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Citations for Diagenode :
101 - 150 of 320 citations for Dengue Virus Serotype 2 Envelope Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2019Quote: ... Embryos were kept on ice and processed directly or frozen at −80C in 20 ml of RNAse/DNAse free PBS supplemented with 1× protein inhibitor (Diagenode, Belgium). Embryos younger than late gastrula (younger than E7.5 ...
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bioRxiv - Genetics 2021Quote: ... 3 to 7 days later individual colonies were picked and expanded and western blot was used to confirm Cas9 protein expression (Diagenode, C15200203). Cas9 expression in a high expressing line was confirmed by immunohistochemistry (5).
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bioRxiv - Genomics 2023Quote: ... Preclearing 30μl of Dynabeads protein A/G for 1 h at 4 °C was followed by incubation with antibodies (H3K27ac, Diagenode, 3C15410196; H3K4me3, Diagenode 3C15410003). Reverse-crossed IP sample were pulled down with streptavidin C1 beads (Life Technologies) ...
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bioRxiv - Biochemistry 2021Quote: Cellular human and viral material in sputum deposited on glass slides was lysed in 50 μl 2% SDS dissolved in 50 mM Tris/HCl (pH 8.2) followed by sonication in a Bioruptor Pico (Diagenode). Proteins were digested using the SP3 protocol as described below.
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bioRxiv - Cell Biology 2021Quote: ... DNA was fragmented with 2 rounds of 30 cycles 30sec ON-30sec OFF of sonication with Bioruptor Plus (Diagenode) set on HIGH and purified with Qiagen MinElute kit (Qiagen) ...
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bioRxiv - Microbiology 2022Quote: ... The DNA was sheared to a modal size of 10 kb using a Megaruptor 2 (Diagenode Diagnostics, Seraing (Ougrée), Belgium) ...
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bioRxiv - Molecular Biology 2022Quote: ... 2 mg of chromatin lysate was rotated overnight at 4°C with 5 μg antibody: rabbit IgG (Diagenode #C15410206), anti-NIPBL (NIPBL#1 ...
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bioRxiv - Molecular Biology 2023Quote: ... All samples were lysed the same day 2 weeks before proteomics analysis in 100µL iST-NHS-Lysis buffer (PreOmics GmbH) by sonication (Bioruptor (DIagenode) 15s ON/15s OFF for 15 min) ...
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bioRxiv - Microbiology 2024Quote: ... Immunoprecipitation was done with 2 µg of chromatin and 4 µg of antibody against H3K9me3 (Diagenode, C15410193, lot A2217P). Sequencing libraries were prepared from 5 ng of immunoprecipitated DNA as previously described [19 ...
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bioRxiv - Microbiology 2023Quote: ... 10 μg total RNA was incubated overnight at 4 °C with Dynabeads™ Protein G magnetic beads pre-bound with anti-Ψ (Diagenode) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... Samples were split into 2 x 1.5 mL in 15 mL polypropylene tubes suitable for the Bioruptor® Pico (Diagenode). BioRuptor Sonicator settings ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were lysed and sonicated in the presence of 0.2% SDS for 30 cycles (30 s on, 30 s off) using the high setting on a Bioruptor sonicator (Diagenode). Cell debris were pelleted by centrifugation and SDS in the lysate supernatants was diluted to 0.07% ...
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FOXO dictate initiation of B cell development and myeloid restriction in common lymphoid progenitorsbioRxiv - Immunology 2022Quote: ChIPseq was performed as previously described (Bouderlique et al., 2019) using H3K27Ac antibodies (Diagenode Cat #C15410196, lot A1723-0041D/2) and the ThruPLEX DNA-seq (Rubicon Genomics ...
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bioRxiv - Genomics 2020Quote: ... Nuclei were lysed for 15 min on ice with occasional inversion every 2 min and then sonicated for 24 cycles with 30s on and 30s off using Bioruptor (Diagenode). After sonication ...
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bioRxiv - Developmental Biology 2020Quote: ... Sonication was performed by splitting each sample to 2 Bioruptor tubes with sonication beads and using the Bioruptor Pico (Diagenode) for 18 cycles of 30 sec on and 30 sec off into an average size of approximately 200 bp ...
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bioRxiv - Microbiology 2020Quote: ... The supernatant was transferred to a sterile 2 ml microcentrifuge tube and sonicated with the Bioruptor UCD-200 sonicator (Diagenode) for 1 min on the low setting (160W at 20kHz ...
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bioRxiv - Genomics 2020Quote: ... high molecular weight DNA was diluted to a concentration of 25 ng/uL and fragmented to an average peak size of 25 kb using a Megaruptor-2 instrument (Diagenode). Small fragments <1 kb were removed and the DNA concentrated using bead purification (0.4 x volumes of Ampure-XP beads ...
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bioRxiv - Biochemistry 2022Quote: ... incubated at 95°C for 5 min and sonicated at 4°C for 2 cycles of 20 s at high intensity level using a BioRuptor (Diagenode). Samples were clarified by centrifugation at 16,000 x g for 15 min ...
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bioRxiv - Developmental Biology 2019Quote: ... and sonication for 2 cycles of 15s ON and 15s OFF on medium power at 4°C on a PicoBioruptor (Diagenode). After 10 minutes incubation at 4°C ...
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bioRxiv - Developmental Biology 2019Quote: ... and sonicated for 2 cycles of 15s ON and 15s OFF on full power at 4°C on a Bioruptor (Diagenode). Samples were incubated for 1 hour on a rotating wheel at 4°C and centrifuged 10 minutes at 12,000 g and 4°C ...
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bioRxiv - Immunology 2021Quote: ... 2 µg of antibody was used per reaction. H3K9me3 (Lot. A2217P) and H3K4me3 (Lot No. A1052D) antibodies were from Diagenode. Immunoprecipitated and input DNA were extracted by phenol-chloroform extraction and used for qPCR with SsoAdvanced Universal SYBR Green supermix (Biorad) ...
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bioRxiv - Molecular Biology 2022Quote: ... DNA was purified using phenol/chloroform and 2 µg of purified 3C sample was sonicated using the Bioruptor Pico (Diagenode). Biotinylated DNA was captured using MyOne Streptavidin T1 magnetic beads (Life Technologies ...
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bioRxiv - Neuroscience 2023Quote: ... α-Synuclein PFFs were vortexed and diluted with DPBS to 2 mg/mL and sonicated in a cooled bath sonicator at 9°C (Diagenode Bioruptor® Pico ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were lysed on ice for 10 minutes prior to sonication for either 20 cycles (replicate 1) or 40 cycles (replicate 2) of 30s on / 30s off with a Bioruptor UCD-200 (Diagenode) at maximum power ...
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bioRxiv - Cell Biology 2023Quote: ... which were incubated overnight on a rotatory wheel at 4°C with the primary antibodies H4K20me1 (Diagenode; #C15410034; 2 μg), H3K9me3 (Diagenode ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were lysed using a Bioruptor (Diagenode) and after centrifugation ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were lysed in a Bioruptor (Diagenode) for 10 min with cycles of 30 sec ON and 30 sec OFF ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were sonicated using a Bioraptor (Diagenode) in sonication buffer (10 mM Tris-Hcl pH 8.0 ...
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bioRxiv - Microbiology 2020Quote: ... Cells were disrupted in a sonicator (Diagenode) and subsequently centrifuged at 4°C (10,000 ×g for 15 min) ...
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bioRxiv - Microbiology 2021Quote: ... Cells were sonicated using the Bioruptor (Diagenode) for 30 cycles ...
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bioRxiv - Microbiology 2023Quote: ... Cells were lysed in a Bioruptor (Diagenode) (set to high ...
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bioRxiv - Neuroscience 2021Quote: ... equal volume of sheared chromatin was incubated overnight with 3 μg of antibody in iC1b buffer with protease inhibitors and BSA and washed DiaMag Protein A-coated magnetic beads (Diagenode Cat# C03010020-220) on a tube rotator at 4°C overnight ...
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bioRxiv - Microbiology 2020Quote: ... was added to the lysate and incubated at 37 °C for 10 min before sonication (2 × 8 bursts of 30 sec on ice using a Diagenode Bioruptor) to shear DNA fragments to a length of 300 to 500 bp ...
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bioRxiv - Microbiology 2022Quote: ... were added to the lysate and incubated at 37 °C for 10 min before sonication (2 x 8 bursts of 30 sec on ice using a Diagenode Bioruptor) to shear DNA fragments to a length of 300 to 500 bp ...
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bioRxiv - Biochemistry 2022Quote: ... heated to 95°C for 10 min shaking (1000 rpm) followed by 2 cycles à 20 s of sonication in BioRuptor (Diagenode). Aliquots containing 50 μg protein were transferred into a cartridge and cooled ...
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bioRxiv - Genomics 2022Quote: ... HMW-DNA (25 ng/μl) was separately sheared with 30 kb and 35 kb settings using a Megaruptor 2 instrument (Diagenode SA). However ...
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bioRxiv - Molecular Biology 2019Quote: ... 2% Triton-100 with protease inhibitors) and sonicated using a Bioruptor Pico (Diagenode, 3× cycles 30 sec on / 30 sec off). Sonicated lysates were diluted 2× in 50mM Tris ...
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bioRxiv - Molecular Biology 2019Quote: ... 2% Triton-100 with protease inhibitors and sonication using a Bioruptor Pico (Diagenode, 3× cycles 30 sec on / 30 sec off). 200μg of proteins for each sample were diluted to 1 ml with CoIP Lysis Buffer and incubated with 30μl of anti-FLAG M2 Magnetic Beads (Sigma M8823 ...
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bioRxiv - Plant Biology 2022Quote: ... Approximately 2 ug of genomic DNA was fragmented into 200-300 bp using the Bioruptor UCD-200 sonicator (Diagenode, Denville, NJ). Libraries were constructed using the DNA library prep kit for BGI platform (BGI ...
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bioRxiv - Microbiology 2024Quote: ... were added to the lysate and incubated at 37°C for 10 min before sonication (2 x 8 bursts of 30 sec on ice using a Diagenode Bioruptor) to shear DNA fragments to a length of 300 to 500 bp ...
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bioRxiv - Genomics 2024Quote: DNA concentration was normalized to 50 ng/μl and sheared to 6 Kb fragments using Megaruptor® 2 (Diagenode, Seraing, Belgium). Shearing quality was estimated with TapeStation 4200 (Agilent Technologies ...
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bioRxiv - Molecular Biology 2020Quote: ... Zirconium beads were removed from the cell lysate by centrifugation and the entire cell lysate was subject to sonication using the Bioruptor-Pico (Diagenode) for 3×10 cycles of 30 seconds ON/OFF each resulting in a final median size of chromatin fragments of 200 bp ...
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bioRxiv - Immunology 2020Quote: ... cells were resuspended in 2mL cell isolation buffer (IBc) in a 15mL conical and disrupted using a Bioruptor sonicator (Diagenode) with metal probe adaptor and short one-second pulses on “HI” setting ...
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bioRxiv - Cancer Biology 2021Quote: ... Lysed cells were sonicated with a Bioruptor (Diagenode) to obtain chromatin fragments (∼200–500 bp ...
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bioRxiv - Cancer Biology 2021Quote: ... Lysed cells were sonicated with a Bioruptor (Diagenode) to obtain chromatin fragments (~200–500 bp ...
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REEP4 is recruited to the inner nuclear membrane by ELYS and promotes nuclear pore complex formationbioRxiv - Cell Biology 2020Quote: ... cell lysates were sonicated in a BioRuptur (Diagenode) for 10 intervals of 30 seconds high power ...
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bioRxiv - Developmental Biology 2020Quote: ... Cells were sonicated on the Bioruptor Plus (Diagenode), 30s on/off for five cycles at high power ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were sonicated using the Bioruptor Pico (Diagenode) sonication device for 10 cycles (20s ON/40s OFF) ...
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bioRxiv - Bioengineering 2022Quote: ... Cells were lysed on a Bioruptor sonicator (Diagenode), using 10 cycles of sonication (45 sec ON ...
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bioRxiv - Molecular Biology 2021Quote: ... The cell lysate was sheared with Biorupter (Diagenode) with 3 cycles of 30 seconds ON/OFF for 10 min each at high power ...