.5 and sonicated in a Bioruptor Twin (Diagenode) on LOW 30 sec ON ...
-
bioRxiv - Genomics 2020Quote: ... then incubated with specific antibodies (anti-H3K27me3 Cell Signaling Technology 9733, anti-H3K36me2 CST 2901, anti-H3K27ac Diagenode C15410196), 1.5 million cells of sonicated cell lysate ...
-
bioRxiv - Cell Biology 2019Quote: ... or Raji iBZLF1 cells induced for 6 h were lysed in RIPA buffer and sheared with the BioRuptor (Diagenode) four times (5 min ...
-
bioRxiv - Microbiology 2021Quote: ... Cells were lysed by sonication (5 cycles, each 30 s on and 30 s off) using a Biorupter (Diagenode). A centrifugation step (at 4°C ...
-
bioRxiv - Immunology 2020Quote: ... The cells were cross-linked with 1% formaldehyde for 10 minutes at RT and quenched with 2.5 M glycine (Diagenode) for 5 minutes at RT ...
-
bioRxiv - Cancer Biology 2023Quote: ... Isolated nuclei from primary fallopian tube secretory epithelial cells bound to concalavin A beads were incubated with primary antibody that detects H3K27ac (0.7μg, Diagenode, RRID:AB_2637079) at 4℃ overnight ...
-
bioRxiv - Cell Biology 2023Quote: ... Cells were incubated with mixing for 30 min at 4°C before sonicating with a Bioruptor® Pico (Diagenode). Lysates were then spun for 10 min at 15,000 g ...
-
bioRxiv - Genomics 2023Quote: ... 7μL Tri-Methyl-Histone H3 (Lys27) (C36B11) Rabbit mAb (Cell Signalling Technology #9733) or 1μg negative control rabbit IgG (Diagenode) per ChIP reaction ...
-
bioRxiv - Genetics 2024Quote: ... Cells were lysed by sonication for 15 cycles (high, 30 seconds on, 30 seconds off) using Bioruptor Plus (Diagenode). After a 15-minute incubation on ice and centrifugation (21,000 ✕ g ...
-
bioRxiv - Systems Biology 2024Quote: ... cells were incubated on ice for 30 min and sonicated for five minutes (30 sec on/off, Diagenode Bioruptor). Afterwards ...
-
bioRxiv - Cancer Biology 2024Quote: ... Crosslinking was terminated by the addition of 1/10 volume 1.25 M glycine for 5 min at room temperature followed by cell lysis and sonication (Bioruptor, Diagenode), resulting in an average chromatin fragment size of 200 bp ...
-
bioRxiv - Molecular Biology 2021Quote: ... the cells were sonicated for 10 min at the high intensity setting (30 sec on/off) in a Bioruptor (Diagenode). The whole cell extract was adjusted to 25 mM MgCl2 and 5 mM CaCl2 and 1 µL DNAse I (30 U ...
-
bioRxiv - Cell Biology 2022Quote: ... The cell lysate was transferred to 15 mL Bioruptor Pico tubes along with ~200μL of the corresponding sonication beads (Diagenode C010200031) and sonicated using a Bioruptor Pico (Diagenode B01060010 ...
-
Pancreatic progenitor epigenome maps prioritize type 2 diabetes risk genes with roles in developmentbioRxiv - Genomics 2020Quote: ... Cells were lysed and homogenized using a Dounce homogenizer and the lysate was sonicated in a Bioruptor® Plus (Diagenode), on high for 3 × 5 min (30 sec on ...
-
bioRxiv - Genomics 2020Quote: ... Cells were lysed and homogenized using a Dounce homogenizer and the lysate was sonicated in a Bioruptor® Plus (Diagenode), on high for 3 x 5 min (30 sec on ...
-
bioRxiv - Molecular Biology 2020Quote: ... nucleic acids gently extracted from unfixed NTERA-2 cells were sheared by sonication (12 cycles at high power, 15s ON / 90s OFF) with a Bioruptor (Diagenode). 4.4 μg of sheared nucleic acids were incubated with 10 μg of S9.6 antibody for immunoprecipitation overnight at 4°C ...
-
bioRxiv - Genetics 2019Quote: 0.5 × 106 frozen cells were resuspended in 250 µl buffer iL1 with proteinase inhibitors cocktail (iDeal ChIP-seq Kit for Histones, Diagenode) and incubated for 10 min at 4ºC on the Bohemian wheel ...
-
bioRxiv - Neuroscience 2020Quote: ... the crosslinked cells were resuspended in 2 mL of lysis buffer and sonicated (in a cold room) by using a Bioruptor (Diagenode) with the following parameters ...
-
bioRxiv - Molecular Biology 2020Quote: ... with minor modifications: Cells were resuspended in PBS at 4°C after flow cytometry sorting and incubated with ChIP Crosslink Gold (Diagenode #C01019027 ...
-
bioRxiv - Molecular Biology 2019Quote: ... The digestion was then stopped with 5mM EDTA and the digested chromatin was solubilized in 1% SDS and three pulses of 30sec sonication using a Bioruptor ultrasonic cell disruptor (Diagenode). 200µg of pre-cleared chromatin was immunopurified with incubation of 30µl of Anti-FLAG M2 Affinity Gel (Sigma ...
-
bioRxiv - Developmental Biology 2019Quote: ... Cells were lysed and homogenised using a Dounce homogeniser and the lysate was sonicated in a Bioruptor® Plus (Diagenode), on high for 3 × 5 min (30 sec on ...
-
bioRxiv - Immunology 2021Quote: ... Chromatin was prepared from 40–50 million cells using Diagenode Chromatin shearing optimization kit (Cat no. C01010055) and further subjected to sonication using Bioruptor sonicator (Diagenode) to obtain chromatin fragments of 100–500 bps ...
-
bioRxiv - Genomics 2021Quote: ... ChIP-seq for H3K4me3 and H3K27ac in PEER cell line was generated using MicroPlex Library Preparation Kit (Diagenode, Liege, Belgium) according to the BLUEPRINT protocol (http://dcc.blueprint-epigenome.eu/#/md/methods) ...
-
bioRxiv - Cell Biology 2022Quote: ... cells were sonicated for 10 cycles of 30 sec “on” and 30 sec “off” at 4°C with Bioruptor (Diagenode) and the protein concentration was measured using Bradford protein assay (Thermo Fisher Scientific) ...
-
bioRxiv - Microbiology 2022Quote: ... Immunoprecipitation was done on chromatin corresponding to the remaining 10 000 cells using antibodies against H3K4me3 (Diagenode, cat no. C15410003) – 10% of the chromatin was used for input ...
-
bioRxiv - Molecular Biology 2022Quote: ... Cross-linked cell lysates were solubilized using a multi-beads shocker (Yasui Kikai) and sonicated using a Bioruptor UCD-200 system (Diagenode). The sheared samples were centrifuged at 20,000 g for 10 min at 4°C ...
-
bioRxiv - Genetics 2023Quote: ... Briefly, DNA from activated B cells (day 4 with IL-4, anti-CD40) was sonicated to ∼1000 bp (Diagenode Bioruptor) before amplification via an Sµ-specific biotinylated primer (5’/5BiosG/CAGACCTGGGAATGTATGGT-3’) ...
-
bioRxiv - Immunology 2023Quote: ... Cells were then lysed to isolate chromatin and sonicated for 10-20 min (depending on cell type) using a Bioruptor (Diagenode). Fragmentation of sonicated chromatin to lengths of 200-500 bp was confirmed by agarose gel electrophoresis.
-
bioRxiv - Biochemistry 2023Quote: ... Cell lysis was obtained by pulsed ultrasonication (5 cycles, 30” On, 30” Off, at High intensity using Diagenode Bioruptor 300). Lysates were incubated 10 minutes at 4°C with Dynabeads™ His-Tag Isolation (Invitrogen ...
-
bioRxiv - Biochemistry 2023Quote: ... Cell lysis was obtained by pulsed ultrasonication (10 cycles, 30” On, 30” Off, at High intensity using Diagenode Bioruptor 300), complete lysis was monitored by phase contrast microscopy ...
-
bioRxiv - Bioengineering 2024Quote: ... The cell lysates were heated at 95[ for 5 minutes and sonicated for 15 min using a Bioruptor Plus (Diagenode). The supernatant was collected after centrifugation at 18,000 g for 30 min at 4[ ...
-
bioRxiv - Genomics 2020Quote: ... The sheared chromatin derived from one million cells was subjected to magnetic immunoprecipitation and tagmentation using an SX-8G IP-STAR® Compact Automated System (Diagenode) with the anti-GATA6 antibody (D61E4 ...
-
bioRxiv - Cancer Biology 2019Quote: ... 1 × 107 cells / immunoprecipitation were fixed for 30 minutes (min) at room temperature (RT) using Diagenode Crosslink Gold (Diagenode, Liège, Belgium). After two washing steps with PBS (Thermo Fisher Scientific) ...